A new Muricea species (Cnidaria, Anthozoa, Octocorallia) from the eastern tropical Pacific

Odalisca Breedy; Hector M. Guzman

doi:10.3897/zookeys.629.10828

Abstract

The genus Muricea is considered abundant and widely distributed along the eastern Pacific. Its occurrence in shallow waters has been recognised; however species from deeper than 30 m have been rarely recorded. During the 2005 R/V Urracá expedition along the north and central Pacific coast of Costa Rica several octocoral specimens were collected by bottom trawling from 30 to 150 m yielding new species and new records. Herein we describe a new species of Muricea from deeper than 30 m. The morphological characters of the species were analysed and illustrated by optic and scanning electron microscopy. Muricea subtilis sp. n. can be distinguished from the other species in the genus by its thin spiny branches, non-imbricate calyces, white colony and sclerites, and the size and composition of sclerites. Comparative character tables are provided for the closest Muricea species-group. This new species increases the number in the genus to 26, and contributes to the knowledge on the diversity and distribution of mesophotic soft corals in the eastern Pacific.

Keywords

Alcyonacea , Cnidaria , eastern Pacific, mesophotic zone, Muricea subtilis , new species, plexaurid, soft corals, taxonomy

Introduction

The genus Muricea is considered abundant and widely distributed in shallow waters (< 30 m) along the eastern Pacific and was recently revised and updated to contain 25 valid species (Breedy and Guzman 2015, 2016). Muricea has been reported from Cape Hatteras, North Carolina to Brazil, including Bahamas, Greater and Lesser Antilles, and Caribbean islands (Bayer 1961); it also occurs in the eastern Pacific from southern California to Perú and presumably in Chile (Breedy and Guzman 2016).

Muricea midas Bayer, 1959 is the deepest record for the genus, at 201 m in the western Atlantic (Bayer 1959); and Muricea fruticosa Verrill, 1869, is known to 102 m in the eastern Pacific. Muricea galapagensis Deichmann, 1936, known from 94 m, was only once collected. Normally, the genus occurs shallower from one meter in intertidal zones to 30 m deep (Breedy and Guzman 2016). However, several species have been found in deeper mesophotic zones requiring further exploration and taxonomic work.

According to Breedy and Guzman (2016) boundaries among species of Muricea (as in many other octocorals) are difficult to draw. However, the morphological characters such as colony and sclerite shapes, sizes and colours still represent a valid approach to determine species together with field observation (e.g. habitat, bathymetry). The genus was divided in four groups according to the morphology of colonies and sclerites: the Muricea squarrosa species-group, Muricea fruticosa species-group, the Muricea austera species-group and the Muricea plantaginea species-group (Breedy and Guzman 2015, 2016).

Herein we describe a new mesophotic Muricea species collected during the 2005 R/V Urracá-STRI expedition to the Pacific coast of Costa Rica, that resulted in interesting material from deeper waters (see Vargas-Castillo 2008).

Material and methods

The specimens were collected by bottom trawling from unexplored habitats down to 70 m deep in the middle mesophotic zone (from 40 to 150 m), on board of the Smithsonian Tropical Research Institute R/V Urracá along the north and central Pacific coast, from Santa Elena Bay to the Nicoya Gulf.

The specimens were fixed in 70% ethanol or air-dried. For microscopic study, they were prepared according to the protocol described by Breedy and Guzman (2002), and observed using optic microscopy, Olympus LX 51 inverted microscope, and scanning electron microscopy, with a Hitachi 3700 at the Research Center of Microscopic Structures (CIEMIC) of the University of Costa Rica (UCR) and a Zeiss EVO 40 at the Electron Microscopy Laboratory (Tupper Research and Conference Center). The holotype and paratypes are deposited in the Museo de Zoología, Universidad de Costa Rica (MZUCR).

The taxonomic approach was by the evaluation of characters following Breedy and Guzman (2015, 2016). Morphological characters of colonies and sclerites are presented in Tables 1–2 and comparison with the type material of the related taxa in the genus. Measurements of branches are given taking in account the length of the calyces whether preserved in ethanol or dry. Terminology used in descriptions mostly follows Bayer et al. (1983) and Breedy and Guzman (2015, 2016).

