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Research Article
Honduranura centraliamericana gen. n. et sp. n. from Central America (Collembola, Neanuridae, Neanurinae)
expand article infoJosé G. Palacios-Vargas
‡ Universidad Nacional Autónoma de México, México, Mexico
Open Access

Abstract

Honduranura gen. n. and the type species H. centraliamericana sp. n. are described and illustrated. The new genus shows the characters of Sensillanurini Cassagnau, 1983 tribe and is distinguished by the fusion of cephalic and abdominal tubercles: clypeal with antennofrontal and dorsointernal with dorsoexternal on head; presence of fused tubercles on each side of abdominal segment V. Most of the tubercles bear strong subcuticular reticulation. A key for the genera of the tribe Sensillanurini is provided.

Keywords

Honduras, Costa Rica, morphology, Sensillanurini, taxonomy

Introduction

To date, the tribe Sensillanurini contains only four genera: Americanura Cassagnau, 1983, with a wide distribution in North America, México, Central America, and the north of South America; Palmanura Cassagnau, 1983, with a Neotropical distribution from Central México to north of South America; Sensillanura Deharveng, 1981, with Holarctic and Nearctic distribution and Tabasconura, Palacios-Vargas & Catalán, 2015, endemic of Mexico. The tribe Sensillanurini has a high diversity of species in the Neotropical Region (46 out of 49 named species), and is characterized by hypertrophy of the sensillum S7 on antennal segment IV (Deharveng 1981; Palacios-Vargas et al. 2009), development of cuticular tubercles and reduction of the chaetotaxy. The new genus seems to be wide distributed in Central America (from Honduras to Costa Rica). It is distinguished by the fusion of some tubercles and the elongation of the dorsolateral and lateral ones on tergites.

Materials and methods

Samples of leaf litter were collected at Camayagua, Honduras and processed by Berlese-Tullgren funnels. Specimens of Neanuridae were kept in 75 % alcohol and sent to the author. Members of the new genus were cleared and mounted in Hoyer’s solution under slides. Observations and measurements were made using a Carl Zeiss Axiostar Plus phase contrast microscope with an adapted drawing tube. Dorsal chaetotaxy follows Deharveng and Weiner (1984) modified by Palacios-Vargas and Catalán (2010) and ventral chaetotaxy follows Smolis (2008) and Smolis and Deharveng (2006).

Abbreviations

Abd abdominal segment

Af cephalic antenno-frontal tubercle

asl above sea level

Ant antennal segment

Cl clypeal tubercle

Cx coxa

Di dorso-internal tubercle

De dorso-external tubercle

DL dorso-lateral tubercle

Fe femur

Fu furcal vestige

L lateral tubercle

L’ ordinary lateral seta on Abd. V

M macrosetae

me mesosetae

mi microseta

m’ ventral microsensillum of Ant III

Oc ocular tubercle

Ocm ocular median seta

Ocp ocular posterior seta

or subapical organ of Ant. IV

S cylindrical sensillum on Ant IV

Scx2 subcoxa 2

sgd dorsal guard sensillum

sgv ventral guard sensillum

So sub-ocular tubercle

ss sensorial setae on body

T tibiotarsus

Th. thoracic segment

Tr trochanter

VT ventral tube

Ve ventroexternal

Vel ventroexternolateral

Vec ventroexternocentral

Vei ventroexternointernal

Vi ventrointernal

Vl ventrolateral.

Results

Taxonomy

Honduranura gen. n.

Type species

Honduranura centraliamericana sp. n.

Diagnosis

Neanuridae with aspect of a yellow Neanura. 2+2 slightly pigmented big eyes. Body color yellow or orange when alive, without blue pigment, almost white in alcohol (Fig. 1). Mouthparts reduced, maxillae styliform. Sensilla S7 on Ant. IV hypertrophied, at least twice thicker than others. Clypeal and Antennofrontal tubercles fused altogether, cephalic setae A, B, E, F and G present (O, C and D absent) (Fig. 2). Two ocular setae: Ocm and Ocp. Posterior cephalic tubercles Di and De fused at each side, Di1, Di2, De1 and De2, not in crossed pattern. Dorsolateral tubercle (DL) separate, with two me and two M setae. Lateral tubercle with two M and two me, subocular tubercle with 5 setae. Thorax I with two M setae on De and one M seta on DL tubercle, without setae or tubercle on Di position. Di tubercle of Th. II and III with three setae, one M and two mi; Di tubercle on Abd. I–III with two setae, one M and one me. De tubercle with two setae one M and one me, plus ss from Th. II to Abd. III. Tubercle Di on Abd. IV with two setae M and mi, other tubercles with lateroexternal migration. Abd. V with tubercles Di, De and DL fused, with three setae and one sensorial seta ss. Four (2+2) macrosetae between the sensorial setae on Abd. IV, two (1+1) setae between sensorial setae of Abd. V. Head and body tubercles with strong subcuticular reticulation. Distal part of abdomen strongly bilobed.

