2 urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7E urn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727A ZooKeys ZK 1313-2989 1313-2970 Pensoft Publishers 10.3897/zookeys.1052.64607 64607 Catalogue Amphibia Animalia Reptilia Natural History Systematics Zoology & Animal Biology Africa Asia Saving collections: taxonomic revision of the herpetological collection of the Instituto de Investigação Científica Tropical, Lisbon (Portugal) with a protocol to rescue abandoned collections Ceríaco Luis M. P. luisceriaco@gmail.com https://orcid.org/0000-0002-0591-9978 1 2 Conceptualization Data curation Formal analysis Investigation Methodology Project administration Supervision Validation Writing - original draft Writing - review and editing Parrinha Diogo 2 Formal analysis Investigation Writing - original draft Writing - review and editing Marques Mariana P. mptlmarques@gmail.com https://orcid.org/0000-0002-1712-2632 2 3 Conceptualization Data curation Formal analysis Investigation Methodology Project administration Supervision Validation Writing - original draft Writing - review and editing Museu de História Natural e da Ciência da Universidade do Porto, Praça Gomes Teixeira 4099-002 Porto, Portugal Universidade de Lisboa Lisboa Portugal Departamento de Zoologia e Antropologia (Museu Bocage), Museu Nacional de História Natural e da Ciência, Universidade de Lisboa, Rua da Escola Politécnica, 58, 1269-102 Lisboa, Portugal Museu de História Natural e da Ciência da Universidade do Porto Porto Portugal Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), InBIO, Universidade do Porto, Rua Padre Armando Quintas 7, Vairão, 4485-661 Porto, Portugal Universidade do Porto Porto Portugal

Corresponding authors: Luis M. P. Ceríaco (luisceriaco@gmail.com); Mariana P. Marques (mptlmarques@gmail.com)

Academic editor: Angelica Crottini

 2021 30 07 2021 1052 83 156 C3CAE445-16F5-53A5-BFEC-BE1446618435 2A386AE0-607D-4847-AA45-CDF771B101E2 5168166 18 02 2021 03 06 2021 Luis M. P. Ceríaco, Diogo Parrinha, Mariana P. Marques This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://zoobank.org/2A386AE0-607D-4847-AA45-CDF771B101E2

The herpetological collections of the Instituto de Investigação Científica Tropical, Lisbon, are amongst the most important collections from the former Portuguese territories in Africa and Asia. The collection comprises more than 5000 preserved specimens, including type specimens of nine taxa, Trachylepis adamastor, Trachypelis thomensis, Panaspis thomensis, Naja peroescobari, Dalophia angolensis, Hemidactylus nzingae, Boaedon fradei, Platysaurus maculatus maculatus, and Platysaurus maculatus lineicauda. The collection was abandoned in the early years of 2000s and was at risk of being lost. In this paper the entire collection is reviewed, a catalogue provided of the extant specimens, and a brief account of the history of herpetological research at IICT given. Details are also provided on the recovery of the collection and a protocol to rescue abandoned collections.

 Angola Cabo Verde East Timor Goa Guinea-Bissau herpetofauna Macau Mozambique Natural History Collections São Tomé & Príncipe Fundação para a Ciência e a Tecnologia 501100001871 http://doi.org/10.13039/501100001871 JRS Biodiversity Foundation 100012428 http://doi.org/10.13039/100012428 Citation

Ceríaco LMP, Parrinha D, Marques MP (2021) Saving collections: taxonomic revision of the herpetological collection of the Instituto de Investigação Científica Tropical, Lisbon (Portugal) with a protocol to rescue abandoned collections. ZooKeys 1052: 85–156. https://doi.org/10.3897/zookeys.1052.64607

 Introduction

The zoological collections of the Instituto de Investigação Científica Tropical (IICT), Lisbon (Portugal), are amongst the largest and most important biological collections in Portugal. Spanning all major zoological groups, the IICT collections are mostly focused on the fauna of the former Portuguese colonial territories in Africa and Asia. The collection’s geographical coverage is of interest, as they cover areas from where collections are relatively scarce and countries for which the current faunal knowledge is still deficient. While part of the IICT zoological collections have been catalogued and digitised recently (see Monteiro et al. 2014, 2016, 2017), a considerable part was neglected for years. This was the case of the herpetological collections. With approximately 5000 specimens from Angola, Mozambique, Guinea-Bissau, Cabo Verde, São Tomé & Príncipe, East Timor, Macau (China), Goa (Portuguese India), and Portugal, the IICT herpetological collections are the largest of their kind in the country. These collections are also amongst the largest available collections of amphibians and reptiles in the world for some of the countries covered (e.g., Guinea-Bissau, Cabo Verde). These collections were built from the 1930s to the 1990s, during several expeditions and field surveys conducted by IICT researchers and staff, but also by donations and contributions from several Portuguese colonial officers and landowners in these former territories.

Despite its importance, the IICT herpetological collections were abandoned in the late 1990s. Without a fully dedicated curator or collection manager, the collection became degraded and almost completely inaccessible for researchers. Besides the original field books and an incomplete manuscript catalogue, the collections were never fully catalogued or digitised. In 2015 we started a cataloguing and digitisation project for the IICT herpetological collections. This process was followed by a complete evaluation of the conservation status of the collections, the recovery of the specimens that were in critical condition, and the transfer of the entire collection to the Museu Nacional de História Natural e da Ciência, Lisbon. In this paper we provide a comprehensive review of the taxonomic diversity and importance of the IICT herpetological collections, report on the collection recuperation process, and propose a protocol to recover abandoned natural history collections.

 Brief history of the <abbrev content-type="institution" xlink:title="Instituto de Investigação Científica Tropical, Lisboa, Portugal" id="ABBRID0ELCAC">IICT</abbrev> and its herpetological collections

Dating back to the second half of the nineteenth century, IICT’s institutional history has been particularly complex, even in the bureaucracy-prone context of Portuguese scientific institutions. Its origins are related to the foundation of the ‘Comissão de Cartografia’ in 1883, but only in 1936 did the institution become more active, when it was renamed ‘Junta das Missões Geográficas e de Investigações Coloniais’. This would not be the last name change of the institution, as it would be renamed ‘Junta de Investigações do Ultramar’ in 1945, ‘Junta de Investigações Científicas do Ultramar’ in 1973, ‘Laboratório Nacional de Investigação Científica Tropical’ in 1979, and finally ‘Instituto de Investigação Científica Tropical’ in 1982 (Anonymous 1983). The reform of the institution in 1936 and the publication of the National Plan for the Scientific Occupation of the Portuguese Overseas in 1945 (Anonymous 1945) established the internal organisation of the institution, namely its departments and branches. Subsequently, the institution established a centre for zoological research, commonly known as “Centro de Zoologia” [also known by the acronym CZL – Centro de Zoologia de Lisboa, see Sabaj (2020)]. The Centro de Zoologia was housed in an adapted mansion in Rua da Junqueira 14, Alcântara neighborhood, near the Tagus river, Lisbon. This centre was the main entity responsible for conducting zoological research in the Portuguese overseas territories, building, and maintaining collections and providing zoological expertise to other fields related to the colonial enterprise.

The leadership of the Centro de Zoologia was appointed to the Portuguese zoologist Fernando Frade Viegas da Costa (1898–1983), commonly known as Fernando Frade (Fig. 1). Besides being the director of the CZL, Frade also had the responsibility for leading most of the so-called “Missões Zoológicas” (Zoological Missions) to the Portuguese overseas territories. The objective of these missions was to catalogue the fauna of those territories, study their ecological relationships, and understand the potential use or threats that native fauna could pose to the colonial enterprise (Anonymous 1945). During Frade’s direction the CZL led several zoological missions, namely to Guinea-Bissau, São Tomé & Príncipe, Cabo Verde, Angola, Mozambique, Portuguese India (Goa) and East Timor. Herpetological specimens were collected in the course of all these missions, as well as on other missions, as was the case for the Apiary Mission to Angola (1957–1959).

10.3897/zookeys.1052.64607.figure1 5168168 24B3E710-E424-5576-B7D5-1A99FED487BE

Fernando Frade during field work in Guinea-Bissau. Photograph credits: ULisboa – Col.Fotografia IICT-ZOO 22149.

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The study of the herpetological collections was entrusted to the Portuguese herpetologist Sara Maria Bárbara Marques Manaças (1896–date of death unknown; Fig. 2). Manaças published 20 papers in which she identified and catalogued specimens, reported morphological data, and provided taxonomic and distribution comments. Although she worked with both amphibians and reptiles, the majority of her papers were focused on reptiles (7 vs. 13 publications, respectively). Her papers covered the herpetofauna of Guinea-Bissau (Manaças 1947, 1949, 1950a, 1951a, b, 1955, 1982), Mozambique (Manaças 1950b, 1952, 1954, 1959, 1961a, 1982), Portuguese Timor (currently East Timor) (Manaças 1956, 1972), São Tomé & Príncipe (Manaças 1958, 1973), Portuguese India – Goa (Manaças 1961b), and Angola (Manaças 1963, 1973 “1974”, 1982). All of these papers were single-authored by Manaças, with the exception of one, co-authored with Fernando Frade, on the venomous snakes of the Portuguese overseas territories (Frade and Manaças 1955; Fig. 3). The last paper of her career, also dedicated to the venomous snakes of Guinea-Bissau, São Tomé & Príncipe, Angola, and Mozambique (Manaças 1982), was published posthumously with some additions and corrections by the Portuguese herpetologist Margarida Pinheiro (date of birth unknown–to date). During the time Manaças curated the herpetological collections, the English-born Zimbabwean herpetologist Donald G. Broadley (1932–2016) visited and consulted the collections in August 1968 (Broadley 2018).

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Portrait of Sara Manaças (A), and her herpetological laboratory (date unknown; B). Photograph credits: A courtesy of Luis Mendes B ULisboa – Col.Fotografia IICT-ZOO 21688.

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Plates with the water colours of the Portuguese painter António Silva Lino (1911–1984) for the poorly known manuscript “Serpentes do Ultramar Português”, co-authored by Fernando Frade and Sara Manaças (Frade and Manaças 1955).

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Following the passing of Manaças in the early 1980s, Pinheiro was entrusted with the herpetological collections (Fig. 4). With the independence of the former Portuguese territories (with the exception of Macau) in 1975, fieldwork almost halted, and the activity of the herpetology department slowed down considerably. Pinheiro focused mostly on the study of Cabo Verde’s herpetofauna (Pinheiro 1986, 1990), but also participated in a field survey to the then Portuguese territory of Macau (currently a special administrative region of the People’s Republic of China; Dias et al. 1994). From this latter survey, Pinheiro published a brief note on the distribution of the Agamid Leiolepis belliana (Hardwicke & Gray, 1827) in the region (Pinheiro 1994) and participated as a co-author, together with the herpetologist Clara Ruas (date of birth unknown–to date) and the entomologist Luís Mendes (1946–to date), on two notes on the diet of local amphibians (Mendes et al. 1994a, b). During the 1990’s, Clara Ruas became assistant researcher in the herpetological department. Ruas focused almost exclusively on the study of amphibians, and besides the aforementioned publications, she published two papers regarding the amphibians of Angola (Ruas 1996, 2002a) and one on the amphibians of Mozambique (Ruas 2002b).

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Margarida Pinheiro working with herpetological specimens in one of the laboratories of the Centro de Zoologia in 1979. Photograph credits: ULisboa – Col.Fotografia IICT 21199.

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During the first decades of the twenty-first century, the Centro de Zoologia became increasingly understaffed and research in the collections diminished considerably. This contributed to the degradation of the herpetological collections. Without constant supervision, lacking proper collection management, and housed in two rooms in the basement of the Centro de Zoologia building, which suffered from the lack of climate control, high humidity levels, and no security, the herpetological collections reached a critical situation and were at risk of being irreplaceably lost (Fig. 5).

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Herpetological collections reserve rooms in the basement of Centro de Zoologia, IICT, Lisbon, in July 2014 A entrance view of the room dedicated to the reptile collections, note the broken door B another view of the room dedicated to the reptile collections C view of the room dedicated to the amphibian collections D close-up of the poor conservation conditions of preserved specimens. Photographs by Luis M. P. Ceríaco.

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After years of uncertainty regarding its future, IICT was formally closed by the Portuguese government on 31 July 2015 (Decreto-Lei 141/2015). With this closure, all the responsibilities of the institution, namely those of research and development, were transferred from the mansion in Rua da Junqueira to the University of Lisbon and its natural history collections were subsequently deposited in the Museu Nacional de História Natural e da Ciência (MUHNAC), Lisbon. The transfer of the collections from Centro de Zoologia to MUHNAC took place between 2015 and 2018. Despite sharing the same spaces and curatorial team, the IICT collections remain independent from the rest of MUHNAC collections, i.e., they maintain their former catalogue numbers and have not been catalogued as MUHNAC collections. The IICT herpetological collection is also considered a “closed” collection, in the sense that it is not accepting additional specimens, and therefore constitutes a closed set. In January 2018, the first author of this paper (LMPC) was appointed as External Curator of the IICT herpetological collections, while the third author (MPM) is the acting Assistant Curator.

 Materials and methods

During an initial survey, all the available catalogues, field notebooks, and documentation associated with the collections were located, compiled, and digitised. The available field notebooks (one per major expedition), provided locality data, collecting dates, names of the collectors and/or identification of the expedition, and an assortment of natural history data and observations (Fig. 6A). Contrary to the catalogue numbers, the numbers in the field notebooks (i.e., the field numbers) were the same as those physically associated to the specimen. This number, comprising a serial number and the collecting year (e.g., 161/1959) was the same number used in all of the publications referable to the specimen, and therefore should be considered the de facto catalogue number. Manuscript documentation associated with the collection provided measurements, scale counts, and observations made by Sara Manaças for individual specimens (Fig. 6B, C). The available catalogue was organised by subcollection, with one entry per specimen, providing their taxonomic identity, collecting date, and locality (Fig. 6D). Each specimen also had a catalogue number, but this number was not physically associated with the specimen, nor was the respective field number, rendering it impossible to link the entry for the catalogue number and the specimen.

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Documentation associated with the herpetological collection A field notebook of the Apiary Missions in Angola B data sheet associated with São Tomé snakes C data sheet associated with Angolan snakes D Manuscript catalogue of the herpetological collection. The “n° Registo” [record number] is not physically attached to the specimen, making it impossible to link the two. Photographs by Diogo Parrinha.

https://binary.pensoft.net/fig/572774 List of institute acronyms (abbreviations follow Sabaj 2020)

AMNHAmerican Museum of Natural History, New York, USA;

BPBMBernice Pauahi Bishop Museum, Department of Zoology, Honolulu;

CASCalifornia Academy of Sciences, San Francisco, USA;

CMCarnergie Museum, Pittsburgh, USA;

CZLCentro de Zoologia de Lisboa [extinct, now part of IICT, see below], Lisbon, Portugal;

DiSSCO Distribued System of Scientific Collections;

GBIF Global Biodiversity Information Facility;

iDigBio Integrated Digitized Biocollections;

IICT Instituto de Investigação Científica Tropical, Lisboa, Portugal;

INBAC Instituto Nacional da Biodiversidade e Áreas de Conservação, Luanda, Angola;

ISCED Instituto Superior de Ciências da Educação, Lubango, Angola;

MCNBMuseu de Ciències Naturals de Barcelona, Barcelona, Spain;

MCUCMuseu da Ciência da Universidade de Coimbra, Coimbra, Portugal;

MDMuseu Regional do Dundo, Dundo, Angola;

MCZMuseum of Comparative Zoology, Harvard University, Cambridge, USA;

MHNCMusée d’Histoire Naturelle, la Chaux-de-Fonds, Switzerland;

MHNC-UPMuseu de História Natural e da Ciência da Universidade do Porto, Porto, Portugal;

MNHNMuséum National d’Histoire Naturelle, Paris, France;

MUHNACMuseu Nacional de História Natural e da Ciência, Lisbon, Portugal;

MVZMuseum of Vertebrate Zoology, University of California, Berkeley, USA;

NCSMNorth Carolina Museum of Natural Sciences, Raleigh, USA;

NHMUKNatural History Museum, London, UK;

PEMPort Elizabeth Museum, Port Elizabeth, South Africa;

TCWC Biodiversity Research and Teaching Collections, Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, USA;

TMDitsong National Museum of Natural History, Pretoria, South Africa;

UMMZUniversity of Michigan Museum of Zoology, Ann Arbor, USA;

USNMSmithsonian Institution, National Museum of Natural History;

YPMYale University, Peabody Museum of Natural History, New Haven, USA;

ZFMKZoologisches Forschlungmuseum Alexander Koenig, Bonn, Germany;

ZSI Zoological Society of India, India.

All herpetological specimens were located in the CZL reserves, and after a brief initial identification and listing (Fig. 7A), were transported to the Wet Laboratory of MUHNAC. Specimens were distributed in different types of glass jars, with most of the specimens in glass-top, wire-bail jars with rubber gaskets, although some specimens were in glass jars with Bakelite or metal lids. None of the jars was in optimal condition, ranging from dirty to broken. Almost all the specimens were preserved in formaldehyde, as inferred based on the examination. Once in the laboratory, jars and other containers were cleaned in order to retrieve the available data from the existing external labels (Fig. 7B). These data were confirmed against the contents of the jar/container as well as with any existing specimen tags or internal labels (either attached to the specimens or in the bottom of the jar; Fig. 7C). Data on the jars and specimens were cross-referenced to data available in scientific publications that cited IICT specimens to confirm the presence of individual specimens in the collections and compare the published data to the data on the label/tags (Manaças 1947, 1949, 1950a, b, 1951a, b, 1952, 1954, 1955, 1956, 1958, 1959, 1961a, b, 1972, 1973, 1973 “1974”, 1982; Pinheiro 1986, 1990, 1994; Mendes et al. 1994a, b; Ruas 1996, 2002a, b; Ceríaco 2015; Ceríaco et al. 2016, 2017, 2020a, b, 2021; Soares et al. 2018; Hallermannn et al. 2020). All specimens, whenever possible, were identified to species level. For this we followed the most updated checklists, guides, and identification keys to the groups and geographical regions covered in the collection.

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Rehabilitation and revision of the IICT herpetological collections A initial listing of the specimens to be transported B cleaning of the external label C example of internal label, usually attached to individual specimens D general view of the different types of jars and respective conservation issues associated–embrittled and or melted rubber gaskets, oxidized metal stoppers, cracked jars, etc E new standardized ResistAll internal label FIICT herpetology collection after the intervention in the MUHNAC reserves. Photographs by Luis M. P. Ceríaco.

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As the majority of the jars presented structural deficiencies (cracks, melted or embrittled gaskets, rusty/oxidised metal stoppers and/or lids, etc.; Fig. 7D) they were discarded. Original external labels were retrieved whenever possible. Following a similar procedure to that suggested by Simmons (2014), specimens that were originally preserved in formaldehyde (the vast majority) were rinsed in distilled water for a few minutes, then underwent steps of 20% increase in concentration of ethanol (20% for 30 minutes, 40% for 30 minutes, 60% for 30 minutes, 70% for final preservation). All the specimens were placed in 70% ethanol. In those cases in which the original jar/container had been discarded, specimens were placed in new jars. Original tags and/or internal labels were kept inside the new jars and, whenever possible, original external labels were reattached to the exterior. A new standardised, typewritten label was printed on ResistAll paper and placed inside each jar, presenting the basic data its content (Fig. 7E). After this, specimens were deposited in MUHNAC’s Wet Collections reserves, placed in compactor cabinets, and arranged by country of origin (sub-collection) and then taxonomically (Fig. 7F).

Some specimens presented critical conservation issues such as being dehydrated due to evaporation of the preservative fluid (Fig. 8A), fungal and bacterial growth (Fig. 8B), deposits of formaldehyde crystals, loss of proteins and lipids (Fig. 8C), or decomposition of the specimens (Fig. 8D, E). For dehydrated specimens we attempted a slow rehydration through the placement of the specimen in a humid atmosphere and staging in water overnight. Specimens with fungal and bacterial growth were washed in 70% ethanol and the growth carefully removed with cotton swabs. Specimens with deposits of formaldehyde crystals were washed with water then a series of steps of 20% increase in concentration of ethanol (20% for 30 minutes, 40% for 30 minutes, 60% for 30 minutes, 70% for final preservation); the few specimens that showed loss of proteins and lipids underwent similar treatment. Specimens that were soft due to problems during the fixation process were injected and immersed with formaldehyde for one to two days, depending on the size of the specimen. Specimens that were beyond salvation were discarded, but each was photographed for archival purposes and their associated data was collected whenever possible.

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Examples of problems associated with the IICT herpetological collections A a dehydrated specimen of Gerrhosaurus multilineatus from Angola B a jar with extensive fungal growth on the inside and outside C lipid loss on a snake specimen from Macau D jar in which the preservative fluid has evaporated and the specimen was attacked by insect pests E rotten specimen of Leptopelis due to poor initial fixation and evaporation of the ethanol preservative. Photographs by Mariana P. Marques.

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All specimens with locality data were georeferenced. Locality data are reported in the form of decimal degrees and use the WGS 84 map datum. Older (non-GPS) records were georeferenced using the GEOLocate web application (https://www.geo-locate.org). Whenever possible original maps, field books or collectors’ notes were consulted. Elevations are all reported as meters above sea level.

 Results

The collections are divided into eight sub-collections, respectively the Cabo Verde collection, the Guinea-Bissau collection, the São Tomé & Príncipe collection, the Angola collection, the Mozambique collection, the Portuguese India collection, the Macau collection, and the East Timor collection. Some specimens from Portugal and other African countries also exist in the collections, but represent a diminutive subset, mostly originating from occasional collecting events by IICT researchers or external donations. Combined, the eight sub-collections hold a total of 5173 specimens (3048 reptiles, 2125 amphibians). The largest sub-collection is that of Cabo Verde (1740 specimens), followed by Mozambique (1181 specimens), Guinea-Bissau (980 specimens), Angola (677 specimens), São Tomé & Príncipe (234 specimens), Macau (318 specimens), Portuguese India – Goa (26 specimens), and East Timor (17 specimens). The specimens were collected in 253 different localities across their respective countries. The collections hold 47 type specimens (two holotypes, 45 paratypes) of nine nominal taxa. Surprisingly, only 76 jars with an unknown number of specimens (due to the poor preservation or total destruction of the specimens) were discarded because they were beyond salvation. Detailed reviews of the different sub-collections are provided in the following accounts.

Cabo Verde collection

The herpetofauna of the Cabo Verde archipelago has been reviewed in the past decades and is currently well known. The most recent account of its terrestrial reptiles was published by Vasconcelos et al. (2013), listing a total of 31 extant taxa, of which 22 are endemic to the archipelago. Seven exotic reptiles have been recorded in Cabo Verde (Vasconcelos et al. 2013; Ceríaco and Sousa 2017), and the only amphibian present in the archipelago is the exotic African Common Toad, Sclerophrys regularis (Reuss, 1833) (Vasconcelos et al. 2010b). These numbers are constantly being updated, as a new species has been recently described (Vasconcelos et al. 2020). The iconic giant skink, Chioninia coctei (Duméril & Bibron, 1839) is presumed to have gone extinct in the twentieth century due to a combination of human and ecological factors, and recent searches for this species have been unsuccessful (Ceríaco 2014).

