Research Article |
Corresponding author: Armen Seropian ( armen.seropiani@iliauni.edu.ge ) Academic editor: Kazunori Yoshizawa
© 2023 Armen Seropian, Eka Arsenashvili, Natalia Bulbulashvili, Anano Shubashishvili, Giorgi Iankoshvili, Mariami Todua, Ana Ananiashvili, Shota Japarashvili, Tinatin Chkhartisvhili, Aleksi Memishishi, Sopio Balkhamishvili, Beka Chitadze, Elisabeth Karalashvili, Levan Mumladze, Nils Hein, Björn Rulik.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Seropian A, Arsenashvili E, Bulbulashvili N, Shubashishvili A, Iankoshvili G, Todua M, Ananiashvili A, Japarashvili S, Chkhartisvhili T, Memishishi A, Balkhamishvili S, Chitadze B, Karalashvili E, Mumladze L, Hein N, Rulik B (2023) Into the unknown: the first barcode-assisted checklist of Psocoptera (Insecta, Psocodea) of Georgia with a census on country species richness. ZooKeys 1168: 77-105. https://doi.org/10.3897/zookeys.1168.103666
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This checklist reports 47 species of Psocoptera from 15 families and three suborders from Georgia, of which 31 species are recorded for the first time, increasing the known fauna of the country by more than 65%. Of these, 37 species have been barcoded, representing 210 Barcode Identification Numbers (BINs). An additional 14 species are expected to occur in Georgia but remain undiscovered, meaning that only ≈ 77% of the fauna is currently documented. Barcodes, comments on distributions, and images of voucher specimens are given followed by a map of the sampling sites.
Biodiversity, CaBOL, faunistics, South Caucasus
Psocoptera, known as the booklice and barklice, is an order of hemimetabolous insects having approximately 6000 described species worldwide (
Although Psocoptera has been classified as an order for much of recent history, it was shown by
Thus, to maintain monophyly, the former orders Psocoptera and Phthiraptera are now placed in the order Psocodea (
The first attempt to assess the species richness of the psocid fauna of Georgia was made by
Changes in composition of Georgian barklice fauna through time and different surveys.
Taxon name | Number of species from Georgia reported within the study/known by the end of the study | ||||
---|---|---|---|---|---|
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|
|
|
Current study | |
8 | 14/16 | 16/16 | 16/16 | 45/47 | |
Suborder Psocomorpha | |||||
Amphipsocidae | |||||
Kolbia quisquiliarum | + | ||||
Caeciliusidae | |||||
Caecilius fuscopterus | + | ||||
Valenzuela atricornis | + | ||||
Valenzuela burmeisteri | + | + | + | + | |
Valenzuela flavidus | + | + | + | + | |
Valenzuela piceus | + | + | + | + | |
Ectopsocidae | |||||
Ectopsocopsis cryptomeriae | + | ||||
Ectopsocus briggsi | + | + | + | + | + |
Ectopsocus meridionalis | + | ||||
Ectopsocus vishnyakovae | + | ||||
Elipsocidae | |||||
Elipsocus hyalinus | + | ||||
Elipsocus moebiusi | + | ||||
Hemineura hispanica | + | ||||
Epipsocidae | |||||
Bertkauia lucifuga | + | ||||
Lachesillidae | |||||
Lachesilla bernardi | + | ||||
Lachesilla pedicularia | + | ||||
Lachesilla quercus | + | + | + | ||
Mesopsocidae | |||||
Mesopsocus laticeps | + | ||||
Mesopsocus unipunctatus | + | + | + | ||
Peripsocidae | |||||
Peripsocus alboguttatus | + | + | + | + | |
Peripsocus didymus | + | ||||
Peripsocus golubae | + | ||||
Peripsocus phaeopterus | + | + | + | + | |
Peripsocus subfasciatus | + | + | + | + | + |
Philotarsidae | |||||
Aaroniella badonneli | + | + | + | + | + |
Philotarsus picicornis | + | + | + | + | |
Psocidae | |||||
Amphigerontia contaminata | + | + | + | + | |
Loensia fasciata | + | ||||
Loensia variegata | + | ||||
Neopsocus rhenanus | + | ||||
Metylophorus nebulosus | + | ||||
Psococerastis gibbosa | + | ||||
Psocus bipunctatus | + | ||||
Trichadenotecnum alexanderae | + | ||||
Trichadenotecnum sexpunctatum | + | ||||
Stenopsocidae | |||||
Enderleinella obsoleta | + | ||||
Graphopsocus cruciatus | + | + | + | + | |
Stenopsocus immaculatus | + | + | + | + | |
Trichopsocidae | |||||
Trichopsocus dalii | + | + | + | + | + |
Suborder Troctomorpha | |||||
Liposcelididae | |||||
Embidopsocus enderleini | + | ||||
Liposcelis decolor | + | + | |||
Liposcelis meridionalis | + | ||||
Liposcelis rufa | + | ||||
Suborder Trogiomorpha | |||||
Psyllipsocidae | |||||
Dorypteryx domestica | + | ||||
Psyllipsocus ramburii | + | ||||
Trogiidae | |||||
Cerobasis guestfalica | + | ||||
Lepinotus reticulatus | + |
In the present contribution, we provide the results of recent countrywide investigations and significantly improve the existing knowledge on the psocid diversity of Georgia.