Table 1.

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Diagnostic characters of sclerites in the Muricea plantaginea species-group. Measurements given are from the holotypes and lectotypes, in mm.

Species	Sclerite colours	Anthocodial sclerite colours	Dominant type of coenenchymal and calycular sclerites	Coenenchymal and calycular spindles maximum size	Anthocodial maximum size
M. plantaginea	rb, amb/w	lo, lb/w	ls	1×0.2	0.25×0.08
M. californica	ro, ly, amb	lo	ls	0.54×0.2	0.23×0.06
M. mortensenii	w	w	s	0.7×0.12	0.21×0.08
M. subtilis sp. n.	w	w	ls	0.93×0.14	0.20×0.05

Table 2.

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Diagnostic characters of colony morphology in the Muricea plantaginea species-group. Measurements given are from holotypes and lectotypes, in mm.

Species	Colony colour	Colony shape	Branching pattern	Length of unbranched terminal branchlets	Diameter of end branchlets (mm)	Calyx height at branchlets	Calyx arrangement at branchlets
M. plantaginea	db/w	fla	irr, lat	10–50	2–3	0.7–1.2	c, imbr
M. californica	ro	bu	irr, lat	0.5–2.8	3–3.2	1.1–1.9	c, slightly imbr
M. mortensenii	py	fla	irr	2–4	2–3	0.7–1	c
M. subtilis sp. n.	py,w	lat, fla	irr, lat, dich	5–40	1.5–2	1–1.2	c

Results

Class Anthozoa Ehrenberg, 1834
Subclass Octocorallia Haeckel, 1866
Order Alcyonacea Lamouroux, 1812
Family Plexauridae Gray, 1859

Genus Muricea Lamouroux, 1821

Muricea Lamouroux, (pars.) 1821: 36; Blainville (pars) 1834: 509; Ehrenberg (pars.) 1834: 134; Dana 1846: 673; Milne Edwards and Haime 1857: 142; Kölliker 1865: 135; Verrill 1868: 411; Verrill 1869: 418–419, 450; Studer 1887: 58; Wright and Studer 1889: 93; Gorzawsky 1908: 8; Nutting 1910: 9; Kükenthal 1919: 835; 1924: 141; Riess 1929: 383–384; Aurivillius 1931: 102–104; Deichmann 1936: 99; Bayer 1956: F210; 1959: 12; 1961: 179–180; 1981: 930 (in key); 1994: 23–24; Tixier-Durivault 1970: 154; Harden 1979: 140; Hardee and Wicksten 1996: 127–128; Marques and Castro 1995: 162; Castro et al. 2010: 779; Breedy and Guzman 2015: 6–7; 2016: 7–9.

Eumuricea (pars.) Verrill, 1869: 449; Riess 1929: 397; Breedy and Guzman 2015: 6–7.

Type species

Muricea spicifera Lamouroux, 1821, by subsequent designation (Milne Edwards and Haime 1857.)

Genus diagnosis

(based on Breedy and Guzman 2016). Colonies planar or multiplanar, bushy, arborescent, laterally branched, pinnately branched, dichotomous or with long flexible branches, with some occasional branch anastomosis. Branches and branchlets upward bending almost parallel, and with about the same thickness all along, frequently with slightly enlarged tips. Coenenchyme moderately to very thick (compared to other plexaurids) with a circle of longitudinal canals surrounding the axis and dividing the coenenchyme into a thin inner layer or axial sheath, and a thicker outer layer. The outer and inner layer of coenenchyme indiscriminate, almost blended in species with thinner branches. In some species with a thin coenenchyme polyps fully retractile within prominent calyces longitudinally and closely placed all around branches and branchlets, or spaced in loose spirals around branches and branchlets. Calyces prominent, shelf-like or tubular, with prickly projecting spindles, longitudinally arranged. Base of anthocodia without sclerites or with flat rods arranged in weakly differentiated collaret and points below tentacles, or just transversely set along the neck zone of polyp. Sclerites of outer coenenchyme and of calyx mostly long, unilateral spinous spindles, often massive, sculptured on inner surface by crowded complex tubercles and on outer surface by simple spines or prickles, and in some species with a few more or less prominent coarse, prickly projections. Spindles with laterally placed spinous or leaf-like processes are the dominant type in some species. Axial sheath composed of capstans, spindles, or oval forms, and undeveloped sclerites. Sclerite colours are white, various hues of yellow, amber, orange, purple and red. Anthocodials with lower hues.

