Research Article |
Corresponding author: Yan-Li Che ( shirleyche2000@126.com ) Academic editor: Fred Legendre
© 2024 Qian-Qian Li, Wen-Wen Yao, Ke Zhang, Zong-Qing Wang, Yan-Li Che.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Li Q-Q, Yao W-W, Zhang K, Wang Z-Q, Che Y-L (2024) Six new species of Margattea Shelford, 1911 (Blaberoidea, Pseudophyllodromiidae, Neoblattellini) from China. ZooKeys 1191: 339-367. https://doi.org/10.3897/zookeys.1191.113147
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Six Margattea species are established and described: three are cryptic species, namely, M. parabisignata Li & Che, sp. nov., M. semicircularis Li & Che, sp. nov., and M. forcipata Li & Che, sp. nov. They are distinguished from known species M. bisignata, M. spinifera, and M. paratransversa by their male genitalia with the aid of molecular species delimitation method (ABGD) using COI as the molecular marker. The other three new species are M. pedata Li & Che, sp. nov., M. undulata Li & Che, sp. nov., and M. bisphaerica Li & Che, sp. nov. Morphological and genitalia photographs of these new species of Margattea, as well as a key to the species of Margattea from China, are provided.
ABGD, DNA barcoding, Margattea, new species
A total of 63 species of the genus Margattea in Neoblattellini have been recorded in Asia, Africa, and parts of Oceania (
At present, members of Margattea are identified by the simple, cylindrical, and symmetrical styli, the usually specialized eighth abdominal tergum, the pronotal disc with symmetrical stripes and maculae, and the median phallomere usually with accessory structure (
DNA barcoding (
All type specimens are deposited in College of Plant Protection, Southwest University, Chongqing, China (SWU). Male genital segments were immersed in 10% NaOH solution and incubated with water at 90 °C for 15 minutes to dissolve the fat. All segments were dissected and stored in glycerol for observation, and preserved along with the remainder of the specimen which is stored in ethyl alcohol. All photos were taken by a Leica DFC digital microscope camera attached to a Leica M205A stereomicroscope, then modified with Adobe Photoshop CC 2019. Specimens examined were measured by Vernier Caliper. Morphological terminology mainly follows
ScP subcosta posterior;
R radius;
RA radius anterior;
RP radius posterior;
Pcu postcubitus;
M media;
CuA cubitus anterior;
CuP cubitus posterior;
V vannal.
Total DNA was obtained from legs and thoracic muscle using the Hipure Tissue DNA Mini Kit, and the remainder of the specimen was stored in ethyl alcohol. The primers were used to amplify the 658 bp cytochrome c oxidase subunit I (COI) fragment (Table
A total of 105 COI sequences were analyzed, of which 53 sequences are obtained in this study and 52 sequences were downloaded from GenBank (Balta vilis, Sorineuchora nigra, and Mantis religiosa were selected as outgroups) (Table
Species | Voucher number | Accession number | Location |
---|---|---|---|
Ingroups | |||
M. angusta | MW970280 | ||
KY349624 | |||
M. bicruris | EX_1 | PP135569 | Mengla, Xishuangbanna, Yunnan |
EX_2 | PP135570 | ||
MW970303 | |||
M. bisignata | SY_1 | PP135579 | Dabie Mountain, Huanggang, Hubei |
SY_3 | PP135580 | Tangkou, Huangshan, Anhui | |
SY_4 | PP135581 | Jinyun Mountain, Beibei, Chongqing | |
SY_5 | PP135582 | Huangtangxi, Quanzhou, Fujian | |
SY_6 | PP135583 | Yinshan Park, Jinxiu, Guangxi | |
SY_7 | PP135584 | Mangshan Forest Park, Hunan | |