The main part of the studied material was collected within the framework of Caucasus Barcode of Life (CaBOL) project, being the most ambitious arthropod inventories ever performed in Georgia by the members of the GGBC (Georgian-German Biodiversity Center) and the CaBOL team of the Institute of Ecology, Ilia State University (https://ggbc.eu/). Most of the samples were collected using 18 Malaise traps installed in various habitats of the Kintrishi National Park from 20 April to 3 November 2018 at altitudes ranging from 403 m up to 2465 m (
Collected specimens were preserved in 96% ethanol, stored in a freezer at -22 °C at the scientific collections of Ilia State University. Unless otherwise stated, all material has been determined by the corresponding author, using a Zeiss Stemi Stereo Microscope with 8:1 Zoom and a Zeiss Apo 1.5× FWD 53 mm front lens attached and keys, provided by
Photographs of preserved specimens were taken using a Canon EOS 60D camera with a Canon MP-E 65mm f/2.8 1–5× Macro Photo Lens mounted on a Novoflex Castel-L Focusing Rack. Digital images were prepared using Zerene Stacker image stacking software and Adobe Photoshop CS6.
DNA was extracted from whole samples using the Quick-DNA Magbead Plus Kit (Zymo Research). Partial sequences of cytochrome oxidase subunit I (COI) were amplified by polymerase chain reaction (PCR) using the primer pair LCOI490-JJ and HCO2198-JJ (
In total 2353 specimens were collected representing 45 species comprising 15 families (Fig.
Suborder Psocomorpha Badonnel, 1951
Family *Amphipsocidae Pearman, 1936
Note. Representatives of the family have not been previously known to occur in Georgia. One species has been recorded within the current study.
* K. quisquiliarum Bertkau, 1882
Fig.
Material examined. 91 ♂♂.
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. Georgia.
Global distribution. European part of Western Palaearctic (
Genetics. We obtained eleven barcodes from the specimens with CaBOL-IDs 1027555, 1027556, 1027557 (BOLD:AFC5937), 1027571, 1027572, 1012880, 1012881, 1012882, 1012883, 1012884 and 1012885 (BOLD:AFC5936) (maximum p-distance 1.52%) with the nearest neighbor in BOLD systems being K. quisquiliarum from Finland (BOLD:ACG2800, mean p-distance 7.2%). Examined specimens perfectly matched the description provided by
Remarks. The species is characterized by micropterous females.
Family Caeciliusidae Kolbe, 1880
Note. Five species are recorded from Georgia.
* C. fuscopterus (Latreille, 1799)
Fig.
Material examined. 74 ♀♀, 60 ♂♂.
Distribution in Georgia. Kintrishi National Park, Lagodekhi National Park, Shilda.
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. Palaearctic (
Genetics. Eleven barcodes were obtained from the specimens with CaBOL-IDs 1012650, 1012720, 1012721, 1012722, 1012893, 1012895, 1012896, 1027603, 1027607, 1027611, 1027613 (BOLD:AEJ9302, maximum p-distance 1.56%) originating from two populations, with the nearest neighbor in BOLD systems being C. fuscopterus from Finland (BOLD:ACG3628, mean p-distance 5.43%). Examined specimens perfectly matched the description provided by
* V. atricornis (McLachlan, 1869)
Material examined. 42 ♀♀, 64 ♂♂.
Distribution in Georgia. Churia, Kintrishi National Park, Meore Moidanakhe, Shilda.
Distribution in Caucasus. Georgia.
Global distribution. Holarctic (
Genetics. We obtained three identical barcodes from the specimens with CaBOL-IDs 1026997, 1026998 and 1033176 (BOLD:ACG3154) that were identical to V. atricornis from Finland (BOLD:ACG3154).
Remarks. Originally V. atricornis is a species with Palaearctic distribution, introduced to North America from Hungary (
V. burmeisteri (Brauer, 1876)
Material examined. 20 ♀♀, 9 ♂♂.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. Holarctic (
Genetics. A single barcode was obtained from the specimen with CaBOL-ID 1027647 (BOLD:AEX9052) with the nearest neighbor in BOLD systems being V. burmeisteri from Germany (BOLD:AEX9052, p-distance 0.46%).
V. flavidus (Stephens, 1836)
Material examined. 152 ♀♀.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. Holarctic (
Genetics. Nine barcodes were obtained from specimens with CaBOL-IDs 1012664, 1012665, 1012666, 1012667, 1012668, 1012694, 1012695, 1026989, 1026990 (BOLD:AAN8447, maximum p-distance 0.46%), with the nearest neighbor in BOLD systems being V. flavidus from Canada (BOLD:AAN8447, mean p-distance 0.12%).
Remarks. The species is known to have asexual parthenogenetic populations in Europe, consisting of triploid females, but is believed to have sexual and asexual populations in North America as well (de Moya 2022).
V. piceus (Kolbe, 1882)
Material examined. 1 ♀.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. European part of Western Palaearctic (
Family Ectopsocidae Roesler, 1940
Note. Four species are recorded from Georgia.