Muricea subtilis sp. n.

http://zoobank.org/23F8B95D-10AB-4AC2-A4FC-EF1326AD0765

Figures 1, 2, 3

Material

Holotype: UCR 2322 (URR 46), ethanol preserved, off Esterillos, Puntarenas, Central Pacific, Costa Rica, 09°20.940'N, 84°30.240'W–09°21.242'N, 84°30.043'W, 51.7–53 m, R. Vargas, R/V Urraca, 17 July 2016. UCR 2322A, fragment for molecular analysis in progress.

Paratypes: MZUCR-OCT 0082 (URR 44), ethanol preserved, off Punta Mala, Puntarenas, 09°22.085'N, 84°32.206'W–09°22.280'N, 84°32.037'W, 44.2–44 m, R. Vargas, 17 July 2005; MZUCR-OCT 0125 (URR 26–53), dry, off Carrillo Beach, Nicoya, Guanacaste, 09°51.264'N, 85°29.37'W–09°50.727'N, 85°29.37'W, 39–40 m, R. Vargas, R/V Urraca, 16 July 2005; MZUCR 0126 (TWL 27–36), dry, off Carrillo Beach, 09°50.013'N, 85°29.476'W–09°49.88'N, 85°29.40'W, 30–32 m, R. Vargas, R/V Urraca, 16 July 2005; MZUCR 0140 (URR 47), dry, off Esterillos, 09°20.212'N, 84°28.358'W–09°21.610'N, 84°28.275'W, 51.7–53 m, R. Vargas, R/V Urraca, 17 July 2016; UCR 2321 (URR 46), as the holotype.

Type locality

09°20.940'N, 84°30.240'W (off Esterillos, Puntarenas), 53 m in depth.

Diagnosis

Colonies spiny and delicate in appearance, fan-like or lateral. Branching irregular, mostly dichotomous, in one or two planes. Branches and branchlets thin, 1.5–2 mm in diameter, in some cases thinner, about 1 mm. Some branch pseudo-anastomosis present. Polyps mostly close together. Calyces shelf-like, prominent, up to 1.2 mm. Calyces not imbricate. Coenenchyme thin. Coenenchymal and calycular sclerites mostly leaf-like spindles up to 0.95 mm long. Anthocodial sclerites mostly irregular warty rods and thin torches, translucent or whitish. Colony colour whitish to pale yellow.

Description

The holotype is a 14.5 cm tall and 23 cm wide colony. A 15 mm long stem, 6 mm in diameter, subdivide in two main branches, 4–5 mm diameter and arise from an irregular, 15 mm diameter holdfast (Figure 1A). The branches are about the same diameter at the bottom of the colony 3–4 mm producing thinner branchlets 2–3 mm diameter up to the ends. Branching is irregular, mostly dichotomous, branches and branchlets project at angles 45°–75°and separated up to 25 mm. They spread in one plane in a fan-like colony. The branchlets are straight or curved inwards, some are anastomosed. Unbranched terminal ends are about 2 mm in diameter and up to 40 mm long. The axis is amber. The calyces are shelf-like, 1–1.2 mm long, giving a spiny appearance to the colony. They are close together, or only a few millimetres apart, 0.5–1.5 mm, and not imbricate (Figure 1B). Some branches are devoid of polyps, probably eaten by worms. Polyps are on the upper side of the elongated calyces. The calyx size and spacing vary from the larger branches to the thinner, being larger and acute, and closer placed at the branchlets and shorter, and distant at the main branches and almost absent at the stem. The coenenchyme is thin, composed of whitish and translucent sclerites, mostly of various kinds of spindles (Figure 2A–C). The coenenchyme and the calycular sclerites are mostly leaf-like spindles, 0.25–0.93 mm long, and 0.09–0.20 mm wide and spindles, 0.40–0.60 mm long and 0.06–0.10 wide (Figure 3A–C). The axial sheath is composed of spindles, 0.25–0.45 mm long and 0.04–0.07 mm wide (Figure 3D). The anthocodial sclerites are translucent irregular warty rods, thin torches, irregular short spindles, 0.05–0.2 mm long, and 0.01–0.05 mm wide (Figures 2D, 3D). The colony is whitish to pale yellow (Figure 1A–B).

