Research Article |
Corresponding author: Santi Watiroyram ( santi.watiroyram@npu.ac.th ) Academic editor: Danielle Defaye
© 2017 Santi Watiroyram, La-orsri Sanoamuang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Watiroyram S, Sanoamuang L (2017) A new species of Mongolodiaptomus Kiefer, 1938 from northeast Thailand and a key to the species (Crustacea, Copepoda, Calanoida, Diaptomidae). ZooKeys 710: 15-32. https://doi.org/10.3897/zookeys.710.13941
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This study describes the new species Mongolodiaptomus loeiensis sp. n. collected from a temporary pond nearby a cave located in Loei Province, in northeastern Thailand. Mongolodiaptomus loeiensis sp. n. is similar to M. calcarus (Shen & Tai, 1965) in the male but can be distinguished from its congeners by the following unique characteristics in the males: (1) the right caudal ramus has 3 ventral chitinous prominences; (2) intercoxal plate of P5 is produced into 2 spine-like lobes on distal margin; (3) the basis of right P5 has a subglobular chitinous prominence on mid-distal caudal surface; and (4) the principal lateral spine on the right Exp-2 P5 is extremely bent at its tip. The occurrence of diaptomid copepods in the study area is discussed and an identification key to worldwide species of the genus Mongolodiaptomus Kiefer, 1938 is presented herein.
Copepoda , Diaptomidae , Loei Province, new record, rare species, southeast Asia, taxonomy
The freshwater calanoid copepods have been intensively studied in Thailand especially in northeast Thailand. However, their study in the Loei Province in the upper northeast region has so far been largely neglected. According to
The genus Mongolodiaptomus Kiefer, 1938 was defined by
To date, 37 diaptomid species are known from inland waters of Thailand (
Samples were collected using a plankton net with a mesh size of 60 µm. The copepod samples were transferred into 120 ml plastic bottles and preserved in 70% ethanol. In the laboratory, samples were selected for individual adults and placed in a mixture of glycerol and 70% ethanol (ratio ~ 1:10 v/v) under a stereomicroscope at 40× magnification. Specimens were transferred to pure glycerol and dissected at 40–100-× magnification under an Olympus SZ51 stereomicroscope.
All appendages and body ornamentation were examined at1000-× magnification. All the drawings were made at the same magnification (1000-×), with a drawing tube mounted on an Olympus compound microscope (CX31). The final versions of the drawings were made using the CORELDRAW® 12.0 graphic program. For permanent slides, all body parts were put in a drop of glycerol on a microscope slide, covered by a cover glass, and sealed with nail polish.
Specimens for a scanning electron microscopy (SEM) were dehydrated in an ethanol series (50%, 70%, 80%, 90%, 95%, 100%, and 100%) for 15 min each concentration. After dehydration, specimens were dried in a critical point dryer using liquid carbon dioxide as the exchange medium. Dried specimens were mounted on stubs using adhesive tape under a stereomicroscope. Then, specimens were coated with gold in a sputter-coater. The SEM photographs were carried out using a scanning electron microscope (FEI Helios NanoLab G3 CX).
Enp endopod;
Exp exopod;
Exp/Enp-n exopodal segment n/endopodal segment n;
P1–P5 swimming legs 1–5.
The nomenclature and descriptive terminology follow
A temporary pond nearby the Prakaipetch Cave, Nadokkham Subdistrict, Na Duang District, Loei Province, northeastern Thailand; 17°54'23"N, 101°54'23"E; altitude: 420 m above sea level.
One adult male, NHMUK 2017.134, dissected and mounted in glycerol on one slide: collected on 5 August 2015 by S. Watiroyram.
One adult female, NHMUK 2017.135, dissected and mounted in glycerol on one slide: collected on same date by the same collector.
Ten adult females and males, NHMUK 2017.136–145, undissected and preserved in 70% ethanol in 1.5 ml microtube; 10 adult females and males, NPU 2017–001, undissected and preserved in 70% ethanol in 1.5 ml microtube: collected on same date by the same collector.