* E. cryptomeriae (Enderlein, 1907)
Material examined. 258 ♀♀, 168 ♂♂.
Distribution in Georgia. Chkhorotsku, Dedoplistskaro, Kintrishi National Park, Mandaeti, Shilda, Tbilisi.
Distribution in Caucasus. North Caucasus: Krasnodar Krai (
Global distribution. Cosmopolitan (
Genetics. We obtained eleven nearly identical barcodes from the specimens with CaBOL-IDs 1012684, 1012918, 1027620, 1027621, 1027623, 1027624, 1030883, 1030884, 1032266, 1032267, 1032270 (BOLD:AAN8449, maximum p-distance 0.31%) with the nearest neighbor in BOLD systems being E. cryptomeriae from Canada (BOLD:AAN8449, maximum p-distance 0.16%).
Remarks. Ectopsocopsis cryptomeriae is a species of an Asian origin (
E. briggsi McLachlan, 1899
Fig.
Material examined. 156 ♀♀, 175 ♂♂.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Armenia (
Global distribution. Cosmopolitan (
Genetics. We obtained six barcodes from the specimens with CaBOL-IDs 1012678, 1026993, 1027564, 1027565, 1027609, 1032269 (BOLD:AAN8452, maximum p-distance 0.76%) identical to COI of E. briggsi from United States and Canada (BOLD:AAN8452) in BOLD systems.
* E. meridionalis Ribaga, 1904
Material examined. 11 ♀♀.
Distribution in Georgia. Patara Dmanisi, Tbilisi.
Global distribution. Subcosmopolitan - has not been recorded from Australia (
Genetics. We obtained six barcodes from the specimens with CaBOL-IDs 1027569, 1027570 (BOLD:ADB3092), 1032232, 1032233, 1032234, 1032235 (BOLD:AAM8931) (mean p-distance 2.53%) identical to COI of E. meridionalis from Costa Rica, Germany (BOLD:ADB3092) and Canada (BOLD:AAM8931) in BOLD systems.
Remarks. Cryptogenic species of unknown origin (
* E. vishnyakovae Schmidt, 1993
Material examined. 69 ♀♀ (macropterous), 1 ♀ (brachypterous).
Distribution in Georgia. Dighomi village, Tbilisi.
Distribution in Caucasus. Armenia (
Global distribution. Armenia, Turkmenistan (
Genetics. We obtained six identical barcodes from the specimens with CaBOL-IDs 1027578, 1027579, 1027580, 1027625, 1027642, 1027643 (BOLD:AEJ8025). There are no barcodes of the species available in BOLD systems as we submit the first ones.
Remark. This is by far the northernmost record of the species after it was reported from Armenia (Yerevan Botanical Garden) as E. brunneus Vishnyakova, 1963 (
Family *Elipsocidae Kolbe, 1882
Note. The representatives of the family have not previously been known to occur in Georgia. Three species have been recorded within the current study.
* E. hyalinus (Stephens, 1836)
Material examined. 17 ♀♀.
Distribution in Georgia. Kintrishi National Park, Mukhura, Tbilisi.
Distribution in Caucasus. Georgia.
Global distribution. Palaearctic (
Genetics. We obtained a single barcode from the specimen with CaBOL-ID 1032265 (BOLD:AFD9323). There are no barcodes of the species available in BOLD systems as we submit the first one.
* E. moebiusi Tetens, 1891
Material examined. 12 ♀♀, 12 ♂♂.
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. Western Palaearctic; Canada (
Genetics. We obtained nine barcodes from the specimens with CaBOL-IDs 1027594, 1027595, 1027596, 1027598, 1027626, 1027627, 1027628, 1027629, 1027630 (BOLD:AFD0492, maximum p-distance 0.46%) with the nearest neighbor in BOLD systems being E. moebiusi from Canada (BOLD:ACK6397, mean p-distance 4.26%).
* H. hispanica (Enderlein, 1907)
Material examined. 1 ♀.
Distribution in Georgia. Pia (Samtskhe-Javakheti).
Distribution in Caucasus. Armenia (
Global distribution. South Caucasus; Southern Europe (
Genetics. We obtained a single barcode from the specimen with CaBOL-ID 1020306 (BOLD:AEV8540). There are no barcodes of the species available in BOLD systems as we submit the first one.
Family *Epipsocidae Karny, 1930
Note. The representatives of the family have not previously been known to occur in Georgia. One species has been recorded within the current study.
* B. lucifuga (Rambur, 1842)
Material examined. 2 ♀♀.
Distribution in Georgia. Tekhuri River gorge (Martvili).
Distribution in Caucasus. Georgia.
Global distribution. Western Palaearctic (
Remarks. Species with mainly parthenogenetic reproduction and apterous females. Only few reports of males are known (
Family Lachesillidae Karny, 1930
Note. Three species are recorded from Georgia.
* L. bernardi Badonnel, 1938
Material examined. 38 ♂♂.
Distribution in Georgia. Mandaeti, Shilda, Tbilisi.
Distribution in Caucasus. Georgia.
Global distribution. Western Palaearctic (
Genetics. There are no barcodes of the species available in BOLD systems as we submit the first ones. We obtained seven nearly identical barcodes from the specimen with CaBOL-IDs 1012906, 1027584, 1027586, 1027587, 1027588, 1027591, 1027592 (BOLD:ACA3100, maximum p-distance 0.46%).
