UCM 32263 Phrynosoma solare imaged with one shot and 20 stacked images. A the single shot was photographed using a narrow aperture (f/13) to maximize depth of field, however, extremities and other regions with changes in depth (such as nose and tail) are out of focus under 100% magnification. Z-stacking resolves these issues. Both images are high quality and suitable for a wide range of applications, however B the photo generated by focus stacking is more technically sound for research applications that require fine morphological detail.