Phylogenetic tree of Pristimantis spp. based on mtDNA genes 16S and COI performed by a Shimodaira-Hasegawa approximate likelihood ratio test (SH-aLRT test). Numbers on nodes indicate estimated SH-aLRT support/bootstrap support with SH-aLRT values ≥ 80% are considered reliable for a clade (Guindon et al. 2010). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Colored bars and G-numbers represent the groups generated by ABGD analysis (see results of phylogenetic analysis and Suppl. material 1: Table S1 for more details).