Corresponding author: Andrzej Falniowski (
Academic editor: Eike Neubert
A new species of
Hofman S, Rysiewska A, Osikowski A, Falniowski A (2020) A new species of
Mud snails
In September of 2019, in the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River, we found
The snails were collected at the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River (
Localities of
Type locality of
The shells were photographed with a Canon EOS 50D digital camera, under a Nikon SMZ18 microscope. The dissections were done under a Nikon SMZ18 microscope with dark field, equipped with Nikon DS-5 digital camera, whose captured images were used to draw anatomical structures with a graphic tablet. Measurements of the shell (Fig.
Measurements of the shell.
Snails for molecular analysis were fixed in 80% ethanol, changed twice, and later stored in 80% ethanol. DNA was extracted from whole specimens; tissues were hydrated in
DNA coding for mitochondrial cytochrome oxidase subunit I (
Taxa used for phylogenetic analyses with their GenBank accession numbers and references.
Species | References | |
---|---|---|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
|
||
|
||
|
||
|
||
|
||
|
|
|
|
|
|
|
|
|
|
||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Ethanol-fixed specimen (Fig.
Holotype of
Twelve paratypes, ethanol-fixed, in the collection of the Department of Malacology of Jagiellonian University.
Shell minute, nearly planispiral, distinguished from
Shell measurements (in mm) of
A | B | C | D | E | F | G | H | |
---|---|---|---|---|---|---|---|---|
holotype | 0.77 | 1.39 | 0.87 | 0.62 | 0.60 | 1.09 | 0.41 | 0.97 |
2D44 | 0.72 | 1.12 | 0.73 | 0.54 | 0.55 | 0.93 | 0.37 | 0.75 |
2F59 | 0.73 | 1.26 | 0.82 | 0.54 | 0.55 | 0.95 | 0.41 | 0.80 |
2F62 | 0.72 | 1.35 | 0.86 | 0.57 | 0.57 | 1.03 | 0.36 | 0.73 |
2F70 | 0.67 | 1.12 | 0.72 | 0.52 | 0.48 | 0.97 | 0.40 | 0.72 |
2F71 | 0.75 | 1.37 | 0.85 | 0.46 | 0.60 | 1.02 | 0.41 | 0.84 |
|
0.73 | 1.27 | 0.81 | 0.54 | 0.56 | 1.00 | 0.39 | 0.80 |
SD | 0.034 | 0.123 | 0.067 | 0.053 | 0.044 | 0.059 | 0.023 | 0.094 |
Min | 0.67 | 1.12 | 0.72 | 0.46 | 0.48 | 0.93 | 0.36 | 0.72 |
Max | 0.77 | 1.39 | 0.87 | 0.62 | 0.60 | 1.09 | 0.41 | 0.97 |
Shell variability of
Renal and pallial section of the female reproductive organs of
Penis of
The specific epithet
Known from the type locality only.
We obtained six new sequences of
Maximum Likelihood tree inferred from mitochondrial
The sequences of the
p-distances between
mOTU – A | mOTU – B | mOTU – C | mOTU – D | mOTU – E | |
---|---|---|---|---|---|
mOTU – A | – | 0.010 | 0.007 | 0.010 | 0.023 |
mOTU – B | 0.135 | – | 0.017 | 0.020 | 0.033 |
mOTU – C | 0.126 | 0.124 | – | 0.010 | 0.023 |
mOTU – D | 0.146 | 0.138 | 0.124 | – | 0.013 |
mOTU – E | 0.123 | 0.110 | 0.095 | 0.093 | – |
Following the terminology of
Finally, it has to be pointed out that
The study was supported by a grant from the National Science Centre 2017/25/B/NZ8/01372 to AF.