Ancestral reconstruction for geographic basin (Pacific vs. Amazon) in the Pristimantis lacrimosus species group. Reconstructions were based on maximum likelihood inference. Pie charts at nodes represent conditional probabilities for the Pacific (black) and Amazon (green) basins. The asterisk indicates significant support for the Pacific basin as ancestral area for the most recent common ancestor of the P. lacrimosus species group. White circles indicate equivocal state, striped circles indicate distribution outside the Pacific or Amazonian basins (coded as missing data).