This collection comprises a total of 1740 specimens (376 amphibians and 1364 reptiles), composed of one species of amphibians and 17 species and four subspecies of reptiles (Table 1). All the amphibians in the collection belong to the invasive African Common Toad (Sclerophys regularis), accounting for roughly 20% of the total collection. The reptile collection is composed of representatives from the families Gekkonidae, Phyllodactylidae and Scincidae, each represented by a single genus. Family Phyllodactylidae is the best represented with 871 specimens, followed by Scincidae with 489 and Gekkonidae with only four specimens. All specimens from the family Phyllodactylidae belong to endemic species of the genus Tarentola, and all species known to occur in Cabo Verde are represented in the collection, except Tarentola boavistensis Joger, 1993. Of the two recognised subspecies of Tarentola gigas (Bocage, 1875), only the nominal T. g. gigas is present in the collection, while both subspecies of Tarentola protogigas Joger, 1984 are represented (T. p. protogigas and T. p. hartogi). With 296 specimens, Tarentola nicolauensis Schleich, 1984 is the best represented species, followed by Tarentola substituta Joger, 1984, T. gigas gigas and Tarentola cf. caboverdiana Schleich, 1984 with 131, 128 and 124 specimens, respectively. The family Scincidae is represented by the endemic genus Chioninia, covering all known species except for the extinct C. coctei. At the subspecific level, C. vaillantii vaillantii (Boulenger, 1887) and C. spinalis maioensis (Mertens, 1955) are the only recognised subspecies that are represented in this collection. Within the Scincidae, C. delalandii (Duméril & Bibron, 1839) is the best represented species with 220 specimens, followed by C. nicolauensis (Schleich, 1987) with 90 specimens. The few specimens from the family Gekkonidae are assigned to Hemidactylus sp. and Hemidactylus angulatus Hallowell, 1854, which is the only exotic reptile from Cabo Verde that is represented in the collection.

Overview of the Cabo Verde amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
ANURA Duméril, 1806
Bufonidae (Gray, 1825) Sclerophrys Tschudi, 1838 Sclerophrys regularis (Reuss, 1833) Brava Island: Fajã D´Água – IICT/A 295–301/1969; Vinagre – IICT/A 273–294/1969; 376
Santiago Island: Assomada – IICT/A 41–42/1969, 59–66/1969, 170–196/1969, 272/1969; Engenho – IICT/A 43–53/1969, 67–85/1969, 222–224/1969, 231–255/1969, 261–263/1969, 268–271/1969; Boa Entrada – IICT/A 54–58/1969, 86–91/1969, 264–267/1969, 1–11/1972; 18–31/1972; Boa Entrada stream – IICT/A 12–14/1969; Chão de Tanque – IICT/A 27–34/1969; Mato Sancho stream [or Mato Sanches stream] – IICT/A 1–11/1969; Picos – IICT/A 227–230/1969; Picos stream – IICT/A 225–226/1969; Praia Formosa – IICT/A 1–2/1993; Santa Catarina – IICT/A 10/1969, 16–26/1969, 256–260/1969, 32–37/1972, CV1–9, CV11, CV13; São Jorge dos Orgãos – IICT/A 103–169/1969; Sedeguma – IICT/A 35–40/1969, 92–102/1969, 197–214/1969, 15–17/1972; Santiago Island [unknown locality] – IICT/A 12–15/1969; 215–221/1969, CV10;
São Nicolau Island: Ribeira João – IICT/A 4–21/1970; Ribeira Brava – IICT/A 1–3/1970
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 376
REPTILES
SQUAMATA Oppel, 1811
Gekkonidae Gray, 1825 Hemidactylus Goldfuss, 1820 Hemidactylus angulatus Hallowell, 1854 Santo Antão Island: Ponta do Sol – IICT/R 292/1972; Porto Novo – CV18 3
Fogo Island: São Filipe – IICT/R 442/1969
Hemidactylus sp. Santo Antão Island: Ponta do Sol, Fantanha – IICT/R CV16 1
Phyllodactylidae Gamble, Bauer, Greenbaum & Jackman, 2008 Tarentola Gray, 1825 Tarentola bocagei Vasconcelos, Perera, Geniez, Harris & Carranza, 2012 São Nicolau Island: between Jucalinho and Carrissal – IICT/R 509–515/1970; Carvoeiro – IICT/R 354–357/1970; Preguiça– IICT/R 167–174/1970; Preguiça Airfield – IICT/R 152–159/1970, 161–166/1970, 175–180/1970, 599–603/1970, 624–631/1970; Ribeira das Queimadas – IICT/R 37/1970; Ribeira Maiamba – IICT/R 135–140/1970; Taboleiro – IICT/R 580–586/1970; São Nicolau Island [unknown locality] – IICT/R 48/1970, 58–59/1970, 499–509/1970 61
Tarentola cf. caboverdiana Schleich, 1984 Santo Antão Island: Ponta do Sol – IICT/R 334–338/1972, CV15; Porto Novo – IICT/R 119–134/1972, 145–156/1972, 166–243/1972, 232–/1972; Santo Antão Island [unknown locality] – IICT/R 135–144/1972, 246–258/1969 124
Tarentola darwini Joger, 1984 Santiago Island: Praia – IICT/R CV19; S. João Batista – IICT/R 448–449/1969 2
Tarentola fogoensis Vasconcelos, Perera, Geniez, Harris & Carranza, 2012 Fogo Island: São Filipe – IICT/R 427–24871969, 435/1969, 439–441/1969, 444–446/1969; Fogo Island [unknown locality] – IICT/R 447/1969 10
Tarentola gigas gigas (Bocage, 1875) Raso Islet: Raso Islet [unknown locality] IICT/R 230–231/1970, 237/1970, 239–271/1970, 363/1970, 367–452/1969, 631–637/1970, CV3 128
Tarentola maioensis Schleich, 1984 Maio Island: Airport – IICT/R 365–375/1969; Barreiro – IICT/R 377–381/1969; Calheta – IICT/R 349/1969, 358/1969; Lagoa – IICT/R 343–348/1969; Vila de Maio – IICT/R 334–338/1969; Maio Island [unknown locality] – IICT/R 339–342/1969, 359–364/1969; 376/1969, 382–389/1969; 391–398/1969 61
Tarentola nicolauensis Schleich, 1984 São Nicolau Island: between Jucalinho and Carrissal – IICT/R 488–498/1970; between Ribeira Brava and Juncalinho– IICT/R 516–523/1970; Cabeçalinho – IICT/R 548–561/1970; Calejão – IICT/R 212–229/1970, 289–296/1970, 638–650/1970; 296
Phyllodactylidae Gamble, Bauer, Greenbaum & Jackman, 2008 Tarentola Gray, 1825 Tarentola nicolauensis Schleich, 1984 Carvoeiro – IICT/R 340–353/1970, 358–362/1970, 598–623/1970; Chanzinha – IICT/R 284–288/1970; Preguiça – IICT/R 141–151/1970; Perguiça port – IICT/R 297–315/1970; Ribeira da Prainha – IICT/R 187–211/1970; Ribeira de Caixa – IICT/R 316–319/1970; Ribeira Maiamba – IICT/R 126–134/1970, 181–186/1970; Ribeira Seca – IICT/R 320–334/1970; S. João – IICT/R 272–277/1970; S. Nicolau hostel – IICT/R 475/1970; Taboleiro – IICT/R 533–539/1970, 562–579/1970, 583A–585A/1970, 587–595/1970; Ribeira Brava – IICT/R 115–125/1970, 335–339/1970 ; Ribeira Brava surroundings – IICT/R 476–487/1970; São Nicolau Island [unknown locality] – IICT/R 596–597/1970
Tarentola protogigas hartogi Joger, 1993 Brava Island: Achada do Favatal – IICT/R 403–405/1969, 412–421/1969; Vinagre – IICT/R 422–425/1969 24
Rombos Islet: 406–411/1969, CV32
Tarentola protogigas protogigas Joger, 1984 Fogo Island: São Filipe – IICT/R 426/1969, 429/1969, 433–434/1969, 436–43871969; São Filipe vivarium – IICT/R 443/1969; Vale de Cavaleiros lighthouse – IICT/R 292/1969 9
Tarentola raziana Schleich, 1984 Branco Islet: Branco Islet [unknown locality] – IICT/R 470–474/1969 14
Raso Islet: Raso Islet [unknown locality] IICT/R 232–237/1970, 364–366/1970
Tarentola rudis Boulenger, 1906 Santiago Island: Praia – IICT/R 350–357/1969, 399/1969 10
Tarentola substituta Joger, 1984 São Vicente Island: Baia das Gatas – IICT/R 301–31871972; Calhau – IICT/R 321–333/1972; Mato Inglês – IICT/R 339–377/1972; Monte Sossego – IICT/R 396–412/1972; Ribeira Passarão – IICT/R 381–395/1972; São Pedro – IICT/R 259–287/1970; 131
Tarentola sp. Fogo Island: Fogo Island [unknown locality] – IICT/R 431/1969 1
Scincidae Cuvier, 1808 Chioninia Gray, 1845 Chioninia delalandii (Duméril & Bibron, 1839) Brava Island: Achada do Favatal – IICT/R 237–255/1969; Nova Sintra – IICT/R 213–216/1969, 221–236/1969, 271–276/1969, CV14; Nova Sintra graveyard – IICT/R 217–220/1969, 221A–223A/1969; Senhora do Monte – IICT/R 256–261/1969; Vinagre – IICT/R 264–270/1969; Brava Island [unknown locality] – IICT/R 193/1969, 207–212/1969 Pinheiro (1986, 1990) 220
Maio Island: Calheta – IICT/R 26–30/1969, 38–43/1969, 47/1969, 49/1969; Maio Island [unknown locality] – IICT/R 90/1969, 96–97/1969
Santiago Island: Assomada – IICT/R 158 Boa Entrada – IICT/R 159/1969, 161/1969, 171/1969; Boa Entrada – IICT/R 131–133/1969, 144–147/1969, 156–159/1969, 189–192/1969, 45–47/1972; Chão da Fazenda – IICT/R 24–26/1972; Praia – IICT/R 58–74/1969; Santa Catarina – IICT/R 27–32/1972, 53–72/1972, 79–93/1972; Tarrafal – IICT/R 176/1969; Santiago Island [unknown locality] – IICT/R 162–165/1969, 288–291/1969, 33–39/1972, CV29–30
Fogo Island: São Filipe – IICT/R 297–300/1969, 303/1969, 323–327/1969; São Filipe vivarium – IICT/R 328–331/1969; Fogo Island [unknown locality] – IICT/R 292–332/1969, CV2
Chioninia fogoensis (O’Shaughnessy, 1874) Santo Antão Island: Chão do Mocho – IICT/R 13–19/1972; Porto Novo – IICT/R 10–11/1972, 234–245/1972, 1001–1012/1972, CV17; Vale Paúl stream – IICT/R 4–9/1972, 298–300/1972; Santo Antão Island [unknown locality] – IICT/R 1–3/1972, 12/1972, 158–165/1972, 379–380/1972 Pinheiro (1990) 56
Chioninia nicolauensis (Schleich, 1987) São Nicolau Island: Caleijão – IICT/R 79–85/1970; Preguiça Airfield – IICT/R 12–13/1970, 160/1970; Ribeira das Queimadas – IICT/R 41–47/1970, 49–57/1970; Ribeira João – IICT/R 1–11/1970; São Nicolau Island [unknown locality] – IICT/R 14–40/1970, 60–78/1970, 102–106/1970, 651/1970 Pinheiro (1990) 90
Scincidae Cuvier, 1808 Chioninia Gray, 1845 Chioninia spinalis maioensis (Mertens, 1955) Maio Island: Alcatraz – IICT/R 98–108/1969; Bumba stream – IICT/R 9–14/1969; Calheta – IICT/R 26A–37A/1969; 44–46/1969, 48/1969, 50/1969, 52–56/1969; Maio Island [unknown locality] – IICT/R 1–8/1969, 15–19/1969, 109–110/1969; Lage-Branca Islet – IICT/R 75–83/1969, CV3 Pinheiro (1990) 64
Chioninia strangeri (Gray, 1845) Raso Islet: Raso Islet [unknown locality] IICT/R 86–114/1970 Pinheiro (1990) 19
Chioninia vaillantii vaillantii (Boulenger, 1887) Santiago Island: Assomada – IICT/R 166–170/1969, 180–188/1969; Boa Entrada – IICT/R 118–120/1967, 125–130/1967, 148–150/1969, 172–175/1969, 1340–1343/1969; Tarrafal – IICT/R 177–178/1969; Santiago Island [unknown locality] – IICT/R 121/1969, 138–142/1969, 151–153/1969, 44/1972, CV28 Pinheiro (1986) 40
TOTAL NUMBER OF REPTILES SPECIMENS 1364

The geographic range of this collection covers 74 different localities from ten islands and islets: São Nicolau, Santiago, Brava, Santo Antão, Maio, Raso, Fogo, São Vicente, Rombos, and Branco (Table 2; Fig. 9). Of all the major islands of the archipelago, only three are not represented: Santa Luzia in the Desertas group, and the eastern islands of Sal and Boavista. São Nicolau is the best represented island, with 474 specimens from 24 different localities, while the Rombos islets are the least sampled with only seven specimens. Collecting events took place between 1967 and 1994, although most of the material was collected between 1969 and 1972 in expeditions organised by the CZL, especially aimed at collecting birds and invertebrates.

Gazetteer of Cabo Verde localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Island/Islet Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Brava Achada do Favatal [or Ach do Favatal] Achada do Favatal (currently a waste dump) 14.877435, -24.686155 1616 308 2/18
Brava Achada do Ferreiro Achada do Ferreiro 14.837713, -24.727839 1376 238 1/11
Brava Braga Brava Island [or Brava] 14.852066, -24.700687 5640 905 1/6
Brava Cemitério de Nova Sintra Nova Sintra graveyard 14.876142, -24.69713 125 478 1/7
Brava Fajã D´Água Fajã D´Água 14.870898, -24.730602 500 39 1/7
Brava Nova Sintra Nova Sintra 14.870483, -24.695545 747 497 2/30
Brava Senhora do Monte Senhora do Monte 14.857583, -24.719033 500 640 1/6
Brava Vinagre Vinagre 14.866667, -24.7 350 634 3/33
Brava Ilha Brava Brava Island 14.852066, -24.700687 5640 905 1/1
Maio Aeroporto Airport 15.156885, -23.214155 1659 11 1/11
Maio Alcatraz Alcatraz 15.217212, -23.103711 206 24 1/11
Maio Barreiro Barreiro 15.135554, -23.160517 370 31 1/5
Maio Calheta Calheta 15.229421, -23.210159 800 9 3/37
Maio Ilhéu Lage-Branca Lage-Branca Islet 15.313, -23.137 47 – 1/10
Maio Lagoa Lagoa 15.132533, -23.133345 540 3 1/6
Maio Vila de Maio Vila de Maio 15.138703, -23.211588 730 22 1/5
Maio Ribeira de Bumba Bumba stream [undetermined locality] – – – 1/6
Maio Ilha de Maio Maio Island 15.216667, -23.166667 13613 82 2/61
Santiago Assomada Assomada, Santa Catarina 15.098508, -23.67269 3036 542 1/40
Santiago Boa Entrada Boa Entrada, Santa Catarina 15.125985, -23.674844 420 475 4/77
Santiago Ribeira Boa Entrada Boa Entrada stream 15.125498, -23.675343 1017 475 1/3
Santiago Chã da Fazenda Sta. Catarina Chão da Fazenda, Santa Catarina 15.083664, -23.669757 5513 371 1/2
Santiago Santa Catarina, Chão de Tanque Chão de Tanque, Santa Catarina 15.094417, -23.705235 506 237 1/8
Santiago Santa Catarina – Engenho [or Engenho] Engenho, Santa Catarina 15.083333, -23.666667 3036 382 3/84
Santiago Ribeira de Mato Sancho [or Ribeira de Mato Sanches] Mato Sanches stream [or Mato Sancho stream] 15.098698, -23.728967 1677 349 1/11
Santiago Picos Picos 15.081013, -23.636095 559 444 1/4
Santiago Ribeira dos Picos Picos stream 15.081738, -23.632877 1257 - 1/2
Santiago Praia Praia 14.942039, -23.516667 4271 69 2/27
Santiago Praia Formosa Praia Formosa 15.039177, -23.51306 1318 110 1/2
Santiago S. João Batista S. João Batista 14.95, -23.666667 3036 135 1/2
Santiago Santa Catarina [or Santa Catarina - Ilha do Sanque] Santa Catarina 15.110938, -23.716136 7374 293 2/81
Santiago Sedeguma Sedeguma, Santa Catarina 15.111, -23.685382 768 510 1/37
Santiago São Jorge dos Orgãos São Jorge dos Orgãos 15.052822, -23.601954 802 313 1/67
Santiago Tarrafal Tarrafal 15.275278, -23.749454 1634 21 2/3
Santiago Ilha de Santiago Santiago Island 15.103222, -23.622722 29199 242 3/40
Santo Antão Chão do Mocho Chão do Mocho 17.161455, -25.119461 2046 187 1/7
Santo Antão Ponta do Sol [or Ponta do Sol, Fantanha] Ponta do Sol [or Ponta do Sol, Fantanha] 17.200315, -25.091084 635 30 3/8
Santo Antão Porto Novo Porto Novo 17.025443, -25.066603 1493 47 3/120
Santo Antão Vale do Paúl [or Vale Paúl, Ribeira] Vale do Paúl [or Vale Paúl, stream] 17.148052, -25.013314 1217 7 1/12
Santo Antão Ilha de Santo Antão Santo Antão Island 17.064672, -25.170897 23523 1077 2/36
São Nicolau Arredores Vila Ribeira Brava Ribeira Brava surroundings 16.624659, -24.286525 1125 36 2/12
São Nicolau Cabeçalinho Cabeçalinho 16.602177, -24.306767 430 300 1/14
São Nicolau Calejão Calejão 16.604442, -24.301217 303 221 2/46
São Nicolau Carvoeiro Carvoeiro 16.648998, -24.303291 428 99 2/50
São Nicolau Chanzinha Chanzinha 16.616616, -24.29656 507 107 1/5
São Nicolau entre Jucalinho e Carrissal between Jucalinho and Carrissal 16.591956, -24.088655 6465 467 2/18
São Nicolau entre Ribeira Brava e Juncalinho between Ribeira Brava and Juncalinho 16.643644, -24.216589 12531 54 1/8
São Nicolau Perguiça Perguiça 16.563247, -24.281361 301 61 2/19
São Nicolau Campo de Aviação da Preguiça Preguiça Airfield 16.588013, -24.285383 1296 181 3/36
São Nicolau Porto da Preguiça Preguiça port 16.561723, -24.280177 159 6 1/19
São Nicolau Ribeira da Prainha Ribeira da Prainha 16.620503, -24.290903 1719 96 1/24
São Nicolau Ribeira das Queimadas Ribeira das Queimadas 16.641588, -24.315379 300 241 2/18
São Nicolau Ribeira de Caixa Ribeira de Caixa [undetermined locality] – – – 1/4
São Nicolau Ribeira João Ribeira João [undetermined locality] – – – 2/29
São Nicolau Ribeira Maiamba Ribeira Maiamba [undetermined locality] – – – 3/21
São Nicolau Ribeira Seca Ribeira Seca 16.608037, -24.212366 24792 175 1/15
São Nicolau S. João S. João 16.602678, -24.215949 22806 200 1/6
São Nicolau Pousada S. Nicolau S. Nicolau hostel [undetermined locality] – – – 1/1
São Nicolau Taboleiro Taboleiro [undetermined locality] – – – 3/44
São Nicolau Vila da Ribeira Brava [or Vale Paúl, Ribeira] Ribeira Brava [or Vale Paúl, Ribeira] 16.616667, -24.3 301 142 2/19
São Nicolau Ilha de São Nicolau São Nicolau Island 16.608037, -24.212366 24792 175 3/66
São Vicente Baia das Gatas Baia das Gatas 16.903244, -24.909886 301 6 1/18
São Vicente Calhau Calhau 16.853122, -24.86641 1482 8 1/13
São Vicente Mato Inglês Mato Inglês 16.860123, -24.946237 649 191 1/39
São Vicente São Pedro São Pedro 16.828744, -25.060674 1821 6 1/29
São Vicente Ribeira Passarão Ribeira Passarão 16.869242, -24.979704 1425 27 1/15
São Vicente Monte Sossego Monte Sossego 16.86152, -24.999323 414 30 1/17
Fogo Posto de Fogo, Cavaleiros Vale de Cavaleiros lighthouse 14.923969, -24.501743 300 44 1/1
Fogo S. Filipe São Filipe 14.899416, -24.494312 1484 132 4/27
Fogo Viveiros de S. Filipe S. Filipe vivarium 14.893628, -24.494822 1559 92 2/5
Fogo Ilha do Fogo Fogo Island 14.912456, -24.374662 16064 2004 3/6
Branco Ilhéu Branco Branco Islet 16.658552, -24.670287 2405 276 1/5
Raso Ilhéu Raso Raso Islet 16.617111, -24.586456 2405 48 3/156
Rombos Ilhéus Rombos Rombos Islets 14.96853, -24.644065 2484 9 1/7
10.3897/zookeys.1052.64607.figure9 5168200 901803C1-BB68-5249-A4D8-45C099EB58A8

Distribution of the Cabo Verde localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572777

Part of the material assigned to the genus Chioninia was studied and published by Margarida Pinheiro on two different occasions (Pinheiro 1986, 1990), while a few geckos of the genera Hemidactylus and Tarentola were used by José Jesus for sequencing and phylogenetic analysis (Jesus et al. 2001, 2002). More recently the collection has been consulted by Raquel Vasconcelos (1980–to date) for several ecological and dietary studies of species of the genus Tarentola. No type material is present in the collection.

This is the largest known herpetological collection from Cabo Verde, with 1740 specimens, and is followed by relevant collections from the MNHN with a total of 137 specimens, the NHMUK with 104 specimens, the MCNB with 102 specimens, and the TCWC with 97 specimens (data retrieved from GBIF.org in March 2020). Other collections worldwide generally do not exceed 50 specimens (data retrieved from GBIF.org in February 2021).

The dataset of this collection is available at GBIF (Ceríaco et al. 2018b; https://www.gbif.org/dataset/e5dd4343-8bd9-449a-bef5-47c655968225).

 Guinea-Bissau collection

The herpetofauna of Guinea-Bissau is one of the most poorly known in the region. The most recent checklist was published by Auliya et al. (2012), listing respectively 25 and 73 species of amphibians and reptiles. Historically the country has been poorly surveyed in terms of its biodiversity, with the first available data on the country’s herpetofauna provided by Bocage (1866, 1867, 1872, 1873, 1896a, b), Ferreira (1902), and Boulenger (1905, 1906). The Swiss naturalist Albert Monard provided the first country-wide revision of the herpetofauna (Monard 1940a, b), which included the description of new species (Agama boensis, Latastia ornata) and the first records of previously unrecorded species for the country. This collection is still extant in the MHNC and is composed of a total of 329 (191 amphibians and 138 reptiles) specimens. From 1944 to 1946, Fernando Frade led an extensive zoological expedition to the country (Frade et al. 1946), which resulted in the collection of approximately a thousand specimens that were later studied by Sara Manaças and constitute the bulk of the IICT collections (see below). Besides the Auliya et al. (2012) report on the herpetofauna of Bijagós, and two small contributions on amphibians (Rebelo 2018a) and reptiles (Rebelo 2018b) from “João Vieira e Poilão” National Park and a master’s thesis on the amphibians and reptiles of Boé area (Cabuy 2014), no modern data exists on its terrestrial herpetofauna.

The IICT collection comprises 980 specimens (604 amphibians and 376 reptiles), covering 12 species of amphibians and 37 species of reptiles (Table 3), corresponding to roughly half of the known taxa for the country. The amphibian collection comprises representatives of seven families and eight genera. These specimens are dominated by representatives of common species of the following families: Dicroglossidae, with 252 representatives of Crowned Bullfrogs, Hoplobatrachus occipitalis (Günther, 1858); Bufonidae, with 188 representatives of African Common Toads, Sclerophrys regularis; and Ptychadenidae, with 99 representatives of Mascarene Grass Frogs, Ptychadena mascareniensis (Duméril & Bibron, 1841), 27 representatives of Bibron’s Grass Frog, Ptychadena bibroni (Hallowell, 1845), and a single representative of Ansorge’s Grass Frog, Ptychadena cf. ansorgii (Boulenger, 1905). The first four taxa make up about 94% of the amphibian specimens, and approximately 60% of the entire collection. The reptile collection, despite being smaller, is taxonomically more diverse. It comprises representatives of 15 families and 26 genera. Within Squamates, the families Scincidae, Agamidae, and Lamprophiidae are the best represented, with 82, 82, and 52 specimens respectively. Families Lamprophiidae and Colubridae are those which have a greater diversity of species (seven species each), while the remaining families have no more than three species represented in the collection.