* L. pedicularia (Linnaeus, 1758)
Fig.
Material examined. 30 ♀♀, 32 ♂♂.
Distribution in Georgia. Kintrishi National Park, Mandaeti, Mukhura, Shilda, Tbilisi.
Distribution in Caucasus. Armenia (
Global distribution. Cosmopolitan (
Genetics. We obtained twelve barcodes from the specimens with CaBOL-IDs 1012698, 1012910, 1012911, 1027583, 1027585, 1027615, 1027616, 1027617, 1027645, 1032262, 1032263, 1032264 (BOLD:AAF1729, maximum p-distance 0.91%) with the nearest neighbor in BOLD systems being L. pedicularia from Canada (BOLD:AAN8449, maximum p-distance 0.46%).
L. quercus (Kolbe, 1880)
Fig.
Material examined. 26 ♀♀, 64 ♂♂.
Distribution in Georgia.
Distribution in Caucasus. Armenia (
Global distribution. Trans-Palaearctic (
Genetics. We obtained six barcodes from the specimens with CaBOL-IDs 1027568, 1027599, 1027600, 1027601, 1027602, 1027619 (BOLD:ADD4145, maximum p-distance 1.06%) with the nearest neighbor in BOLD systems being L. quercus from Finland (BOLD:ADD4145, mean p-distance 0.86%).
Family Mesopsocidae Enderlein, 1901
Note. Two species are recorded from Georgia.
* M. laticeps (Kolbe, 1880)
Material examined. 1 ♂.
Distribution in Georgia. Gori.
Distribution in Caucasus. Georgia.
Global distribution. Holarctic (
M. unipunctatus Kolbe, 1880
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Armenia (
Global distribution. Holarctic (
Family Peripsocidae Roesler, 1944
Note. Four species are recorded from Georgia.
P. alboguttatus (Dalman, 1823)
Fig.
Material examined. 5 ♀♀, 7 ♂♂.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Holarctic (
Genetics. Five nearly identical barcodes were obtained from specimens with CaBOL-IDs 1012699, 1012700, 1012701, 1012702 and 1012891 (BOLD:ACN1571, maximum p-distance 0.46%), with the nearest neighbor in BOLD systems P. alboguttatus from Bulgaria (BOLD:AAF3894, maximum p-distance 0.46%).
* P. didymus Roesler, 1939
Material examined. 2 ♀♀, 5 ♂♂.
Distribution in Georgia. Churia, Kintrishi National Park.
Distribution in Caucasus. Georgia.
Global distribution. Palaearctic (
* P. golubae Lienhard, 2006
Material examined. 3 ♀♀, 1 ♂.
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. Georgia.
Global distribution. Caucasus (
Genetics. Four identical barcodes from the specimens with CaBOL-IDs 1027573, 1027574, 1027593, 1027612 (BOLD:AEJ8129) are the first ones of the species that we submit in BOLD systems.
Remarks. Described from southern Russia (Krasnodar Krai) this is the first time the species is reported from South Caucasus, extending its known range south by 190 km.
P. phaeopterus (Stephens, 1836)
Material examined. 3 ♀♀, 2 ♂♂.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Armenia (
Global distribution. Holarctic (
P. subfasciatus (Rambur, 1842)
Material examined. 62 ♀♀.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Holarctic (
Genetics. Sixteen barcodes were obtained from specimens with CaBOL-IDs 1012659, 1012660, 1012661, 1012662, 1012877, 1026994, 1030871, 1030872, 1030873, 1030874, 1030875, 1030876, 1030877, 1030879, 1012723, 1012724 (BOLD:AAN8450), all identical to each other and to COI of P. subfasciatus from Germany (BOLD:AAN8450).
Family Philotarsidae Pearman, 1936
Note. Two species are recorded from Georgia.
A. badonneli (Danka, 1950)
Fig.
Material examined. 22 ♀♀.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Trans-Palaearctic (
Genetics. We obtained five nearly identical barcodes from the specimens with CaBOL-IDs 1027632, 1027633, 1027635, 1027636, 1027697 (BOLD:AAN8445, maximum p-distance 0.15%) with the nearest neighbor in BOLD systems being Aaroniella sp. from United States (BOLD:AAN8445, maximum p-distance 0.15%).
Remarks. Aaroniella badonneli is the only psocid species originally described from Georgia (
P. picicornis (Fabricius, 1793)
Material examined. 1 ♀.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Holarctic (
Genetics. We obtained a single barcode from the specimen with CaBOL-ID 1027558 (BOLD:ACB7137) identical to the COI of the P. picicornis from Canada (BOLD:ACB7137) in BOLD systems.
Family Psocidae Roesler, 1940
Note. Nine species are recorded from Georgia.
A. contaminata (Stephens, 1836)
Material examined. 16 ♀♀, 19 ♂♂.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Holarctic (
Genetics. We obtained nine barcodes from the specimens with CaBOL-IDs 1027554, 1027575, 1027576, 1027577, 1027631, 1033252, 1033253, 1033254 (BOLD:ACN1512, mean p-distance 0.89%) with the nearest neighbor in BOLD systems being A. contaminata from Germany with a private status (mean p-distance 1.14%).
