Research Article |
Corresponding author: Zhe Zhao ( zhaozhe@ioz.ac.cn ) Academic editor: Yuri Marusik
© 2017 Xiaoqing Zhang, Zhe Zhao.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang X, Zhao Z (2017) A new species of Longicoelotes (Araneae, Agelenidae) from China, with the first description of the male of L. kulianganus (Chamberlin, 1924). ZooKeys 686: 137-147. https://doi.org/10.3897/zookeys.686.11711
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A new Longicoeletes species is described from Jiangxi Province, China: L. geei sp. n. (♂♀). In addition, the male of L. kulianganus (Chamberlin, 1924) is described for the first time. DNA barcodes of the two species are documented for future use and as proof of molecular differences between these species.
East Asia, description, Coelotinae , taxonomy
The Longicoelotes was described by
Specimens were examined and measured with a Leica M205C stereomicroscope. Images were captured with an Olympus C7070 wide zoom digital camera mounted on an Olympus SZX12 dissecting microscope. Epigynes and male palps were examined after dissection. Epigynes were cleared by boiling in 10% KOH solution before taking photos. All measurements are given in millimeters. Leg measurements are given as: total length (femur, patella + tibia, metatarsus, tarsus). Only structures (palp and legs) of the left side of the body are described and measured.
Terminology used for parts of copulatory organs in the text and figures follows
DNA barcodes were obtained for future use: a partial fragment of the mitochondrial gene cytochrome oxidase subunit I (COI) was amplified and sequenced for two species using Primers LCO1490-oono (5’-CWACAAAYCATARRGATATTGG-3’) (
Species | GenBank accession number | Sequence length | Collection localities |
---|---|---|---|
L. geei sp. n. | MF347606 | 543bp | China: Jiangxi: Wuyuan |
L. kulianganus | MF347607 | 630bp | China: Fujian: Fuzhou: Guling |
All specimens (including molecular vouchers) are deposited in the Institute of Zoology, Chinese Academy of Sciences in Beijing (IZCAS).
Longicoelotes Wang, 2002: 109. Type species Longicoelotes karschi Wang, 2002 from Jiangsu Province, China.
Four species of Longicoelotes in total: they are L. geei sp. n. (♂♀), L. karschi (♂♀), L. kulianganus (♂♀) from China, and L. senkakuensis (Shimojana, 2000) (♀) from Ryukyu Islands.
Longicolotes karschi is very similar to L. kulianganus and could be its junior synonym.
Holotype ♂: China: Jiangxi Province: Wuyuan County, Lianhua Cave, N29°29'02", E117°36'53", 350 m, 4.XII.2016, X. Zhang; Paratypes: 3♀ 2♂, same data as holotype; 3♀, the same locality, N29°29'02", E117°36'53", 352 m, 20.V.2013, Y. Luo and J. Liu.
The species is named after Mr. Nathaniel Gist Gee. Mr. Gee was an American biologist who lived in China about 35 years. He contributed greatly to the development of biological research and education in China, including the establishment of the first biology department in the history of university education in China (
The male can be distinguished from all other Longicoelotes species by its long cymbial furrow, about 1/3 length of cymbium (vs 1/4 or 1/5 in other species) (Figs
Palp of Longicoelotes geei sp. n., holotype male. A Prolateral view B Ventral view C Retrolateral view. CAT = anterior tip of conductor, CF = cymbial furrow, CO = conductor, CPT = posterior tip of conductor, E = embolus, EB = embolic base, MA = median apophysis, PA = patellar apophysis, ST = subtegulum, T = tegulum, TS = tegular sclerite, VTA = ventral tibial apophysis. Scale bar: equal for A, B, C.
Male (holotype): Total length 6.00. Carapace 2.50 long, 2.00 wide. Abdomen 3.50 long, 1.75 wide. Eye sizes and interdistances: AME 0.15, ALE 0.13, PME 0.15, PLE 0.10; AME-AME 0.05, AME-ALE 0.04, PME-PME 0.05, PME-PLE 0.06. Leg measurements: I: 12.45 (3.30, 4.00, 3.00, 2.15); II: 11.30 (3.25, 3.30, 2.75, 2.00); III: 10.65 (3.00, 3.25, 2.75, 1.65); IV: 14.65 (4.00, 4.25, 4.15, 2.25). Carapace greenish, with black lateral margins, the radial grooves distinct. Abdomen yellow, with blackish herringbone patterns. Palp as in Fig.
Leg spination in male:
Fe | Pa | Ti | Mt | Ta | |
I | 3d 2p 1r | - | 1p 3-3v | 2p 3-3v | - |
II | 3d 3p 2r | 1d 1p | 2p 3-3v | 1p 3-3v | - |
III | 3d 2p 2r | 2d 1p 1r | 2d 2p 3r 3-3v | 2d 5p 5r 3-3v | 1p 1r |
IV | 3d 2p 1r | 2d 1p 1r | 2d 2p 2r 3-3v | 5p 5r 3-3v | 2p 1r |
Female (paratype): Total length 8.65. Carapace 4.10 long, 3.00 wide. Abdomen 4.55 long, 2.55 wide. Eye sizes and interdistances: AME 0.20, ALE 0.18, PME 0.18, PLE 0.15; AME-AME 0.08, AME-ALE 0.05, PME-PME 0.10, PME-PLE 0.10. Leg measurements: I: 14.25 (4.00, 5.00, 3.25, 2.00); II: 12.20 (3.50, 4.00, 3.00, 1.70); III: 11.35 (3.25, 3.75, 2.75, 1.60); IV: 15.95 (4.50, 5.10, 4.25, 2.10). Carapace reddish, with black lateral margins, the radial grooves distinct; sternum with light stripes. Abdomen black, with yellow spots and herringbone patterns. Epigyne as in Fig.
