"Gene Primer";"Forward (F) Reverse (R)";"Sequence 5’–3’";Reference;"Annealing °C"
CO1;–;–;–;–
LCO1490;F;GGTCAACAAATCATAAAGATATTGG;"(Folmer et al. 1994)";"50/48 °C"
ChelicerataR2;R;GGATGGCCAAAAAATCAAAATAAATG;"(Barrett and Hebert 2005)";–
"C1-J- 2183";F;CAACATTTATTTTGATTTTTTGG;"(Folmer et al. 1994)";"47 °C"
C1-N-2778;R;GGATAATCAGAATATCGTCGAGG;"(Simon et al. 1994), (Barrett and Hebert 2005)";–
ITS2;–;–;–;–
ITSf;F;"TCCTCCGCT TATTTATATGC";"(Agnarsson 2010)";"50 °C"
ITSr;R;GGGTCGATGAAGAACGCAGC;–;–
Wolbachia;–;–;–;–
wspF;F;TGGTCCAATAAGTGATGAAGAAACTAGCTA;"(Jeyaprakash and Hoy 2000)";"53 °C"
wspR;R;AAAATTAAACGCTACTCCAGCTTCTGCAC;–;–