* L. fasciata (Fabricius, 1787)
Material examined. 1♀, 1♂, 2 nymphs
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. Georgia.
Global distribution. Holarctic (
Genetics. We obtained two barcodes from the specimens with CaBOL-IDs 1012705 and 1012878 (BOLD:ACP8983, p-distance 1.27%) with the nearest neighbor in BOLD systems being L. fasciata from Norway (BOLD:ACP8983, maximum p-distance 1.27%).
* L. variegata (Fabricius, 1787)
Material examined. 2 nymphs
Distribution in Georgia. Lebarde.
Distribution in Caucasus. Karachay-Cherkessia (
Global distribution. Palaearctic (
Genetics. We obtained two identical barcodes from the specimens with CaBOL-IDs 1027655 and 1027656 (BOLD:ACN0757) with the nearest neighbor in BOLD systems being L. variegata from Finland (BOLD:ACN0757, mean p-distance 0.61%).
* N. rhenanus Kolbe, 1882
Material examined. 3 ♀♀ (brachypterous), 3 ♂♂.
Distribution in Georgia. Gori, Vardzia.
Distribution in Caucasus. Georgia.
Global distribution. European part of Western Palaearctic (
Genetics. The three identical barcodes obtained from the specimens with CaBOL-IDs 1028013, 1028014, 1028015 (BOLD:AEV8540) are the first ones of the species submitted in BOLD systems.
Remarks. The species is characterized by brachypterous (short-winged) females and winged males.
* M. nebulosus (Stephens, 1836)
Material examined. 10 ♀♀, 3 ♂♂.
Distribution in Georgia. Chkhorotsku, Churia, Kintrishi National Park.
Distribution in Caucasus. North Caucasus: Karachay-Cherkessia (
Global distribution. Palaearctic (
Genetics. We obtained seven barcodes from the specimens with CaBOL-IDs 1012683, 1012875, 1030867, 1030868, 1033177, 1033178, 1033180 (BOLD:ADA4803, maximum p-distance 0.61%) with the nearest neighbor in BOLD systems being Metylophorus sp. from Russia (BOLD:ADA4803, mean p-distance 0.31%).
* P. gibbosa (Sulzer, 1776)
Material examined. 10 ♀♀, 18 ♂♂.
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. North Caucasus: Karachay-Cherkessia (
Global distribution. Palaearctic (
Genetics. Six barcodes were obtained from specimens with CaBOL-IDs 1012680, 1012681, 1012682, 1012733, 1012862 and 1012876 (BOLD:ACC5474). The newly obtained barcodes were nearly identical (maximum p-distance 0.15%) with the nearest neighbor in BOLD systems being P. gibbosa from Finland (BOLD:ACC5474, maximum p-distance 0.15%).
* P. bipunctatus (Linnaeus, 1761)
Material examined. 3 ♂♂.
Distribution in Georgia. Kintrishi National Park, Mukhura.
Distribution in Caucasus. Georgia.
Global distribution. Palaearctic (
Genetics. We obtained two barcodes from the specimens with CaBOL-IDs 1032272, 1032273 (BOLD:AFC1275, p-distance 0.76%) with the nearest neighbor in BOLD systems being P. bipunctatus from Finland (BOLD:ADD9199, mean p-distance 5.81%).
* T. alexanderae Sommerman, 1948
Material examined. 6 ♀♀, 2 ♂♂.
Distribution in Georgia. Kintrishi National Park, Torsa.
Distribution in Caucasus. Georgia.
Global distribution. Nearctic (
Genetics. Four barcodes were obtained from specimens with CaBOL-IDs 1012703, 1012704, 1012837 and 1012838 (BOLD:ACE6318). The newly obtained barcodes were identical to each other as well as the nearest neighbor in BOLD systems T. alexanderae complex from Canada (BOLD:ACE6318).
Remarks. The only species from T. alexanderae complex known to occur in the Western Palaearctic is T. innuptum Betz, 1983 (
* T. sexpunctatum (Linnaeus, 1768)
Fig.
Material examined. 5 ♀♀, 4 ♂♂.
Distribution in Georgia. Telovani.
Distribution in Caucasus. North Caucasus: Karachay-Cherkessia (
Global distribution. Palaearctic (
Genetics. Seven identical barcodes were obtained from specimens with CaBOL-IDs 1027295, 1027296, 1027298, 1027299, 1027300, 1027301 and 1027307 (BOLD:AFC4535) with the nearest neighbor in BOLD systems T. sexpunctatum from Finland with a private status (p-distance 1.53%).
Family Stenopsocidae Kolbe, 1880
Note. Three species are recorded from Georgia.
* E. obsoleta (Stephens, 1836)
Material examined. 2 ♀♀, 8 ♂♂.
Distribution in Georgia. Kintrishi National Park.
Distribution in Caucasus. Georgia.
Global distribution. European part of Western Palaearctic (
Genetics. Two nearly identical barcodes were obtained from the specimens with CaBOL-IDs 1012706, 1027608 (BOLD:ACC7281, p-distance 0.15%) were identical to COI of E. obsoleta from Germany (BOLD:ACC7281).