SY_8 | PP135585 | Liupan, Jinhua, Zhejiang | |
SY_9 | PP135586 | Huanglong Mountain, Lushan, Jiangxi | |
SY_10 | PP135587 | Fanjing Mountain, Tongren, Guizhou | |
SY_11 (F) | PP135588 | E’mei Mountain, Leshan, Sichuan | |
MW970310 | |||
MW970317 | |||
MW970315 | |||
KY349596 | |||
KY349607 | |||
KY349603 | |||
KY349604 | |||
M. bisphaerica sp. nov. | SP1 | PP135563 | Shengtang Mountain, Jinxiu, Guangxi |
Q5_34 (F) | PP135562 | ||
M. caudata | WB_3 | PP135610 | Meizi Lake, Pu’er, Yunnan |
MW970283 | |||
MW970284 | |||
M. concava | AY_1 | PP135572 | Diaoluo Mountain, Lingshui, Hainan |
AY_3 (F) | PP135574 | ||
AY_4 | PP135575 | ||
AY_2 | PP135573 | Menglun, Xishuangbanna, Yunnan | |
AY_5 | PP135576 | Maogan, Baoting, Hainan | |
KY349650 | |||
MW970253 | |||
KY349647 | |||
MW970254 | |||
MW970252 | |||
M. cuspidata | MW970300 | ||
MW970301 | |||
M. deltodonta | ZT_3 | PP135609 | Dawei Mountain, Pingbian, Yunnan |
MW970294 | |||
M. deltodonta | ZT_3 | MW970295 | Dawei Mountain, Pingbian, Yunnan |
M. disparilis | MW970290 | ||
MW970291 | |||
MW970292 | |||
M. forcipata sp. nov. | HD_3 | PP135604 | Golden Gully, Zhaoqing, Guangdong |
SHD_1 | PP135605 | ||
SP8 (F) | PP135606 | ||
M. limbata | MW970281 | ||
M2 (F) | PP135607 | Dushan, Qiannan, Guizhou | |
M. mckittrickae | MS_3 | PP135612 | Diaoluo Mountain, Lingshui, Hainan |
M. multipunctata | DB_1 (F) | PP135566 | Menglun, Xishuangbanna, Yunnan |
DB_2 (F) | PP135567 | ||
DB_3 | PP135568 | ||
KY349646 | |||
MW970269 | |||
M. nimbata | KY349658 | ||
MW970261 | |||
MW970259 | |||
KY349653 | |||
M. parabisignata sp. nov. | SY_2 | PP135600 | Limu Mountain, Qiongzhong, Hainan |
SP4 | PP135598 | ||
SP5 (F) | PP135599 | ||
M. paratransversa | MW970262 | ||
MW970263 | |||
M. pedata sp. nov. | NZ_3 (F) | PP135564 | Nabang, Yinjiang, Yunnan |
NZ_4 (F) | PP135565 | ||
M. perspicillaris | M7 | PP135578 | Yinggeling, Baisha, Hainan |
H_2 | PP135577 | ||
M. semicircularis sp. nov. | CY_7 | PP135595 | Baishaogou, Zunyi, Guizhou |
SP9 | PP135596 | ||
SP10 (N) | PP135597 | ||
M. speciosa | HL_3 | PP135571 | Libo, Qiannan, Guizhou |
KY349620 | |||
KY349618 | |||
MW970279 | |||
M. spinifera | CY_1 | PP135589 | Diaoluo Mountain, Lingshui, Hainan |
CY_2 | PP135590 | ||
CY_3 | PP135591 | Wuyi Mountain, Wuyishan, Fujian | |
CY_4 | PP135592 | Dayao Mountain, Jinxiu, Guangxi | |
CY_6 | PP135593 | Menglun, Xishuangbanna, Yunnan | |
M1 (F) | PP135594 | Maolan National Forest Park, Guizhou | |
KY349628 | |||
MW970274 | |||
KY349636 | |||
M. spinifera | M1 (F) | KY349639 | Maolan National Forest Park, Guizhou |
MW970278 | |||
M. spinosa | DC_1 (F) | PP135611 | Wuzhi Mountain, Wuzhishan, Hainan |
MW970299 | |||
KY349617 | |||
KY349613 | |||
KY349615 | |||
KY349610 | |||
M. transversa | MW970264 | ||
MW970265 | |||
KY349659 | |||
M. trispinosa | SC_3 (F) | PP135614 | Butterfly Valley, Honghe, Yunnan |
M4 | PP135613 | ||
M. undulata sp. nov. | SP_2 | PP135602 | Jinyun Mountain, Beibei, Chongqing |
Q1_29 | PP135601 | ||
SP6 (F) | PP135603 | ||
Outgroups | |||
Balta vilis | KT279743 | ||
Mantis religiosa | KM29415 | ||
Sorineuchora nigra | MF612149 |
We used a molecular species delimitation method (ABGD:
Combining the external morphological character, we identified 22 morphospecies of Margattea from a large number of samples collected, including three new species, M. pedata Li & Che, sp. nov., M. undulata Li & Che, sp. nov., and M. bisphaerica Li & Che, sp. nov. (Fig.
