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Research Article
A new large tellinid species of the genus Pharaonella from the Ryukyu Archipelago, Japan (Mollusca, Bivalvia)
expand article infoMakoto Kato, Luna Yamamori, Ryutaro Goto, Remi Tsubaki§, Ken Ohsuga|
‡ Kyoto University, Kyoto, Japan
§ Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan
| Unaffiliated, Kyoto, Japan
Open Access

Abstract

A new tellinid species, Pharaonella amanyu sp. n., is described from sand banks around Amami Islands, the Ryukyu Archipelago, in southern Japan. A molecular phylogenetic analysis suggests that this new species is closely related to P. sieboldii. This species has long siphons and lives buried deep in well-sorted white sand syntopically with Tonganaella tongana. These rare, large tellinid species are indicators of unspoiled tidal/subtidal sand flats, which should receive the highest priority conservation in the Ryukyu Archipelago.

Keywords

Amami Island, bivalve, coral reef ecosystem, sand flat, Tellinidae

Introduction

Coral reef ecosystems often contain sand flats in which the sediments are mainly composed of coral sand. Even though the biodiversity of tropical coral reefs is both extremely high and the species themselves sometimes endangered (Robert et al. 2002), the biodiversity of the sand flats within coral reef ecosystems has not yet been fully explored. Edateku Island is an uninhabited island facing Amami-Oshima Island across a strait located in a coral reef ecosystem of the northern Ryukyu Archipelago. The east coast of Edateku Island harbours an unspoiled sand flat with well-sorted sand grains inhabited by gobioid sand darts (Tsubaki and Kato 2009). To assess the conservation value of this sand flat, we have conducted an extensive survey of the molluscan biodiversity.

The sand flat is characterised by a diverse fauna of tellinid bivalves, which are surface deposit feeders with colourful thin shells and long extensible siphons. The genus Pharaonella (Lamy, 1918) and the recently described Tonganaella Huber, Langleit & Kreipl, 2015 are both characterised by large narrow shells with rostration posteriorly, and both occur in tidal and subtidal sandy substrata, where they are buried deeply and extend their long siphons up to the surface of the sediment. In these genera, four species are presently known from Japan: T. perna (Spengler, 1798); T. tongana (Quoy & Gaimard, 1835); P. aurea (Perry, 1811); P. sieboldii (Deshayes, 1855). In this survey on Edateku Island, several shells of an unidentified tellinid species belonging to one of these genera were collected. The shells resemble the Pharaonella sp. reported from Amami Islands by Nawa (2008) and interpreted as a putative new species. Taxonomic study of this species has been impeded by its rarity and the lack of live specimens for anatomical examination. At last in 2013, a live specimen of this species was found. Morphological and anatomical observations suggest that it belongs to the genus Pharaonella. To clarify the specific status and phylogenetic position of the new species, we made molecular phylogenetic analyses of Pharaonella species and related taxa in Tellinidae.

In this paper, this bivalve is described as a new species, its special habitat reported, and the conservational priority of the unspoiled intertidal/subtidal sand banks bounding a strait between coral reefs is emphasised.

Materials and methods

An intertidal/subtidal sand flat is present along the eastern coast of Edateku Island in the northern Ryukyu Archipelago, Kagoshima Prefecture, Japan (28°17'26.08"N, 129°13'9.09"E) (Fig. 1). The sediment of the sand flat is well-sorted white sand derived from comminuted corals (Fig. 2A), and the sand flat is inhabited by infaunal organisms, the most characteristic of which are the sea cucumbers Bohadschia bivittata (Fig. 2B) and Holothuria leucospilota (Holothuriidae), the spatangoid sea urchin Metalia sternalis (Brissidae), the lancelet Branchiostoma belcheri (Branchiostomidae, Fig. 2E) and the fishes Kraemeria cunicularia (Kraemeriidae) and Trichonotus elegans (Trichonotidae) (Fig. 2F).

Figure 1. 

The locality of Pharaonella amanyu sp. n. in the Amami Islands, Ryukyu Archipelago. The sand flat constituting the type locality of this new species is shown by an arrow.