G. cruciatus (Linnaeus, 1768)
Fig.
Material examined. 42 ♀♀, 212 ♂♂.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Armenia (
Global distribution. Holarctic (
Genetics. We obtained eight barcodes from the specimens with CaBOL-IDs 1012644, 1012645, 1012646, 1012647, 1012899, 1026995, 1030869, 1030880 (BOLD:ACA2933, mean p-distance 0.7%). The nearest neighbors in BOLD systems are as follows: 1012644, 1012645 (BOLD:ACA2933) to COI of G. cruciatus from Georgia (BOLD:AAF3894, similarity % = 100; 100 respectively); 1012646 (BOLD:ACA2933) to COI of G. cruciatus from Canada (BOLD:ACA2933, p-distance 0.31%); 1012647 (BOLD:ACA2933) to COI of G. cruciatus from Belarus (BOLD:AAF3894, p-distance 99.84); 1012899, 1026995, 1030869, 1030880 (BOLD:ACA2933) to COI of G. cruciatus from Norway (BOLD:ACA2933, similarity % = 98.31).
S. immaculatus (Stephens, 1836)
Material examined. 87 ♀♀, 60 ♂♂.
Distribution in Georgia. Batumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Palaearctic (
Genetics. Eight nearly identical barcodes were obtained from specimens with CaBOL-IDs 1012654, 1012655, 1012656, 1012657, 1012658, 1012725, 1012726 and 1012732 (BOLD:ABA6547, maximum p-distance 0.16%) with the nearest neighbor in BOLD systems being S. immaculatus from Finland (BOLD:ABA6547, mean p-distance 0.44%).
Family Trichopsocidae Pearman, 1936
Note. One species is recorded from Georgia.
T. dalii (McLachlan, 1867)
Material examined. 38 ♀♀, 74 ♂♂.
Distribution in Georgia. Sokhumi Botanical Garden (
Distribution in Caucasus. Georgia (
Global distribution. Holarctic (
Genetics. We obtained six identical barcodes from the specimens with CaBOL-IDs 1027561, 1027562, 1027563, 1027567, 1027589, 1027590 (BOLD:AAP2620) with the nearest neighbor in BOLD systems being T. dalii from Canada (BOLD:AAP2620, mean p-distance 0.16%).
Suborder Troctomorpha Roesler, 1940
Family Liposcelididae Enderlein, 1911
Note. Four species are recorded from Georgia.
* E. enderleini (Ribaga, 1905)
Material examined. 1 ♀.
Distribution in Georgia. Shilda.
Distribution in Caucasus. Georgia.
Global distribution. Argentina; Austria; Bahrein; Belgium; France; Great Britain; Italy; Madeira; South Africa (
Remarks. Described from Italy under the name of Stenotroctes enderleini (Ribaga 1905), E. enderleini belongs to the genus, which is most diversified in the South American-African region (
* L. rufa Broadhead, 1950
Material examined. 7 specimens.
Distribution in Georgia. Tbilisi (in balcony crevices).
Distribution in Caucasus. Georgia.
Global distribution. Cosmopolitan.
Genetics. We obtained three virtually identical barcodes from the specimens with CaBOL-IDs 1027271, 1027272 and 1027275 (BOLD:ACW0584). The newly obtained barcodes were identical to the nearest neighbor in BOLD systems L. rufa from unknown place of origin (BOLD:ACW0584) mined from GenBank.
Remarks. This is a species with originally a Mediterranean native range (
* L. meridionalis (Rosen, 1911)
Material examined. 28 specimens.
Distribution in Georgia. Pia, Tbilisi, Vardzia.
Distribution in Caucasus. Armenia (
Global distribution. Western Palaearctic (
Genetics. We obtained five nearly identical barcodes from the specimens with CaBOL-IDs 1030806, 1030807, 1030808, 1030810 (BOLD:AFC9720), 1030815 (BOLD:AFB9104) (maximum p-distance 0.76%). There are no barcodes of the species available in BOLD systems as we submit the first ones.
Remarks. Species with bisexual reproduction mainly occurring in the Mediterranean region, living in the litter, under the bark of trees, sometimes under stones (
Suborder Trogiomoropha Roesler, 1940
Family *Psyllipsocidae Kolbe, 1884
Note. The representatives of the family have not been previously known to occur in Georgia. Two species have been recorded within the current study.
* D. domestica (Smithers, 1958)
Material examined. 1 ♀.
Distribution in Georgia. Batsara Strict Nature Reserve.
Distribution in Caucasus. Georgia.
Global distribution. Western Palaearctic; Zimbabwe (
Genetics. A single barcode obtained from the specimen with CaBOL-ID 1027292 (BOLD:ACV6564) was identical to the nearest neighbor in BOLD systems D. domestica from Canada, France, and United States (BOLD:ACV6564).
Remarks. Dorypteryx domestica originates from Africa (
* P. ramburii Selys-Longchamps, 1872
Material examined. 2 ♀♀, micropterous.
Distribution in Georgia. Gori.
Distribution in Caucasus. Armenia (
Global distribution. Cosmopolitan (
Remarks. Cryptogenic species with unknown native range (
Family *Trogiidae Enderlein, 1911
Note. The representatives of the family have not been previously known to occur in Georgia. Two species have been recorded within the current study.