In this study, we acquired 105 COI sequences of Margattea representing 22 morphospecies of Margattea. The ML phylogenetic tree showed that samples (including males, females, and nymphs) of the same morphospecies form monophyletic groups, although most of the nodes did not have high bootstrap values (Fig.
Eighteen of 22 morphological species were well supported by the ABGD result. M. angusta Wang, Li, Wang & Che, 2014 and M. mckittrickae Wang, Che & Wang, 2009 were considered as one MOTU.
A M. spinifera Bey-Bienko, 1958, median phallomere, dorsal view (CY_2) B M. semicircularis Li & Che, sp. nov., median phallomere, dorsal view (CY_7) C M. bisignata Bey-Bienko, 1970, left phallomere, dorsal view (SY_3) D M. parabisignata Li & Che, sp. nov., left phallomere, dorsal view (SY_2) E M. paratransversa He & Wang, 2021, subgenital plate and median phallomere, dorsal view (MW970262) F M. forcipata Li & Che, sp. nov., subgenital plate and median phallomere, dorsal view (SHD_1). Scale bars: 0.5 mm.
Margattea
Shelford, 1911: 155. Type species: Blatta ceylanica Saussure, 1868; by monotypy. Rehn 1931: 302; Bey-Bienko 1938: 121; Bey-Bienko 1950: 145; Princis 1969: 862;
Kuchinga Hebard, 1929: 41. Type species: Phyllodromia longealata Brunner von Wattenwyl, 1898; by selection. Hanitsch 1931: 392. Synonymized by Bey-Bienko 1938: 121. Princis 1969: 862.
Theganosilpha
Kumar & Princis, 1978: 33. Type species: Theganopteryx perspicillaris Karny, 1915; by monotypy. Asahina 1979: 119. Synonymized by
Molestella Bruijning, 1948: 74. Type species: Phyllodromia molesta Brunner von Wattenwyl & Bruijning, 1948; by monotypy. Princis 1969: 803. Synonymized by Roth 1991: 980.
Margattina Bey-Bienko, 1958: 675. Type species: Margattina trispina Bey-Bienko, 1958. Synonymized by Liu et Zhou 2011: 936.
Body small, usually yellowish brown. Interocellar distance slightly wider than the distance between eyes, narrower than the distance between antennal sockets. The fifth maxillary palp expanded, the third and fourth palpi both longer than the fifth palp. Pronotum subelliptical, broader than long, the disc usually with symmetrical maculae and stripes. Tegmina and hind wings fully developed, mostly both extending beyond the end of abdomen. Tegmina with M and CuA radial, M straight with 4–7 branches. Hind wings with ScP and RA expanded at apex, CuA with 4–6 complete branches. Anteroventral margin of front femur Type B2 or B3. Four proximal tarsomeres with pulvilli. The pretarsi with arolium, tarsal claws symmetrical and specialized, with minute denticles on ventral margins. Eighth abdominal tergum usually specialized, with a tuft of setae in the middle near posterior margin. Supra-anal plate usually short and transverse, paraprocts similar and flaky. Cerci long, with setae on the ventral surface. Male subgenital plate symmetrical or slightly asymmetrical. Styli symmetrical and cylindrical, rarely asymmetrical or non-cylindrical. Male genitalia. Left phallomere small, irregularly bone-shaped, mostly with spine-like process. Median phallomere slender, rod-shaped, the apex irregular and variable; accessory sclerite complicated, generally arched. Hook phallomere on right side, apex usually curved inwards.
The genus Margattea is supposedly closely related to Chorisoserrata (
The genus Margattea can be distinguished from Chorisoserrata (parts of Asia and Indonesia) by the following characteristics: 1) anteroventral margin of front femur Type B2 or B3, in contrast to C2 (but rarely B3) in Chorisoserrata; 2) eighth abdominal tergum usually specialized, with a tuft of setae in the middle near posterior margin; while in the latter, abdominal terga unspecialized.