Overview of the Guinea Bissau amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
ANURA Duméril, 1806
Pipidae Gray, 1825 Pseudhymenochirus Chabanaud, 1920 Pseudhymenochirus merlini Chabanaud, 1920 Pitche – IICT/A 221-1946 Manaças (1947) 1
Xenopus Wagler, 1827 Xenopus tropicalis (Gray, 1864) Bissau – IICT/A 106 to 115–1945; Calequisse, Chachungo – IICT/A 143-1945 Manaças (1947) 11
Bufonidae (Gray, 1825) Sclerophrys Tschudi, 1838 Sclerophrys regularis (Reuss, 1833) Bissalanca – IICT/A 15–20/1945; Bissau – IICT/A GB1; Bissorã – IICT/A 171–274/1945; Cacine – IICT/A 72–89/1946, 91–93/1946; Mansoa [or Mansôa] – IICT/A 17/1946; Marques Mano – IICT/A 1–10/1945, 166/1945; Pecixe – IICT/A 145–149/1945, 151–159/1945; Pitche – IICT/A 164–257/1946; Tor – IICT/A 28–31/1945; Manaças (1949) 179
Sclerophrys sp. Bissorã – IICT/A 258–266/1945 9
Hyperoliidae Laurent, 1943 Hyperolius Rapp, 1842 Hyperolius concolor (Hallowell, 1844) Bissalanca – IICT/A 167/1945; Mansoa [or Mansôa] – IICT/A 25-1946; Marques Mano Farm – IICT/A 160–163/1945, 165/1945, 168–170/1945; Pitche – IICT/A 178-1946 Manaças (1949) 11
Hyperolius sp. between Buba and Corubal river – IICT/A-1990; Caiomete – IICT/A 131–132/1945; Catió – IICT/A GB2; Marques Mano Farm – IICT/A 1–4/1947, 164/1945 Manaças (1949) 9
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena ansorgii (Boulenger, 1905) Cacine – IICT/A 342-1946 1
Ptychadena bibroni (Hallowell, 1845) Bissorã – IICT/A 50–52/1946; Cutiá, Mansoa [or Mansôa] – IICT/A 26–49/1946 Manaças (1949) 27
Ptychadena mascareniensis (Duméril & Bibron, 1841) Bissorã – IICT/A 194–195/1945, 284–333/1946, 289–295/1945; Cacine – IICT/A 66–71/1946, 75/1946; Catió – IICT/A 66–71/1946, 102–103/1946, 106–112/1946, 141/1946; Catió, Cufar – IICT/A 151/1946; Cutiá, Mansoa [or Mansôa] – IICT/A 283/1946; Gabu] – IICT/A 97/1945; Madina do Boé – IICT/A 230–243/1945; Mansoa [or Mansôa] – IICT/AIICT/A 8–1946; Prabis – IICT/A 50–54/1945 Manaças (1949) 99
Dicroglossidae Anderson, 1871 Hoplobatrachus Peters, 1863 Hoplobatrachus occipitalis (Günther, 1858) Bafatá – IICT/A 100–105/1945; Bissalanca – IICT/A 12–14/1945; Bissau – IICT/A 116–119/1945; Bissorã – IICT/A 191–193/1945, 196–207/1945, 246–250/1945, 254–257/1945, 268–273/1945, 275–288/1945; Calequisse, Chachungo – IICT/A 154–156/1945; Cachungo – IICT/A 120–130/1945, 133–142/1945; Catió – IICT/A 94–98/1946, 104/1946, 116/1946, 120–140/1946, 142–147/1946; 156–169/1946; Catió, Cufar – IICT/A 148–150/1946, 152–155/1946; Farim – IICT/A 68–96/1945; Gabu – IICT/A 98–99/1945; Mansoa [or Mansôa] – IICT/A 1–7/1946, 9–24/1946, 53–65/1946, 258–282/1946, 336–341/1946; Parabis – IICT/A 36–49/1945; Pecixe – IICT/A 150/1945; Marques Mano Farm – IICT/A 11/1945; São Domingos – IICT/A 55–57/1945; Tor – IICT/A 21–27/1945, 32–35/1945 Manaças (1949) 252
Ranidae Batsch, 1796 Amnirana Dubois, 199 Amnirana galamensis (Duméril & Bibron, 1841) Catió – IICT/A 99–101/1946 Manaças (1949) 3
GYMNOPHIONA Müller, 1832
Dermophiidae Taylor, 1969 Geotrypetes Peters, 1880 Geotrypetes seraphini (Duméril, 1859) Machado Farm – IICT/A 296–297/1945 2
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 604
REPTILES
CROCODYLIA Gmelin, 1789
Crocodylidae Cuvier, 1808 Crocodylus Laurenti, 1768 Crocodylus suchus Geoffroy, 1807 Bijimita – IICT/R 102–109/1954 8
SQUAMATA Oppel, 1811
Gekkonidae Gray, 1825 Hemidactylus Goldfuss, 1820 Hemidactylus angulatus Hallowell, 1854 Bissalanca – IICT/R 97/1945, 145/1945, 184/1945; Bissau – IICT/R 152/1945, 128–130/1946; Buba – IICT/R GB4; Cacine – IICT/R 17/1946, 19/1946/, 28/1946; Chitole [or Xitole] – IICT/R 12/1946; Mansoa [= Mansôa] – IICT/R 1/1946; Marques Mano Farm – IICT/R 2–3/1944, 44/1945, 60–61/1945, 257–259/1945, 262/1945, 264/1945, 267/1945, 269/1945, 278/1945; Gabu – IICT/R A–B/1962; São Vicente – IICT/R 1A-1946 Manaças (1951) 29
Phyllodactylidae Gamble, Bauer, Greenbaum & Jackman Tarentola Gray, 1825 Tarentola ephippiata senegambiae Joger, 1984 Bafatá – IICT/R 291/1945; Pitche – IICT/R 122/1946 Manaças (1951) 2
Amphisbaenidae Gray, 1825 Cynisca Duméril & Bibron, 1839 Cynisca feae (Boulenger, 1906) Bissalanca – IICT/R 64/1945; Bissau – IICT/R 53/1945; Marques Mano Farm – IICT/R 28–30/1945, 40/1945, 42–43/1945, 46–48/1945, 54/1945, 65–71/1945, 76–77/1945, 76A/1945, 92–96/1945, 138–139/1945 Manaças (1955) 29
Varanidae Hardwicke & Gray, 1824 Varanus Merrem, 1820 Varanus exanthematicus (Bosc, 1792) Bissalanca – IICT/R 45/1945, 51/1945, 80/1945; Brene – IICT/R 52/1945; Cachungo – IICT/R 192/1945, 201/1945; Pecixe – IICT/R 219–220/1945; Pitche – IICT/R 112/1945; Tor – IICT/R 118/1945 Manaças (1955) 10
Varanus niloticus (Linnaeus, 1758) Ilha Formosa – IICT/R 155/1945 1
Scincidae Cuvier, 1808 Mochlus Günther, 1864 Mochlus guineensis Peters, 1879 Cacine – IICT/R 29/1946 Manaças (1951) 1
Trachylepis Fitzinger, 1843 Trachylepis affinis (Gray, 1838) Bissalanca – IICT/R 183/1945; Bissorã – IICT/R 305–306/1945; Cacine – IICT/R 15–16/1946, 18/1946, 25–27/1946, 30/1946; Calequisse, Chachungo – IICT/R 202/1945; Madina do Boé – IICT/R 77–94/1946; Marques Mano Farm – IICT/R 4–5/1945, 9/1945, 137/1945, CZ000011298–11300; Pecixe – IICT/R 215–216/1945; Pitche – IICT/R 50/1946, 95–111/1946 Manaças (1951) 56
Trachylepis perroteti (Duméril & Bibron, 1839) Bissorã – IICT/R 309/1945; Cacine – IICT/R 31/1946; Calequisse, Chachungo – IICT/R 193–199/1945, 203/1945; Marques Mano Farm – IICT/R 32–33/1945, 254–255/1945, 254A–255A/1945, 256/1945, 260/1945, 261/1945, 261A/1945, 265/1945, 265A/1945, 266/1945, Guiné-Bissau [undetermined locality] – IICT/R 46/1964, 46ª/1964 Manaças (1951) 25
Agamidae Gray, 1827 Agama Daudin, 1802 Agama cf. boensis Monard, 1940 Pitche – IICT/R 44/1946, 76/1946; Guiné-Bissau [undetermined locality] – IICT/R GB6 Manaças (1951) 3
Agama picticauda Peters, 1877 Bafatá – IICT/R 290/1945; Bambadinca – IICT/R 175/1945; Bissau, city center – IICT/R 228/1945, Bissorã – IICT/R 298/1945, 310–311/1945; Cachungo – IICT/R 205–209/1945, 209A/1945, 211–214/1945; Chitole [or Xitole] – IICT/R 8–11/1946; Cutiá, Mansoa [or Mansôa] – IICT/R 4/1946; Gabu – IICT/R 159–166/1945; Ilha Formosa – IICT/R 157–158/1945; Mansoa [or Mansôa] – IICT/R 5/1946; Marques Mano Farm – IICT/R 11–13/1945, 34–36/1945, 98/1945; Pecixe – IICT/R 185–187/1945, 189/1945, 218/1945, 221–223/1945; Pitche – IICT/R 47–49/1946, 51–65/1946, 67–75/1946, 121/1946; Tor IICT/R 119–121/1945, 140–141/1945 Manaças (1951) 79
Agama sp. Guiné-Bissau [undetermined locality] – IICT/R GB7 1
Chamaeleonidae Gray, 1825 Chamaeleo Laurenti, 1768 Chamaeleo gracilis Hallowell, 1842 Bissorã – IICT/R 209/1945, 301–302/1945, 304/1945, 308/1945; Brene – IICT/R 62/1946; Cacine – IICT/R 14/1946, 20/1946, 32/1946; Catió – IICT/R 39–40/1946; Chitole [or Xitole] – IICT/R 13/1946; Mansoa [or Mansôa] – IICT/R 2/1946; Nhampurbani, Pitche – IICT/R 123/1946; Marques Mano Farm – IICT/R 31/1945, 72/1945; Parabis – IICT/R 146–151/1945; Tor – IICT/R 123–129/1945, 132–133/1945 Manaças (1951) 31
Chamaeleonidae Gray, 1825 Chamaeleo Laurenti, 1768 Chamaeleo senegalensis Daudin, 1802 Bijimita – IICT/R 130/1945; Bissorã – IICT/R 294–295/1945, 297/1945, 299–300/1945; 307/1945; Mansoa [or Mansôa] – IICT/R 3/1946 8
SERPENTES
Typhlopidae Merrem, 1820 Afrotyphlops Broadley & Wallach, 2009 Afrotyphlops punctatus (Leach, 1819) Marques Mano Farm – IICT/R 1/1939, 5/1947; Guiné-Bissau [undetermined locality] – IICT/R 282/1945 Manaças (1955) 3
Leptotyphlopidae Stejneger, 1892 Myriopholi Hedges, Adalsteinsson & Branch, 2009 Myriopholis narirostris (Peters, 1867) Marques Mano Farm – IICT/R 49/1945 Manaças (1955) 1
Pythonidae Fitzinger, 1826 Python Daudin, 1803 Python sebae (Gmelin, 1789) Mandinga – IICT/R GB10 1
Viperidae Oppel, 1811 Bitis Gray, 1842 Bitis arietans (Merrem, 1820) Marques Mano Farm – IICT/R 283/1945 1
Causus Wagler, 1830 Causus maculatus (Hallowell, 1842) Cachungo – IICT/R 240/1945 1
Lamprophiidae Fitzinger, 1843 Atractaspis Smith, 1849 Atractaspis aterrima Günther, 1863 Buba-Tombó – IICT/R GB11; Cacine – IICT/R 35/1946; Machado Farm – IICT/R 11/1947; Guiné-Bissau [undetermined locality] – IICT/R GB12 4
Boaedon Duméril, Bibron & Duméril, 1854 Boaedon fuliginosus (Boie, 1827) Bijimita – IICT/R 102/1945; Bissalanca – IICT/R 79/1945; Bissau – IICT/R 177/1945; Brene – IICT/R 74/1945; Marques Mano Farm – IICT/R 1/1944, 26/1945, 50/1945, 74/1945; Machado Farm – IICT/R 12/1987 Manaças (1955) 8
Boaedon sp. Marques Mano Farm – IICT/R 1/1944; Tor – IICT/R 113/1945 Manaças (1955) 2
Limaformosa Broadley, Tolley, Conradie, Wishart, Trape, Burger, Kusamba, Zassi-Boulou & Greenbaum, 2018 Limaformosa crossi (Boulenger, 1895) Tor – IICT/R 112/1945, 243/1945 2
Lycophidion Fitzinger, 1843 Lycophidion albomaculatum Steindachner, 1870 Bissalanca – IICT/R 21/1945, 241/1945, Bissau – IICT/R 229/1945; Marques Mano Farm – IICT/R 27/1945; Guiné-Bissau [undetermined locality] – IICT/R GB14 Manaças (1955) 5
Lycophidion irroratum (Leach, 1819) Marques Mano Farm – IICT/R 135/1945; Machado Farm – IICT/R 275–276/1945, 280/1945 Manaças (1955) 4
Psammophis Boie, 1825 Psammophis elegans (Shaw, 1802) Bissalanca – IICT/R 22–23/1945, 58/1945; Catió – IICT/R 38/1946; Marques Mano Farm – IICT/R 14 15/1945; Pecixe – IICT/R 239/1945; Tor – IICT/R 88/1945, 111/1945, 117/1945 Manaças (1955) 10
Psammophis lineatus (Duméril, Bibron & Duméril, 1854) Bafatá – IICT/R 2/1953; Catió – IICT/R 3871946; Machado Farm – IICT/R 263/1945 Manaças (1955) 3
Psammophis afroccidentalis Trape, Böhme & Mediannikov, 2019 Bijmita – IICT/R 101/1945; Bissau – IICT/R 126/1946; Cacine – IICT/R 21–22/1946, 34/1946; Catió – IICT/R 37/1946; Mandinga – IICT/R B9; Marques Mano Farm – IICT/R 25/1945; Tor – IICT/R 122/1945, 134/1945, 134A/1945, 142–143/1945 Manaças (1955) 13
Psammophis sp. Guiné-Bissau [undetermined locality] – IICT/R GB1 1
Elapidae Boie, 1827 Naja Laurenti, 1768 Naja haje (Linnaeus, 1758) Guiné-Bissau [undetermined locality] – IICT/R 227 1
Colubridae Oppel, 1811 Crotaphopeltis Fitzinger, 1843 Crotaphoepltis hotamboeia (Laurenti, 1768) Bafatá – IICT/R 287/1945; Bijimita – IICT/R78/1945; Bissalanca – IICT/R 103/1945, 24871945, 124/1946; Bissau, city center – IICT/R 10/1945, 182/1945; Machado Farm – IICT/R 277/1945; Guiné-Bissau [undetermined locality] – IICT/R GB13 Manaças (1955) 9
Dasypeltis Wagler, 1830 Dasypeltis confusa Trape & Mané, 2008 Bissalanca – IICT/R 231/1945 Manaças (1955) 1
Colubridae Oppel, 1811 Dasypeltis Wagler, 1830 Dasypeltis gansi Trape & Mané, 2006 Cachamba – IICT/R GB5 1
Dispholidus Duvernoy, 1832 Dispholidus typus (Smith, 1828) Cacine – IICT/R 23/1946 Manaças (1955) 1
Grayia Günther, 1858 Grayia smithi (Leach, 1818) Machado Farm – IICT/R 15/1947 Manaças (1955) 1
Philothamnus Smith, 1840 Philothamnus irregularis (Leach, 1819) Bijimita – IICT/R 100/1945, 104/1945; 115/1945; Bissalanca – IICT/R 181/1945, 20/1945; Bissau – IICT/R 230/1945; Bissorã – IICT/R 303/1945; Bolama – IICT/R GB3; Marques Mano – IICT/R 136/1945; Tor– IICT/R 89–91/1945, 114/1945; Guiné-Bissau [undetermined locality] – IICT/R 6/1944, 10/1944, GB8 16
Toxicodryas Hallowell, 1857 Toxicodryas blandingii (Hallowell, 1844) Ilha de Bubaque – IICT/R GB2; Bissalanca – IICT/R 242/1945, 244/1945; Marques Mano Farm – IICT/R 16/1945 Manaças (1955) 4
TOTAL NUMBER OF REPTILES SPECIMENS 376

Geographically, these specimens come from 38 different localities distributed in the nine regions of the country – Bafatá, Biombo, Bissau, Bolama, Cacheu, Gabu, Oio, Quinara, and Tombali (Table 4; Fig. 10). The temporal range of collecting events ranges from 1939 to 1997, although the bulk of the specimens were collected between 1944 and 1946. The main contributors to the collections were Fernando Frade and his team during the Zoological Missions to Guinea Bissau (1944–1946), although different individuals collected other specimens on other occasions and dates. This material was primarily studied and published by Sara Manaças on five different occasions (Manaças 1947, 1949, 1950a, 1951a, b, 1955). Gans (1987) used specimens of Cynisca feae (Boulenger, 1906) (IICT 28/1945; 29/1945; 40/1945; 42/1945; 46/1945; 47/1945; 53/1945; 64/1945; 65/1945; 66/1945; 70/1945; 71/1945; 76/1945; 77/1945; 93/1945; 94/1945; 138/1945) in a taxonomic revision of the genus Cynisca (Squamata: Amphisbaenidae). Some of the snake specimens were used by Manaças (1982) to produce a checklist and identification keys for the venomous snakes of the Portuguese speaking countries in Africa. No further material was ever cited in subsequent papers and the collection has remained almost inaccessible until now. In the course of our rehousing effort, previously unpublished specimens were located, including the first confirmed record of the Gaboon Caecilian, Geotrypetes seraphini (Duméril, 1859) for the country (Marques and Ceríaco pers. obs.). Specimens from this collection are currently being used for ongoing taxonomic revisions and graduate students’ projects. The collection holds no type material.

Gazetteer of Guinea-Bissau localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Province Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Bafatá Bafatá Bafatá 12.166667, -14.666667 3036 8 5/10
Bafatá Bambadinca Bambadinca 12.152227, -14.476879 3036 49 1/1
Bijagós llha de Bubaque Bubaque Island 11.252357, -15.861174 6538 24 1/1
Bijagós Bolama Bolama 11.57671, -15.48019 3036 16 1/1
Bijagós Ilha Formosa Formosa Island 11.483333, -15.966667 12350 31 2/3
Biombo Bijimita Bijimita 11.883333, -15.85 3036 13 6/13
Biombo Ponta de Machado Machado Farm 11.84958, -15.642988 10000 42 9/15
Biombo Marques Mano Marques Mano Farm 11.84958, -15.642988 10000 42 20/104
Biombo Prabis Prabis [or Prábis] 11.8025, -15.738888 1008 22 3/25
Biombo Tor Tor 11.847634, -15.901396 3036 18 10/45
Bissau Bissalanca Bissalanca 11.883333, -15.666667 3036 37 14/32
Bissau Bissau Bissau 11.857056, -15.58711 7327 15 7/19
Bissau Bissau, Cidade Bissau, city center 11.858736, -15.579213 301 14 4/8
Bissau Brene Brene 11.883333, -15.65 301 35 3/3
Cacheu Caiomete Caiomete 11.985172, -16.234847 1000 8 1/2
Cacheu Calequisse, Canchingo Calequisse, Chachungo 12.069722, -16.224444 900 25 4/13
Cacheu Chachungo Chachungo 12.074424, -16.02772 825 31 4/34
Cacheu Pecixe Pecixe 11.810861, -16.089651 3036 8 6/25
Cacheu São Domingos São Domingos 12.401944, -16.200556 3036 10 1/3
Cacheu São Vicente S. Vicente 12.230989, -15.755374 1566 14 1/1
Gabu Gabu [or Nova Lamego] Gabu 12.283333, -14.216667 2000 48 4/13
Gabu Madina Boé Madina do Boé 11.75, -14.216667 3036 98 2/32
Gabu Mandinga [or Miniou, Mandinga] Mandinga 12.618496, -15.102543 6437 48 2/2
Gabu Nhampurbani, Pitche Nhampurbani, Pitche 12.333333, -13.95 3036 71 1/1
Gabu Pitche Pitche 12.3226, -13.95412 1352 66 8/121
Oio Bissoram Bissorã 12.223056, -15.4475 7500 15 11/189
Oio Cutiá, Mansoa Cutiá, Mansoa [or Mansôa] 12.174722, -15.240833 3036 35 3/26
Oio Farim Farim 12.483889, -15.221667 800 11 1/29
Quinara Buba Buba 11.592204, -14.988713 1687 40 1/1
Quinara Chitole Chitole [or Xitole] 11.733333, -14.816667 877 28 3/6
Quinara Entre Buba e o rio Coruball between Buba and Corubal river 11.646348, -14.893414 5000 23 1/1
Quinara Tombô-Fulacunda Buba-Tombó 11.65, -15.01667 1094 39
Tombali Cachamba Cachamba 11.191942, -15.0839 5168 18 1/1
Tombali Cacine Cacine 11.123447, -15.01538 1640 11 11/47
Tombali Catió Catió 11.283333, -15.25 301 9 8/56
Tombali Catió - Cufer Catió, Cufer 11.291869, -15.175909 700 9 2/8
Guiné-Bissau Guiné-Bissau, undetermined locality 10/13
10.3897/zookeys.1052.64607.figure10 5168170 6A6EAF01-BC97-596A-B3CF-DB35CF46ED9C

Distribution of the Guniea-Bissau localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572778

Specimens from Guinea-Bissau are scarce in collections worldwide. The main collections holding specimens of amphibians and reptiles from Guinea-Bissau are the IICT, with 980 specimens; ZFMK, with 108 specimens (Aulyia et al. 2012); the MHNC, with 329 specimens (Monard 1940a, b), and the MNHN with 46 specimens (data retrieved from GBIF.org in February 2021). Other specimens exist in several other museums but generally not exceeding 10 specimens per collection (data retrieved from GBIF.org in March 2020).

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018a; https://www.gbif.org/dataset/c6e94ce4-5d25-4758-8a52-2d5ee4d520fc).

 São Tomé & Príncipe collections

Since the early 2000's the herpetofauna of São Tomé & Príncipe has been subject of several studies, including the descriptions of various new species of amphibians and reptiles. A provisional checklist of the terrestrial herpetofauna of São Tomé, Príncipe, and Annobon was provided by Ceríaco et al. (2018a). This work listed eight species of amphibians (five for São Tomé and three for Príncipe) and 23 species of reptiles (12 for São Tomé and 13 for Príncipe). New studies have since contributed to the expansion of our knowledge of the herpetofauna of the country, including the description of a new species of the genus Boaedon (Ceríaco et al. 2021), and several revisions have resurrected synonymised taxa, as the case of Schistometopum ephele (O’Connell et al. in press). Exemplifying this constant flux of work, the recent publication of two major reviews on the herpetofauna of these islands by Bell et al. (in press) and Ceríaco et al. (2021) provide detailed historical overviews on the study of the amphibians and reptiles and updated the known number of species to eight amphibians and 22 reptiles.

The collection comprises 234 specimens (76 amphibians and 158 reptiles), eight species of amphibians and 20 species of reptiles (Table 5), corresponding to 80% of the known taxa for the country. The amphibian collection comprises representatives of five families and five genera corresponding to six anurans and one caecilian. The family Dermophiidae is the best represented with 43 individuals of the São Tomé Caecilian, Schistometopum thomense (Bocage, 1873), endemic to São Tomé Island. The family Phrynobatrachidae is the second best represented within the collection with a total of 15 individuals, of which nine correspond to the Príncipe Puddle Frog, Phrynobatrachus dispar (Peters, 1870), endemic to Príncipe Island, and six correspond to the Calm Puddle Frog, Phrynobatrachus leveleve Uyeda, Drewes & Zimkus, 2007, endemic to São Tomé Island. All known amphibian species are present in the collection with the exception of the Moller’s Reed Frog, Hyperolius molleri (Bedriaga 1892). The reptile collection comprises representatives of six families and 11 genera, all squamates. The families Scincidae, Lamprophiidae, and Colubridae are the best represented in the collection with representatives of all known taxa for the country with 76, 23, and 21 specimens respectively. The Scincidae is the family with the greatest diversity of species.

