Phylogenetic reconstruction of Alainites species and allied taxa, based on maximum likelihood analysis of sequences of the mitochondrial COI gene. The reconstruction includes representatives of the two new Alainites species (displayed with orange background), all other sequenced Alainites species (blue), and representative species of Nigrobaetis and Takobia (grey). For full details of selected samples see Suppl. material 1. ML bootstrap values higher than 50% are indicated next to the nodes; lower than 50% are collapsed. Due to the wide polytomy in deeper nodes, branch lengths are in fact meaningless in this presentation. Taxa names are consistent with our findings, and may sometimes differ from names entered in GenBank.