1 | Tegmina not extending beyond the end of abdomen | 2 |
– | Tegmina extending beyond the end of abdomen | 3 |
2 | Tegmina reaching the middle of abdomen | M. hemiptera Bey-Bienko, 1958 |
– | Tegmina extending beyond the middle of the abdomen but not reaching the end of abdomen | M. perspicillaris (Karny, 1915) |
3 | Pronotum without maculae | M. immaculata Liu & Zhou, 2011 |
– | Pronotum with maculae | 4 |
4 | The distance between eyes narrow, nearly half of interocellar distance | M. angusta Wang, Li, Wang & Che, 2014 |
– | The distance between eyes wide, wider than half of interocellar distance | 5 |
5 | Anteroventral margin of front femur Type B3 | 6 |
– | Anteroventral margin of front femur Type B2 | 15 |
6 | Interstylar region nearly truncate, not produced | 7 |
– | Interstylar region obviously produced | 9 |
7 | Styli conical | 8 |
– | Styli foot-shaped | M. pedata Li & Che, sp. nov. |
8 | Median phallomere with three spinelike sclerites | M. trispinosa (Bey-Bienko, 1958) |
– | Median phallomere with small spines | M. mckittrickae Wang, Che & Wang, 2009 |
9 | The trailing edge of interstylar region curls upward | M. furcata Liu & Zhou, 2011 |
– | The trailing edge of interstylar region no curls upward | 10 |
10 | Interstylar margin semicircular produced | 11 |
– | Interstylar margin not semicircular produced | 12 |
11 | Left phallomere with two small spines | M. semicircularis sp. nov. |
– | Left phallomere with three spine-like processes | M. spinifera Bey-Bienko, 1958 |
12 | Two sides of interstylar protrusion curled | 13 |
– | Two sides of interstylar protrusion not curled | 14 |
13 | Interstylar region convex fishtail-shaped | M. caudata He & Wang, 2021 |
– | Interstylar region convex irregular | M. disparilis He & Wang, 2021 |
14 | Accessory sclerite with a bristle brush at right apex | M. cuspidata He & Wang, 2021 |
– | Accessory sclerite without a bristle brush at right apex | M. flexa Wang, Li, Wang & Che, 2014 |
15 | Head dark brown or reddish brown | 16 |
– | Head yellowish brown | 17 |
16 | Styli dissimilar | M. pseudolimbata Wang, Li, Wang & Che, 2014 |
– | Styli similar | M. limbata Bey-Bienko, 1954 |
17 | Pronotal disc with white maculae | M. multipunctata Wang, Che & Wang, 2009 |
– | Pronotal disc with brown maculae | 18 |
18 | Interstylar region concave | 19 |
– | Interstylar region not concave | 20 |
19 | Styli symmetrical, conical | M. concava Wang, Che & Wang, 2009 |
– | Styli asymmetrical, the left shorter than the right | M. bisphaerica Li & Che, sp. nov. |
20 | Eighth abdominal tergum unspecialized | 21 |
– | Eighth abdominal tergum specialized | 22 |
21 | Posterior margin of supra-anal plate with sharp protrusions | M. producta Wang, Che & Wang, 2009 |
– | Posterior margin of supra-anal plate without sharp protrusions | M. punctulata (Brunner von Wattenwyl, 1893) |
22 | Interstylar region with triangular protrusion | M. deltodonta He & Wang, 2021 |
– | Interstylar region without triangular protrusion | 23 |
23 | Left phallomere without rodlike structure | 24 |
– | Left phallomere with rodlike structure | 25 |
24 | Apex of median phallomere with sparse brush-like structure composed of similar spines | M. bisignata Bey-Bienko, 1970 |
– | Apex of median phallomere with sparse brush-like structure composed of uneven spines | M. parabisignata Li & Che, sp. nov. |
25 | Body overall length not greater than 9.0 mm | 26 |
– | Body overall length greater than 12.0 mm | 27 |
26 | Median phallomere with spinelike sclerite | M. nimbata (Shelford, 1907) |
– | Median phallomere without spinelike sclerite | M. spinosa Wang, Li, Wang & Che, 2014 |