Overview of the São Tomé & Príncipe’s amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
ANURA Duméril, 1806
Hyperoliidae Laurent, 1943 Hyperolius Rapp, 1842 Hyperolius drewesi Bell, 2016 Roça Esperança – IICT/A 13A/1954 1
Hyperolius thomensis Bocage, 1886 Roça Saudade – IICT/A STP6; São Tomé Island [undetermined locality] – IICT/A STP5 2
Arthroleptidae Mivart, 1869 Arthroleptis Smith, 1849 Leptopelis palmatus (Peters, 1868) Príncipe Airport – IICT/A 52–53/1955, 54–55/1956; Santo António – IICT/A 13–14/1955; Ribeira Izé – IICT/A 44–45/1955 Manaças (1958) 8
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena newtoni (Bocage, 1886) Roça Monte Café – IICT/A 1/1961; São Tomé Island [undetermined locality] – IICT/A 15/1967, 16A–16B/1967 4
Phrynobatrachidae Laurent, 1941 Phrynobatrachus Günther, 1862 Phrynobatrachus dispar (Peters, 1870) Príncipe Airport – IICT/A 46–51/1955; Roça Azeitona [or Roça São Jorge] – IICT/A STP7; Roça Esperança – IICT/A 15/1954, 18/1954 Manaças (1958) 9
Phrynobatrachus leveleve Uyeda, Drewes & Zimkus, 2007 Roça Monte Café – IICT/A 37/1954; São Tomé Island [undetermined locality] – IICT/A 2–4/1954, STP4A, 4B 6
GYMNOPHIONA Müller, 1832
Dermophiidae Taylor, 1969 Schistometopum Parker, 1941 Schistometopum thomense (Bocage, 1873) Potó-Correia – IICT/A 9/1966; Roça Milagrosa – IICT/A 1/1954, 9–12/1954, 9/1966, STP3B, 3C; Roça Monte Café – IICT/A 26–41/1954; Roça Pinheira – IICT/A 1/1967; Roça Ponta-Figo – IICT/A 56–57/1958, STP3A; Roça S. Nicolau – IICT/A 5/1954; Roça Saudade – IICT/A 1–9/1963, 20–25/1954 Manaças (1958) 43
Schistometopum ephele Taylor, 1965 Roça Porto Alegre – IICT/A 6–8/1954; Manaças (1958) 3
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 76
REPTILES
SQUAMATA Oppel, 1811
Gekkonidae Gray, 1825 Hemidactylus Goldfuss, 1820 Hemidactylus greeffii Bocage, 1886 Roça Nova Moca – IICT/R 12/1954, 20/1954 Manaças (1958) 2
Hemidactylus longicephalus Bocage, 1873 Santo António – 67–68/1955, 104–106/1955 Manaças (1958) 5
Hemidactylus mabouia (Moreau de Jonnès, 1818) Roça Esperança – IICT/R 40/1954, 43/1954; Roça Nova Moca – IICT/R 17–19/1954, 49/1954; Roça Potó-Correia – IICT/R 16–18/1966 Manaças (1958) 8
Hemidactylus principensis Miller, Sellas & Drewes, 2012 Príncipe Airport – IICT/R 102/1955 Manaças (1958) 1
Hemidactylus sp. Tinhosa Grande Islet – IICT/R 1/1970 1
Lygodactylus Gray, 1864 Lygodactylus delicatus Pasteur, 1962 Príncipe Airport – IICT/R 64–66/1955 Manaças (1958) 3
Scincidae Cuvier, 1808 Feylinia Gray, 1845 Feylinia polylepis Bocage, 1887 Roça Porto Real – IICT/R 28/1954, STP9; Roça Esperança – IICT/R 30–33/1954, 42/1954, STP9 Manaças (1958) 8
Panaspis Cope, 1868 Panaspis africana (Gray, 1845) Príncipe Airport – IICT/R 71/1955, 76/1955, 81–98/1955 Manaças (1958) 20
Panaspis thomensis Ceríaco, Soares, Marques, Bastos-Silveira, Scheinberg, Harris, Brehm & Jesus, 2018 Roça Monte Café – IICT/R 45/1954, 47–48/1954; Roça Nova Moca – IICT/R 2/1954, 50/1954 Manaças (1958); Soares et al. (2018) 5
Trachylepis Fitzinger, 1843 Trachylepis adamastor Ceríaco, 2015 Tinhosa Grande Islet – IICT/R 1–2/1970, 1–6/1971; Príncipe Airport – IICT/R 69/1955, 73–74/1955; Roça Esperança – IICT/R 35/1954 Ceríaco (2015); Ceríaco et al. (2016) 13
Scincidae Cuvier, 1808 Trachylepis Fitzinger, 1843 Trachylepis affinis (Gray, 1838) Príncipe Airport – IICT/R 70/1955, 72/1955, 77–80/1955, 99/1955; Roça Esperança – IICT/R 36–39/1954, 44/1954 Manaças (1958); Ceríaco et al. (2016) 14
Trachylepis thomensis Ceríaco, Marques & Bauer, 2016 Rolas Islet – IICT/R 3–7/1954; Roça Bela Vista – IICT/R 13–16/1954; Potó-Correia – IICT/R 11–16/1966; Roça Pinheira – IICT/R STP11; São Tomé Island [undetermined locality] – IICT/R 14/1967 Manaças (1958, 1973); Ceríaco et al. (2016) 17
SERPENTES
Typhlopidae Merrem, 1820 Afrotyphlops Broadley & Wallach, 2009 Afrotyphlops elegans (Peters, 1868) Príncipe Island [undetermined locality] – IICT/R 111–112/1956; Roça Esperança – IICT/R 29/1954; Santo António – IICT/R 27/1954 Manaças (1958) 4
Letheobia Cope, 1868 Letheobia newtoni (Bocage, 1890) Roça Monte Café – IICT/R 22–23/1954, STP3; Roça Potó-Correia – IICT/R 7/1966, 10/1966 Manaças (1958, 1973) 5
Letheobia sp. Roça Porto Real – IICT/R 6/1967, 11–12/1967 3
Lamprophiidae Fitzinger, 1843 Boaedon Duméril, Bibron & Duméril, 1854 Boaedon bedriagae Boulenger, 1907 Água-Izé – IICT/R 51/1954; Angra Toldo – IICT/R 2/1967; Ponta-Figo – IICT/R STP12; Potó-Correia – IICT/R 4/1966, 18/1967, 21/1967; Roça Boa Entrada – IICT/R 1771967; Roça Porto Alegre – IICT/R 8/1954; São Tomé Island [undetermined locality] – IICT/R 3/1966, 19/1967, STP13 Manaças (1973) 11
Boaedon mendesi Ceríaco, Arellano, Jadin, Marques, Parrinha & Hallermann, 2021 Príncipe Airport – IICT/R 63/1955; Roça Sundy – IICT/R 113–114/1956, 2671954, 8/1967; Santo António – IICT/R 109/1955; Príncipe Island [undetermined locality] – IICT/R 52–56/1955, 58–59/1955 Manaças (1958, 1973) 12
Elapidae Boie, 1827 Naja Laurenti, 1768 Naja peroescobari Ceríaco, Marques, Schmitz & Bauer, 2017 Ribeira Peixe – IICT/R 18/1972; Roça Porto Alegre – IICT/R 9/1954; Santa Josefina – IICT/R 20/1967; São Tomé Island [undetermined locality] – IICT/R 2/1966; Uba-Budo – IICT/R STP10 Manaças (1958, 1973); Ceríaco et al. (2017) 5
Colubridae Oppel, 1811 Hapsidophrys Fischer, 1856 Hapsidophrys principis (Boulenger, 1906) Príncipe Airport – IICT/R 103/1955, 107–108/1955; Roça São Jorge [or Roça Azeitona] – IICT/R 61–62/1955; Roça Sundy – IICT/R 41/1954; Príncipe Island [undetermined locality] – IICT/R 60–61/1955, 110/1956, STP2 Manaças (1958) 10
Philothamnus Smith, 1840 Philothamnus thomensis Bocage, 1882 Água-Izé – IICT/R 10/1954; Angra Toldo – IICT/R 3/1967; Ponta-Figo – IICT/R 115–117/1958; Roça Potó-Correia – IICT/R 5–8/1966, 13/1967; Roça Milagrosa – IICT/R 21/1954; São Tomé Island [undetermined locality] – IICT/R STP1 Manaças (1958, 1973) 11
TOTAL NUMBER OF REPTILES SPECIMENS 158

Geographically these specimens come from 23 different localities distributed in the two islands, São Tomé and Príncipe, and in two small islets on each coast of the main islands, respectively, Rolas and Tinhosa Grande (Table 6; Fig. 11). The temporal range of collecting events is from 1954 to 1971, although the bulk of the specimens were collected in the years 1954, 1956, and 1966. The main contributors to the collections were Fernando Frade and his team during the Scientific Mission to São Tomé and Príncipe (1954), and Décio Passos (birth and death dates unknown), an airport worker in Príncipe, who collected and offered a good series of specimens during 1955 and 1956. This material was primarily studied and published by Sara Manaças on two different occasions (Manaças 1958, 1973). In recent years the importance of this collection has increased with the descriptions of six new species, all based on the IICT material (Ceríaco 2015; Ceríaco et al. 2016, 2017, 2021a; Soares et al. 2018), which have resulted in a good series of types in the collection, including the Adamastor Skink, Trachylepis adamastor Ceríaco, 2015 (holotype and seven paratypes), the São Tomé Leaf-litter Skink, Panaspis thomensis Ceríaco, Soares, Marques, Bastos-Silveira, Scheinberg, Harris, Brehm & Jesus, 2018 (two paratypes), the São Tomé Cobra, Naja peroescobari Ceríaco, Marques, Schmitz & Bauer, 2017 (three paratypes), and the Príncipe Jita Snake, Boaedon mendesi Ceríaco, Arellano, Jadin, Marques, Parrinha & Hallermannn, 2021 (five paratypes). These specimens have also been used in phylogeographic studies (Ceríaco et al. 2020c).

Gazetteer of São Tomé & Príncipe localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Island/Islet Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Príncipe Aeroporto Príncipe Airport 1.662259, 7.41235 1039 180 9/50
Príncipe Roça Azeitona Roça Azeitona [or Roça São Jorge] 1.666667, 7.383333 301 171 2/3
Príncipe Ilha do Príncipe [or Príncipe] Príncipe Island 1.612248, 7.396894 11813 149 3/12
Príncipe Porto Real [or Roça Porto Real] Roça Porto Real 1.624118, 7.405149 656 132 2/5
Príncipe Roça Esperança Roça Esperança 1.635551, 7.398481 154 188 7/18
Príncipe Roça Sundy Roça Sundy 1.668775, 7.383353 852 169 2/5
Príncipe Santo António Santo António 1.636944, 7.419444 790 12 4/9
São Tomé Água-Izé [or Ribeira Izé] Água-Izé [or Ribeira Izé] 0.217868, 6.725149 210 38 3/4
São Tomé Angra Toldo Angra Toldo 0.15770, 6.67070 301 19 2/2
São Tomé Ponta-Figo [or Roça Ponta-Figo] Ponta-Figo [or Roça Ponta-Figo] 0.339465, 6.54286 920 151 3/7
São Tomé Potó-Correia [or Roça Potó-Correia] Potó-Correia [or Roça Potó-Correia] 0.29685, 6.680288 1000 291 6/18
São Tomé Ribeira Peixe Ribeira Peixe 0.09028, 6.61528 301 19 1/1
São Tomé Roça Bela Vista Roça Bela Vista 0.366667, 6.7 3036 27 1/4
São Tomé Roça Boa Nova Roça Boa Entrada 0.35, 6.666667 3036 180 1/1
São Tomé Roça Milagrosa Roça Milagrosa 0.27978, 6.65995 211 465 2/8
São Tomé Roça Monte-Café Roça Monte Café 0.299931, 6.64031 311 694 5/23
São Tomé Roça Nova Moca Roça Nova Moca 0.287436, 6.63414 339 854 3/8
São Tomé Pinheira [or Pinheiro] Roça Pinheira 0.286313, 6.716026 44 118 2/2
São Tomé Roça Porto Alegre Roça Porto Alegre 0.033333, 6.533333 634 16 3/5
São Tomé Roça S. Nicolau Roça S. Nicolau 0.279608, 6.625934 427 917 1/1
São Tomé Roça Saudade Roça Saudade 0.283333, 6.633333 3036 771 2/16
São Tomé Santa Josefina Santa Josefina 0.066667, 6.533333 3036 109 1/1
São Tomé Uba-Budo Uba-Budo 0.262, 6.702 333 254 1/1
São Tomé São Tomé São Tomé Island 0.232334, 6.598798 25289 695 7/15
Rolas Ilhéu das Rolas Rolas Islet -0.002066, 6.521748 1365 89 1/5
Tinhosa Grande Pedras Tinhosas Tinhosa Grande Islet 1.342118, 7.292135 117 55 2/9
10.3897/zookeys.1052.64607.figure11 5168172 E3B18897-DEFD-5A22-92EC-E7A9F8CF5D61

Distribution of the São Tomé (A) & Príncipe (B) localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572779

The main collections holding specimens of amphibians and reptiles from São Tomé & Príncipe are CAS with 1562 specimens, followed by UMMZ with 1106, and USNM with 778 specimens (data retrieved from GBIF.org in February 2021). The IICT collection, together with the collection hosted at MUHNAC, is the fourth largest collection with a total of 457 specimens.

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018b; https://www.gbif.org/dataset/c6e94ce4-5d25-4758-8a52-2d5ee4d520fc).

 Angola collection

After approximately four decades of violent armed conflict that closed the country to researchers, the study of Angolan herpetofauna has experienced a rapid growth in the last decade. The most recent checklists of the herpetofauna of Angola report 117 species of amphibians and 278 reptiles, of which 54 are endemic to the country (Marques et al. 2018; Baptista et al. 2019; Branch et al. 2019). However, the available checklists are rapidly becoming outdated due to the rapid growth of species discovery and new species descriptions (see Branch 2018; Marques et al. 2019a, b, 2020; Ceríaco et al. 2020a, b; Hallermannn et al. 2020). Marques et al. (2018) provide a comprehensive review of the history of herpetological research in Angola.

The collection comprises 677 specimens (259 amphibians and 418 reptiles), covering 21 species of amphibians and 45 species and 2 subspecies of reptiles (Table 7). A total of ten families of amphibians are represented in the collection comprising eleven different genera, all anurans. Ptychadenidae is the best represented family with 45 specimens of the multiple cryptic group of Mascarene Grass Frog, Ptychadena cf. mascareniensis; 16 specimens of Spotted Grass Frog, Ptychadena subpunctata (Bocage, 1866); 17 specimens of a series of tadpoles and juveniles of an unidentified Grass Frog Ptychadena sp.; two specimens of Grandison’s Grass Frog, Ptychadena grandisonae Laurent, 1954; and a single specimen of both Anchieta’s Grass Frog, Ptychadena anchietae (Bocage, 1868) and Upemba Grass Frog, Ptychadena upembae (Schmidt & Inger, 1959). These are followed by the families Hyperolidae with a series of 91 tadpoles and juveniles of an unidentified Reed Frog Hyperolius sp., two specimens of the Angolan Reed Frog Hyperolius angolensis Steindachner, 1867, and Phrynobatrachidae with 48 specimens of the Natal Dwarf Puddle Frog Phrynobatrachus natalensis (Smith, 1849). These three families make up almost 80% of the amphibian specimens. The reptile collection is taxonomically more diverse, comprising a total of 15 families corresponding to a total of 31 genera. Chelonians are represented by a single family, Pelomedusidae, while all other families are squamates. Among the squamates (non-snakes) the Scincidae is the best represented family with 124 specimens of four different genera, including Trachylepis (102 specimens), Sepsina (16 specimens), Panaspis (four specimens), and Lubuya (two specimens); followed by the family Agamidae with 51 specimens of two different genera, Acanthocercus (28 specimens) and Agama (23 specimens), and the families Chamaeleonidae (41 specimens) and Lacertidae (31 specimens), both represented by a single species, the Quilo Flap-Neck Chamaeleon, Chamaeleo dilepis quilensis Bocage, 1886 and the Angolan Rough-Scaled Lizard Ichnotropis bivittata Bocage, 1866, respectively. This collection includes a good diversity of snakes representative of the country, comprising six different families, Typhlopidae, Viperidae, Lamprophiidae, Elapidae, Colubridae, and Natricidae, representing 31% of the collection.