The right P5 Exp-2 of male in Mongolodiaptomus loeiensis sp. n. with principal spine slightly posterior to mid-outer margin and one spinous process each proximally and distally, fits the diagnostic features of the genus Mongolodiaptomus sensu
The male of new species is most similar to M. calcarus by the segment 20 of right antennule with serrated spine, and the armature of P5: on right P5 (1) the presence of strong and stout coxal spine, (2) Exp-2 with similar shape and size, and (3) Exp-2 with strong and bent principal lateral spine; on left P5 (1) basis with long and narrow hyaline membrane on inner margin, and (2) Exp-2 with row of strong spinules on inner margin at distal half of segment. However, M. loeiensis sp. n. differs from M. calcarus by following morphological characters: (1) the new species with three chitinous processes on ventral surface of the right caudal ramus while M. calcarus with only two chitinous processes, (2) intercoxal plate of the new species with outgrowth process into two-spine like lobes at distal margin while M. calcarus without any outgrowth process, (3) basis of right P5 in the new species with inner hyaline membrane and with sub-globular chitinous process on caudal surface while M. calcarus without hyaline membrane, and with spur-like chitinous process, (4) the new species with long and slender end claw while it is typical short and robust in M. calcarus. Additional differences occur in female characteristics as follows: (1) the genital somite of M. loeiensis sp. n. with bulges on proximal, sub-middle, and middle region at right margin while M. calcarus is slightly convex on those region, (2) the right side of genital somite with spine located on outgrowth process in M. loeiensis sp. n. but it is directly inserted on somite in M. calcarus, (3) the left side of genital somite with slightly proximal dilated and spine inserted on small prominence while M. calcarus with obviously proximal rounded lobe and spine directly inserted on its segment.
Body length (Fig.
Mongolodiaptomus loeiensis sp. n. Female: A habitus, dorsal view B rostrum, frontal view C lateral wings on pediger 5 and genital somite, dorsal view D urosome, ventral view (black arrow points to smooth region of lateral seta) E genital somite, lateral view F lateral wing on pediger 5 and genital somite, lateral view G antennule. Scale bar 100 µm.
Caudal rami (Figs
Antennule (Fig.
Antenna (Fig.
Mandible (Fig.
Maxillule (Fig.
Maxilla (Fig.
Maxilliped (Fig.
P1–P4 (Figs
Coxa | Basis | Exopod | Endopod | |||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 1 | 2 | 3 | |||
P1 | 0-1 | 0-0 | I-1 | 0-1 | I-3-2 | 0-1 | 1-2-3 | ---- |
P2 | 0-1 | 0-0 | I-1 | I-1 | I-3-3 | 0-1 | 0-2 | 2-2-3 |
P3 | 0-1 | 0-0 | I-1 | I-1 | I-3-3 | 0-1 | 0-2 | 2-2-3 |
P4 | 0-1 | 1-0 | I-1 | I-1 | I-3-3 | 0-1 | 0-2 | 2-2-3 |
P5 (Figs
Additional ornamentation of P1–P5 as in Figs
Adult females with a single egg sac containing 8–10 eggs.
Body length (Fig.
Mongolodiaptomus loeiensis sp. n., SEM photographs. Female (A–C): A pediger 5 and genital somite, dorsal view B rostrum, frontal view C urosomite 2, anal somite and caudal rami, dorsal view; Male (D–G): D1 urosome in dorsal view D2 urosome in ventro-lateral view E anal somite and caudal rami, ventral view F the right antennule segments 8–18 (white arrows point to spines) G spinous process on right antennule segment 20.
Antennule (Figs
Left antennule, antenna, mouthparts, and P1–P4 similar to those in female.