27 | Median phallomere with brush structure at apex | 28 |
– | Median phallomere without brush structure at apex | 29 |
28 | Interstylar margin sinuate | M. undulata Li & Che, sp. nov. |
– | Interstylar margin not sinuate | M. speciosa Liu & Zhou, 2011 |
29 | Accessory sclerite of median phallomere with a transverse rod | 30 |
– | Accessory sclerite of median phallomere without a transverse rod | M. bicruris He & Wang, 2021 |
30 | Apex of median phallomere enlarged, forceps | M. forcipata Li & Che, sp. nov. |
– | Apex of median phallomere with a curved long spine | 31 |
31 | Left phallomere with three spines | M. paratransversa He & Wang, 2021 |
– | Left phallomere with four spines | M. transversa He & Wang, 2021 |
Holotype : China • ♂; Yunnan Province, Dehong Dai and Jingpo Autonomous Prefecture, Yingjiang County, Nabang Town; 282 m; 17 Aug. 2015; Xin-Ran Li, Zhi-Wei Qiu leg; SWU-B-PS000001. Paratypes: China • 1 ♂ & 1 ♀; same data as holotype; SWU-B-PS000002–000003 • 5 ♂ & 2 ♀; Yunnan Province, Dehong Dai and Jingpo Autonomous Prefecture, Yingjiang County, Nabang Town; 282 m; 11 Jul. 2012; Dong Wang leg; SWU-B-PS000004–000010.
(mm). Male (n = 6), pronotum length × width: 2.4–2.7 × 3.4–3.7, tegmina length: 11.8–12.3, body length: 8.8–10.3, overall length: 13.2–13.7. Female (n = 4), pronotum length × width: 2.4–2.9 × 3.2–3.9, tegmina length: 11.2–11.6, body length: 10.9–12.8, overall length: 13.2–14.0.
Male. Coloration. Body pale yellow (Fig.
Margattea pedata Li & Che, sp. nov. A, B, E–O male C, D female A holotype, dorsal view B holotype, ventral view C paratype, dorsal view D paratype, ventral view E pronotum, dorsal view F head, ventral view G tegmen, ventral view H hind wing, ventral view I eighth abdominal tergum, ventral view J maxillary palpi segments 3–5 K front femur, ventral view L supra-anal plate, ventral view M left phallomere, ventral view N subgenital plate and median phallomere, ventral view O hook phallomere, ventral view. Scale bars: 5 mm (A–D, G, H); 1 mm (E, F, I–L, N); 0.5 mm (M, O).
Head. Vertex slightly exposed, interocellar distance slightly wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Abdomen and genitalia. Eighth abdominal tergum specialized, with a heart-shaped transparent area and a tuft of bristles in the middle (Fig.
Female. Similar to the male (Fig.
This species is similar to M. speciosa Liu & Zhou, 2011 in general appearance, but can be differentiated from the latter by the following characters: 1) styli foot-shaped, while in the latter conical; 2) left phallomere with a long, curved spine, absent in the latter; and 3) accessory sclerite I without a brush-like structure at apex, while in the latter, accessory sclerite I with a brush-like structure at apex.
The specific epithet is derived from the Latin word pedatus, referring to the foot-shaped styli.
China (Yunnan).
Holotype : China • ♂; Guangxi Zhuang Autonomous Region, Laibin City, Jinxiu Yao Autonomous County, Mountain Shengtang; 1182 m; 5 Jun. 2014; Shun-Hua Gui, Xin-Ran Li leg; SWU-B-PS000011. Paratypes: China • 3 ♂ & 1 ♀; same data as holotype; SWU-B-PS000012–000015 • 1 ♂; Guangxi Zhuang Autonomous Region, Laibin City, Jinxiu Yao Autonomous County, Mountain Shengtang; 400 m; 13 Jul. 2015; Lu Qiu, Qi-Kun Bai leg; SWU-B-PS000016.
(mm). Male (n = 6), pronotum length × width: 2.3–2.5 × 3.0–3.4, tegmina length: 11.8–12.5, body length: 9.8–11.6, overall length: 13.8–14.9. Female (n = 2), pronotum length × width: 2.3–2.5 × 3.3, tegmina length: 10.7–11.4, body length: 10.3–11.0, overall length: 13.8–13.9.
Male. Coloration. Body brown (Fig.