To characterise the molluscan biodiversity of the sand flat, we sampled the molluscs, particularly the tellinid bivalves, by digging the sand with shovels at low tide during the spring tides in May or June each year from 2005 to 2016. Three Tonganaella/Pharaonella species were found in these samples: T. perna (Spengler, 1798), T. tongana (Quoy & Gaimard, 1835) (Fig. 2D), plus an unidentified species (Fig. 2C). The third species was very rare, and live specimens were not collected until 26 May 2013. The body of this species was preserved in 99% ethanol, and was utilised for malacological and genetic analyses.

Figure 2. 

A panorama of the sand flat of Edateku Island at spring low tide (A) and the sand-dwelling organisms therein (B–F): B Bohadschia bivittata C Pharaonella amanyu D Tonganaella tongana E Branchiostoma belcheri F Trichonotus elegans.

Sequence data were obtained for the mitochondrial cytochrome c oxidase subunit I (COI) gene, nuclear 28S ribosomal RNA (28S rRNA) gene, and nuclear histone 3 (H3) gene of five tellinid species: two Pharaonella, two Tonganaella and one Tellinides species (Table 1). Total DNA was isolated from adductor muscle tissue following a previously described method (Sokolov 2000). We sequenced fragments of the mitochondrial COI gene, 28S rRNA gene and H3 gene. Polymerase chain reaction (PCR) was used to amplify ~700 bp of COI using the universal primers LCO1490/HCO2198 (Folmer et al. 1994), ~1000bp of 28S rRNA gene using the primers D1 (Colgan et al. 2003) and D3 (Vonnemann et al. 2005) and ~350bp of H3 using the primers H3F/H3R (Colgan et al. 1998). Sequencing reactions were performed using the PCR primers for COI gene and H3 gene, and PCR primers and additional sequencing primers, D2F (Vonnemann et al. 2005)/C2R (Dayrat et al. 2001), for 28S rRNA gene, with a BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA) on an ABI 3130 sequencer (Applied Biosystems). The obtained sequences were deposited in the DDBJ/EMBL/GenBank databases with accession numbers (Table 1).

A list of bivalve species analyzed for genetic sequences of three genes (COI, 28S rRNA and H3).

Family Species Specimen GenBank # Locality
Catalogue # COI 28S rRNA H3
Tellinidae Macoma balthica GenBank KC429141 KC429501 KC429224
Megangulus zyonoensis GenBank JX503037 AB746875 NA
Moerella iridescens GenBank JN398362 AB746876 NA
Pharaonella amanyu sp. n. NSMT-Mo 78982 (holotype) LC311753 LC311747 LC311757 Edateku Is., Kagoshima, Japan
Pharaonella sieboldii KUZ-Z1880 NA NA LC311758 Notojima, Ishikawa, Japan
Tellinella crucigera GenBank KC706878 NA NA
Tellinella cumingii KUZ-Z1881 NA LC311748 LC311759 Edateku Is., Kagoshima, Japan
Tellinella virgata GenBank/ KUZ-Z1882 AB741079 LC311749 LC311760 Yohena, Okinawa, Japan
Scissula similis GenBank KC429142 KC429502 KC429225
Tellinides ovalis KUZ-Z1883 LC311754 LC311750 LC311761 Yohena, Okinawa, Japan
Tonganaella perna KUZ-Z1884 LC311755 LC311751 LC311762 Yohena, Okinawa, Japan
Tonganaella tongana KUZ-Z1885 LC311756 LC311752 LC311763 Edateku Is., Kagoshima, Japan
Semelidae Abra alba GenBank KT307619 KF741656 KC429228

The sequences were aligned using Muscle (Edgar 2004) as implemented in Seaview software (Galltier et al. 1996; Gouy et al. 2010) using the default settings. Gblocks v0.91b (Castresana 2000; Talavera and Castresana 2007) was employed to eliminate the ambiguously aligned regions of the 28S sequence. The sequence length of the 28S gene before and after Gblocks treatment was 1046 and 1005, respectively. Alignments of the COI gene and H3 gene did not contain insertions or gaps and were therefore unambiguous.