Longicoelotes geei sp. n., female paratype and male holotype. A Epigyne, ventral view B Vulva, dorsal view C Male habitus, dorsal view D Female habitus, dorsal view E Female habitus, ventral view. A = epigynal atrium, CD = copulatory duct, FD = fertilization duct, PM = posterior margin of atrium, R = receptacle. Scale bars: equal for D, E.
Leg spination in female:
Known only from Jiangxi Province of China (Fig.
Coelotes kulianganus Chamberlin, 1924: 24, pl. 5, fig. 40 (♀).
Longicoelotes
kulianganus
:
2♀ 3♂, China: Fujian Province: Fuzhou Prefecture: Guling (new name for Kuliang), Liushanwang Park, N26°05'34", E119°23'32", 725 m, 1.XII.2016, X. Zhang; 2♀ 8♂, the same locality, Yixia Villa, N26°05'32", E119°23'32", 718 m, 1.XII.2016, X. Zhang; 5♀ 3♂, the same locality, swimming pool, N26°05'33", E119°23'30", 684 m, 2.XII.2016, X. Zhang.
The male can be distinguished from other Longicoelotes species by indistinct median apophysis and short cymbial furrow, about 1/5 length of cymbium (vs 1/3 in L. geei sp. n.) (Figs
Palp of Longicoelotes kulianganus, specimen from Guling. A Prolateral view B Ventral view C Retrolateral view. CAT = anterior tip of conductor, CF = cymbial furrow, CO = conductor, CPT = posterior tip of conductor, E = embolus, EB = embolic base, MA = median apophysis, PA = patellar apophysis, ST = subtegulum, T = tegulum, TS = tegular sclerite, VTA = ventral tibial apophysis. Scale bar: equal for A, B, C.
Male: Total length 8.00. Carapace 3.50 long, 2.75 wide. Abdomen 4.50 long, 2.50 wide. Eye sizes and interdistances: AME 0.20, ALE 0.18, PME 0.19, PLE 0.15; AME-AME 0.05, AME-ALE 0.05, PME-PME 0.08, PME-PLE 0.08. Leg measurements: I: 14.75 (4.25, 4.75, 3.50, 2.25); II: 12.35 (3.50, 4.00, 3.00, 1.85); III: 11.80 (3.30, 3.50, 3.25, 1.75); IV: 16.25 (4.50, 5.00, 4.50, 2.25). Carapace greenish, the radial grooves indistinct. Abdomen grayish, with blackish herringbone patterns. Palp as in Fig.
Leg spination in male:
Fe | Pa | Ti | Mt | Ta | |
---|---|---|---|---|---|
I | 3d 2p 1r | - | 3-3v | 1p 3-3v | - |
II | 3d 1p 1r | - | 2p 3-3v | 3p 3-3v | - |
III | 3d 2p 2r | 1d 1p 1r | 2d 2p 2r 3-3v | 5p 5r 3-3v | 2p 1r |
IV | 3d 2p 1r | 1d 1p 1r | 2d 2p 2r 3-3v | 5p 5r 3-3v | 2p 1r |
Female: Total length 6.75. Carapace 2.75 long, 2.15 wide. Abdomen 4.00 long, 2.25 wide. Eye sizes and interdistances: AME 0.15, ALE 0.15, PME 0.16, PLE 0.13; AME-AME 0.06, AME-ALE 0.05, PME-PME 0.10, PME-PLE 0.10. Leg measurements: I: 10.50 (3.00, 3.50, 2.50, 1.50); II: 9.20 (2.75, 3.00, 2.25, 1.20); III: 8.70 (2.50, 2.70, 2.25, 1.25); IV: 12.00 (3.50, 3.75, 3.25, 1.50). Carapace greenish, with black lateral margins, the radial grooves distinct; sternum with light stripes. Abdomen black, with yellow spots and herringbone patterns. Epigyne as in Fig.
Longicoelotes kulianganus, specimen from Guling. A Epigyne, ventral view B Vulva, dorsal view C Male habitus, dorsal view D Female habitus, dorsal view E Female habitus, ventral view. A = epigynal atrium, CD = copulatory duct, FD = fertilization duct, H = epigynal hood, PM = posterior margin of atrium, R = receptacle. Scale bars: equal for D, E.
Leg spination in female:
The manuscript benefitted greatly from comments by Yuri M. Marusik (Magadan, Russia) and Alexander A. Fomichev (Barnaul, Russia). Joseph K. H. Koh (Singapore) checked the language. This study was supported by the National Natural Sciences Foundation of China (31471960, 31530067) and the Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences (2015CASEABRI005, Y4ZK111B01).