Margattea bisphaerica Li & Che, sp. nov. A, B, E–O male C, D female A holotype, dorsal view B holotype, ventral view C paratype, dorsal view D paratype, ventral view E pronotum, dorsal view F head, ventral view G tegmen, ventral view H hind wing, ventral view I eighth abdominal tergum, ventral view J maxillary palpi segments 3–5 K front femur, ventral view L supra-anal plate, ventral view M left phallomere, ventral view N subgenital plate and median phallomere, ventral view O hook phallomere, ventral view. Scale bars: 5 mm (A–D, G, H); 1 mm (E, F, I–K, N); 0.5 mm (L, M, O).
Head. Vertex slightly exposed, interocellar distance slightly much wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Abdomen and genitalia. Eighth abdominal tergum specialized, with a tuft of bristles in the middle (Fig.
Female. Similar to the male. Subgenital plate symmetrical, middle posterior margin concave inward (Fig.
This species is similar to M. concava Wang, Che & Wang, 2009 in general appearance, but can be differentiated from the latter by the following characters: 1) styli dissimilar and spherical, the left stylus significantly smaller than the right stylus; while in the latter, styli similar and conical; 2) left phallomere with a slender curved spine, absent in the latter.
The specific name is derived from the Latin words, bi and sphaericus, referring to the dissimilar and spherical styli.
China (Guangxi).
Holotype : China • ♂; Chongqing City, Beibei District, Mountain Jinyun; 550 m; 12 Jul. 2016; Lu Qiu, Zhi-Wei Qiu leg; SWU-B-PS000017. Paratypes: China • 10 ♂ & 1 ♀; same data as holotype; SWU-B-PS000018–000028 • 2 ♂ & 1 ♀; Chongqing City, Jiangjin District, Mountain Simian; 425 m; 21 Sep. 2007; Wei-Wei Zhang leg; SWU-B-PS000029–000031 • 1 ♂ & 1 ♀; Chongqing City, Liangping District, Dongshan Forest Park; 2 Oct. 2007; Wei-Wei Zhang leg; SWU-B-PS000032–000033.
(mm). Male (n = 4), pronotum length × width: 2.4–2.9 × 3.6–3.8, tegmina length: 12.8–13.6, body length: 10.4–12.1, overall length: 14.9–16. Female (n = 4), pronotum length × width: 2.3–2.5 × 3.3, tegmina length: 10.7–11.4, body length: 10.3–11.0, overall length: 13.8–13.9.
Male. Coloration. Body, head and face yellowish brown (Fig.
Margattea undulata Li & Che, sp. nov. A, B, E–O male C, D female A holotype, dorsal view B holotype, ventral view C paratype, dorsal view D paratype, ventral view E pronotum, dorsal view F head, ventral view G tegmen H hind wing I eighth abdominal tergum, ventral view J maxillary palpi segments 3–5 K front femur, ventral view L supra-anal plate, ventral view M left phallomere, ventral view N subgenital plate and median phallomere, ventral view O hook phallomere, ventral view. Scale bars: 5 mm (A–D, G, H); 1 mm (E, F, I–L, N); 0.5 mm (M, O).
Head. Vertex slightly exposed, interocellar distance slightly wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Female. Similar to the male but body and wings somewhat shorter (Fig.
This species is similar to M. flexa Wang et al., 2014 in general appearance, but can be differentiated from the latter by the following characters: 1) interstylar margin sinuate, left side with 4–6 small spines, right side with 4–7 small spines; while in the latter, interstylar margin strongly produced, whose lateral sides upturned and scattered with spines; 2) left phallomere irregular bone-shaped, without a small spine; while in the latter, left phallomere irregular bone-shaped, with two spines; 3) accessory sclerite II with three lamellar structures with small spines; while in the latter, accessory sclerite II with lamellar structure without small spines.
The specific name is derived from the Latin word undulatus, which refers to the sinuate interstylar margin.
China (Chongqing).
Holotype : China • ♂; Guizhou Province, Zunyi City, Suiyang County, Qingbantang Town, Baishao Ditch; 30 Jul. 2013; Xiu-Dan Wang leg; SWU-B-PS000034. Paratype: China • 1 ♂; same data as holotype; SWU-B-PS000035.