Bayesian and maximum likelihood (ML) phylogenetic analyses were performed based on the combined data set (28S + COI + H3) using MrBayes 3.1.2 (Ronquist and Huelsenbeck 2003) and RAxML version 7.4.2 (Stamatakis 2006) implemented in raxmlGUI ver.1.31 (Silvestro and Michalak 2012). We selected the model GTRGAMMA for RAxML analysis and used the software Kakusan4 (Tanabe 2011) to choose the appropriate models for the MrBayes analysis. The models selected for the MrBayes analysis were GTR_GAMMA for 28S gene, HYK85_GAMMA, GTR_GAMMA and F81_GAMMA for each codon of COI, and GTR_GAMMA for the first codon of H3 gene and J69_Homogenoeous for the second and third codon of H3 gene.

Systematic account

Superfamily Tellinoidea Blainville, 1814

Family Tellinidae Blainville, 1814

Pharaonella Lamy, 1918

Pharaonella amanyu Kato & Ohsuga, sp. n.

Figs 2C, 3A–G, 4A, 5A

Description

Shell. Shell elongate, narrow, subequilateral; inequivalve; anterior section longer than posterior section (Fig. 3A–C); posterior end rostrate, slightly twisted to right (Fig. 3E); weakly gaping posteriorly; left valve glossy, smooth, with faint radials; right valve weakly commarginal ridged on rostrum; exterior color orange to pink, sometimes with fine pale rays emanating from umbo (although the colour of the shell tends to fade once the animal has died); periostracum thin, shiny, slightly iridescent; interior orange to pink with yellow tint in central section; umbones small, posteriorly displaced and touching each other; hinge ligament external, short, and situated in ligamental groove; right valve hinge with 2 elongate lateral teeth, 1 oblique anterior cardinal tooth plus 1 bifid posterior cardinal tooth (Fig. 3F); left valve hinge with 2 distal elongate laterals, 1 trigonal anterior and 1 laminate posterior cardinal tooth (Fig. 3G); posterior edentulous space expanded in both valves; pallial sinus reaching horizontal midline in height of the shell and extending beyond vertical midline in length, confluent with pallial line at middle of shell (Fig. 3D); adductor muscle scars subequal and suborbicular.

Figure 3. 

Right (A, C, F, H, K, M, N), left (B, D, G, I), and paired (E, J, L) valves of tellinid species: A–G Pharaonella amanyu H–J Tonganaella tongana K–L T. perna M P. aurea N P. sieboldii. Scale bar 10 mm.

Anatomy. Mantle and foot orange, thus similar to colour of shell (Fig. 4A), contrasting with creamy white of those of Tonganaella tongana (Fig. 4B). Excurrent and incurrent siphons long, similar to each other in length. Siphons able to be extended further than shell length when alive.

Figure 4. 

Anatomy of live specimens of Pharaonella amanyu (A) and Tonganaella tongana (B). Abbreviations: aa, anterior adductor muscle; de, demibranch; es, excurrent siphon; ft, foot; go, gonad; is, incurrent siphon; pa, posterior adductor muscle; pl, palp. Scale bar 10 mm.

Labial palps well developed in comparison with demibranchs. Outer and inner hemipalps elongate-triangular (Fig. 5A), posterior extension of hemipalp weaker than those of T. tongana (Fig. 5B) and P. sieboldii (Fig. 5C). Inner surfaces of hemipalps have palp folds, which originating from hemipalp intersection spreading toward palp dorsal edge; folds becoming relatively wider distally; distal edges of folds of outer hemipalp form swellings (Fig. 5A).

Figure 5. 

Right hemipalps with outer one reflected: A Pharaonella amanyu B Tonganaella tongana C P. sieboldii. Scale bar 5 mm.