Figures 1–3. 

Honduranura centraliamericana sp. n. 1 habitus on slide 2 antennofrontal + clypeal tubercle 3 dorsal chaetotaxy of head and thoracic segments I–II.

Remarks

This is the only genus in the Sensillanurini tribe with clypeal and antennofrontal tubercles fused on head, dorso-internal and dorso-external tubercles fused on each side of the head, and tubercles of abdomen V fused on each side of the body. In addition, all tubercles exhibit a strong subcuticular reticulation, and dorsolateral and lateral tubercles slightly elongated.

Etymology

The name of genus is after the country where the author had seen specimens for the first time and it is the type locality of the type species. Gender of genus is feminine.

Key for the genera of Sensillanurini

1 Presence of Di tubercle and one seta on Th. I Sensillanura
Lacking Di tubercle and seta on Th. I 2
2 Cephalic tubercles Di and De; Cl and Ant fused Honduranura gen. n.
Cephalic tubercles clearly isolate 3
3 Dorsal tubercles developed and “finger-like”; S2 hypertrophied and thickened similar to S7 on Ant. IV Tabasconura
Dorsal tubercles not elongated; S2 not hypertrophied, thin and short, similar to others except S7 4
4 Cephalic tubercle De with 3‒1 setae, two or one setae on tubercle Di of Abd. IV and V; most dorsal macrosetae smooth or barbulate Americanura
Cephalic tubercle De always with 1 seta; only one seta on tubercle Di of Abd. IV and V; most dorsal macrosetae palmate with serrate margins Palmanura

Honduranura centraliamericana sp. n.

Figs 1–3, 4–7, 8–12, Tables 1, 2

Type material

Holotype: adult female; Paratypes: three adult females, one adult male and one juvenile. All the type material kept at author’s institution.

Type locality

Central America: Honduras: Camayagua (14°48'39"N; 87°53'22"W). 2140 m asl. FS2A LLAMA # Wa-C03-2-all, cloud forest, samples of leaf litter. 05.v.2010, F. Soto-Adames leg.

Other material

Central America: Costa Rica: Sierra de Talamanca. Parque Nacional Tapantí (9°46'14"N; 83°47'59"W). 1200 m asl, tropical rain forest, ex rotting trunk. 19.vii.2010, J. G. Palacios-Vargas col. One female and one juvenile.

Description

Length of holotype 2.5 mm; length range: 2.2–2.8 mm (n = 5). Color yellowish. Granulations strong, approximately 1/4 diameter of one eye. Tubercles well developed mainly on lateral and posterior part of body (Fig. 1), with strong subcuticular reticulation. Head with clypeal and antennofrontal tubercles fused with setae A, B, E, F, G present (O, C and D absent) (Fig. 2); posterior cephalic tubercles dorsointernal and dorsoexternal fused (Fig. 3). On Abd. V there is only one tubercle on each side (Fig. 4). Two kinds of dorsal body setae, macrosetae (M) 46 µm (38–60 µm) with blunt apex, mesosetae (me) with blunt apex, both slightly serrate in both sides, besides sensorial setae (ss) (30 µm). All ventral setae are smooth and acuminate; some are macrosetae and most are mesosetae.

Figures 4–7. 

Honduranura centraliamericana sp. n. 4 dorsal chaetotaxy of abdominals segments II–VI 5 dorsal antennal segments III and IV 6 chaetotaxy of pre-labrum/labrum 7 chaetotaxy of labium.

Ant. I with 9 setae, 4 dorsal slightly barbulate macrosetae on a surface with subcuticular reticulation. Ant. II with 11 setae, one of them slightly serrate. Ant. III sensorial organ with two globular sensilla in a cuticular fold, and two guard sensilla; S.g.d slightly curved, one microsensillum ventro-external. Ant. IV with hypertrofied sensilla S7; S2 like other sensilla. One clear subapical organite. Apical bulb of Ant. IV trilobed (Fig. 5).

Labrum with 4 short prelabral setae, two short basal setae and two long apical setae (Fig. 6). Labium without tuberculate seta L, organite “x” or seta A (as cited by Deharveng, 1983 for the subfamily) Seta D short; seta F much longer than E and G (Fig. 7). Eyes 2+2, large, with dark pigment. Mandibles with three teeth. Maxillae styliform. Head with clypeal and antennofrontal tubercles fused, ocular seta Ocm and Ocp in one independent tubercle, Di and De tubercles fused, DL and L tubercles independent and well developed. Head chaetotaxy in figure 3 and in Table 1. Three pairs of postlabial setae, the second one much larger and thicker than others (Fig. 7).

