Maximum likelihood phylogram of Centrolene. The analysis is based on 2457 base pairs of concatenated mitochondrial DNA from 12S and 16S, and nuclear DNA from POMC gene fragments. Outgroup is not shown; the tree was routed with Allophryne ruthveni. In red, the new species and in green a sequence newly generated by the present study. The catalog number, species name, country, and in the case of Ecuadorian species province and short locality names, are shown next to each terminal (associated data are listed in Appendix 1).