Figure 1.

Muricea subtilis sp. n., UCR 2322 (holotype). A Colony B Detail of branches.

Figure 2.

Muricea subtilis sp. n., UCR 2322 (holotype). A–C Coenenchymal sclerites D Anthocodial sclerites (optic micrographs).

Figure 3.

Muricea subtilis sp. n., UCR 2322. A–B Calycular and coenenchymal sclerites C Axial sheath D Anthocodial sclerites.

The paratypes agree in all characters with the holotype; however, some colonies have thinner branchlets, about 1 mm in diameter, and the leaf-like spindles can reach 0.95 mm long.

Etymology

The adjective subtilis (L) meaning fine, thin, delicately slender, of a cutting edge, is used here, in allusion to the thin and spiny branches characteristic of the species. The term subtilis in literature combines sharpness and acuteness that imply clarity which could also evoke the white colour of the colony.

Habitat and distribution

The species has been collected from muddy-sand bottoms, together with other octocoral species such as Muricea fruticosa Verrill, 1869; Pacifigorgia senta Breedy & Guzman, 2003, and other invertebrates from 30 to 54 m deep. A few species of gorgonians were the dominant component of the catches; some specimens were attached to debris or shells that probably hold the colonies on the mud-sandy substrate. Muricea subtilis sp. n. was collected along the outer part of Nicoya Gulf and central Pacific coast of Costa Rica.

Discussion

The species belongs to the M. plantaginea species-group together with M. mortensenii and M. californica. According to Breedy and Guzman (2016) this species-group is characterised by shelf-like calyces, thin coenenchyme, thin branches and the lack of unilateral spinous spindles (as defined for the genus). The new species’ delicate spiny colony, almost immediately separates it from the others in the group. However, it is similar to M. plantaginea (Valenciennes, 1846), white variety and M. mortensenii Hickson, 1928 in the colour of the colony and sclerites. It differs from the latter in its thicker branches, shorter calyces and smaller spindles that are the dominant type of sclerites in M. mortensenii (Tables 1–2). Muricea plantaginea is distinguished from Muricea subtilis sp. n. in having thicker non-dichotomous branches, and mostly flabellate colonies with stronger structure that is evident also in small, young colonies of M. plantaginea. The imbricate calyces and larger leaf-like spindles, up to 1 mm or slightly more (Table 1–2) in M. plantaginea are also features that differentiate these two close species.

Acknowledgements

We thank Leen van Ofwegen (Netherlands Centre for Biodiversity Naturalis), Stephen Cairns (Museum of Natural History, Smithsonian Institution) and Gary Williams (California Academy of Science) for their time and suggestions to improve this publication. Also, Yolanda Camacho and Rita Vargas (UCR) for collecting the material for this study and the Smithsonian Tropical Research Institute researchers Rachel Collins and Ross Robertson who were in charge of the R/V Urracá-STRI 2005 expedition. We appreciate Jorge Ceballos (Smithsonian Tropical Research Institute), Alexander Rodríguez and Cristian Mora (UCR) for their valuable contribution in this research. Finally, we acknowledge Jorge Brenes and Annette Calvo-Shadid (UCR) who kindly helped us with the species etymology. The project was partially sponsored by the Vicerrectoría de Investigación de la Universidad de Costa Rica, project 808-B5172, and the Smithsonian Tropical Research Institute.

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