Biological model and bacterial cultures

Experimental design

Priming procedure and LD50 injection

Haemocyte analysis

β-galactosidase activity

Statistical analysis

Survival abilities

Body weight of females

First reproductive event

Second reproductive event

Total number of offspring

Haemocyte parameters and senescence biomarkers

Immune priming improves the survival abilities but negatively impacts the mean body weight of females

No evidence of immune priming costs on the reproductive abilities of double infected females

Indirect effect of immune priming on the first clutch production through the body weight of females

Heavier females have reduced probability to produce a second clutch, regardless of the number of infections

While immune priming neither impacts the haemocyte parameters nor the senescence patterns after reproduction, producing two clutches increases the cellular senescence of females