Legs chaetotaxy from coxae to tibiotarsi (I, II and III), respectively, as 3,7,7; 5,5,5; 10,10,10; 18, 18, and 17 setae, without capitate tenent hairs, but with setae B4 and B5 long and acuminate (Fig. 8). One ventral seta of trochanter is small and very thin. Each femur with one long ventral seta. Ungues with strong granulation but without tooth. Thoracic and abdominal chaetotaxy in Figs 3 and 4. Body chaetotaxy by half tergite is shown in Table 2.

Head chaetotaxy of Honduranura centraliamericana sp. n.

Head setae group Tubercles Number of setae Kind of setae Setae
Cl+Af 1 5 1M, 4me A, B, E, F, G
Oc 1 2 2M Ocm, Ocp
Di + De 1 4 2M, 2me Di1, Di2, De1, De2
DL 1 4 3M, 1 me
L 1 4 1M, 3 me
So 5 Mi
Total number 5 24

Thorax and abdomen chaetotaxy of Honduranura centraliamericana sp. n. by half tergite.

Thorax & Abdomen Dorsal Legs
Di De DL L Scx2 Cx Tr Fe T
Th. I 2M M 0 3 6 13 19
Th. II M, 2m M, me+ss M, 2me +ss M 2 7 6 12 19
Th. III M, 2m M, me+ss M, 2me +ss M 2 8 6 11 18
Abdomen Ventral
Abd. I M, me M, me+ss 1M, 1me 2M, 2me VT: 4
Abd. II M, me M, me+ss 1M, 1me 2M, 2me Ve: 6(5) Vel 0
Abd. III M, me M, me+ss 1M, 1me 2M, 2me Vel: 6(7) Fu: 4me 4mi
Abd. IV M, me (2M, me +ss) M, 5me Vel: 9 Vec: 2 Vei: 1 VI: 3
Abd. V (M + ss, 2M) M, me M, me Ag: 6 VI: 2 L: 1
Abd. VI 7– Ve:12 An: 2mi
Figures 8–12. 

Honduranura centraliamericana sp. n. 8 lateral view of tibiotarsus III chaetotaxy 9 furcular vestige (midventral region of Abd. III) 10 ventral chaetotaxy of Abd. II–VI 11 female genital plate 12 male genital plate.

Ventral tube with 4 + 4 setae, the two distal setae subequal in size, basal setae are different, one is larger. Furcal vestige with four mesosetae and four apical microsetae in the apex of a small tubercle (Figs 9, 10). Female genital plate with 6 + 6 pregenital, 28 circumgenital and two eugenital setae (Fig. 11), genital plate of the only male studied with 6 + 6 pregenital, 16 circumgenital and 2 + 2 eugenital (Fig. 12), but it should be 6 + 6, 22, and 4 + 4 respectively. Each lateral anal valve with subcuticular reticulation, 11 setae and 2 microsetae. Ventro-internal tubercle of Abd. V well-developed and with strong subcuticular reticulation, one macroseta and three mesosetae.

Etymology

The new species is named H. centraliamericana sp. n. for its distribution in Central America (Honduras and Costa Rica), but it might be even more widely distributed, as the two localities are approximately 800 km from each other.

Discussion

This species has the unique characters of this new genus: the fusion of clypeal and antennofrontal tubercles and of dorsointernal and dorsoexternal tubercles on head. Additionally, the presence of only one tubercle on each side of the abdominal segment V is unique among Sensillanurini. The new species has more abundant head chaetotaxy than members of Americanura and Palmanura, including the antennofrontal, dorsolateral and lateral cephalic tubercles, and Th. I which has no Di seta, against one in all species of the genus Sensillanura (Palacios-Vargas and Catalán 2010). The presence of nine setae on Ant. I have been cited in other member of the Neanurinae (Deharveng 1981), here, there are five dorsal slightly barbulate macrosetae on a surface with subcuticular reticulation like in the Neanura, Monobella and Neanurella species which exhibit this character, and which belong to different evolutionary lineages; the 6 + 6 pregenital setae is also a character unique in the tribe. The furcal vestige of the new species is like that of Sensillanura, but more developed, as a small tubercle similar to that of Morulina species, with mesosetae and microsetae.

Variation: The ag setae in females varies from 5-6 pairs, and circumgenital ones from 15 to 28 setae. One teratologic specimen lacks left tubercle of abdominal segment VI. Some of the mesosetae on Di tubercle of Th. II and III are very thin and smooth and can be overlooked. The juvenile paratype has ten setae on anal valve instead of eleven. The specimens from Costa Rica have the dorsal macrosetae and mesosetae acuminate.

Acknowledgments

Blanca E. Mejía Recamier (Fac. Ciencias, UNAM) prepared the microscopic slides for this study. Felipe Soto-Adames donated the specimens from Honduras. Fernando Villagomez (Fac. Ciencias, UNAM) made some of the drawings. Ana Isabel Bieler took the pictures of the habitus and details. Diego Fernández helped in Costa Rica during field work. Final plates were prepared by María Martínez. Adrian Smolis and Louis Deharveng gave criticism and important and suggestions.

References

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