* C. guestfalica (Kolbe, 1880)
Material examined. 4 ♀♀.
Distribution in Georgia. Grigoleti, Qvabiskhevi.
Distribution in Caucasus. Georgia.
Global distribution. Cosmopolitan (
Remarks. Cerobasis guestfalica generally reproduces via parthenogenesis. Males are known from Great Britain and Poland (
* L. reticulatus (Linnaeus, 1768)
Material examined. 25 ♀♀.
Distribution in Georgia. Dighomi village, Gori, Pia, Tbilisi, Vardzia.
Distribution in Caucasus. Armenia (
Global distribution. Cosmopolitan (
Genetics. We obtained five nearly identical barcodes from the specimens with CaBOL-IDs 1030801, 1030802, 1030803, 1030804, 1030805 (BOLD:AFC5414, maximum p-distance 0.61%) with the nearest neighbor in BOLD systems being L. reticulatus from Canada (BOLD:ADD4959, mean p-distance 1.57%).
Remarks. Cryptogenic species of unknown origin (
Danka laid a profound basis with her research on the Psocoptera fauna of Georgia, even though it was limited to only a few locations almost exclusively in Batumi and Sokhumi Gardens located at the Black Sea coast. The material collected and examined in our study mostly also originates from the western part of Georgia (see Suppl. material
Census on Psocoptera of Georgia. 1Classification according
Taxon name1 | Species reported from Georgia | Species reported from ex-USSR Western Palaearctic European part ( |
Species reported from adjacent countries | Estimated species unrecorded from Georgia | ||||
---|---|---|---|---|---|---|---|---|
|
Current study | Karachaevo-Cherkessia ( |
Armenia ( |
Azerbaijan ( |
Turkey ( |
|||
Suborder Psocomorpha | ||||||||
Amphipsocidae | ||||||||
Kolbia quisquiliarum | + | + | ||||||
Caeciliusidae | ||||||||
Caecilius fuscopterus | + | + | + | + | ||||
Valenzuela labinae | + | |||||||
Valenzuela atricornis | + | + | ||||||
Valenzuela burmeisteri | + | + | + | + | ||||
Valenzuela corsicus | + | |||||||
Valenzuela despaxi | + | + | + | |||||
Valenzuela flavidus | + | + | + | + | + | + | ||
Valenzuela gynapterus | + | |||||||
Valenzuela piceus | + | + | + | + | ||||
Ectopsocidae | ||||||||
Ectopsocopsis cryptomeriae | + | + | ||||||
Ectopsocus briggsi | + | + | + | + | ||||
Ectopsocus meridionalis | + | |||||||
Ectopsocus spec. | + | |||||||
Ectopsocus vishnyakovae | + | + | + | |||||
Ectopsocopsis xerophylla | + | |||||||
Elipsocidae | ||||||||
Cuneopalpus cyanops | + | |||||||
Elipsocus abdominalis | + | |||||||
Elipsocus hyalinus | + | + | ||||||
Elipsocus pumilis | + | |||||||
Elipsocus moebiusi | + | + | + | + | ||||
Hemineura dispar | + | + | + | |||||
Hemineura hispanica | + | + | + | |||||
Pseudopsocus fusciceps | + | |||||||
Reuterella helvimacula | + | + | + | |||||
Epipsocidae | ||||||||
Bertkauia lucifuga | + | + | + | + | ||||
Lachesillidae | ||||||||
Lachesilla spec. | + | |||||||
Lachesilla bernardi | + | + | ||||||
Lachesilla dimorpha | + | |||||||
Lachesilla pedicularia | + | + | + | |||||
Lachesilla quercus | + | + | + | + | + | + | ||
Lachesilla rossica | + | |||||||
Lachesilla tanaidana | + | |||||||
Mesopsocidae | ||||||||
Mesopsocus immunis | + | |||||||
Mesopsocus laticeps | + | + | ||||||
Mesopsocus unipunctatus | + | + | + | + | ||||
Mesopsocus vernus | + | |||||||
Peripsocidae | ||||||||
Peripsocus alboguttatus | + | + | + | + | + | |||
Peripsocus didymus | + | + | ||||||
Peripsocus golubae | + | + | ||||||
Peripsocus parvulus | + | + | + | + | ||||
Peripsocus phaeopterus | + | + | + | + | + | |||
Peripsocus subfasciatus | + | + | + | + | ||||
Philotarsidae | ||||||||
Aaroniella badonneli | + | + | + | |||||
Philotarsus picicornis | + | + | + | |||||
Psocidae | ||||||||
Amphigerontia bifasciata | + | + | + | |||||
Amphigerontia contaminata | + | + | + | |||||
Amphigerontia intermedia | + | |||||||
Blaste conspurcata | + | + | + | + | ||||
Blaste quadrimaculata | + | |||||||
Hyalopsocus contrarius | + | + | + | |||||
Hyalopsocus morio | + | |||||||
Loensia fasciata | + | + | ||||||
Loensia pearmani | + | |||||||
Loensia variegata | + | + | + | |||||
Metylophorus nebulosus | + | + | + | |||||
Neopsocopsis hirticornis | + | |||||||
Neopsocus rhenanus | + | + | + | |||||
Psocidus flavonimbatus | + | |||||||
Psococerastis gibbosa | + | + | + | |||||
Psocus bipunctatus | + | + | ||||||
Trichadenotecnum alexanderae | + | |||||||
Trichadenotecnum germanicum | + | |||||||