Type material

Holotype: NSMT-Mo 78982, paired valves, length 69 mm, height 32 mm (Figs 3A, B, E, 4A), collected alive by M. Kato on 26 May 2013. Paratype: NSMT-Mo 78983, right valve, length 80 mm, height 36 mm (Fig. 3C, G), collected by K. Ohsuga on 28 April 2006; KUZ-Z1878, left valve, length 73 mm, height 33 mm (Fig. 3D, F), same data as the former paratype; KUZ-Z1879, right valve, length 77 mm, height 34 mm, collected by K. Ohsuga at type locality13 July 1995.

Type locality

Edateku Island, Uken, Kagoshima Prefecture, Japan (28°17'26.08"N, 129°13'9.09"E); in sand of subtidal sand bank.

Distribution

In addition to the type locality, the species has also been recorded at Kasari Bay on Amami-Oshima Island, but only by empty shells.

Etymology

The epithet amanyu alludes to the mythical archaic peaceful era of Amami Islands, symbolising the undisturbed coastal ecosystem harbouring this bivalve species. It is used as a noun in apposition.

Japanese name

Aman’yu-beni-gai.

Habitat

The bivalve was found deeply buried (about 15 cm) in subtidal sandy bottom.

Molecular and phylogenetic analyses

For molecular phylogenetic analysis, COI, 28S rRNA, and H3 sequence data were compared for several Tonganaella and Pharaonella species (Table 1). To examine the phylogenetic relationships among these species, outgroup species belonging to the Tellinidae and Semelidae were selected from GenBank. The phylogenetic analysis based on the combined data set (COI+28S+H3) (Fig. 6) suggested that Pharaonella and two Tonganaella species formed a monophyletic clade whereas the Tonganaella species were not monophyletic. The tree also suggests that P. amanyu is a distinct species most closely related to P. sieboldii. The single morphological characteristic that is unique to Pharaonella is the set of commarginal grooves on the right shell valve (Huber et al. 2015). In P. amanyu, this sculptural element is present only at the beak.

Figure 6. 

Bayesian tree of Pharaonella and Tonganaella species based on sequences of COI, 28S rRNA, and H3 genes. The numbers above the branches are Bayesian posterior probabilities followed by maximum likelihood bootstrap support values.

Discussion

Both morphological and molecular phylogenetic analyses suggest that the new species should be assigned to the genus Pharaonella. The morphological characteristic unique to Pharaonella is the commarginally grooved sculpture of right shell valves (Huber et al. 2015), a feature exhibited by the new species. In P. amanyu sp. n. this sculpture is confined to the beak of right shell valve. The new species is distinguished from the other Japanese Pharaonella species (P. aurea and P. sieboldii), by their much narrower shells with longer rostrations (Table 2).

Nawa (2008) illustrated a Pharaonella sp. specimen collected in Amami Island, and Huber et al. (2015) suggested that this specimen might be Pharaonella dialeuca (Deshayes, 1855), a species described from West Malaysia. Pharaonella dialeuca differs from P. amanyu as follows: (1) the shell of P. dialeuca is narrower than P. amanyu , (2) P. dialeuca has two white rays on shells but P. amanyu has numerous thin pale rays, (3) the commarginally grooved sculpture of right shells occurs on the posterior half in P. dialeuca but confined in beak in P. amanyu (Table 2). In the Philippines, a somewhat similar orange colored tellinid is illustrated as “Tellina (Pharaonella) perna” in Springsteen and Leobrera (1986). The specimen has broad shells (the length/height ratio being 1.94) and so it differs from the narrower T. perna, P. dialeuca, and P. amanyu (Table 2).

Although P. amanyu resembles Tonganaella species, it can be separated from T. perna by the orange colour of shells (white, yellowish or pinkish in T. perna) and the long anterior part of the shell (the posterior part is longer than the anterior part in T. perna), and from T. tongana by the orange colour of the shells and the absence of pink rays at the umbones (Table 2). Our molecular phylogenetic analyses also suggest that the genus Tonganaella is not monophyletic and should be synonymised under Pharaonella, but this synonymy is not undertaken here because further morphological studies would be necessary.

