Corresponding author: Jianguo Wang (
Academic editor: M. Alonso-Zarazaga
This study describes a new species,
Lai S, Zhang L, Li Y, Wang J (2021) A new species, a new combination, and a new record of
The genus
The catalog of
The
In this study, we describe a new species of
Adults of the new species were collected by log dissection. The samples were immediately preserved in tubes containing 99.9% ethyl alcohol, which were stored at −20 °C for DNA extraction and examination. Specimens were examined using an Olympus SZX160 stereoscopic zoom microscope. Photographs were taken with a KEYENCE VHX-6000 Digital Microscope System. All photos were further adjusted and assembled with Adobe Photoshop CS6. Body length was measured between the anterior margin of the pronotum and the elytral apex (head not included).
Genomic DNA was extracted from the adult head. The total genomic DNA was extracted from each individual using the Ezup Column Animal Genomic DNA Purification Kit (Sangon Biotech Co. Ltd). Amplification of four gene fragments (COI, EF-1α, CAD, 28S) was made by PCR, using primers (Table
Gene fragments targeted for PCR and the primers used. Sequencing primers were identical to those used in PCR.
Gene | Primer name | Annealing | Primer sequence | Reference |
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COI | S1718 | 46 | 5'-GGAGGATTTGGAAATTGATTAGTTCC-3' |
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A2411 | 5'-GCTAATCATCTAAAAACTTTAATTCCWGTWG-3' | |||
28S | S3690 | 55 | 5'-GAGAGTTMAASAGTACGTGAAAC-3' |
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A4394 | 5'-TCGGAAGGAACCAGCTACTA-3' | |||
EF-1α | S149 | 52 | 5'-ATCGAGAAGTTCGAGAAGGAGGCYCARGAAATGGG-3' |
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A1043 | 5'-GTATATCCATTGGAAATTTGACCNGGRTGRTT-3' | |||
CAD | CAD for4 | 50 | 5'-TGGAARGARGTBGARTACGARGTGGTYCG-3' |
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CAD rev1mod | 5'-GCCATYRCYTCBCCYACRCTYTTCAT-3' |
Material used for phylogenetic analyses, including their GenBank accession numbers.
No. | Taxon | Country | CAD | COI | EF-1α | 28S | Reference |
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1 | China: Jiangxi |
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2 | China: Yunnan |
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3 | Papua New Guinea |
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4 | China: Yunnan |
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5 | China: Yunnan |
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6 | Tanzania |
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7 | Madagascar |
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8 | Papua New Guinea |
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9 | Papua New Guinea |
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10 | China: Sichuan | – |
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11 | Australia |
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12 | China: Jiangxi |
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13 | China: Yunnan |
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14 | China: Jiangxi |
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15 | China: Yunnan |
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16 | China: Jiangxi |
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Male of
Female of
Male of
The species is named for Roger A. Beaver to honor his contributions to the study of platypodines and scolytines.
China (Jiangxi, Fujian).
The species is placed in
1 | Male elytral apex truncate, with a circular, shallow, concave, bluntly margined declivity; sutural apex of declivity slightly dehiscent without apical emargination. Female smaller and stouter, 2.60–2.70 mm long, 2.70–2.75 times as long as wide |
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– | Male elytral apex truncate, with a circular, deep, concave, acutely margined declivity, with a broad, almost circular, apical emargination. Female larger and more elongate, 3.00–3.90 mm long, 2.79–3.44 times as long as wide |
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2 | Male striae weakly impressed on disc of elytra (Fig. |
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– | Male striae moderately impressed on disc of elytra (Fig. |
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7 males, 5 females (
Female of
The specimens in the
Thailand (
4 males, 1 female (
Male of
Diagnostic characters separating
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Body size | Male size 4.56–4.80 mm long, 3.20–3.42 times as long as wide; | Male size 3.32–3.40 mm long, 2.78–3.00 times as long as wide; |
Female size 4.8–5.34 mm long, 3.38–3.43 times as long as wide. | Female size 3.44–3.58 mm long, 2.87–2.93 times as long as wide. | |
Frons | Male frons almost flat, with shallower, irregularly placed punctures; circularly concave in median line. | Male frons coarser, with deeper, irregularly placed punctures; linearly concave in median line. |
Female frons almost flat, without concave around median line. | Female frons concave forming a big, circular impression around concave median line. | |
Elytra | Male without lateral emargination at declivity base, semicircular lateral borders with serrated, lateral tubercles. | Male with lateral emargination at declivity base, semicircular lateral borders rounded, without distinct serrated, lateral tubercles. |
Myanmar, Thailand, Laos (
The species is recorded from trees in the families
Female of
The phylogenetic tree for analyzing the evolutionary relationships of 13 taxa including the ingroups (
Tree topology resulting from Bayesian analysis of four genes. Posterior probabilities are given on the nodes. New species and new combination in boldface.
The genus
We thank Roger Beaver for reviewing earlier manuscript drafts and kindly commenting on the status of the new species. We also appreciate the help provided by Jiaxin Liao and Yufeng Cao in our fieldwork. This research was funded by grant CARS-33-BC2-JX01 from Survey of Bark Beetles on the Rubber Tree in China Project and the National Natural Science Foundation of China (no. 31160380, 31360457, 31760543).