P5 (Figs
Mongolodiaptomus loeiensis sp. n., SEM photographs. Male (A–E): A P5 in caudal view B coxa, basis, Exp-1 of the right P5 in caudal view C intercoxal plate, coxa, basis, Enp of the right P5 and coxa, basis, Exp and Enp of the left P5, caudal view DEnp and Exp of the right P5 in caudal view E P5 in frontal view; Female (F–I): F P5 in caudal view G basis, Exp-1 and Enp in caudal view (white arrow indicated the segmented point of Enp) H P5 in frontal view I the left P5 in frontal view.
The specific name loeiensis refers to the place “Loei” where the new species was first recognized. The name with the Latin suffix “-ensis” is an adjective for place.
Mongolodiaptomus loeiensis sp. n. was found in a temporary pond with a mean water temperature of 25.5 °C, conductivity 259 μS/cm, and pH 7.6. The new species was a single calanoid copepod occurring in type locality. However, it was found co-occurring with other microcrustaceans, i.e. Diaphanosoma excisum Sars, 1885, Thermocyclops decipiens (Kiefer, 1929), and Mesocyclops thermocyclopoides Harada, 1931. In over 3,000 samples collected from Thailand (59 samples from Loei Province provided by the first author and about 3,000 samples from other provinces by the second author and her colleagues), the new species can by considered as a rare species because it was present only in the type locality from Loei Province (
The occurrence of the new species is similar to that of Phyllodiaptomus thailandicus Sanoamuang & Teeramaethee, 2006 and Tropodiaptomus cf. ruttneri (Brehm, 1923), which have so far been known as rare species in Thailand and here each species was found in a single location or in about 2% of collected samples. P. thailandicus has hitherto been found in six provinces; Prachinburi, Chanthaburi, Sa Kaeo, Suphanburi, Kanchanaburi, and Chumphon (
The ranges of water variables for four other diaptomids species collected from Loei Province areas are as follows: Tropodiaptomus cf. ruttneri in water with temperature 26.9–30.1 °C, conductivity 81–100 μS/cm, and pH 7.1–7.3; P. thailandicus = 25.0–30.1 °C, 310–360 μS/cm, and pH 7.3–7.5; M. calcarus = 23.6–31.2 °C, 197–287 μS/cm, and pH 7.6–8.0; M. botulifer = 26.0–30.7 °C, 57–343 μS/cm, and pH 7.2–7.8; P. praedictus = 24.6–31.8 °C, 57–386 μS/cm, and pH 7.6–8.2; V. blachei = 26.0–31.4 °C, 92–527 μS/cm, and pH 7.1–8.1.
The genus Mongolodiaptomus currently contains 12 species, M. birulai, M. botulifer, M. calcarus, M. dumonti, M. formosanus, M. gladiolus, M. malaindosinensis, M. mephistopheles, M. pectinidactylus, M. rarus, M. uenoi, and M. loeiensis sp. n. (
The male P5 of M. loeiensis sp. n. can be distinguished from its congeners by the following characters: (1) two spine-like lobes on distal margin of the intercoxal plate in the new species versus a triangular lobe in M. birulai, M. botulifer, M. formosanus, M. pectinidactylus, and M. malaindosinensis and one spine-like lobe in M. uenoi and no outgrowth in M. dumonti, M. gladiolus, M. mephistopheles, and M. rarus; on the right P5, (2) the coxal spine of the new species stronger and more robust compared to those in other species (except M. uenoi), (3) basis of the new species with a hyaline membrane on inner margin, which is absent in M. gladiolus and M. dumonti, (4) chitinous prominence present on caudal surface on the basis of the new species while absent in M. birulai, M. formosanus, M. gladiolus, M. mephistopheles, M. malaindosinensis, and M. uenoi (M. botulifer has chitinous ridge), (5) Exp-1 of the new species with produced process at distal outer corner versus absent in M. birulai, M. dumonti, M. gladiolus, M. pectinidactylus and M. rarus, (6) Exp-2 of the new species with a bent principal lateral spine versus straight in M. birulai, M. gladiolus, M. dumonti, M. pectinidactylus and M. uenoi, (7) on left P5 basis of the new species presence of a hyaline membrane on inner margin versus absent in M. gladiolus and M. formosanus. In the female, the genital somite of M. loeiensis sp. n. has a large posterolaterally directed outgrowth on the right side which is absent in the others (except M. botulifer, M. gladiolus, M. malaindosinensis and M. rarus).