Overview of the Angola amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
ANURA Duméril, 1806
Pipidae Gray, 1825 Xenopus Wagler, 1827 Xenopus petersii Bocage, 1897 Calandula [or Kalandula] waterfalls – IICT/A 1/1957; Lucusse – IICT/A 18/1957; Calombe river – IICT/A 27/1959 Ruas (2002a) 3
Bufonidae (Gray, 1825) Sclerophrys Tschudi, 1838 Sclerophrys garmani (Meek, 1897) Calombe river – IICT/A 168/1959 Ruas (2002a) 1
Sclerophrys gutturalis (Power, 1927) Calombe river – IICT/A 155/1959, IICT/A 166/1959; Caluando river, Luando Reserve – IICT/A 66–67/1958; Dilolo lake – IICT/A 64–65/1958; Lubango – IICT/A 55–1957; Luena – IICT/A 14/1957, IICT/A 17/1957; Luvuei – IICT/A 20/1957 Ruas (2002a) 10
Sclerophrys pusilla (Mertens, 1937) Calandula [or Kalandula] waterfalls – IICT/A 2/1957 Ruas (2002a) 1
Sclerophrys sp. Calombe river – IICT/A 151/1959 Ruas (2002a) 1
Poyntonophrynus Frost, Grant, Faivovich, Bain, Haas, Haddad, de Sá, Channing, Wilkinson, Donnellan, Raxworthy, Campbell, Blotto, Moler, Drewes, Nussbaum, Lynch, Green & Wheeler, 2006 Poyntonophrynus kavangensis Poynton & Broadley, 1988 Calombe – IICT/A 156/1959 Ruas (2002a) 1
Brevicipitidae Bonaparte, 1850 Breviceps Merrem, 1820 Breviceps poweri Parker, 1936 Calombe – IICT/A 163/1959, 169/1959, 174–177/1959 Ruas (2002a) 6
Hemisotidae Cope, 1867 Hemisus Günther, 1859 Hemisus marmoratus (Peters, 1854) Calombe – IICT/A 150/1959, 171/1959 2
Hyperoliidae Laurent, 1943 Hyperolius Rapp, 1842 Hyperolius angolensis Steindachner, 1868 Caconda – IICT/A 54/1957; Cameia lake – IICT/A 10-1958 2
Hyperolius sp. Calombe – IICT/A 100–112/1959, 152/1959; Calombe river – IICT/A 28–84/1959; Caluando river, Luando Reserve – IICT/A 26/1959; Saurimo – IICT/A 6/1957; Angola [undetermined locality] – IICT/A 147/1959 91
Arthroleptidae Mivart, 1869 Arthroleptis Smith, 1849 Arthroleptis stenodactylus Pfeffer, 1893 Calombe – IICT/A 172–173/1959 Ruas (2002a) 2
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena anchietae (Bocage, 1868) Dilolo lake – IICT/A 62/1958 Ruas (2002a) 1
Ptychadena grandisonae Laurent, 1954 Calombe river – IICT/A 113/1959; Luena – IICT/A 15/1957 Ruas (2002a) 2
Ptychadena cf. mascareniensis (Duméril & Bibron, 1841) Cameia lake – IICT/A 2–8/1958, 15–37/1958; Caluando river, Luando Reserve – IICT/A 17–20/1959, 22–23/1959; Dilolo lake – IICT/A 55–61/1958; Kwanza river margins, Luando Reserve – IICT/A 16-1959 Ruas (2002a) 45
Ptychadena subpunctata (Bocage, 1866) Cameia lake – IICT/A 1/1958, 38–50/1958; Dilolo lake – IICT/A 53/1958, 63/1958 Ruas (2002a) 16
Ptychadena sp. Calandula [or Kalandula] waterfalls – IICT/A 7–13/1957, 11/1958; Calombe – IICT/A 160–162/1959; Cameia lake – IICT/A 52/1958; Luena – IICT/A 16/1957; Angola [undetermined locality] – IICT/A 3/5/1957, 53/1957 17
Ptychadena upembae (Schmidt & Inger, 1959) Cameia lake – IICT/A 14/1958 Ruas (2002a) 1
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena uzungwensis (Loveridge, 1932) Calombe – IICT/A 167/1959 Ruas (2002a) 1
Phrynobatrachidae Laurent, 1941 Phrynobatrachus Günther, 1862 Phrynobatrachus natalensis (Smith, 1849) Calombe river – IICT/A 144–145/1959; Camanongue – IICT/A 21–52/1957; Kwanza river source, Luando Reserve – IICT/A 1/1959, 10–13/1959; Kwanza river margins, Luando Reserve – IICT/A 2–9/1959, 21/1959; Ruas (2002a) 48
Pyxicephalidae Bonaparte, 1850 Amietia Dubois, 1987 Amietia angolensis (Bocage, 1866) Calombe – IICT/A 164–165/1959; Luena – IICT/A 21A/1957 Ruas (2002a) 3
Ranidae Batsch, 1796 Amnirana Dubois, 199 Amnirana darlingi (Boulenger, 1902) Calombe – IICT/A 154/1959, 157/1959, 159/1959; Angola [undetermined locality] – IICT/A – 148–149/1959 Ruas (2002a) 5
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 259
REPTILES
CHELONII Brongiart, 1800
Pelomedusidae Cope, 1868 Pelomedusa Wagler, 1830 Pelomedusa subrufa (Lacepède, 1788) Angola [undetermined locality] – IICT/R 4/1958 1
Pelusios Wagler, 1830 Pelusios bechuanicus FitzSimons, 1933 Angola [undetermined locality] – IICT/R 1/1958, 1A/1958, 2A/1958, 3/1958 4
SQUAMATA Oppel, 1811
Gekkonidae Gray, 1825 Hemidactylus Goldfuss, 1820 Hemidactylus longicephalus Bocage, 1873 Quizambil mines – IICT/R 1–3/1957 Manaças (1963); Ceríaco, Agarwal, Marques and Bauer (2020) 3
Hemidactylus nzingae Ceríaco, Agarwal, Marques & Bauer, 2020 Candande farm – IICT/R 4–5/1957 Manaças (1963); Ceríaco, Agarwal, Marques and Bauer (2020) 2
Amphisbaenidae Gray, 1825 Dalophia Gray, 1865 Dalophia angolensis Gans, 1976 Calombe, 7 km “west” from Luena – IICT/R 50/1959, 167/1959, 204/1959, 265/1959, 294–296/1959, 298–300/1959, 318/1959, 387/1959, 399/1959, 430/1959 Gans (1976) 16
Zygaspis Cope, 1885 Zygaspis nigra Broadley & Gans, 1969 Calombe, 7 km “west” from Luena – IICT/R 44/1959, 203/1959, 314–316/1959, 339A/1959 6
Varanidae Hardwicke & Gray, 1824 Varanus Merrem, 1820 Varanus niloticus (Linnaeus, 1766) Dilolo lake – IICT/R 108/1958 Manaças (1963) 1
Lacertidae Bonaparte, 1831 Ichnotropis Peters, 1854 Ichnotropis bivittata Bocage, 1866 Luena – IICT/R 136–137/1958; Luena (Santa Cruz farm) – IICT/R 229–231/1959; Cameia lake – IICT/R 5/1958; Calombe – IICT/R 58–60/1959, 79–81/1959, 244/1959, 252/1959, 267/1959, 277/1959, 279/1959, 320–323/1959, 339/1959, 374–375/1959, 410/1959, 420/1959, 437/1959; Angola [undetermined locality] – IICT/R Angola6–9 Manaças (1963) 31
Scincidae Cuvier, 1808 Lubuya Horton, 1972 Lubuya ivensii (Bocage, 1879) Calombe – IICT/R 48/1959, 239/1959 Manaças (1963) 2
Panaspis Cope, 1868 Panaspis sp. Calombe – IICT/R 268/1959, 276–277/1959, 288/1959 4
Sepsina Bocage, 1866 Sepsina angolensis Bocage, 1866 Calombe – IICT/R 47/1959, 79A–81A/1959, 200–201/1959, 264/1959, 386/1959, 409/1959, 416/1959, 431–432/1959, 436/1959, 438–439/1959, Angola1 16
Scincidae Cuvier, 1808 Trachylepis Fitzinger, 1843 Trachylepis bayonii (Bocage, 1872) Calombe – IICT/R 243/1959, 246/1959, 254–255/1959, 258/1959, 268–276/1959, 278/1959, 288/1959, 324–325/1959, 327–338/1959, 364–373/1959, 376–385/1959, 378A/1959, 385A/1959; 421–425/1959, 427–428/1959; Angola [undetermined locality] – IICT/R Angola18 Manaças (1963) 60
Trachylepis sp. Cameia lake – IICT/R 30–31/1958 2
Trachylepis wahlbergii (Peters, “1869” 1870) Kuito – IICT/R 77/1957, 81/1957; Cameia lake – IICT/R 23/1958, 26/1958, 232/1959; Calombe – IICT/R 61–67/1959, 253/1959, 256–257/1959, 259/1959, 280/1959, 283/1959, 326/1959, 335/1959, 405/1959, 426/1959; Dilolo lake – IICT/R 79–84/1959, 124–125/1958; Luena – IICT/R 234–235/1959, 238/1959; Santa Cruz farm, Luena – IICT/R 138/1958; Angola [undetermined locality] – IICT/R Angola10–16 Manaças (1963) 40
Gerrhosauridae Fitzinger, 1843 Gerrhosaurus Wiegmann, 1828 Gerrhosaurus bulsi Laurent, 1954 Cameia lake – IICT/R 122/1958; Dilolo lake – IICT/R 2/1958, 72/1958 Manaças (1963) 3
Tetradactylus Merrem, 1820 Tetradactylus ellenbergeri (Angel, 1922) Calombe – IICT/R 310–313/1959, 319/1959 5
Agamidae Gray, 1827 Acanthocercus Fitzinger, 1843 Acanthocercus cyanocephalus (Falk, 1925) Cachingues – IICT/R 83/1957; Cacolo – IICT/R 15–18/1959; Calombe – IICT/R 53–55/1959, 69/1959, 251/1959, 261/1959, 291/1959, 302/1959, 391/1959, 395/1959; Cassamba – IICT/R 40/1957; Luchazes – IICT/R 38/1957; Luena – IICT/R 28/1957, 33/1957; Lumbala Nguimbo – IICT/R 36–37/1957, Kuito – IICT/R 78/1957; Santa Cruz farm, Luena – IICT/R 133–135/1959, 139–141/1959 Manaças (1963) 28
Agama Daudin, 1802 Agama aculeata Merrem, 1820 Cameia lake – IICT/R 27–28/1958; Calombe – IICT/R 57/1959, 68/1959, 70/1959, 262–263/1959, 360–361/1959, 401/1959, 407/1959, 449/1959, 450A/1959; Cassamba – IICT/R 39-1957; Huambo – IICT/R 86/1957 Manaças (1963) 15
Agama shacki Mertens, 1938 Calandula [or Kalandula] waterfalls – IICT/R 32/1959, 32A/1959; outskirts of Huambo – IICT/R 84–85/1957 4
Agama sp. Kwanza river waterfalls – IICT/R 8–11/1957 Manaças (1963) 4
Chamaeleonidae Gray, 1825 Chamaeleo Laurenti, 1768 Chamaeleo dilepis Leach, 1819 Cacolo – IICT/R 19/1957; Cameia lake – IICT/R 24/1958, 74/1958; Calombe – IICT/R 56/1959, 71/1959, 354–356/1959, 396–397/1959, 402–403/1959, 408/1959, 411/1959, 413–414/1959, 451/1959, 454–455/1959; Dilolo lake – IICT/R 85–96/1958, 123/1958; Luena (Santa Cruz farm) – IICT/R 131 132/1958, 146/1958, 146A/1958, 149/1959, 149A/1959; Luena –IICT/R 42/1959, 241/1959; Mutumbo – IICT/R 82-1957 Manaças (1963 41
SERPENTES
Typhlopidae Merrem, 1820 Afrotyphlops Broadley & Wallach, 2009 Afrotyphlops angolensis (Bocage, 1866) Calombe Reserve – IICT/R 415A/1959, 416A/1959, 429/1959; Kwanza river source, Luando Reserve – IICT/R 34/1959 4
Afrotyphlops sp. Dundo – IICT/R Angola17 1
Viperidae Oppel, 1811 Bitis Gray, 1842 Bitis arietans (Merrem, 1820) Luena – IICT/R Angola5 1
Bitis gabonica (Duméril, Bibron & Duméril, 1854) Moxico – IICT/R A-1966 1
Viperidae Oppel, 1811 Causus Wagler, 1830 Causus bilineatus Boulenger, 1905 Cameia lake – IICT/R 12A/1958; Calombe – IICT/R 249/1959 Manaças (1973 “1974”) 2
Causus rhombeatus (Lichtenstein, 1823) Calombe – IICT/R 151–152/1958, 51/1959, 52A/1959, 209/1959, 290/1959, 304/1959, 306/1959, 309/1959, 386A/1959, 433/1959, 453/1959, 479/1959; Camanongue – IICT/R 75/1957; Cameia lake – IICT/R 75/1958; Cazombe river source – IICT/R 52/1959; Dilolo lake – IICT/R 126/1958; Luena (Santa Cruz farm) – IICT/R 145/1958; Luena – IICT/R 8/1959, Angola4, Angola19; Angola [undetermined locality] – IICT/R 28/1959, 31/1959, 33A/1959, 38/1959, 345/1959, 384A/1959, 410B/1959, 437A/1959, 438A/1959, 441/1959 Manaças (1973 “1974”) 31
Lamprophiidae Fitzinger, 1843 Boaedon Duméril, Bibron & Duméril, 1854 Boaedon fradei Hallermann et al. 2020 Calombe – IICT/R 150/1958, 5/1959, 40/1959, 341/1959, 350/1959, 400/1959, 404/1959, 410A/1959, 450/1959, 340/1968; Luena – IICT/R 240/1968 Manaças (1973 “1974”); Hallermannn et al. (2020) 11
Lycophidion Fitzinger, 1843 Lycophidion capense (Smith, 1831) Calombe – IICT/R 292/1959, 448/1959 2
Psammophis Boie, 1825 Psammophis angolensis (Bocage, 1872) Cameia lake – IICT/R 7/1958 1
Psammophis mossambicus Peters, 1882 Calombe – IICT/R 29/1959, 75/1959, 77/1959, 18/1959, 245/1959, 431A/1959, 443/1959, 452/1959; Cameia National Park – IICT/R 77/1958; Dilolo lake – IICT/R 127/1958; Luena – IICT/R Angola20; Angola [undetermined locality] – IICT/R 12/1958, 14/1958 Manaças (1973 “1974”) 13
Psammophis zambiensis Hughes & Wade, 2002 Calombe – IICT/R 303/1959; Cameia lake – IICT/R 30A/1958 Manaças (1973 “1974”) 2
Psammophylax acutus (Günther, 1888) Calombe – IICT/R 210–227/1959, 285/1959, 434/1959, 444/1959 Manaças (1973 “1974”) 21
Psammophylax sp. Menongue – IICT/R 15/1958 1
Prosymna Gray, 1849 Prosymna angolensis Boulenger, 1915 Milando – IICT/R 14/1957; Angola [undetermined locality] – IICT/R 348–349, 348–349/1959 5
Elapidae Boie, 1827 Pseudohaje Günther, 1858 Pseudohaje goldii (Boulenger, 1895) Luachimo dam canal – IICT/R Angola3 1
Colubridae Oppel, 1811 Crotaphopeltis Fitzinger, 1843 Crotaphopeltis barotseensis Broadley, 1968 Dilolo lake – IICT/R 362/1959 1
Crotaphoepltis hotamboeia (Laurenti, 1768) Calombe – IICT/R 109–110/1958; Dilolo lake – IICT/R 362/1959, 406/1959, 417/1959 Manaças (1973 “1974”) 4
Dasypeltis Wagler, 1830 Dasypeltis scabra (Linnaeus, 1758) Calombe – IICT/R 447/1959 Manaças (1973 “1974”) 1
Dipsadoboa Günther, 1858 Dipsadoboa shrevei (Loveridge, 1932) Calombe – IICT/R 4/1959 1
Dispholidus Duvernoy, 1832 Dispholidus typus typus (Smith, 1828) Calombe – IICT/R 76/1959, 457–458/1959; Luena (Santa Cruz farm) – IICT/R 142/1958, 147–148/1958; Luena – IICT/R 386/1958, 1–2/1959, 386/1968 Manaças (1973 “1974”) 9
Dispholidus typus punctatus Laurent, 1955 Calombe – IICT/R 76/1959 Manaças (1973 “1974”) 1
Colubridae Oppel, 1811 Philothamnus Smith, 1840 Philothamnus angolensis Bocage, 1882 Candande farm – IICT/R 7/1957; Kuito – IICT/R 79/1957 Manaças (1973 “1974”) 2
Philothamnus heterolepidotus (Günther, 1863) Calombe – IICT/R 78/1958; Dilolo lake – IICT/R 121/1958, 130/1958; Lungué-Bungo river [or Lungwebungu] margins – IICT/R 41/1957, 247/1957; Uíge – IICT/R 17/1958 Manaças (1973 “1974”) 6
Natricidae Bomaparte, 1838 Limnophis Günther, 1865 Limnophis bangweolicus (Mertens, 1936) Cameia lake – IICT/R 25/1958; Dilolo lake – IICT/R 107/1958, 251/1958 3
Limnophis bicolor Günther, 1868 Calombe – IICT/R 33/1959, 45/1959; Luena – IICT/R 37/1959; Forest patch, Luena – IICT/R 35/1959 4
Natriciteres Loveridge, 1953 Natriciteres sp. Angola [undetermined locality] – IICT/R 16/1958 1
TOTAL NUMBER OF REPTILES SPECIMENS 418

The geographic range of this collection covers 38 different localities from 10 different provinces: Bengo, Bié, Cuando-Cubango, Huambo, Huíla, Lunda Norte, Lunda Sul, Malanje, Moxico, and Uíge (Table 8; Fig. 12). Moxico is the best represented province, with 574 records from 14 different localities, while Lunda Sul is the least sampled with only one record. Collecting events took place between 1957 and 1968, although most of the material was collected between 1957 and 1959 during the Apiary research mission to Angola organised by the CZL, especially aimed at the study of bees and honey production.

Gazetteer of Angola localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Province Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Bengo Minas do Quizambil Quizambil mines -8.3166667, 13.733333 301 262 1/3
Bié Cachingues Cachingues -13.071851, 16.749528 954 1713 1/1
Bié Mutumbo Mutumbo -13.183333, 17.4 430 1452 1/1
Bié Silva Porto Kuito -12.391968, 16.93867 2060 1716 3/4
Cuando Cubango Menongue? Menongue -14.666667, 17.7 3060 1378 1/1
Huambo Nova Lisboa [or arredores de Nova Lisboa] Huambo [or outskirts of Huambo] -12.766667, 15.733333 5000 1719 2/3
Huíla Colonata de Caconda Caconda -13.735583, 15.066912 1600 1670 1/1
Huíla Sá da Bandeira Lubango -14.916667, 13.5 6100 1751 1/1
Lunda Norte Cacolo, Mennungo [or Cacolo, Minungo] Cacolo -10.14172, 19.267969 1832 1351 2/5
Lunda Norte Canal da Barragem de Luachimo Luachimo Dam canal -7.361733, 20.84899 980 643 1/1
Lunda Norte Dundo Dundo -7.377554, 20.835054 2240 731 1/1
Lunda Norte Vila Henrique Carvalho Saurimo -9.662523, 20.39485 5000 1075 1/1
Malanje Quedas do Duque de Bragança Calandula [or Kalandula] waterfalls -9.073248, 16.001402 800 1064 4/12
Malanje Posto do Milando Milando -8.8166667, 17.566667 600 666 1/1
Moxico Calombe-Luso, Moxico Calombe -11.833333, 19.933333 3060 1345 37/249
Moxico Calombe (7 km de Vila Luso) Calombe, 7 km “west” from Luena -11.833333, 19.933333 3060 1345 2/20
Moxico Cameia Cameia National Park -11.883333, 21.666667 10000 1092 1/1
Moxico Cascata do Rio Cuanza, Cangandala Kwanza River waterfall -9.874712, 16.670194 3060 1086 1/4
Moxico Cassamba Cassamba -13.093312, 20.346395 2500 1345 2/2
Moxico Fazenda Santa Cruz, Luso Santa Cruz farm, Luena -11.783333, 19.916667 3060 1337 6/19
Moxico Lago Cameia Cameia lake -11.716667, 20.8 980 1110 16/63
Moxico Lago Dilolo Dilolo lake -11.5, 22.016667 4350 1088 14/44
Moxico Lucusse Lucusse -12.526722, 20.818813 1758 1144 1/1
Moxico Vila Gago Coutinho Lumbala Nguimbo -14.101654, 21.435308 2051 1122 1/2
Moxico Luvuei Luvuei -13.066667, 21.166667 1132 1136 1/1
Moxico Margens do Rio Lungué Lungué-Bungo river [or Lungwebungu] margins -12.45, 20.05 1300 1184 1/2
Moxico Mata da Horta dos Padres, Luso Horta dos Padres woods, Luena -11.783333, 19.916667 3060 1337 1/1
Moxico Moxico Moxico [undetermined locality] – – – 1/1
Moxico Nascente do Rio Cazombe, Luso Cazombe river source -11.883333, 22.916667 3060 1116 1/1
Moxico Vila Luso [or Luso]; Luena -11.783333, 19.916667 3060 1337 14/26
Moxico Polígono Florestal, Luso Forest patch, Luena -11.783333, 19.916667 10000 1330 1/1
Moxico Posto do Bussaco Camanongue -11.433333, 20.166667 2000 1258 2/33
Moxico Reserva da Palanca Preta, Nascente do Rio Quanza Kwanza river source, Luando Reserve [or Kwanza river margins] -11.116667, 17.46666667 3060 1191 2/14
Moxico Rio Caluando (Reserva da Palanca Preta) Caluando river, Luando Reserve -11.126218, 17.504279 5000 1174 4/10
Moxico Rio Calombe [Rio Calombe (Reserva da Palanca Preta)] Calombe river -11.833333, 19.916667 1370 1349 9/96
Moxico Serra Luchazes Luchazes -13.9166667, 20.8 600 1271 1/1
Uíge Fazenda Otília, Enconge Candande Farm -7.55, 15.03 980 763 2/3
Uíge Uíge Uíge -7.6166667, 15.05 4829 825 1/1
– Angola Angola [undetermined locality] – – – 12/41
10.3897/zookeys.1052.64607.figure12 5168174 EB28C751-73BF-5758-BFE7-ECB938353999

Distribution of the Angola localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572780

Part of the snake and lizard collections was studied and published by Manaças (1963, 1973 “1974”, 1982), while the amphibians were studied by Ruas (1996, 2002a). In 1976 the American herpetologist Carls Gans (1923–2009) described a new species of amphisbaenian, the Angolan Worm Lizard, Dalophia angolensis, based on a series of specimens from this collection (Gans 1976). Several other well-known herpetologists such as Donald G. Broadley (1932–2016) and Barry Hughes (1935–to date) used the collection for their own research and provided important contributions to specimen identification. More recently the collection has been used in several projects, including new species descriptions (Ceríaco et al. 2020a, b; Hallermannn et al. 2020).

The collection holds type material for several species: Dalophia angolensis Gans, 1976 (holotype and 18 paratypes); Queen Nzinga’s Tropical Gecko, Hemidactylus nzingae Ceríaco, Agarwal, Marques & Bauer, 2020 (two paratypes); and Frade’s Brown House Snake, Boaedon fradei Hallermannn, Ceríaco, Schmitz, Ernst Conradie, Verburgt, Marques & Bauer, 2020 (five paratypes). The IICT holds one of the largest known herpetological collections from Angola, with 677 specimens. Currently, the largest collection of Angolan amphibians and reptiles is held by MD, with a total of 2753 specimens (Ceríaco et al. 2020d), followed by the collections of AMNH with a total of 2280 specimens, the CAS with 1347 specimens, the TM with 935 specimens, and the CM with 806 specimens (data retrieved from GBIF.org in February 2021). Other collections, as is the case of PEM in South Africa, ISCED and INBAC in Angola, have considerable and comparable collections, but the data regarding these collections is not available on online databases.

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018c; https://www.gbif.org/dataset/ae52efd5-bfd4-4a81-ba12-813cace064f3).

 Mozambique collection

Mozambique has a high diversity of amphibians and reptiles but remains relatively unexplored when compared with other southern African regions (Pietersen 2014). Since Wilhelm Peters’ (1815–1883) historical survey and the publication of his two major works (Peters 1854, 1882), very few surveys have been conducted in the country. The scientific literature of Mozambican herpetofauna was mostly incorporated on comprehensive works about southern and eastern African herpetofauna (e.g., FitzSimons 1962; Broadley 1990a; Channing 2001; Du Preez and Carruthers 2009; Spawls et al. 2018). In the last decade Mozambique has been targeted by researchers and new fieldwork is being conducted. This has already resulted in important contributions and new species descriptions (e.g., Branch et al. 2005a, 2014, 2017; Branch and Bayliss 2009; Branch and Tolley 2010; Portik et al. 2013a; Conradie et al. 2016) and checklists (Broadley 1990b, 1992; Branch et al. 2005b; Schneider et al. 2005; Downs and Wirminghaus 2010; Jacobsen et al. 2010; Pietersen et al. 2013; Ohler and Frétey 2014; Pietersen 2014; Weinell et al. 2017). Currently, the Mozambican herpetofauna comprises more than 100 amphibians and 294 reptiles (Frost 2021; Bates 2018).

The collection comprises 1181 specimens (532 amphibians and 649 reptiles), 26 species of amphibians, and 70 species of reptiles and two subspecies (Table 9), corresponding to approximately 26% of the known taxa for the country. The amphibian collection comprises representatives of 11 families and 16 genera, all anurans. The families Bufonidae, Pyxicephalidae, and Ptychadenidae are the best represented with 193, 125, and 85 specimens, respectively. The reptile collection comprises representatives of 16 families and 50 genera, all squamates, with the exception of four juvenile specimens of Crocodylus niloticus Laurenti, 1768. The families Scincidae, Agamidae, and Chamaeleonidae are the best represented in the collection with representatives of all taxa known from the country with 199, 127, and 83 specimens, respectively. Lamprophiidae is the family with the greatest diversity of species (13), followed by the Scincidae (10) and Colubridae (9).

