Nine new species of the spider genus Pireneitega Kishida, 1955 (Agelenidae, Coelotinae) from Xinjiang, China

Abstract Nine new Pireneitega species collected from Xinjiang, China are described as new to science: Pireneitega burqinensis sp. n. (♂♀), Pireneitega fuyunensis sp. n. (♂♀), Pireneitega gongliuensis sp. n. (♂♀), Pireneitega huochengensis sp. n. (♂♀), Pireneitega lini sp. n. (♀), Pireneitega liui sp. n. (♂♀), Pireneitega wensuensis sp. n. (♂), Pireneitega wui sp. n. (♂) and Pireneitega yaoi sp. n. (♀). DNA barcodes were obtained for all these species for future use.


Introduction
The spider genus Pireneitega was established by Kishida (1955). Its type species is Amaurobius roscidus C.L. Koch, 1843 from Germany, considered to be a junior synonym of P. segestriformis (Dufour, 1820). Pireneitega was for a long time regarded as a nomen nudum until Wang and Jäger (2007) found reasons to revalidate this name and to make Paracoelotes Brignoli, 1982 its junior synonym. Currently, there are twenty-one valid Pireneitega species, distributed widely from the Iberian Peninsula to Japan and Sakhalin; eleven of them are known from East Asia, six are known from Central Asia, and other four from Europe Lin 2015, World Spider Catalog 2016). This paper provides descriptions of nine new Pireneitega species collected from Xinjiang in northwestern China.

Material and methods
Specimens were examined with a Leica M205C stereomicroscope. Images were captured with an Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on an Olympus SZX12 dissecting microscope. Epigynes and male palps were examined after dissection from the spiders' bodies. The epigyne was cleared by boiling it in a 10% KOH solution before taking photos of the vulva.
All measurements were obtained using a Leica M205C stereomicroscope and are given in millimeters. Leg measurements are given as: Total length (femur, patella + tibia, metatarsus, tarsus). Only structures (palp and legs) of the left side of the body are described and measured. The terminology used in the text and the figure legends follows Wang (2002). Abbreviations used in this paper and in the figure legends are: A = epigynal atrium; ALE = anterior lateral eye; AME = anterior median eye; AME-ALE = distance between AME and ALE; AME-AME = distance between AME and AME; ALE-PLE = distance between ALE and PLE; CD = copulatory duct; CF = cymbial furrow; CO = conductor; E = embolus; EB = embolic base; ET = epigynal tooth; FD = fertilization duct; H = epigynal hood; MA = median apophysis; PA = patellar apophysis; PLE = posterior lateral eye; PME = posterior median eye; PME-PLE = distance between PME and PLE; PME-PME = distance between PME and PME; R = receptacle; RTA = retroventral tibial apophysis; ST = subtegulum; T = tegulum; TC = tip of conductor.
DNA barcodes were obtained for future use. A partial fragment of the mitochondrial gene cytochrome oxidase subunit I (COI) was amplified and sequenced for nine new species and one old species using Primers LCO1490-oono (5'-CWACAAAYCA-TARRGATATTGG-3') (Folmer et al. 1994;Miller et al. 2010) and HCO2198-zz (5'-TAAACTTCCAGGTGACCAAAAAATCA-3') (Folmer et al. 1994;Chen et al. 2015). For additional information on extraction, amplification, and sequencing procedures, see Zhao et al. 2013. All sequences were deposited in GenBank and the accession numbers are provided in Table 1.
All of the specimens (including molecular vouchers) are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing, China.  6A-B); other coelotines usually have a small epigynal atrium, the short epigynal teeth and narrow copulatory ducts. The males of this genus can be distinguished from other coelotines by with an elongated and flattened conductor which is usually twisted into a circle horizontally or vertically and a large median apophysis ( Fig. 1A-C); other coelotines usually have a broad or short conductor and a reduced or indistinct median apophysis. Description. Described in Wang (2002, sub Paracoelotes).
Distribution. Known only from the type locality ( Fig. 17). Etymology. The specific name refers to the type locality; adjective. Diagnosis. The male can be distinguished from all other Pireneitega species, except P. burqinensis sp. n. and P. tianchiensis, by having a hook-shaped conductor, and can be distinguished from these two species by the small and narrow median apophysis (the broad and nearly fins-shaped apophysis in P. burqinensis sp. n. and P. tianchiensis) (cf. Figs 1, 3 and 12; Wang et al.1990: figs 81-83). The female can be distinguished from all other Pireneitega species, except P. burqinensis sp. n. and P. tianchiensis, by having short receptacles and the large epigynal atrium, and can be distinguished from these two species by the receptacles, about 1.5 times as long as wide (about two times longer than wide in P. burqinensis sp. n. and about 1.2 times in P. tianchiensis) (cf. Figs 2A-B, 4A-B and 13A-B; Wang et al. 1990: figs 84-85).
Distribution. Known only from the type locality (Fig. 17). Etymology. The specific name refers to the type locality; adjective. Diagnosis. The male can be distinguished from all other Pireneitega species, except P. involuta and P. xinping, by having a broad conductor and thick patellar apophysis, and can be distinguished from these two species by the tapering tip of conductor (the rounded tip of conductor in P. involuta and P. xinping) (cf. Fig.  5; Wang et al. 1990: figs 13-15;Zhang et al. 2002: figs 9-10). The female can be distinguished from all other Pireneitega species, except for P. xinping, by having large copulatory ducts, and can be distinguished from this species by the short and thick epigynal teeth, about 0.5 times as long as epigynal atrium (the long and narrow epigynal teeth in P. xinping, subequal to the length of epigynal atrium) (cf. Fig.  6A-B; Zhang et al.2002: figs 7-8).
Distribution. Known only from the type locality (Fig. 17). Diagnosis. The female can be distinguished from all other Pireneitega species, except for P. luniformis, by having spiral receptacles, and can be distinguished from this species by the narrow and straight epigynal teeth (the broad and bent epigynal teeth in P. luniformis) (cf. Fig. 9A-B; Zhu and Wang 1994: figs 5-6).
Distribution. Known only from the type locality (Fig. 17). Etymology. The specific name is a patronym in honor of the collector Jincheng Liu; noun (name) in genitive case.