Morphological characters of Tonganaella and Pharaonella species.

Bivalve species Shell narrowness (shell length/shell height) Shell shape Shell color Sculpture of right valve Pallial sinus Distribution
Pharaonella aurea narrowest (2.8–3.0) anterior part longer than posterior part bright red or rarely yellow commarginally ridged deep from the Ryukyu Archipelago to Northern Australia
P. dialeuca narrow (2.4) anterior part longer than posterior part yellowish orange with two white umbonal divergent rays commarginally ridged in posterior half unknown Philippine Islands
P. sieboldii very narrow (2.3–2.4) anterior part longer than posterior part pink commarginally ridged deep around the Japanese Archipelago
P. amanyu narrow (2.1–2.2) anterior part longer than posterior part orange with many pale umbonal rays commarginally ridged only in posterior beak moderate around Amami Islands
Tonganaella perna narrow (2.1–2.2) anterior part shorter than posterior part white or cream, rarely pink smooth moderate from the Ryukyu Archipelago to Northern Australia
T. tongana least narrow (1.9–2.0) anterior part as long as posterior part white, sometimes reddish or yellowish, with pink rays only around unbones smooth moderate from the Ryukyu Archipelago to Northern Australia

It is remarkable that such a large bivalve living near the tidal zone has not been described until now. Among marine bivalves, there are several local endemic species confined to coastal ecosystems in the Ryukyu Archipelago: Gafrarium yukitai Habe, 1977 (Veneridae), Peregrinamor gastrochaenans Kato & Itani, 2000 (Galeommatidae), and Merisca monomera Habe, 1961, Semelangulus lacrimadugongi Kato & Ohsuga, 2007 (Tellinidae). The habitats of these endemic species are sandy or muddy tidal flats around estuaries, and the new species may provide another example of these endemic species confined to the Ryukyu Archipelago.

The type locality of the new species is the sand flat at Edateku Island, which is formed by clean white coarse sand lying along a narrow strait and harbours diverse characteristic sand-burrowing organisms such as sea cucumbers, spatangoid sea urchins, lancelets, fishes and molluscs such as terebrid, olivid, and naticid snails, and tellinids. It is noteworthy that one Pharaonella and two Tonganaella species should be recorded from the sand flat, because many of the tellinid bivalve species are now threatened in the Ryukyu Archipelago (Nawa 2008; Okinawa Prefecture 2017). Although tellinid bivalves are often abundant in seagrass beds within coral reef ecosystems, only small stands of seagrass exist at the Edateku sand flat. In addition to the new species, Tonganaella tongana is also a rare species that is now found at only a few sites such as this sand flat and Oura Bay, where reclamation is planned (Diving Team Snack Snufkin 2015). The discovery of the new species, P. amanyu, reinforces the significance for conservation value of these sandy habitats in the Ryukyu Archipelago.

Sandy intertidal/subtidal flats and sandbanks form in inland seas along straits where tidal currents are strong (Takasugi et al. 1994). In the Seto Inland Sea of Japan, many sand flats and sand banks were found, harbouring characteristic sand-dwelling organisms such as lancelets (Branchiostoma belcheri) and sand lances (Ammodytes personatus). Although these sandy habitats are fundamental to ensuring the productivity of coastal waters and to sustaining the fishery resources of inland seas, most such habitats disappeared between 1970–2000 in Japan because of sand mining (Yoshino et al. 2006). In the Amami Islands also, ongoing sand mining is a serious problem for the conservation of coastal ecosystems. To conserve the biodiversity of the sand flat ecosystem of Edateku Island, sand mining must be prohibited in the strait between Edateku Island and Amami-Oshima Islands.

Acknowledgments

The authors thank the Fisherman’s Union of Uken Village for permitting and helping us to study the marine fauna at the sand flat of Edateku Is., and Dr Richard C. Willan and Dr Lisa Kirkendale for their helpful comments on our manuscript. This research was supported in part by a Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (A) 2010–2014 (22247003, awarded to M. Kato).

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