Overview of the Mozambique amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
ANURA Duméril, 1806
Pipidae Gray, 1825 Xenopus Wagler, 1827 Xenopus muelleri (Peters, 1844) Porto Henrique – IICT/A 1–9/1948, 10–19/1948; Chizizira – IICT/A 1/1965; Gorongosa – IICT/A 44/1955, 49–54/1955, 69–76/1955, 85–90/1955; Caniçado, B.T.L. vivarium – IICT/A 42–42/1963, 278–283/1955; Mozambique [undetermined locality] – IICT/A 38–39/1966 Manaças (1950); Ruas (2002b) 50
Bufonidae (Gray, 1825) Schismaderma Smith, 1849 Schismaderma carens (Smith, 1848) Guenguela stream, Chibuto – IICT/A 185–189/1948, 209/1948; Matola – IICT/A 252–255/1948; Moamba – IICT/A 171–179/1948 Manaças (1950), Ruas (2002b) 19
Sclerophrys Tschudi, 1838 Sclerophrys garmani (Meek, 1897) Boane – IICT/A 10/1963, 21/1963, 35/1948, 38/1948, 59/1948, 61/1948, 67/1948, 69/1948; Caniçado, watering channel – IICT/A 24–32/1963, 89/1963, 91/1963, 103/1963, 106/1963; Caniçado – IICT/A 60/1963; Centro Social – IICT/A 114/1963; Guenguela stream, Chibuto – IICT/A 190–215/1948; Guijá – IICT/A 81–83/1963; Manhiça – IICT/A 257/1948; Maqueze – IICT/A 239–245/1948; Moamba – IICT/A 180/1948; Mucomaze – IICT/A 22–33/1948; Porto Henrique – IICT/A 39/1948, 286/1948; Lagoa de S. Matinho – IICT/A 315–318/1955 Mozambique [undetermined locality] – IICT/A Moz3 Manaças (1950); Ruas (2002b) 81
Sclerophrys gutturalis (Power, 1927) Centro Social – IICT/A 5/1964; Chizizira – IICT/A 18–20/1966; Dondo – IICT/A 163–166/1948; Gorongosa – IICT/A 7–19/1955, 78/1955, 82–83/1955, 95–96/1955, 118–123/1955, 144–147/1955, 152–156/1955, 171–175/1955; Guijá – IICT/A 80/1963; Mauele – IICT/A 79–102/1948; Sussundenga – IICT/A 30–31/1966, 36/1966; Mozambique [undetermined locality] – IICT/A 67A/1948, 309–315/1955, Moz4 Manaças (1950); Ruas (2002b) 83
Sclerophrys pusilla (Mertens, 1937) Chizizira – IICT/A 33–34/1966; Gorongosa – IICT/A 97–100/1955, 157/1955 Ruas (2002b) 7
Sclerophrys sp. Licungo river – IICT/A Moz1; Mozambique [undetermined locality] – IICT/A 308/1955 3
Microhylidae Günther, 1858 (1843) Phrynomantis Peters, 1867 Phrynomantis bifasciatus (Smith, 1847) Lagoa Chicungue, Bilene – IICT/A 320/1955 Ruas (2002b) 1
Brevicipitidae Bonaparte, 1850 Breviceps Merrem, 1820 Breviceps mossambicus Peters, 1854 Manhiça – IICT/A 68/1948; Ponta do Ouro – IICT/A 34/1948 Manaças (1950); Ruas (2002b) 2
Hemisotidae Cope, 1867 Hemisus Günther, 1859 Hemisus marmoratus (Peters, 1854) Mozambique [undetermined locality] – IICT/A 258/1948 Ruas (2002b) 1
Hyperoliidae Laurent, 1943 Hyperolius Rapp, 1842 Hyperolius marmoratus Rapp, 1842 Chimoio – IICT/A 74–75/1948; Manhiça – IICT/A 249–250/ 1948, 54–57/1948, 72/1948; Gorongosa – IICT/A 20/1955; Mozambique [undetermined locality] – IICT/A 298/1955, Moz2 Manaças (1950) 12
Hyperolius sp. Maluana – IICT/A 69/1963; Gorongosa – IICT/A 45/1955 2
Hyperolius tuberilinguis Smith, 1849 Boane – IICT/A 22/1963; Chimoio – IICT/A 25–29/1966; Ulongué – IICT/A 162/1948; Gorongosa – IICT/A 48/1955 8
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena guibei Laurent, 1954 Gorongosa – IICT/A 55/1955 1
Ptychadena mascareniensis (Duméril & Bibron, 1841) Manhiça – IICT/A 62–63/1948; Magaiza, Panda – IICT/A 246/1948; Mauele – IICT/A 103–140/1948, 142–161/1948; Pafuri – IICT/A 77/1948; Mozambique [undetermined locality] – IICT/A 289/1955 Manaças (1950); Ruas (2002b) 63
Ptychadenidae Dubois, 1987 Ptychadena Boulenger, 1917 Ptychadena oxyrhynchus (Smith, 1849) Gorongosa – IICT/A 56–68/1955, 133–137/1955, 161/1955 Ruas (2002b) 19
Ptychadena taenioscelis Laurent, 1954 Mucomaze – IICT/A 21/1948; Gorongosa – IICT/A 295/1955 Ruas (2002b) 2
Phrynobatrachidae Laurent, 1941 Phrynobatrachus Günther, 1862 Phrynobatrachus mababiensis FitzSimons, 1932 Pafuri – IICT/A 76/1948, 78/1948; Umbeluzi – IICT/A 297/1955 Ruas (2002b) 3
Phrynobatrachus natalensis (Smith, 1849) Namaacha – IICT/A 41/1948, 43/1948, 45/1948, 49–52/1948; Luá (Ile) river – IICT/A 1–2/1955 Ruas (2002b) 12
Pyxicephalidae Bonaparte, 1850 Amietia Dubois, 1987 Amietia angolensis (Bocage, 1866) Gorongosa – IICT/A 3–6/1955, 21–41/1955, 47/1955, 91–94/1955, 101–131/1955, 138–143/1955, 148–151/1955, 158–160/1955, 167–170/1955, 272–275/1955, 284–28871955; Mozambique [undetermined locality] – IICT/A 29A/1955, Moz5 Ruas (2002b) 83
Cacosternum Boulenger, 1887 Cacosternum cf. boettgeri (Boulenger, 1882) Gorongosa Mount – IICT/A 225/1955 Ruas (2002b) 1
Cacosternum cf. nanum (Boulenger, 1887) Gorongosa – IICT/A 42/1955 Ruas (2002b) 1
Pyxicephalus Tschudi, 1838 Pyxicephalus edulis Peters, 1854 Caniçado – IICT/A 90/1963, 92–93/1963; Caniçado, watering channel – IICT/A 101–109/1963; Chibuto – IICT/A 181–182/1948; Namaacha – IICT/A 40/1948; Mozambique [undetermined locality] – 286A/1955 Ruas (2002b) 14
Strongylopus Tschudi, 1838 Strongylopus rhodesianus (Hewitt, 1933) Gorongosa Mount – IICT/A 176–197/1955 Ruas (2002b) 22
Tomopterna Duméril & Bibron, 1841 Tomopterna marmorata Peters, 1854 Manhiça – IICT/A 60/1948, 256/1948 Ruas (2002b) 2
Ranidae Batsch, 1796 Amnirana Dubois, 199 Amnirana darlingi (Boulenger, 1902) Umbeluzi – IICT/A 291–294/1955 Ruas (2002b) 4
Rhacophoridae Hoffman, 1932 [1858] Chiromantis Peters, 1854 Chiromantis xerampelina Peters, 1854 Chicualacuala – IICT/A 73/1948; Enchisa – IICT/A 20/1948; Manhiça – IICT/A 251/1948; Maqueze – IICT/A 216–232/1948, 234–238/1948; Limpopo river margins – IICT/A 61–66/1963, 74/1963; Moamba – IICT/A 168–170/1948 Manaças (1950); Ruas (2002b) 35
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 532
REPTILES
CROCODYLIA Gmelin, 1789
Crocodylidae Cuvier, 1808 Crocodylus Laurenti, 1768 Crocodylus niloticus Laurenti, 1768 Nhaluiro – IICT/R 13–16/1971 4
SQUAMATA Oppel, 1811
Gekkonidae Gray, 1825 Afroedura Loveridge, 1944 Afroedura loveridgei Broadley, 1963 Viola, Mazowe River Bridge – IICT/R/DB UM 3992 Broadley (1963) 1
Chondrodactylus Peters, 1870 Chondrodactylus turneri (Gray, 1864) Guenguela stream, Chibuto – IICT/R 1345/1948; Enchisa – IICT/R 178/1948; Magaiza, Panda – IICT/R 1638–1640/1948 Manaças (1952) 5
Hemidactylus Goldfuss, 1820 Hemidactylus mabouia (Moreau de Jonnès, 1818) Chibuto – IICT/R 1320–1322/1948; Mauele – IICT/R 1582/1952; Nhaluiro – IICT/R 8–10/1971; Gorongosa – IICT/R 114/1955, 148–149/1955 Manaças (1952) 10
Homopholis Boulenger, 1885 Homopholis wahlbergii (Smith, 1849) Homoíne – IICT/R 111/1963 1
Lygodactylus Gray, 1864 Lygodactylus capensis (Smith, 1849) Gorongosa – IICT/R 7–8/1955, 14/1955, 16/1955, 115/1955, 146–149/1955, 153A/1955; Lifidzi, Angónia – IICT/R 132A/1948, 1782–1783/1948; Manhiça – IICT/R 644–646/1948; Manaças (1952, 1961) 16
Amphisbaenidae Gray, 1825 Monopeltis Smith, 1848 Monopeltis sphenorhynchus (Peters, 1879) Manhiça – IICT/R 532–538/1948 Manaças (1954) 8
Zygaspis Cope, 1885 Zygaspis violacea (Peters, 1854) Manhiça – IICT/R 524–531/1948, 1747/1948 Manaças (1954) 9
Varanidae Hardwicke & Gray, 1824 Varanus Merrem, 1820 Varanus exanthematicus (Bosc, 1792) Caniçado – IICT/R 39-1963 1
Lacertidae Bonaparte, 1831 Heliobolus Fitzinger, 1843 Heliobolus lugubris (Smith, 1938) Pafuri – IICT/R 727/1948 Manaças (1961) 1
Nucras Gray, 1838 Nucras cf. holubi (Steindachner, 1882) Boane – IICT/R 52/1963; Gorongosa – IICT/R 36/1955, 131/1955, 137–138/1955; Lifidzi, Angónia – IICT/R 1792–1795/1948; Namaacha – IICT/R 400/1948 Manaças (1952, 1961 10
Scincidae Cuvier, 1808 Acontias Cuvier, 1816 [1817] Acontias aurantiacus (Peters, 1854) Mauele – IICT/R 1023/1948, 1151–1152/1948, 1196/1948, 1199/1948 Manaças (1954) 5
Mochlus Günther, 1864 Mochlus sundevalli (Smith, 1849) Mauele – IICT/R 1164–1167/1948, 1581/1948; Gorongosa – IICT/R 28/1955 Manaças (1952, 1961) 6
Panaspis Cope, 1868 Panaspis wahlbergii (Smith, 1849) Lifidzi, Angónia – IICT/R 1790–1791/1948 Manaças (1952) 2
Scelotes Fitzinger, 1826 Scelotes arenicola (Peters, 1854) Mauele – IICT/R 1153–1156/1948, 1200–1202/1948 Manaças (1954) 7
Trachylepis Fitzinger, 1843 Trachylepis boulengeri (Sternfeld, 1911) Gorongosa – IICT/R 116/1955 1
Trachylepis depressa (Peters, 1854) Mauele – IICT/R 1584/1948 Manaças (1952) 1
Trachylepis margaritifera (Peters, 1854) Nhandare river margins, Gorongosa – IICT/R 10–13/1955, 13A/1955; Nhandare river, Gorongosa – IICT/R 30–31/1955; Namaacha – IICT/R 348/1948, 414–415/1948, 441–449/1948; Nhaluiro – IICT/R 4/1971, 7/1971; Sussundenga – IICT/R 10–11/1955 Manaças (1952, 1961) 23
Trachylepis sp. Mauele – IICT/R 1582/1948; Porto Henrique – IICT/R 235/1948 2
Trachylepis striata (Peters, 1844) Boane – IICT/R 14/1963, 16/1963, 45/1963, 51/1963, 53–54/1963; Caniçado, watering channel – IICT/R 59/1963; Chibuto, Ribeira de Guenguela – IICT/R 1354–1357/1948; Dondo – IICT/R 911/1948; Lifidzi, Angónia – IICT/R 132C/1948; Limpopo river Dam – IICT/R 33–34/1963; Manhiça – IICT/R 561–613/1948, 1692–1694/1948, 1719/1948, 1744/1948, 1962–1964/1948; Maqueze, Alto Changane – IICT/R 1504–1515/1948; Mauele – IICT/R 1024–1032/1948, 1160–1162/1948, 1184–1192/1948; Moamba – IICT/R 1297–1298/1948, 1323/1948; Mutuali – IICT/R Moz10; Nhaluiro – IICT/R 1–3/1971, 5–6/1971; São Martinho do Bilene – IICT/R 178/1955; road between Inhassoro and Vilanculos – IICT/R 2/1955; Sussundenga – IICT/R 17/1964; Umbeluzi – IICT/R 1–6/1963; Gorongosa – IICT/R 32–33/1955, 87–92/1955 Manaças (1952, 1961) 135
Trachylepis varia (Peters, 1867) Enchisa – IICT/R 179/1948; Gorongosa Mount – IICT/R 144/1955; Gorongosa – IICT/R 34–35/1955, 117/1955, 144/1955; Lifidzi, Angónia – IICT/R 1789/1948, 1789A/1948; Mauele – IICT/R 1163/1948, 1588/1948; Namaacha – IICT/R 417/1948 Manaças (1952, 1961) 17
Cordylidae Mertens, 1937 Smaug Stanley, Bauer, Jackman, Branch & Mouton (2011) Smaug mossambicus (FitzSimons, 1958) Gorongosa – IICT/R 29/1955 1
Cordylidae Mertens, 1937 Platysaurus Smith, 1844 Platysaurus intermedius wilhelmi Hewitt, 1909 Namaacha – IICT/R 418–420/1948, 450–454/1948; 15 mls SE of Manica – IICT/R/DB UM 3579 Manaças (1952) 9
Platysaurus maculatus lineicauda Broadley, 1965 14 mls west of Morrumbala – IICT/R/DB UM 7981 Broadley (1965) 1
Platysaurus maculatus maculatus Broadley, 1965 Mitucué Mountains – IICT/R/DB UM 8064, 8071 Broadley (1965) 2
Gerrhosauridae Fitzinger, 1843 Gerrhosaurus Wiegmann, 1828 Gerrhosaurus flavigularis Wiegmann, 1828 Gorongosa – IICT/R 59–60/1955; 124/1955, 127/1955, 156/1955; Manhiça – IICT/R 1691/1948; Mutuali – IICT/R 1/1953; Mozambique [undetermined locality] – IICT/R Moz12 Manaças (1952, 1961) 8
Matobosaurus Bates & Tolley, 2013 Matobosaurus validus (Smith, 1849) Centro Social – IICT/R 6/1965; Gorongosa – IICT/R 39/1955, 121/1955; Nhandare river margins, Gorongosa – IICT/R 9/1955; Namaacha – IICT/R 416/1948 Manaças (1961) 5
Tetradactylus Merrem, 1820 Tetradactylus cf. ellenbergeri (Angel, 1922) Meponda – IICT/R Moz6 2
Agamidae Gray, 1827 Acanthocercus Fitzinger, 1843 Acanthocercus atricollis (Smith, 1849) Boane – IICT/R 17/1963; Caniçado, B.T.L. vivarium – IICT/R 69–70/1963, Moz16; Guenguela stream, Chibuto – IICT/R 1326–1344/1948, 1429–1436/1948; Guijá – IICT/R 964–966/1948; Inharrime – IICT/R 110/1963; Manhiça – IICT/R 553–560/1948; Maqueze, Alto Changane – IICT/R 1499–1500/1948; Limpopo river margins – IICT/R 79/1963; Namaacha – IICT/R 349–413/1948; Sussundenga – IICT/R 39/1966 Manaças (1952) 57
Agama Daudin, 1802 Agama armata Peters, 1855 Lifidzi, Angónia – IICT/R 132B/1948, 1784–1788/1948; Manhiça – IICT/R 1695/1948; Mauele – IICT/R 1204–1212/1948; Sussundenga – IICT/R 16–22/1966 Manaças (1952) 20
Agama mossambica Peters, 1854 Centro Social – IICT/R 7/1964; Dondo – IICT/R 898–904/1948; Gorongosa – IICT/R 5–6/1955, 18–26/1955, 61/1955, 64–70/1955, 93–97/1955, 111–113/1955, 128–129/1955, 132–134/1955, 139–141/1955 Manaças (1961) 41
Agama kirkii Boulenger, 1885 Centro Social – IICT/R 8–9/1964; Sussundenga IICT/R 2–4/1964, 12–15/1966 9
Chamaeleonidae Gray, 1825 Chamaeleo Laurenti, 1768 Chamaeleo dilepis Leach, 1819 Boane – IICT/R 12–13/1963, 15/1963; Caniçado IICT/R 67–68/1963, 84–85/1963, 95–97/1963; Gorongosa – IICT/R 471955, 27/1955, 71–80/1955, 81A/1955, 82/1955, 98–99/1955, 100–102/1955, 108–110/1955, 122/1955, 125–126/1955, 161–165/1955, 166–167/1955; Guenguela stream, Chibuto – IICT/R 1346–1353/1948, 1354A/1948, 1356A/1948, 1426–1428/1948, 1439–1440/1948; Guijá – IICT/R 37–38/1948, 58/1963, 71/1963, 115/1963; Lifidzi, Angónia – IICT/R 1796–1800/1948; Manhiça IICT/R 1801/1948, 614/1948, 616–620/1948, 642/1948; Matola – IICT/R 20/1963; Mocuba – IICT/R 3A/1955; São Martinho do Bilene – IICT/R 174–177/1955 Manaças (1952, 1961) 81
Chamaeleo gracilis Hallowell, 1844 Mozambique [undetermined locality] – IICT/R Moz17 1
Rhampholeon Günther, 1874 Rhampholeon gorongosae Broadley, 1971 Gorongosa – IICT/R 145/1955 1
SERPENTES
Typhlopidae Merrem, 1820 Afrotyphlops Broadley & Wallach, 2009 Afrotyphlops cf. fornasinii (Bianconi, 1849) Mauele – IICT/R 1157–1159/1948 3
Afrotyphlops cf. mucruso (Peters, 1854) Chizizira – IICT/R 38/1966; Nhaluiro – IICT/R 1A/1971; Pafuri – IICT/R Moz26 3
Afrotyphlops cf. schlegelii (Bianconi, 1849) Meponda – IICT/R Moz5; Nova Mambone – IICT/R Moz3; presumably Mozambique [undetermined locality] – IICT/R Moz25 3
Leptotyphlops Fitzinger, 1843 Leptotyphlops incongnitus (Broadley & Watson, 1976) Cafumpe – IICT/R 63/1948 Manaças (1954) 1
Viperidae Oppel, 1811 Bitis Gray, 1842 Bitis arietans (Merrem, 1820) Gorongosa – IICT/R 17/1955, 40A/1955, 43–44/1955, 150/1955; Govuro IICT/R 5/1956; Manhiça IICT/R 797/1948; Tchizigine – IICT/R 10/1964; Ulongué – IICT/R 103/1948; Mozambique [undetermined locality] – IICT/R Moz7, Moz8 Manaças (1959) 11
Causus Wagler, 1830 Causus defilippii (Jan, 1863) Chimoio – IICT/R 35/1966; Gorongosa – IICT/R 49/1955, 82–83/1955 Manaças (1959) 4
Causus cf. rhombeatus (Lichtenstein, 1823) Metengo Balama – IICT/R 2/1949 Manaças (1959) 1
Lamprophiidae Fitzinger, 1843 Amblyodipsas Peters, 1857 Amblyodipsas microphthalma (Bianconi, 1852) Mauele – IICT/R 948/1948, 1150/1948 Manaças (1959) 2
Amblyodipsas polylepis (Bocage, 1873) Nova Mambone – IICT/R 2/1956 1
Aparallactus Smith, 1849 Aparallactus capensis Smith, 1849 Mauele – IICT/R 1012/1948, 1194/1948; Sussundenga – IICT/R 16/1964; Porto Henrique – IICT/R 1642/1948 4
Aparallactus lunulatus (Peters, 1854) Gorongosa – IICT/R 54/1955 Manaças (1959) 1
Atractaspis Smith, 1849 Atractaspis bibronii Smith, 1849 Gorongosa – IICT/R 57/1955 1
Boaedon Duméril, Bibron & Duméril, 1854 Boaedon capensis Duméril, Bibron & Duméril, 1854 Boane – IICT/R 8–9/1963; Manhiça – IICT/R 1682/1948, 1684/1948, 1687/1948; Mauele – IICT/R 1248/1948; Namaacha – IICT/R 460/1948; Gorongosa – IICT/R 62/1955, 106/1955, 143/1955; Ulongué – IICT/R 1/1947; Mozambique [undetermined locality] – IICT/R 40/1967 Manaças (1959) 12
Boaedon sp. Pafuri – IICT/R Moz11 1
Limaformosa Broadley, Tolley, Conradie, Wishart, Trape, Burger, Kusamba, Zassi-Boulou & Greenbaum, 2018 Limaformosa capensis (Smith, 1847) Chibuto – IICT/R 1425/1948 Manaças (1959) 1
Lycophidion Fitzinger, 1843 Lycophidion capense (Smith, 1831) Gorongosa – IICT/R 56/1955 Manaças (1959) 1
Psammophis Boie, 1825 Psammophis mossambicus Peters, 1882 Boane – IICT/R Moz1; Gorongosa – IICT/R 47/1955; Manhiça – IICT/R 1685–1686/1948; Maputo – IICT/R 241/1948; Mauele – IICT/R 157/1948, 1015/1948; Nova Mambone – IICT/R 7/1955; São Martinho do Bilene – IICT/R Moz 14–15; Mozambique [undetermined locality] – IICT/R Moz9, 21–23 Manaças (1959) 14
Lamprophiidae Fitzinger, 1843 Psammophis Boie, 1825 Psammophis orientalis Broadley, 1977 Inhassoro – IICT/R 3/1955; Gorongosa – IICT/R 48/1963, 84–86/1955, 160/1955; Nhaluiro – IICT/R 2/1971; Nova Mambone – IICT/R 12/1956 Manaças (1959) 9
Psammophylax Fitzinger, 1843 Psammophylax tritaeniatus (Günther, 1868) Metengo Balama – IICT/R 3/1949; Mozambique [undetermined locality] – IICT/R 252 Manaças (1959) 2
Xenocalamus Günther, 1868 Xenocalamus bicolor lineatus Roux, 1907 Manhiça – IICT/R 1589/1948, 1688/1948 Manaças (1952, 1959) 2
Elapidae Boie, 1827 Aspidelaps Fitzinger, 1843 Aspidelaps scutatus Smith, 1849 Mauele – IICT/R 1174/1948 Manaças (1961) 3
Dendroaspis Schlegel, 1848 Dendroaspis angusticeps (Smith, 1849) São Martinho do Bilene – IICT/R 70A/1955 1
Dendroaspis polylepis Günther, 1864 Maputo – IICT/R 173/1955; Mozambique [undetermined locality] – IICT/R 88/1948 2
Elapsoidea Bocage, 1866 Elapsoidea boulengeri Boettger, 1895 Guijá – IICT/R 969-1948; Mauele – IICT/R 1193/1948, 1218/1948 2
Elapsoidea sundevallii Smith, 1848 Mauele – IICT/R 1218/1948 1
Naja Laurenti, 1768 Naja annulifera Peters, 1854 Moamba – IICT/R 1278/1948; Mozambique [undetermined locality] – IICT/R Moz9 2
Naja mossambica Peters, 1854 Chinde – IICT/R 4/1949; Gorongosa – IICT/R 38/1955 Manaças (1959) 2
Colubridae Oppel, 1811 Crotaphopeltis Fitzinger, 1843 Crotaphoepltis hotamboeia (Laurenti, 1768) Mauele – IICT/R 1276/1948; Nova Mambone – IICT/R 3/1956; Porto Henrique – IICT/R 1641/1948; São Martinho do Bilene – IICT/R 170/1955; Gorongosa – IICT/R 46/1955, 53/1955, 55/1955, 81/1955, 105/1955, 119/1955; Mozambique [undetermined locality] – IICT/R Moz18 Manaças (1959) 11
Dasypeltis Wagler, 1830 Dasypeltis scabra (Linnaeus, 1758) Maqueze, Alto Changane – IICT/R 1501/1948 Manaças (1959) 1
Dipsadoboa Günther, 1858 Dipsadoboa aulica (Günther, 1864) Chibuto – IICT/R 1324/1948; Nova Mambone – IICT/R 1A/1956 Manaças (1959) 2
Dispholidus Duvernoy, 1832 Dispholidus typus (Smith, 1828) Chibuto – IICT/R 1422–1423/1948; Gorongosa – IICT/R 45/1955, 107/1955, 118/1955, 136/1955; Guenguela stream, Chibuto – IICT/R 1424/1948; Manhiça – IICT/R 1683/1948; Nova Mambone – IICT/R 2A/1948; São Martinho do Bilene – IICT/R 171/1955; Manaças (1959) 10
Philothamnus Smith, 1840 Philothamnus angolensis Bocage, 1882 Angónia – IICT/R 5/1949; Gorongosa – IICT/R 45A/1955, 58/1955, 130/1955, 154/1955, 157–158/1955; Mozambique [undetermined locality] – IICT/R Moz20 8
Philothamnus hoplogaster (Günther, 1863) Chizizira – IICT/R 15/1964; Gorongosa – IICT/R 123/1956, 153/1956, 155/1956, 159/1956; Nova Mambone – IICT/R 1/1956 Manaças (1959) 6
Philothamnus semivariegatus (Smith, 1840) Chizizira – IICT/R 11/1964; Gorongosa – IICT/R 103/1955; Meponda, Niassa lake – IICT/R Moz2; Meponda – IICT/R Moz4; Nova Mambone – IICT/R 4/1956; Sussundenga – IICT/R 14/1964; Mozambique [undetermined locality] – IICT/R A/1948, Moz24 Manaças (1959) 8
Telescopus Wagler, 1830 Telescopus semiannulatus Smith, 1849 Nova Mambone – IICT/R 8/1956; Pafuri – IICT/R 725/1948 2
Thelotornis Smith, 1849 Thelotornis capensis Smith, 1849 Gorongosa – IICT/R 50–51/1955, 130A/1955, 151–152/1955 5
Natricidae Bomaparte, 1838 Natriciteres Loveridge, 1953 Natriciteres olivacea (Peters, 1854) Gorongosa – IICT/R 52/1955, 120/1955; Tchizine – IICT/R 12/1964 Manaças (1959) 3
TOTAL NUMBER OF REPTILES SPECIMENS 649

Geographically, these specimens are from 62 different localities distributed in the nine provinces of the country (Table 10; Fig. 13). The temporal range of collecting events is from 1947 to 1971, although the majority of the specimens were collected in 1948 and 1955. The main contributors to the collections were Fernando Frade and his team during the Scientific Mission to Mozambique, and Marques da Silva (dates of birth and death unknown) from the Trypanosomiasis Eradication Mission. Several specimens were also collected by staff of the Zootechnical Post in Angonia.

