Input file MrBayes CO1 #NEXUS begin mrbayes ; set autoclose=yes nowarn=yes ; log start filename=log.txt exe Synema_globosum_CO1T3.nex ; charset CO1_12 = 1-1239\3 2-1239\3; charset CO1_3 = 3-1239\3; partition full = 2 : CO1_12, CO1_3 ; set partition = full ; lset applyto = (all) nucmodel=4by4 nst=mixed rates=invgamma ; unlink statefreq =(all) revmat =(all) shape =(all) pinvar=(all) prset applyto = (all) ratepr=variable ; mcmc nruns = 4 ngen=20000000 temp=0.1 nchain=4 samplefreq=1000 ; mcmc ; sump burninr=0.5 ; sumt burinfr=0.5; log stop; end; Input file MrBayes ITS2 #NEXUS begin mrbayes ; set autoclose=yes nowarn=yes ; log start filename=log.txt exe Alignment_ITS_Synglob_Cymbacha.nex charset ITS2 = 1-386 partition full = 1 : ITS2; set partition = full ; lset applyto = (all) nucmodel=4by4 nst=mixed rates=invgamma ; unlink statefreq =(all) revmat =(all) shape =(all) pinvar=(all) prset applyto = (all) ratepr=variable ; mcmc nruns = 4 ngen=20000000 temp=0.1 nchain=4 samplefreq=1000 ; mcmc ; sump burninr=0.5 ; sumt burinfr=0.5; log stop; end; Input files RAxML CO1 raxmlHPC -s Synema_globosum_CO1T3.phy -n CO1_RaxML_plus_outgroup -m GTRCAT -p 2135246 -f a -x 1434897 -N 1000 -q partitionfileCO1_plus.txt [PartitionfileCO1_lpus.txt] DNA, CO1_firstSecond = 1-1239\3, 2-1239\3 DNA, CO1_third = 3-1239\3 Input files RAxML ITS2 raxmlHPC -s ITS2_Synglob_Cymbacha.phy -n ITS2_RaxML_plus_outgroup -m GTRCAT -p 2135246 -f a -x 1434897 -N 1000 -q partitionfileITS2.txt