Pireneitega liui
Diagnosis. The male can be easily distinguished from all the other Pireneitega species, except P. luniformis, by having a long and narrow conductor, and can be distinguished from this species by the blunt tip of the patellar apophysis (the tapering tip of conductor and the patellar apophysis in P. luniformis) (cf. Fig. 10; Zhu and Wang 1994: figs 7-8). The female can be distinguished from all other Pireneitega species, except P. major (Kroneberg, 1875) by having the nearly trapezoidal epigynal atrium, and can be distinguished from this species by the abrupt tip of epigynal teeth (the pointed tip of teeth in P. major) (cf. Fig. 11A-B; Kroneberg 1875: fig. 6).

Pireneitega wensuensis
Distribution. Known only from the type locality (Fig. 17).  Diagnosis. The male can be distinguished from all other Pireneitega species, except P. armeniaca by having bended and narrow conductor, and can be distinguished from this species by the blunt tip of median apophysis (the tapering tip of median apophysis in P. armeniaca) (cf. Fig. 15; Brignoli 1978: figs 117-121).
Distribution. Known only from the type locality (Fig. 17). Etymology. The specific name is a patronym in honor of the collector Zhiyuan Yao; noun (name) in genitive case.

Pireneitega yaoi
Diagnosis. The female can be distinguished from all other Pireneitega species, except P. burqinensis sp. n., P. fuyunensis sp. n. and P. tianchiensis, by having the weakly sclerotized tip of septum. It can be distinguished from these three species by the nearly rectangular epigynal atrium (while P. burqinensis sp. n. has a nearly triangular atrium; and P. tianchiensis and P. fuyunensis sp. n., large and nearly square-shaped atrium) (cf. Figs 2A-B, 4A-B, 6A-B and 16A-B; Wang et al. 1990: figs 84-85).
Distribution. Known only from the type locality (Fig. 17).