Males: | ||
1 | Spinous process on antepenultimate segment smooth | 2 |
– | Spinous process on antepenultimate segment serrate | 5 |
2 | P5 Enp 1-segmented | 3 |
– | P5 Enp 2-segmented | 4 |
3 | The basis of left P5 without hyaline membrane on inner margin | M. formosanus |
– | The basis of left P5 with hyaline membrane on inner margin | M. birulai |
4 | The basis of right P5 with triangular hyaline membrane on inner margin | M. malaindosinensis |
– | The basis of right P5 with spherical hyaline membrane on inner margin | M. botulifer |
5 | Intercoxal plate of P5 with outgrowth on distal margin | 6 |
– | Intercoxal plate of P5 without outgrowth on distal margin | 8 |
6 | The principal lateral spine on Exp-2 of right P5 straight | 7 |
– | The principal lateral spine on Exp-2 of right P5 bent | M. loeiensis sp. n. |
7 | Intercoxal plate of P5 with rounded lobe on distal margin | M. pectinidactylus |
– | Intercoxal plate of P5 with spine-like lobe on distal margin | M. uenoi |
8 | The basis of right P5 with hyaline membrane on inner margin | M. mephistopheles |
– | The basis of right P5 without hyaline membrane on inner margin | 9 |
9 | The basis of left P5 with hyaline membrane on inner margin | 10 |
– | The basis of left P5 without hyaline membrane on inner margin | 11 |
10 | The principal lateral spine on Exp-2 of right P5 straight | M. dumonti |
– | The principal lateral spine on Exp-2 of right P5 bent | M. calcarus |
11 | The basis of right P5 with chitinous spur on caudal surface | M. rarus |
– | The basis of right P5 without any process on caudal surface | M. gladiolus |
Females: | ||
1 | P5 Enp 1-segmented | 2 |
– | P5 Enp 2-segmented | 8 |
2 | Genital somite with postero-laterally directed outgrowth on right side | 3 |
– | Genital somite without postero-laterally directed outgrowth on right side | 6 |
3 | The left spine inserted on lobe-process of genital somite | M. gladiolus |
– | The left spine inserted directly on genital somite | 4 |
4 | Genital somite with posterolateral bulging | 5 |
– | Genital somite without posterolateral bulging | M. uenoi |
5 | P5 with long Enp, reaching beyond distal end of Exp-1 | M. malaindosinensis |
– | P5 with short Enp, not reaching distal end of Exp-1 (2/3 of Exp length) | M. botulifer |
6 | P5 Exp-3 absent | M. birulai |
– | P5 Exp-3 present | 7 |
7 | Genital somite with larger spine on left side compared to right side | M. mephistopheles |
– | Genital somite with similar sized spine on left and right side | M. formosanus |
8 | Genital somite with postero-laterally directed outgrowth on right side | 9 |
– | Genital somite without postero-laterally directed outgrowth on right side | 10 |
9 | Genital somite with hyaline membrane at mid-laterally on right side | M. rarus |
– | Genital somite without hyaline membrane at mid-laterally on right side | M. loeiensis sp. n. |
10 | The right spine inserted on small lobe of genital somite | M. pectinidactylus |
– | The right spine inserted directly on genital somite | 11 |
11 | Genital somite with larger spine on left side compared to right side | M. dumonti |
– | Genital somite with larger spine on right side compared to left side | M. calcarus |
We would like to thank the Higher Education Research Promotion and National Research University project of Thailand, the Office of the Higher Education Commission (Grant No. 2559A13462002) and the Thailand Research Fund (Grant No. MRG5980183) for financial support. We are grateful to Prof. Dr. Y. Ranga Reddy for providing some literature on diaptomids and critically reviewing the manuscript. We also thank Dr. D. Defaye for her careful editing of the manuscript.