(mm). Male (n = 2), pronotum: length × width 2.3–2.5 × 3.0–3.4, tegmina length: 10.7–11.2, body length: 10.5–11.0, overall length: 12.9–13.2.
Male. Coloration. Body, head and face yellowish brown (Fig.
Margattea semicircularis Li & Che, sp. nov. A–N male A holotype, dorsal view B holotype, ventral view C pronotum, dorsal view D head, ventral view E eighth abdominal tergum, ventral view F tegmen, ventral view G hind wing, ventral view H maxillary palpi segments 3–5 I front femur, ventral view J Left phallomere, dorsal view K supra-anal plate, ventral view L median phallomere, ventral view M subgenital plate, ventral view N hook phallomere, ventral view. Scale bars: 5 mm (A, B, F, G); 1 mm (C–E, H, I, K–M); 0.5 mm (J, N).
Head. Vertex slightly exposed, interocellar distance wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Abdomen and genitalia. Eighth abdominal tergum specialized, with a heart-shaped transparent area and a tuft of bristles in the middle (Fig.
This species is similar to M. spinifera Bey-Bienko, 1958a in general appearance, but can be differentiated from the latter by the following characters: 1) left phallomere with two small spines; while the latter, left phallomere with three spine-like processes; 2) accessory sclerite I arched, left end trigonate; while in the latter, accessory sclerite I arched, left end expanded with fuzz; 3) accessory sclerite II long transverse, and with two lamellar structures with a row of spines, while in the latter, without other accessory sclerites.
The scientific name is derived from the Latin word semicircularis, which indicates the interstylar margin has a semicircular protrusion.
China (Guizhou).
Holotype : China • ♂; Hainan Province, Qiongzhong Li and Miao Autonomous County, Mountain Limu; 600 m; 16 May. 2015; Xin-Ran Li, Zhi-Wei Qiu leg; SWU-B-PS000036. Paratypes: China • 2 ♂ & 1 ♀; same data as holotype; SWU-B-PS000037–000039 • 7 ♂ & 3 ♀; Hainan Province, Qiongzhong Li and Miao Autonomous County, Mountain Limu; 600 m; 16 May. 2015; Xin-Ran Li, Zhi-Wei Qiu leg; SWU-B-PS000040–000049.
(mm). Male (n = 7), pronotum length × width: 2.2–2.8 × 3.0–3.6, tegmina length: 11.3–12.2, body length: 10.1–11.1, overall length: 13.0–14.0. Female (n = 5), pronotum length × width: 2.2–2.8 × 3.0–3.6, tegmina length: 10.7–11.4, body length: 9.6–11.7, overall length: 12.9–13.7.
Male. Coloration. Body pale yellowish brown (Fig.
Margattea parabisignata Li & Che, sp. nov. A, B, E–O male C, D female A holotype, dorsal view B holotype, ventral view C paratype, dorsal view D paratype, ventral view E pronotum, dorsal view F head, ventral view G tegmen, ventral view H hind wing, ventral view I eighth abdominal tergum, ventral view J maxillary palpi segments 3–5 K front femur, ventral view L supra-anal plate, ventral view M left phallomere, ventral view N subgenital plate and median phallomere, ventral view O hook phallomere, ventral view. Scale bars: 5 mm (A–D, G, H); 1 mm (E, F, I–L, N); 0.5 mm (M, O).
Head. Vertex slightly exposed, interocellar distance wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Abdomen and genitalia. Eighth abdominal tergum specialized, with a sparse tuft of bristles in the middle (Fig.
Female. Similar to the male (Fig.
This species is similar to M. bisignata Bey-Bienko, 1970 in general appearance, but can be differentiated from the latter by the following characters: 1) left phallomere with a short spiny process; the latter with a long spine process; 2) median phallomere apex with sparse brush-like structure composed of spines of varying sizes; while in the latter, median phallomere curved at apex, sheet-like, and with brush-shaped structure.
The species name parabisignata reflects its similarity to M. bisignata Bey-Bienko, 1970.
China (Hainan).