Gazetteer of Mozambique localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Province Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Gaza Alferes Chamusca, canal de rega (Guijá) [or Viveiro de B.T.L., Guijá, Alferes Chamusca] Caniçado [or Caniçado, watering channel; or Caniçado, B.T.L. vivarium] -24.49750, 33.01333 9059 34 7/38
Gaza Barragem do Rio Limpopo Limpopo river Dam -24.384942, 32.862182 3182 36 1/2
Gaza Chibuto Chibuto -24.689738, 33.538492 3660 104 5/9
Gaza Chicuala-Cuala Chicualacuala [a.k.a Eduardo Mondlane] -22.079167, 31.678611 3036 456 1/1
Gaza Guijá Guijá -24.418921, 32.901765 11027 36 5/13
Gaza Ribeira de Guenguela, Chibuto Guenguela stream, Chibuto -24.69473, 33.520468 3113 22 7/79
Gaza Maquese Maqueze -24.279167, 33.566667 3036 23 2/29
Gaza Maquese (Alto Changane) Maqueze, Alto Changane -23.394722, 33.831389 3996 57 3/15
Gaza Margens do Rio Limpopo Limpopo river margins -25.19361, 33.52583 3036 8 2/8
Gaza Pafuri [or Mabaça (Pafuri)] Pafuri -22.453056, 31.332778 500 223 6/7
Inhambane Govuro Govuro -21.33047, 34.59716 3036 127 1/1
Inhambane Homoíne Homoíne -23.884444, 35.151389 3036 108 1/1
Inhambane Inharime Inharrime -24.476944, 35.030278 3036 39 1/1
Inhambane Inhassoro Inhassoro -21.534722, 35.202222 3036 42 1/1
Inhambane Magaiza (Panda) Magaiza, Panda -23.847222, 34.199444 2639 108 2/4
Inhambane Mauele Mauele [undetermined locality] – – – 20/149
Inhambane Mambone Nova Mambone -20.988056, 35.022222 500 5 10/10
Inhambane Panda Panda -24.063333, 34.730278 7220 160 1/1
Inhambane Inhassoro, Vilanculos road between Inhassoro and Vilanculos -21.803504, 35.117303 27302 23 1/1
Inhambane S. Martinho de Bilene São Martinho do Bilene / Lagoa São Martinho / Lagoa Chicungue, Bilene -25.281111, 33.253889 10000 21 8/16
Manica 15 mls SE of Vila Manica, P.E.A. 15 mls SE of Vila Manica, P.E.A. -18.933332, 32.877127 1000 700 1/1
Manica Chimoio [or Vila Perry] Bengo, Chimoio -19.110117, 33.462887 4772 715 3/8
Manica Cafumpe Cafumpe -19.101389, 33.570278 765 715 1/1
Manica Chizizira [or Posto Piscicola Chizizira] Chizizira -19.448611, 33.297222 3433 536 6/9
Manica Sussundenga Sussundenga -19.403889, 33.290278 3036 600 8/20
Maputo Boame [or Boane] Boane -26.041667, 32.325278 3036 51 8/24
Maputo Centro Social Centro Social [undetermined locality] – – – 5/6
Maputo Echisa (Maputo) Alto Enchisa -26.329907, 32.264797 4298 47 3/3
Maputo Maluana Maluana -25.495, 32.654167 2000 62 1/1
Maputo Manhiça Manhiça -25.402222, 32.807222 6500 22 19/121
Maputo Lourenço Marques Maputo -25.965278, 32.589167 7000 21 2/2
Maputo Matola Matola -25.962222, 32.458889 7317 42 2/6
Maputo Mohamba [or Mohamba, Chibuto, or Moamba] Moamba -25.596111, 32.243333 3036 112 6/18
Maputo Mucomaze, Maputo Mucomaze -26.733333, 32.816667 3036 30 2/13
Maputo Namaacha Namaacha -25.982624, 32.027959 3036 575 9/40
Maputo Porto Henrique, Maputo [or Porto Henriques] Porto Henrique -26.3, 32.348889 1000 32 5/24
Maputo Ponta do Ouro, Maputo Ponta do Ouro -26.842778, 32.896944 1807 34 1/1
Maputo Umbeluzi Umbeluzi -26.028333, 32.39 1977 16 3/11
Nampula Mutuali Mutuali -14.870556, 37.004444 4000 587 2/2
Niassa Meponda, Lago Niassa Meponda, Lago Niassa -13.421667, 34.871667 2071 476 1/1
Niassa Meponda Meponda -13.421667, 34.871667 3036 483 3/3
Niassa Mitucué Mountains, Niassa Mitucué Mountains -14.731545, 36.66941 5311 1136 1/2
Sofala Dondo Dondo -19.609444, 34.743056 1787 55 3/10
Sofala Margens do Rio Nhandare, Gorongosa [or Vila Paiva de Andrada, Rio Nhantare] Nhandare river margins, Gorongosa [or Nhandare river, Gorongosa] -18.74608, 34.05541 35862 205 2/8
Sofala Serra da Gorongosa Mount Gorongosa -18.41098, 34.086773 13178 1487 3/24
Sofala Vila Paiva de Andrada [or Gorongosa] Gorongosa -18.684092, 34.070301 1815 372 40/331
Tete Angónia Angónia -14.715833, 34.373056 40000 1250 1/1
Tete Fumo- Chide, Angónia Chidê -14.619722, 34.085278 460 1147 1/1
Tete Lifidzi (Angónia) Lifidzi, Angónia -14.55, 34.233333 1800 1250 7/23
Tete Metengo, Balama, Angónia Metengo Balama -14.848056, 34.526111 2000 1370 2/2
Tete Nhaluiro [or Nhaluiro Velho, or Ribeira Nhaluiro] Nhaluiro -15.543889, 31.909722 3036 481 6/16
Tete Vila Coutinho Ulongué -14.574444, 34.306111 3036 1295 3/3
Tete Viola (Mazoe River Bridge), P.E.A. Viola, Mazowe River Bridge -16.53173, 33.428165 3036 173 1/1
Zambézia 14 mls west of Morrumbala 14 mls west of Morrumbala -17.354788, 35.472782 1000 309 1/1
Zambézia Mocuba Mocuba -16.845556, 36.964167 1439 148 1/1
Zambézia Rio Licungo Licungo river [undetermined locality] – – – 1/1
Zambézia Rio Luá (Ile) Luá (Ile) river -15.92, 37.119167 6000 415 1/2
Tchizine Tchizine [undetermined locality] – – – 1/1
Tchizigine Tchizigine [undetermined locality] – – – 1/1
Moçambique Mozambique [undetermined locality] – – – 15/30
Presumably Mozambique – – – 2/2
10.3897/zookeys.1052.64607.figure13 5168176 69759F20-C2BA-5D59-9015-EA76550F44B3

Distribution of the Mozambique localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572781

The collection was primarily studied and published on by Sara Manaças resulting in four publications (Manaças 1950b, 1954, 1959, 1961a), while Clara Ruas published a paper focused on the amphibians (Ruas 2002b). In 1965 the Zimbabwean herpetologist, Donald G. Broadley (1932–2016) described two new species of Platysaurus, the Spotted Flat Lizard, Platysaurus maculatus maculatus (two paratypes) and the Striped Tail Flat Lizard, Platysaurus maculatus lineicauda (one paratype), based on some specimens from this collection (Broadley 1965). The previously unpublished specimens of Wilhelm’s Flat Lizard, Platysaurus intermedius wilhelmi Hewitt, 1909, represent the first records for the subspecies in Mozambique.

Globally, this is the largest collection of amphibians and reptiles from Mozambique with 1168 specimens, followed by those of the TM with 847, the MCZ with 643, and the MNHN with 510 (data retrieved from GBIF.org in February 2021). Other specimens exist in several other museums, with numbers ranging from 132 to 456 specimens per collection (data retrieved from GBIF.org in February 2021).

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018d; https://www.gbif.org/dataset/3c66c8f5-a981-46ea-8b0f-6ae44f799220).

 Macau collection

The most comprehensive account of the herpetofauna of China was provided by Zhao and Adler (1993), listing a total of 661 species. Since then, that number almost doubled and new species are being described every year (Murphy 2016; Kai et al. 2020). However, works dedicated to the herpetofauna of Macau are scarce (e.g., Barros 1978), contrary to those concerning other Chinese territories, such as Hong Kong (e.g., Romer 1975, 1979a, b; Karsen et al. 1998). Easton and Leung (1993) provided the only systematic account of the reptiles and amphibians of Macau that is available in English. This work lists a total of 31 species for Macau and was mostly based on material collected between 1989 and 1992 that was deposited in the BPBM. Zhao and Leung (1999) published an updated checklist (available only in Chinese) of the amphibians and reptiles from Macau and recorded a total of 38 species for the region. The most recently updated checklist of Macau herpetofauna is available on the online database Macau Biodiversity (www.macaubiodiversity.org; accessed 5 February 2021), listing nine species of amphibians and 30 species of reptiles occurring in the territory.

The collection comprises a total of 318 specimens (266 amphibians and 52 reptiles) that include nine species of amphibians and 16 species of reptiles (Table 11). The amphibian collection includes representatives of eight genera and six different families, including two specimens of the iconic South China Giant Salamander, Andrias sligoi (Boulenger, 1924). This collection is dominated by the Asian Toad, Duttaphrynus melanostictus (Schneider, 1799) with 151 specimens, followed by the Banded Bull Frog, Kaloula pulchra Gray, 1831 with 27 specimens, and the Ornamented Pygmy Frog, Microhyla ornata (Duméril & Bibron, 1841) with 18 specimens. Specimens of the Asian Toad account for nearly half of all the material from Macau, while most of the remaining amphibian species are represented by 10 specimens or less. The reptile collection is considerably smaller, with only 52 specimens, but more diverse, covering 13 genera from eight different families. The Gekkonidae is the best represented family among squamates, with 18 specimens from genera Gekko and Hemidactylus, followed by the Agamidae with five specimens of Calotes versicolor (Daudin, 1802) and four specimens of Leiolepis reevesii (Grey, 1831). Most snake species are represented by a single specimen, and Colubridae is the most represented family with seven specimens from four different species.

Overview of the Macau amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
URODELA Duméril, 1806
Cryptobranchidae Fitzinger, 1826 Andrias Tschudi, 1837 Andrias sligoi (Boulenger, 1924) Bought in a market [undetermined locality] – IICT/A A-B/1988 Dias et al. (1994) 2
ANURA Duméril, 1806
Bufonidae (Gray, 1825) Duttaphrynus Frost, Grant, Faivovich, Bain, Haas, Haddad, de Sá, Channing, Wilkinson, Donnellan, Raxworthy, Campbell, Blotto, Moler, Drewes, Nussbaum, Lynch, Green & Wheeler, 2006 Duttaphrynus melanostictus (Schneider, 1799) Cheoc Van – IICT/A 21-22/1989; Hac-Sá barracks road – IICT/A 81-88/1989, 154-161/1989; Old Military Road – IICT/A 89-100/1989; Hac-Sá – 12/1988, 31/1988, 19-20/1089, 37-43/1989, 153/1989, 165/1989, 183-195/1989; former N°1 post of Maritime Delegation – IICT/A 13/1989, 32/1989, 61-62/1989, 65-70/1989, 164/1989; Maritime Delegation road – IICT/A 1-6/1989; On the Military Road to Coloane village – IICT/A 128/1989; Coloane Alto Military Road – IICT/A 140-152/1989; Garden of Montanha Russa [or Montanha Russa Municipal Park] – IICT/A 23-29/1989, 207-208/1989, 210-227/1989, 230-237/1989, 239-246/1989; Ka-Ho – IICT/A 31/1989; Macau [undetermined locality] – IICT/A 248-252/1989; Bought in a market [undetermined locality] – IICT/A 166-169/1989; Mong-Há Municipal Park – IICT/A 59-60/1989, 247/1989; Picnic Park – IICT/A 33-36/1989; Seac-Pai-Van – IICT/A 13/1988, 117-118/1989 Mendes et al. (1994a) 151
Dicroglossidae Anderson, 1871 Hoplobatrachus Peters, 1863 Hoplobatrachus rugulosus (Wiegmann, 1834) Bought in a local market [undetermined locality] – IICT/A 76-77/1989 2
Fejervarya Bolkay, 1915 Fejervarya limnocharis (Gravenhorst, 1829) Hac-Sá barracks road – IICT/A 163/1989; Old Military Road – IICT/A 101-104/1989; On the road from Maritime Delegation to Cheoc Van – IICT/A 9/1989; On the Military Road to Coloane village – IICT/A 125-127/1989; Garden of Montanha Russa [or Montanha Russa Municipal Park] – IICT/A 124/1989 Mendes et al. (1994b) 10
Microhylidae Günther, 1858 Kaloula Gray, 1831 Kaloula pulchra Gray, 1831 Old Military Road – IICT/A 111-116/1989; Hac-Sá – IICT/A 196-205/1989; On the road from Maritime Delegation to Cheoc Van – IICT/A 7-8/1989; On the Military Road to Coloane village – IICT/A 129-130/1989; Garden of Montanha Russa [or Montanha Russa Municipal Park] – IICT/A 119-123/1989; Macau [undetermined locality] – IICT/A 253/1989; Mong-Há Municipal Park – IICT/A 78/1989 Mendes et al. (1994b) 27
Microhyla Tschudi, 1838 Microhyla pulchra (Hallowell, 1861) Hac-Sá – IICT/A 206/1989 Mendes et al. (1994b) 1
Microhyla ornata (Duméril & Bibron, 1841) Old Military Road – IICT/A 105-110/1989; former N°1 post of Maritime Delegation – IICT/A 15-18/1989, 71-75/1989; On the road from Maritime Delegation to Cheoc Van – IICT/A 10/1989; Coloane Alto Military Road – IICT/A 139/1989; Seac-Pai-Van – IICT/A 14/1989 Mendes et al. (1994b) 18
Microhyla sp. On the Military Road to Coloane village – IICT/A 132-138/1989 7
Ranidae Batsch, 1796 Sylvirana Dubois, 1992 Sylvirana guentheri (Boulenger, 1882) Garden of Montanha Russa [or Montanha Russa Municipal Park] – IICT/A 30/1989 Mendes et al. (1994b) 1
Rhacophoridae Hoffman, 1932 Polypedates Tschudi, 1838 Polypedates megacephalus Hallowell, 1861 On the road from Maritime Delegation to Cheoc Van – IICT/A 11-12/1989; On the Military Road to Coloane village – IICT/A 131/1989; former N°1 post of Maritime Delegation – IICT/A 14/1989 Mendes et al. (1994b) 4
Rhacophoridae Hoffman, 1932 Polypedates Tschudi, 1838 Polypedates megacephalus Hallowell, 1861 Ka-Ho – IICT/A Macau1; former N°1 post of Maritime Delegation – IICT/A Macau2; Macau [undetermined locality] – IICI/A Macau3 43
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 266
REPTILES
CHELONIA Brongniart, 1800
Geomydidae Theobald, 1868 Mauremys Ritgen, 1828 Mauremys reevesii (Gray, 1831) Macau [undetermined locality] – IICT/R Macau1-2 3
SQUAMATA Oppel, 1811
Agamidae Gray, 1827 Calotes Cuvier, 1816 Calotes veriscolor (Daudin, 1802) Hac-Sá – IICT/R 10-11/1988, 28/1988; Ka-Ho – IICT/R 23/1989; Mong-Há Inn – IICT/R 3-1988 5
Leiolepis Cuvier, 1829 Leiolepis reevesii (Gray, 1831) Hac-Sá – IICT/R 13-14/1989, 16-17/1989 Pinheiro (1994) 4
Gekkonidae Gray, 1825 Gekko Laurenti, 1768 Gekko chinensis (Gray, 1842) Mong-Há Municipal Park – IICT/R 20-21/1989; former N°1 post of Maritime Delegation – IICT/R 7-9/1989 5
Hemidactylus Gray, 1825 Hemidactylus bowringii (Gray, 1845) Seac-Pai-Van – IICT/R 15-17/1988; Hac-Sá – IICT/R 18-19/1989 5
Hemidactylus brookii Gray, 1845 Mong-Há Inn – IICT/R 4-5/1988; Coloane – IICT/R 8/1988 3
Hemidactylus garnotii Duméril & Bibron, 1836 Seac-Pai-Van – IICT/R 21-22/1988 2
Hemidactylus sp. Mong-Há Inn – IICT/R 22/1989; Coloane – IICT/R 29-30/1988 3
Scincidae Cuvier, 1808 Scincella Mittleman, 1950 Scincella reevesii (Gray, 1838) Mong-Há Inn – IICT/ 1-2-/1988; Hac-Sá Barracks Road – IICT/R 2/1989; former N°1 post of Maritime Delegation – IICT/R 3-4/1989 5
SERPENTES
Colubridae Oppel, 1811 Lycodon Boie, 1827 Lycodon subcinctus Boie, 1827 On the Military Road to Coloane village – IICT/R 12/1989 1
Ptyas Fitzinger, 1843 Ptyas korros (Schlegel, 1837) Hac-Sá – IICT/R 6/1989, 15/1989; Bought in a local market – IICT/R 41/1988 3
Ptyas mucosa (Linnaeus, 1758) Hac-Sá – IICT/R 6/1988 1
Xenochrophis Günther, 1864 Xenochrophis piscator (Schneider, 1799) Maritime Delegation road – IICT/R 10-11/1989 2
Elapidae Boie, 1827 Bungarus Daudin, 1803 Bungarus multicinctus Blyth, 1861 Bought in a market [undetermined locality] – IICT/R 38/1988 1
Ophiophagus Günther, 1864 Ophiophagus hannah (Cantor, 1836) Bought in a market [undetermined locality] – IICT/R 39/1988 1
Viperidae Oppel, 1811 Trimeresurus Lacepede, 1804 Trimeresurus albolabris Gray, 1842 Natural and Agrarian Museum, Seac-Pai-Van [or Museu Natural e Agrário, Seac-Pai-Van]– IICT/R 5/1989 1
Typhlopidae Merrem, 1820 Indotyphlops Hedges, Marion, Lipp, Marin & Vidal, 2014 Indotyphlops braminus (Daudin, 1803) Hac-Sá – IICT/R 1/1989, 19-20/1988; Seac-Pai-Van – IICT/ R 25/1988 5
Bought in a market [undetermined locality] – IICT/R 24/1988, 40/1988 2
TOTAL NUMBER OF REPTILE SPECIMENS 52

Except for one specimen collected in 1984, all the specimens were collected in November 1988 and July 1989 during two expeditions organised and led by Jaime Augusto Travassos Dias (1920–1999; Dias et al. 1994). Geographically, this material comes from 19 different localities in the Macau Peninsula and Coloane Island (Table 12; Fig. 14). Hac-Sá, in Coloane, and Garden of Montanha Russa in Macau are the best represented localities with 52 and 51 records, respectively, while few other localities exceed 20 records. Some material was purchased at local markets and might have been imported from neighbouring regions of China. This is the case for Andrias sligoi and Ophiophagus hannah (Cantor, 1836), two iconic species that are represented in the IICT collection but were not recorded by Easton and Leung (1993) nor Zhao and Leung (1999).

Gazetteer of Macau localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

Island Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Coloane Chok Van Cheoc Van 22.113993, 113.560058 100 51 1/2
Coloane Coloane Coloane 22.126318, 113.56423 400 63 3/5
Coloane Estrada do Quartel de Hac-Sa [or Estrada do Quartel de Ac-Sa] Hac-Sá Barracks road 22.112826, 113.568982 108 40 3/18
Coloane Estrada Militar Velha Old military road 22.122163, 113.554919 200 57 4/28
Coloane Granja (Serviços Agrários da Câmara Municipal das Ilhas) Natural and Agrarian Museum, Seac-Pai-Van [or Museu Natural e Agrário, Seac-Pai-Van] 22.125826, 113.556575 62 8 1/1
Coloane Hac-Sa [or Ac-Sa] Hac-Sá 22.121577, 113.569413 786 22 9/52
Coloane Posto n°1 former N°1 post of Maritime Delegation 22.113377, 113.550274 60 9 5/26
Coloane Estrada da Delegação [or Estrada da Delegação Marítima] Maritime Delegation road 22.113943, 113.550113 200 7 2/8
Coloane Estrada da Delegação Marítima a Chok-Van On the road from Maritime Delegation to Cheoc Van 22.111574, 113.55314 300 52 4/6
Coloane Estrada Militar a Coloane On the Military road to Coloane village 22.122987, 113.558851 496 92 6/15
Coloane Estrada Militar do Alto de Coloane Alto de Coloane, military road 22.122987, 113.558851 496 92 2/14
Coloane Ka-Ho Ka-Ho 22.133762, 113.576933 26 56 3/3
Coloane Parque de Merendas Picnic Park 22.114778, 113.562985 60 85 1/4
Coloane Siac-Pai-Van Seac-Pai-Van 22.129308, 113.562903 300 13 5/10
Macau Montanha Russa [or Montanha Russa (jardim)] Garden of Montanha Russa [or Montanha Russa Municipal Park] 22.204354, 113.552576 60 22 4/51
Macau Mong-Há Mong-Há Municipal Park 22.20797, 113.548063 225 33 3/6
Macau Pousada Mong-Há Mong-Há Inn 22.206509, 113.548921 40 28 4/6
– Comprada num mercado em Macau [or Comprada em Macau, or Mercado] Bought in a local market [undetermined locality] – – – 6/9
– Macau Macau [undetermined locality] – – – 4/8
10.3897/zookeys.1052.64607.figure14 5168178 4AD761F3-E50E-5758-B5E3-0136EC4899DF

Distribution of the Macau localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572782

This collection was partly studied by Dias et al. (1994), Mendes et al. (1994a, b), and Pinheiro (1994), but none of these authors provided a complete overview of the collection.

Even though specimens from mainland China are common in museum collections, specimens of amphibians and reptiles from Macau are scarce in western museum collections. IICT holds the largest known herpetological collection from that region, with 323 specimens, followed by the BPBM with 27 specimens (Easton and Leung 1993).

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018e; https://www.gbif.org/dataset/7df6557f-1996-4874-a08a-1ac5718ef413).

 Portuguese India (Goa) Collection

The Indian herpetofauna is still poorly known and lacks an updated systematic account. Despite recent efforts to summarise the knowledge of Indian amphibians and reptiles in checklists and field guides (e.g., Daniel 2002; Venugopal 2010; Das and Das 2017), the works of Boulenger (1890), Smith (1931, 1935, 1943), and Sharma (1998, 2007) remain the most comprehensive accounts of India’s herpetofauna. There are approximately 610 species of reptiles (Khandekar et al. 2021) and 472 species of amphibians (Gosavi et al. 2021) currently known from India, of which nearly half are endemic. However, these numbers are expected to increase as several new species have been described in recent years (e.g., Biju et al. 2011; Agarwal et al. 2019; Giri et al. 2019). While there are several accounts of the herpetofauna of former British India (e.g., Günther 1864; Boulenger 1890; Smith 1931, 1935, 1943; Constable 1949), reports on the reptiles and amphibians of former Portuguese territories in India are scarce (see Das 2004 for a detailed history of herpetological research in India). The first contribution from Portuguese naturalists was published by Bocage (1863), reporting only seven species. Later, Ferreira (1897a) provided an account of the Indian reptiles and amphibians present in the collections of the Zoological Section of the National Museum of Lisbon, listing 68 species of reptiles and 8 species of amphibians. All of those specimens were destroyed in a fire in 1978. Themido (1941) gave an account of the small collection of reptiles from Portuguese India present in the Museu Zoológico da Universidade de Coimbra (currently MCUC), covering a total of 12 species of reptiles. After the annexation of Goa by the Republic of India in 1961, the ZSI collected 413 specimens of reptiles from 40 localities in the state of Goa between 1966 and 1969. This material was later examined and published by Sharma (1976), who listed 46 species, including two newly described species of lizards (Cnemaspis goaensis and Lygosoma goaensis).

The collection comprises only 26 specimens (12 amphibians and 14 reptiles) belonging to three different species (Table 13). The Dicroglossidae is the only amphibian family represented in the collection, with 12 specimens of Hoplobatrachus tigerinus (Daudin, 1802). Squamates are represented by the families Agamidae, with 13 specimens of Calotes versicolor, and Scincidae, with only one specimen of Lygosoma punctata (Gmelin, 1799).

Overview of the former Portuguese India amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
AMPHIBIANS
Dicroglossidae Anderson, 1871 Hoplobatrachus Peters, 1863 Hoplobatrachus tigerinus (Daudin, 1802) Santa Cruz [or Calaphur] – IICT/A 15-21/1959; Taleigão – IICT/A 10-14/1959 Manaças (1961) 12
TOTAL NUMBER OF AMPHIBIAN SPECIMENS 12
REPTILES
SQUAMATA Oppel, 1811
Agamidae Gray, 1827 Calotes Cuvier, 1816 Calotes veriscolor (Daudin, 1802) Santa Cruz [or Calaphur] – IICT/R 1-9/1959; Pernem – IICT/R India1-4 Manaças (1961) 13
Scincidae Cuvier, 1808 Lygosoma Hardwicke & Gray, 1827 Lygosoma punctata (Gmelin, 1799) Pernem – IICt/R India5 1
TOTAL NUMBER OF REPTILE SPECIMENS 14

Geographically, this collection covers only three localities in the state of Goa: Santa Cruz (or Calaphur) with 16 records, Taleigão, and Pernem, with five records each (Table 14; Fig. 15). This material was opportunistically collected in November 1959 by Armando Castel-Branco (1909–1977), a researcher for the CZL, while conducting entomological studies in Goa. This modest collection was studied and published by Manaças (1961b).

