Research Article |
Corresponding author: Weihai Li ( lwh7969@163.com ) Academic editor: Sven Bradler
© 2023 Mengyu Li, Bingli Wang, Ding Yang, Dávid Murányi, Weihai Li, Hongliang Wang.
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Li M, Wang B, Yang D, Murányi D, Li W, Wang H (2023) New and little-known stonefly species of the Rhopalopsole vietnamica ‘western assemblage’ group (Plecoptera, Leuctridae) from China. ZooKeys 1183: 125-137. https://doi.org/10.3897/zookeys.1183.103288
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We examined Chinese stonefly specimens of the Rhopalopsole vietnamica ‘western assemblage’ group. A new species from Hainan Province, R. bawanglinga Li, Li & Yang, sp. nov. is described and illustrated from male and female adults, and it is compared to closely related taxa. The hitherto unknown female of R. hainana Li & Yang, 2010 is described. Morphological evidence is presented for the identity of R. dentiloba Wu, 1973, on the basis of topotypes from Yunnan Province, southwestern China.
female description, new species, redescription, Rhopalopsole bawanglinga, Rhopalopsole dentiloba, Rhopalopsole hainana, taxonomy
The stonefly genus Rhopalopsole Klapálek, 1912 is one of the largest leuctrid genera, with about one hundred valid species, distributed in the Oriental and East Palaearctic regions (
World list of species belonging to the Rhopalopsole vietnamica ‘western assemblage’ group. Abbreviation: M, male; F, female.
Species | Known life stages | Distribution |
---|---|---|
R. amamiensis Kawai, 1967 | M, F | Japan: Kagoshima Prefecture, Okinawa |
R. assamensis Sivec & Harper, 2008 | M, F | India: Assam |
R. bawanglinga sp. nov. | M, F | China: Hainan |
R. brevidigitata Qian & Du, 2017 | M | China: Yunnan |
R. dentiloba (Wu, 1973) | M, F | China: Yunnan |
R. fengyangshanensis Yang, Shi & Li, 2009 | M | China: Fujian, Zhejiang |
R. furcospina (Wu, 1973) | M, F | China: Guangxi, Shaanxi, Sichuan, Zhejiang |
R. hainana Li & Yang, 2010 | M | China: Hainan |
R. nanlinga Yang & Du, 2022 | M | China: Guangdong |
R. sinensis Yang & Yang, 1993 | M | China: Chongqing, Fujian, Gansu, Guangxi, Guizhou, Hubei, Ningxia, Shaanxi, Zhejinag; Vietnam: Laocai |
R. singiplatta Yang & Du, 2022 | M, F | China: Sichuan |
R. sipirokana Sivec & Harper, 2008 | M, F | Sipirok: Sumatra |
R. vietnamica Sivec & Harper, 2008 | M, F | Vietnam: Thinhoa |
R. yajunae Li & Yang, 2010 | M | China: Zhejiang |
R. yunnana Sivec & Harper, 2008 | M, F | China: Yunnan |
China, Hainan Province specimens used for COI based identification, genetic differentiation, and circumscription analyses of three Rhopalopsole species.
The specimens were collected by hand and preserved in 75% ethanol. Most of the studied materials are deposited in the
Entomological Museum of China Agricultural University, Beijing, China (
The maps were downloaded from Standard Map Service (http://bzdt.ch.mnr.gov.cn/) and edited by Photoshop 2020, map number is GS (2019)1823.
Total genomic DNA was extracted from thorax muscle using the TIANamp Genomic DNA kit (Tiangen Biotechnology, Beijing, China) following the manufacturer’s protocol. The universal primers LCO1490 and HCO2198 (
Family Nemouridae Billberg, 1820
Genus Rhopalopsol e Klapálek, 1912
Holotype
: male (
Male adult of this species is characterized by the lateral projections of the tergum 10 being bifurcate with the upper spine longer than the lower one in lateral view. The cercus bears a stout dorsoapical spine, and the epiproct is subtrapezoid with deep, circularly incised anterior margin in dorsal view. Females are diagnosed by the posterior margin of sternum 7 forming a narrowly produced pregenital plate, posteromedial portion slightly bilobed.
Adult habitus. Forewing length is 6.8 mm in the male, 7.0–7.4 mm in the females. Head brown to dark brown, slightly wider than pronotum; compound eyes black; antennae and mouthparts brownish to brown. Pronotum brown with dark rugosities; wings subhyaline with darker veins; legs brown. Abdomen brown.
Male (Figs
Female (Fig.
The specific name refers to the Bawangling National Forest Park.
China (Hainan).
The new species is a typical member of the R. vietnamica group, western assemblage sensu
Rhopalopsole hainana Li & Yang, 2010: 59 (original description).
11 males, 5 females (
Female. (Fig.
Genetic distance refers to the genetic divergence between species and can be used to compare the genetic similarity between difference species (
Genetic distances among sequenced specimens. Upper (right) triangular matrix: standard deviation; lower (left) triangular matrix: genetic distances.
J61 F R. hainana | J64 F R. hainana | J66 M R. hainana | |
---|---|---|---|
J61 F R. hainana | 0.006 | 0.0 | |
J64 F R. hainana | 0.009 | 0.006 | |
J66 M R. hainana | 0.0 | 0.009 |
We compared the holotype with the specimens from Baisha County, which originated from near the type locality. The hitherto unknown female resembles the female of R. bawanglinga, but the tip of the pregenital plate is not bilobed, and the transverse band on sternum 8 is more pronounced.
Rhopalopsole dentiloba Wu, 1973: 105 (original description).
4 males, 1 female (
We examined several specimens from Xishuangbanna, Yunnan Province, which is the same location where R. dentiloba was described. The original description and illustrations did not provide sufficient details for identification. Therefore, we discuss herein the distinctive morphological characters of our specimens. This species is a typical member of the R. vietnamica group, western assemblage sensu
Thirteen species were previously recorded in the R. vietnamica ‘western assemblage’ group, as specified in Table
The specimens used in this study were partially provided by National Animal Collection Resource Center, Beijing, China.
The authors have declared that no competing interests exist.
No ethical statement was reported.
We acknowledge the support of the National Natural Science Foundation of China (No. 31000977) and the Program for Science and Technology Innovation Talents in Universities of Henan (No. 21HASTIT042).
Ding Yang, Dávid Murányi and Weihai Li: Review, commentary or revision; Bingli Wang and Hongliang Wang: Color illustrations acquisition; Mengyu Li: Manuscript writing.
Mengyu Li https://orcid.org/0000-0002-7950-4142
Bingli Wang https://orcid.org/0000-0002-1080-549X
Ding Yang https://orcid.org/0000-0002-7685-3478
Dávid Murányi https://orcid.org/0000-0002-3907-5590
Hongliang Wang https://orcid.org/0000-0002-9758-1328
All of the data that support the findings of this study are available in the main text.