Trichadenotecnum majus | + | + | + | |||||
Trichadenotecnum sexpunctatum | + | + | + | |||||
Stenopsocidae | ||||||||
Enderleinella obsoleta | + | + | ||||||
Graphopsocus cruciatus | + | + | + | + | + | + | ||
Stenopsocus immaculatus | + | + | + | |||||
Stenopsocus lachlani | + | + | + | |||||
Stenopsocus stigmaticus | + | |||||||
Trichopsocidae | ||||||||
Trichopsocus clarus | + | |||||||
Trichopsocus dalii | + | + | + | |||||
Suborder Troctomorpha | ||||||||
Liposcelididae | ||||||||
Embidopsocus enderleini | + | |||||||
Liposcelis bostrychophila | + | + | ||||||
Liposcelis brunnea | + | |||||||
Liposcelis decolor | + | + | ||||||
Liposcelis divinatoria | + | + | + | |||||
Liposcelis entomophila | + | |||||||
Liposcelis formicaria | + | |||||||
Liposcelis meridionalis | + | + | + | |||||
Liposcelis silvarum | + | + | + | |||||
Liposcelis rufa | + | |||||||
Liposcelis tricolor | + | |||||||
Protroctopsocidae | ||||||||
Reticulopsocus besucheti | + | |||||||
Suborder Trogiomorpha | ||||||||
Prionoglarididae | + | |||||||
Prionoglaris kapralovi | + | |||||||
Prionoglaris stygia | + | |||||||
Psyllipsocidae | ||||||||
Dorypteryx domestica | + | |||||||
Psyllipsocus ramburii | + | + | + | |||||
Trogiidae | ||||||||
Cerobasis annulata | + | |||||||
Cerbosas guestfalica | + | |||||||
Lepinotus inquilinus | + | |||||||
Lepinotus reticulatus | + | + | + | + | + | |||
Trogium pulsatorium | + |
This study, originally conceived as a small addition to the fauna of Georgian psocopterans, eventually turned into a large-scale exciting journey through the study of a new group of arthropods for the corresponding author. In the end, we hope that our study inspires the readership to spend more time in nature observing and unveiling the hidden gems, reminding them that science begins with subsiding the mind’s curiosity.
The responsibility for the content of this publication lies with the author. Deepest gratitude to the CaBOL project team members for their help with sorting the material and Nino Kachlishvili, Ekaterine Gabashvili, and Anastasia Datunashvili from the genetic laboratory of Ilia State University, Institute of Ecology for their sequencing work. Much obliged to Kazunori Yoshizawa and Charles Lienhard for their valuable comments that helped to improve the quality of the manuscript, and the Agency of Protected Areas for giving us the collection permit #655-0-2-202103182033. Special thanks to Gintautas Steiblys for his help in finding the missing literature.
No conflict of interest was declared.
No ethical statement was reported.
The project on which this study is based was funded by the Federal Ministry of Education and Research under grant number 01DK20014A.
Armen Seropian has written the text and determined the specimens; Eka Arsenashvili, Natalia Bulbulashvili, Anano Shubashishvili, Giorgi Iankoshvili, Mariami Todua, Ana Ananiashvili, Shota Japarashvili, Tinatin Chkhartisvhili, Aleksi Memishishi, Sopio Balkhamishvili, Beka Chitadze, Elisabeth Karalashvili, Levan Mumladze, Nils Hein, and Björn Rulik contributed to the material collecting and further sorting; Giorgi Iankoshvili generated the sampling map in GIZ; Levan Mumladze and Nils Hein improved the very first draft of the Manuscript.
Armen Seropian https://orcid.org/0000-0003-3777-9954
Eka Arsenashvili https://orcid.org/0000-0002-7330-9602
Natalia Bulbulashvili https://orcid.org/0000-0002-6802-1209
Anano Shubashishvili https://orcid.org/0000-0002-9747-2703
Giorgi Iankoshvili https://orcid.org/0000-0002-0429-1488
Mariami Todua https://orcid.org/0000-0003-1870-3030
Ana Ananiashvili https://orcid.org/0000-0002-4186-0723
Shota Japarashvili https://orcid.org/0000-0002-9674-3719
Tinatin Chkhartisvhili https://orcid.org/0000-0002-0429-1488
Aleksi Memishishi https://orcid.org/0000-0002-5862-5105
Sopio Balkhamishvili https://orcid.org/0000-0001-5198-305X
Beka Chitadze https://orcid.org/0000-0001-7311-5827
Elisabeth Karalashvili https://orcid.org/0000-0002-9015-7604
Levan Mumladze https://orcid.org/0000-0002-2172-6973
Nils Hein https://orcid.org/0000-0002-5172-8531
Björn Rulik https://orcid.org/0000-0002-2808-9305
All of the data that support the findings of this study are available in the main text or Supplementary Information.
Details for sampling locations
Data type: excel file