Holotype : China • ♂; Guangdong Province, Zhaoqing City, Fenghuang Town, Jiukeng River, Gold Ditch; 3 Jul. 2015; Zhi-Wei Qiu, Yong-Quan Zhao leg; SWU-B-PS000050. Paratypes: China • 6 ♂ & 1 ♀; same data as holotype; SWU-B-PS000051–000057 • 1 ♂; Guangdong Province, Zhaoqing City, Fenghuang Town, Jiukeng River, Lakeside Villa; 4 Jul. 2015; Zhi-Wei Qiu, Yong-Quan Zhao leg; SWU-B-PS000058.
(mm). Male (n = 4), pronotum length × width: 2.4–2.6 × 3.2–3.4, tegmina length: 10.5–11.5, body length: 10.4–10.8, overall length: 13.1–13.4. Female (n = 2), pronotum length × width: 2.5–2.7 × 3.4–3.6, tegmina length: 11.1–11.5, body length: 10.6–10.7, overall length: 13.4–13.7.
Male. Coloration. Body, head and face yellowish brown (Fig.
Margattea forcipata Li & Che, sp. nov. A, B, E–O male C, D female A holotype, dorsal view B holotype, ventral view C paratype, dorsal view D paratype, ventral view E pronotum, dorsal view F head, ventral view G tegmen, ventral view H hind wing, ventral view I eighth abdominal tergum, ventral view J maxillary palpi segments 3–5 K front femur, ventral view L supra-anal plate, ventral view M left phallomere, ventral view N subgenital plate and median phallomere, ventral view O hook phallomere, ventral view. Scale bars: 5 mm (A–D, G, H); 1 mm (E, F, I–L, N); 0.5 mm (M, O).
Head. Vertex slightly exposed, interocellar distance wider than the distance between eyes, narrower than the distance between antennal sockets (Fig.
Abdomen and genitalia. Eighth abdominal tergum specialized, with a tuft of bristles in the middle (Fig.
Female. Similar to the male.
This species is similar to M. transversa He & Wang, 2021 in general appearance, but can be differentiated from the latter by the following characters: 1) left phallomere with a long spine; the latter with three long spine-like processes; 2) median phallomere with a forceps-shaped apex; while in the latter, median phallomere apex with a curved spine.
The specific name forcipatus, derived from Latin, refers to the median phallomere with a forceps-shaped apex.
China (Guangdong).
The intraspecific and interspecific genetic distances are considerably high in Margattea (Suppl. material
In this study, we initially determined three morphospecies, namely “M. spinifera”, “M. bisignata”, and “M. paratransversa”, whose individuals are almost indistinguishable. In contrast, these morphospecies are each divided into two MOTUs in molecular species delimitation. We hence examined the male genitalia of different samples from each of these morphospecies and found differences in the accessory sclerite I of “M. spinifera” (Fig.
We would like to express gratitude to all specimen collectors, as well as Xiu-Dan Wang, Xin-Ran Li, Zhi-Wei Qiu, Shun-Hua Gui, Qi-Kun Bai, Yong-Quan Zhao, and Lu Qiu who provided the information about field observations. We are especially grateful to Wen-Bo Deng, Yi-Shu Wang, and Xin-Xing Luo for their suggestions. We also sincerely thank Fred Legendre and Zuzana Varadínová for their valuable suggestions on our manuscript.
The authors have declared that no competing interests exist.
No ethical statement was reported.
This study is supported by a Program of the Ministry of Science and Technology of the People’s Republic of China (2022FY202100) and the National Natural Sciences Foundation of China (No. 32070468, 32170458, and 31872271).
Qianqian Li: Data curation (equal); methodology (lead); visualization (equal); writing – original draft (lead); writing – review and editing (equal). Wenwen Yao: Data curation (equal); visualization (supporting); writing – review and editing (supporting); Ke Zhang: Data curation (equal); visualization (supporting);Zongqing Wang: Funding acquisition (equal); methodology (supporting); project administration (equal); supervision (equal); writing – review and editing (equal); Yanli Che: Funding acquisition (equal); project administration (equal); supervision (equal); writing – review and editing (equal).
Qian-Qian Li https://orcid.org/0009-0007-3088-9591
Zong-Qing Wang https://orcid.org/0000-0001-9413-1105
Yan-Li Che https://orcid.org/0000-0003-3214-9494
All of the data that support the findings of this study are available in the main text or Supplementary Information.
Interspecific and intraspecific genetic distances
Data type: xls