Gazetteer of the former Portuguese India localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

State Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Goa Pernem Pernem 15.71674, 73.796996 1833 23 2/5
Goa Santa Cruz Santa Cruz [or Calaphur] 15.470833, 73.843056 1833 12 2/16
Goa Taleigão Taleigão 15.4675, 73.821389 1833 16 1/5
10.3897/zookeys.1052.64607.figure15 5168180 D95CB8F6-F737-53F7-B52A-F40EA5346BD6

Distribution of the Goa localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572783

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018e; https://www.gbif.org/dataset/7df6557f-1996-4874-a08a-1ac5718ef413).

 East Timor collection

Despite a series of herpetological surveys that started in the beginning of the twenty-first century (Kaiser et al. 2011; O’Shea et al. 2012), the knowledge of the reptiles and amphibians of this country is still very incipient. The most recent account of the herpetofauna of mainland East Timor was provided by O’Shea et al. (2015) and reports at least 60 species of amphibians and reptiles, including more than 20 undescribed species. A first record of the herpetofauna of Antaúro Island was provided by Kaiser et al. (2013), listing 14 species of reptiles, of which at least five are likely undescribed endemic species. The exclave of Oecusse District, in the western part of Timor Island, was also surveyed in recent years (Sanchez et al. 2012). Historically, even though some authors addressed the herpetofauna of Timor (e.g., van Lidth de Jeude 1895), these efforts are mostly focused on the western part of the island, with records from the territory that is now East Timor being scarce. The Portuguese contribution to the knowledge of the herpetofauna of East Timor started with the explorations of Francisco Newton between 1895 and 1897, whose specimens were examined by Ferreira (1897b, 1898) and were subsequently lost in the fire that destroyed the zoological collections of Museu Bocage, Lisbon, in 1978. Other Portuguese contributions were published by Themido (1941) based on material collected by several contributors and offered to the MCUC. Following Portuguese decolonisation in 1975, Indonesia invaded the country, instituting a period of political instability that halted biological research. This period lasted until 2002, when East Timor regained independence, and was followed by a series of surveys that greatly improved previous knowledge of the country’s herpetofauna and are expected to continue increasing the number of known species (Kaiser et al. 2011, 2013; O’Shea et al. 2012, 2015; Sanchez et al. 2012). Most specimens vouchered during these recent surveys have been deposited in the collections of the USNM.

The material from East Timor constitutes the smallest herpetological sub-collection of the IICT, with only 17 reptile specimens. Despite its small size, this collection covers nine species of reptiles from eight different families (Table 15), corresponding to roughly 15% of the total number of species currently known from East Timor. The Gekkonidae is the best represented family, with three specimens of Hemidactylus frenatus Duméril & Bibron, 1836 and two specimens of Gekko gecko (Linnaeus, 1758). With only four specimens, Cylindrophis boulengeri Roux, 1911, is the best represented species. No amphibian species are represented in the collection.

Overview of the East Timor amphibian and reptile collections of IICT.

Family Genus Species Localities – Accession number References Number of specimens
(* denotes a type specimen)
REPTILES
SQUAMATA Oppel, 1811
Agamidae Gray, 1827 Draco Linnaeus, 1758 Draco timorensis Kuhl, 1820 Dili – IICT/R 6-1956, Timor3 Manaças (1956, 1972) 2
Gekkonidae Gray, 1825 Gekko Laurenti, 1768 Gekko gecko (Linnaeus, 1758) Dili – IICT/R 4/1956, Timor6 Manaças (1956, 1972) 2
Hemidactylus Gray, 1825 Hemidactylus frenatus Duméril & Bibron, 1836 Dili – IICT/R 1-3/1956 Manaças (1972) 3
Varanidae Hardwicke & Gray, 1824 Varanus Merrem, 1820 Varanus timorensis (Gray, 1831) Dili – IICT/R 7/1956 Manaças (1972) 1
SERPENTES
Colubridae Oppel, 1811 Dendrelaphis Boulenger, 1890 Dendrelaphis inornatus timorensis Smith, 1927 Dili – IICT/R Timor2, Timor5 2
Elapidae Boie, 1827 Laticauda Laurenti, 1768 Laticauda colubrina (Schneider, 1799) Dili – IICT/R Timor4 1
Viperidae Oppel, 1811 Trimeresurus Lacepede, 1804 Trimeresurus insularis Kramer, 1977 Dili – IICT/R 8/1956 Manaças (1972) 1
Cylindrophiidae Fitzinger, 1843 Cylindrophis Wagler, 1828 Cylindrophis boulengeri Roux, 1911 Dili – IICT/R 9-10/1956, Timor1, Timor8 4
Typhlopidae Merrem, 1820 Indotyphlops Hedges, Marion, Lipp, Marin & Vidal, 2014 Indotyphlops braminus (Daudin, 1803) Dili – IICT/R Timor7 1
TOTAL NUMBER OF REPTILE SPECIMENS 17

Geographically, all specimens were collected in Dili (Table 16; Fig. 16). While a few specimens were collected in 1953 and sent to the CZL by the Portuguese anthropologist Ruy Cinatti (1915–1986), most of the material was collected and offered by Cunha Porto (birth and death dates unknown) in 1956. This small collection was only studied by Sara Manaças and published on twice (Manaças 1956, 1972).

10.3897/zookeys.1052.64607.figure16 5168182 40FCFA1C-1BB8-5B11-BB84-71D734CF5CD2

Distribution of the East Timor localities represented in the IICT herpetological collections.

https://binary.pensoft.net/fig/572784

Gazetteer of East Timor localities of IICT specimens. Latitude and longitude decimal coordinates are presented in WGS-84 projection.

State Verbatim locality Current locality Latitude and Longitude Uncertainty (meters) Elevation (meters) Number of taxa/records
Dili Dili Dili -8.558611, 125.573611 6710 3 8/14

Herpetological specimens from East Timor are rare in museum collections, with recent surveys contributing relevant collections for MCZ, with 256 specimens, and the NCSM, with 30 specimens (data retrieved from GBIF.org in February 2021). Material from other collections worldwide generally do not exceed 10 specimens (data retrieved from GBIF.org in February 2021). There is also a small collection with 23 specimens at the MCUC (Themido 1941).

The dataset of this collection is available at GBIF (Ceríaco and Marques 2018e; https://www.gbif.org/dataset/7df6557f-1996-4874-a08a-1ac5718ef413).

 Discussion The importance of the <abbrev content-type="institution" xlink:title="Instituto de Investigação Científica Tropical, Lisboa, Portugal" id="ABBRID0EBTNM">IICT</abbrev> herpetological collection for research and conservation

As part of an institution aimed at the scientific study of the Portuguese colonial territories, the CZL and its researchers had privileged access to the fauna of those areas. This resulted in very important herpetological collections, which rank amongst the largest available collections for some of the Portuguese speaking countries in Africa. Within the Portuguese natural history context, the IICT herpetological collections rank amongst the largest of the country.

Besides its overall diversity and extensive geographic distribution (297 species from 258 unique localities in eight countries), the collections house a considerable amount of type material. This type material belongs to nine different taxa, mainly from São Tomé & Príncipe (18 types), Angola (25 types), and Mozambique (three types).

As demonstrated by the role of the IICT specimens in the description of new species (Ceríaco 2015; Ceríaco et al. 2016, 2017, 2020a, 2021a; Soares et al. 2018; Hallermannn et al. 2020), national checklists and atlases (Ceríaco et al. 2018a; Marques et al. 2018), as well as phylogeographic studies (Ceríaco et al. 2020c), the collections housed in the IICT continue to play an important role on the development of herpetology in a global context, especially in the Portuguese speaking world. This is critically important, as this collection, originally created in a colonial context, can now serve to enhance and foster scientific cooperation and knowledge transfer between former colonial powers and independent countries.

Currently, the collection is fully catalogued and accessible. It is regularly consulted by international researchers, Portuguese and foreign students for their Master and PhD theses, and used in conservation assessments (Ceríaco and Marques 2019).

 The future of Portuguese natural history collections

Natural history collections are an irreplaceable resource for the study of past and present biodiversity and its future conservation, as well as an invaluable resource for teaching and training students (Miller et al. 2020). The recent global pandemic has brought attention to how these collections can be used in multidisciplinary studies such as helping to understand emerging diseases (Cook et al. 2020).

Despite their scientific importance and potential use for research, biodiversity conservation, and teaching, natural history collections currently face serious challenges (Kemp 2015). These challenges vary from country to country due to specific economic and political situations and different institutional backgrounds. This is particularly true for southern European countries whose funds for research are not comparable to their northern European and North American counterparts. Recently, Italian researchers pointed out the severe risk of the neglect and loss of the country’s natural history collections, due to severe disinvestment and lack of proper and sustainable management strategies (Andreone et al. 2014; Andreone 2015). Although perhaps not as dramatic as the Italian scenario, the Portuguese situation, for which the IICT collections serve as an example, is far from ideal.

Altogether, the Portuguese natural history collections house more than 3,500,000 specimens. Although this number may seem small when compared to other major natural history collections in Europe, the Portuguese natural history collections are rich in specimens from biodiversity hotspots, such as the Mediterranean region and the tropical regions spanned by their former colonial possessions and range from the mid-eighteenth century to the present day. As recently noted by Monfils et al. (2020), smaller, regional collections play a fundamental role in modern biodiversity research and conservation, comparable to those of larger museums. This is especially true for the IICT collections, which represent, for some African countries, the largest (or among the largest) herpetological collections available.

Despite its national and international importance, the staff allocated to the curation, preservation, and study of this collection is limited. None of the three main museums, MUHNAC, MHNC-UP, and MCUC, have collection managers as part of their permanent staff, and very few collections have permanent curators. The curatorial staff in the three main museums is composed of movable groups of volunteers, including graduate students, post-doctoral researchers, grantees from different research projects, and retired professors or researchers at other national institutions.

The major Portuguese natural history collections are currently part of larger interdisciplinary university museums, which were recently created through the merging of former more discipline-oriented museums. The University of Lisbon manages MUHNAC, which houses zoological, botanical (herbaria), geological, paleontological, anthropological, and scientific instrument collections, as well as an assortment of memorabilia and smaller collections related to the history of science in the university. The IICT collections, despite being institutionally independent from the museum, are in practice managed as part of the museum collections, as they share the same space and staff. Similarly, the University of Porto and the University of Coimbra, respectively, manage MHNC-UP and MCUC, which also house comparably diverse and interdisciplinary collections from the historical museums of both universities. With the exception of the Herbarium of the University of Coimbra (COI; the largest Portuguese herbarium, with ca. 800.000 specimens) which is run by the Department of Life Sciences, these interdisciplinary museums are all directly under the management of their respective dean’s offices. Other collections, usually run by research groups in departments, research centres, or municipalities, also exist, but these are usually smaller in number of specimens and have very limited taxonomic scope.

There are various reasons why these disciplinarily distinct collections were merged within the university museums structure, although one of the major drivers was the economic and management burden caused by having several independent museums within the universities. There are both advantages and disadvantages to such mergers, which have raised several challenges at the methodological, management, and even epistemological levels. Curating a biological collection is radically different from curating a collection of historical scientific instruments and developing a functional database that serves both the interests and needs of curators of almost opposite typologies of collections is challenging.

Being a university museum is, a priori, a very interesting opportunity in favour of natural history collections, as this institutional relation can foster important research collaborations and teaching partnerships between the museum and the rest of the academic community, from professors and researchers to graduate and undergraduate students (Cook et al. 2014). Some of the larger and more important natural history collections in the USA are part of universities, as it is the case of the MCZ, MVZ, YPM, or the natural history museums of the universities of Kansas, Michigan, and Florida, and their collections are used on a daily basis by the university community, as well as by national and international researchers. Contrary to this advantageous relationship between natural history collections and universities, the Portuguese case has produced different outcomes. Coming from decades of abandonment, these collections are generally perceived by the academic community as the dusty remains of the past practices of science, cumbersome to manage and use, and mostly oriented to the low-impact factor science of taxonomy. Merging it with other types of museum material, such as old scientific instruments and academic memorabilia, has contributed to reinforce the idea of museums as repositories of historical heritage, time capsules of the science of the past (Lourenço and Dias 2017), rather than tools of modern and impactful research.

This association is pernicious and has consequences for the relationship between the museum and its academic community. Firstly, it has led to a physical and emotional separation of professors, researchers, and students from the museum. This has resulted in several immediate problems, such as the abandonment of systematic and taxonomic studies associated with the collections, fostering the already worrisome distance between taxonomists and the rest of the academic community and the well-known negative consequences that this has for biodiversity studies as a whole (Britz et al. 2020). The lack of continuity in the use of collections and the transmission of collections-related practices has led to the loss of basic curatorial and natural history competencies by the community, such as specimen collecting, fixation, and taxidermic techniques, natural history collection management, etc. Although specimen collecting remains an essential tool for biological research (Rocha et al. 2014) and enriching collections is still fundamental to keeping natural history collections relevant for future research (Hope et al. 2018), Portuguese natural history collections are experiencing a considerable deceleration in accessioning new specimens, with some natural history collections not having incorporated any newly collected specimens in the last two decades.

Divorced from its research and teaching objectives, the collection staff is usually reduced to a minimum, which has immediate consequences for the curation and maintenance of its collections, including cataloguing and digitising, leading to drastic limitations on accessibility. This lack of accessibility contributes to a taxonomic impediment (Coleman 2015), frustrating the users of these collections and impeding, rather than supporting, research. This situation further confirms the current general idea of the museum as a dusty place, incapable of supporting modern research, creating a vicious circle. Unfortunately, these consequences have far-reaching effects on a global scale. As a considerable percentage of natural history specimens housed in Portuguese natural history collections originated in the former Portuguese colonial territories in South America, Africa and Asia, all of them important biodiversity hotspots but currently suffering from major threats to biodiversity, the lack of research in and accessibility to these collections is a major challenge for international researchers, particularly those in these megadiverse and developing countries (Romeiras et al. 2014; Neves et al. 2018; Ceríaco and Marques 2019) .

Natural history collections in Portuguese universities risk being considered simply as the historical heritage of the universities, becoming displays of past glories, mostly used in commemorative and outreach programs. This historical nature also causes some practical challenges in terms of their use in exhibitions other than those more focused on history. As many of these collections were assembled for research purposes from the mid-eighteenth century to the mid-twentieth century, their aesthetic and pedagogic value for exhibitions is limited. The concerns raised by the natural history collections being considered as historical heritage also causes problems regarding the accessibility and use of collections. As the first author personally experienced, researchers are sometimes blocked from the study of fluid preserved specimens simply because this would mean that the jar containing those specimens would have to be opened and that would destroy its historical sealant.

Recently, Portuguese natural history collections have been included in important international initiatives aimed at promoting their use, such as DiSSCo, a pan-European Research Infrastructure (Addink et al. 2019). This is a critical opportunity to review current practices and strategies. Natural history collections, although an important part of the historical scientific heritage of Portuguese universities, need to be acknowledged for their full potential and scientific importance. In the current biodiversity crisis, natural history collections should not be seen, managed, and funded at the same level as academic memorabilia or historical scientific instruments (notwithstanding their importance), but as fundamental tools for the study of the earth’s biota and its conservation. In order to achieve this, a new generation of museum naturalists needs to be trained and promoted.

Interestingly, the problems surrounding the Portuguese natural history museums are not new but are a recurrent situation. In 1865, the Portuguese herpetologist José Vicente Barbosa du Bocage (1823–1907), then director of the Zoological Section of the National Museum of Lisbon (precursor of current day MUHNAC), wrote a brief report about the state of the museum (Bocage 1865). The following statement, transcribed from Bocage’s (1865) mid-nineteenth century report, still applies:

"In the current conditions, with the present organisation, the Lisbon museum not only is unable to develop and prosper but will shortly precipitate into a fast and lethal decadence. There is no staff to study part of its collections, there is no staff dealing with its conservation, there is no staff to prepare a portion of its specimens, there are no resources that can be used to enrich its collections. What more can I do besides ask for immediate help? There may be people amongst us that consider this establishment useless, and this may even be the most predominant feeling in the country; but in this case, logic demands that it cannot remain in its current conditions, but rather needs to be closed. To have or to not have a zoological museum is the first thing that needs to be decided, but if we decide to have one, the triumph of the status quo can’t be the corollary of the needed actions."

 A protocol to rescue abandoned natural history collections

After decades of being in limbo, world natural history collections are currently in a state of flux. Several major international initiatives, such as iDigBio (www.idigbio.org) in the USA and DiSSCo (http://www.dissco.eu) in Europe, are driving an important and almost revolutionary change on how society sees, uses, and values these resources. This revolution aims to foster the use of natural history collections data through digitisation and global accessibility (Hendrick et al. 2020). Modern technologies allow us to revisit specimens in ways that seemed like science fiction just a few years ago, extending the specimens beyond their traditional use and adding new layers of information (Webster 2018).

Yet not all collections present the minimum standards for joining the ongoing revolution, especially abandoned collections. Due to lack of proper curation, lack of accessibility, inconsistent internal organisation, and poor conservation, these abandoned collections risk being left aside, simply because in their current state they are almost unusable. This is not only a problem for the collections but a loss of critical scientific data. Unfortunately, this situation is not exclusive to the IICT collections; several collections around the world are in a similar or worse state.

Based on our experience with the recovery of the IICT herpetological collection, we have developed a simplified workflow of ten steps (Fig. 17) for dealing with abandoned collections. For the purpose of this protocol, we assume that the legal ownership of the collection is clarified (i.e., that the collection has a legal owner), and it is accessioned in an institution. In the cases for which the legal ownership is not known, this should be addressed first.

10.3897/zookeys.1052.64607.figure17 5168184 F766DC0C-5EA4-5C97-A91E-20601E452993

Ten steps workflow for recovering abandoned collections.

https://binary.pensoft.net/fig/572785

Designation of a recovery team: The recovery team should be constituted by experts on the taxa (taxonomists) represented in the collection, collection managers or individuals trained in natural history curatorial practices, and volunteers or students. The presence of experts on conservation and restoration is desirable but depends on the particular collection. Health inspectors and other appropriate authorities should be called to assess potential dangers and hazards that the collection may pose to public health and security.

Inspection of the collection and specimens: An initial inspection of the collection allows the team to understand the challenge that lies ahead. During this inspection the team should record the number of specimens in the collection, identify the main conservation techniques used (taxidermy, osteological preparation, fluid preservation, etc.) and the main problems affecting the collection (e.g., pests, loss of preservative fluids, environmental problems in the storage area). Estimate how many specimens will need more complete intervention, and which materials, chemicals, and other gear are needed to proceed. The team should gather all available catalogues, logs of databases, field notebooks, and other documentation associated with the collection. The team should evaluate whether the place where the collection is housed has sufficient space and laboratory facilities to safely carry out the recovery procedures.

Revision of the available metadata: It is fundamental that all the available data associated with the collection and specimens be gathered and reviewed. Without this data, specimens will lose their scientific importance. This associated metadata may be in a variety of formats such as catalogues, databases, field notebooks, tags associated with the specimens, labels on containers or specimens, manuscript documentation, or data from studies of the specimens. This metadata should never be dissociated from the specimen, even if the team finds it to be outdated or not entirely correct. All metadata, if possible, should be digitised. Field notebooks and old catalogues can be digitised and converted to pdf format, while old labels can be digitally photographed. Original documentation should be kept and deposited in an appropriate place that is accessible to researchers, curators, and collection managers.

Review of available published sources: Many collections were studied and published on by researchers in the past. These publications often have additional data regarding the collections or specimens that should, whenever possible, be cross-linked with the specimens in the database. Publications related to the collection vary in format and content: they may be books or papers in scientific journals, and can provide more details on the locality, morphology, or nomenclatural type status of the specimen, etc. Specimen tissues may have sequences deposited in databases such as GenBank (www.genbank.org) or specimens may have been CT-Scanned, radiographed, or photographed and have morphological data available in morphology databases such as MorphoSource (www.morphosource.com) or Morphobank (www.morphobank.org).

Taxonomic revision of individual specimens: All specimens should be individually inspected by a trained taxonomist to review the available identification and confirm, correct, or update it as necessary. The identification should take into account the associated metadata and all published sources on the specimen. Identification techniques vary across taxonomic groups, thus the need for a trained taxonomist. New labels should be created and associated with the specimen, but always keep all the original labels.

Repairing specimens and replacing containers: As each specimen is individually examined for taxonomic purposes, the team will be able to evaluate the condition of the specimen and container. Some specimens will require intervention (e.g., rehydration, cleaning, consolidation of taxidermy mounts, etc.), and some will have problems with their containers (broken containers, evaporation problems, etc.), which can either be repaired or replaced with new containers. For fluid preserved specimens see Simmons (2014), for taxidermy see Ramotnik (2006), for entomological collections see Robinson (2008). The Society for Preservation of Natural History Collections (SPNHC; www.spnhc.org) has online resources regarding this topic.

Preparing or identifying a new storage location: In most instances abandoned collections are kept in insalubrious or inadequate repositories. This is one of the major drivers of collection problems. The team should evaluate the current storage area, determine whether the environmental conditions are appropriate for the collection, if cleaning or adaptations are required, or if it is preferable to move the collection to new repository. For a better understanding of the management and conditions for collection storage, see Elkin and Norris (2019).

Cataloguing, digitisation, and georeferencing protocols: If the collection is not catalogued (i.e., if the specimens do not have a unique identifier), each specimen should be assigned an individual catalogue number. All the data gathered and reviewed during this process should be digitised and formatted according to standard formats, such as DarwinCore (http://dwc.tdwg.org), in order to follow the FAIR standards (Wilkinson et al. 2016) and be shared in major international databases, such as GBIF (www.gbif.org). Whenever possible, locality data should be georeferenced using established protocols (see Chapman and Wieczorek 2020).

Make the collection accessible to the public: Whenever the recovery process is finished, the collection should be made accessible to the public, both physically and virtually through the museum databases and the publication of the datasets on GBIF (www.gbif.org). This will ensure that the collection is visible and usable by any interested parties. A usable and useful collection is the best insurance against future abandonment.

Publish: The team should publish the results of the recovery process, including any taxonomic or scientifically relevant findings they make.

Acknowledgements

The authors thank Luis Mendes, António Bívar de Sousa, and Margarida Pinheiro for granting access to the Centro de Zoologia in the early stages of this work, and for sharing important information regarding the collection. Vitor Gens and Branca Moriés provided important bibliographical and archival data. Leonor Brites Soares, Daniel Mameri, Madalena Marques, Eduardo Marques, and Catarina Mouta helped during the transfer of the collections to MUHNAC and throughout the recovery process. Leonor Brites Soares, Daniel Mameri, and Diogo Parrinha were funded by the Portuguese e-infrastructure for information and research on biodiversity (PORBIOTA). Mariana P. Marques is currently funded by Fundação para a Ciência e Tecnologia (SFRH/BD/129924/2017). During part of this project, Luis M. P. Ceríaco was funded by a JRS Biodiversity Foundation Grant awarded to Aaron M. Bauer and David C. Blackburn. Greg Watkins-Colwell (Yale Peabody Museum of Natural History) and Aaron M. Bauer (Villanova University) are thanked for their comments and insights on initial versions of this manuscript. John Simmons, Oleksandr Zinenko and a third anonymous reviewer provided important suggestions to the improvement of the manuscript. This research has benefited from the use of the infrastructure PRISC (Portuguese Research Infrastructure of Scientific Collections.

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