2urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7Eurn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727AZooKeysZK1313-29891313-2970Pensoft Publishers10.3897/zookeys.937.4979449794Research ArticleDrosophilidaeTaxonomyChinaThe genus Scaptodrosophila Duda (Diptera, Drosophilidae), part III: the riverata species group from China, with morphological and molecular evidence for five new speciesLinYong1ChenHong-Weihongweic@scau.edu.cn1Department of Entomology, South China Agricultural University, Tianhe, Guangzhou, 510642, ChinaSouth China Agricultural UniversityGuangzhouChina
20200106202093713916262955966-DCED-51C6-A867-C93553F66A393772774D-6906-4106-8396-96CF4581D79A38860470201202007042020Yong Lin, Hong-Wei ChenThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://zoobank.org/3772774D-6906-4106-8396-96CF4581D79A
A new species group, the riverata species group, is established within the genus Scaptodrosophila based on morphological and molecular evidence for five known and five new species from China: S.abdentatasp. nov., S.cederholmi (Okada, 1988), S.crocata (Bock, 1976), S.paraclubata (Sundaran & Gupta, 1991), S.platyrhinasp. nov., S.puncticeps (Okada, 1956), S.riverata (Singh & Gupta, 1977), S.serrateifoliaceasp. nov., S.sinuatasp. nov. and S.tanyrhinasp. nov. A key to this group is provided. Furthermore, 51 mtDNA COI sequences belonging to S.puncticeps, S.riverata and the five new species are used for verifying species boundaries defined by the morphological data.
cryptic speciesDNA barcodingintegrative taxonomymolecular researchnew species groupDr. Xiaolin Chen (xlchen@ioz.ac.cn: Institute of Zoology, Chinese Academy of Sciences, China)
Dr. Jan Máca (janxmaca@seznam.cz: Czech Agency of Nature Conservation)
Prof. Li Shi (lirui2003@imau.edu.cn: Inner Mongolia Agricultural University, China)
Dr. Domenico Otrantol (domenico.otranto@uniba.it: Department of Veterinary Public Health, University of Bari, Italy)Citation
Lin Y, Chen H-W (2020) The genus Scaptodrosophila Duda (Diptera, Drosophilidae), part III: the riverata species group from China, with morphological and molecular evidence for five new species. ZooKeys 937: 139–162. https://doi.org/10.3897/zookeys.937.49794
Introduction
A total of 12 species groups have been erected within the genus Scaptodrosophila (Duda 1923): albifrontata group (Wheeler and Takada 1966); aterrima group (Tsacas et al. 1988); barkeri group (Bock and Parsons 1978); brunnea group (Tsacas and Chassagnard 1976; Liu et al. 2017); brunneipennis group (Bock and Parsons 1978); bryani group (Throckmorton 1962); coracina group (Mather 1955; Liu and Chen 2018); inornata group (Parsons and Bock 1978); latifasciaeformis group (Burla 1954); rufifrons group (Papp et al. 1999); saba group (Burla 1954) and victoria group (Wheeler 1949). Together, these taxa include ca 300 species. Here we describe five new species from China that are morphologically similar to five known, yet unplaced, species: S.cederholmi (Okada, 1988) from Sri Lanka; S.crocata (Bock, 1976) from Australia; S.paraclubata (Sundaran & Gupta, 1991) from India; S.puncticeps (Okada, 1956) from China, Kuril Islands, Korea, Japan; and S.riverata (Singh & Gupta, 1977) from China, India, Myanmar. These ten species all have a yellowish-brown body; an arista with two dorsal and one ventral branch in addition to a terminal bifurcation; a large facial carina; and developed prescutellar setae. This morphological group is also supported by molecular data for the five new species and two of the five previously described taxa. The combined morphological and molecular evidence supports the establishment of a new species group, the riverata species group, based on five known and five new species from China.
DNA barcoding technology was employed to investigate the relationship of the riverata species group. Based on the results of the phylogenetic reconstruction, 51 barcode sequences of the COI (mitochondrial cytochrome c oxidase subunit I) gene belonging to two known and five new species were used to evaluate these species boundaries.
Materials and methodsSpecimens
The riverata group species were collected by net sweeping from tussocks and tree trunks. All the examined specimens were preserved in 75% ethanol.
Species identification
Total DNA was extracted from the abdominal tissue of samples after the dissection of the genitalia, using the TIANGEN™ DNA extraction kit following the recommended protocol. The COI fragments were amplified using the cycle protocol as in Zhao et al. (2009). The PCR sequencing primer pair was 5′–CGCCTAAACTTCAGCCACTT–3′ (Wang et al. 2006) and 5′–TAAACTTCAGGGTGACCAAAAAATCA–3′ (Folmer et al. 1994). All sequences generated in this study were supplied with BOLD Process ID and GenBank accession numbers (Table 1).
Specimens of brunnea species used for molecular study.
BOLD Process ID
GenBank accession number
Collection sites
S.puncticeps –1
BDORM010–14
KJ841771
Shennongjia, Hubei
S.puncticeps –2
BDORM011–14
KJ841770
Danba, Ganzizhou, Sichuan
S.puncticeps –3
BDORM012–14
KJ841766
Tianmushan, Linan, Zhejiang
S.puncticeps –4
BDORM013–14
KJ841769
Daozhen, Zunyi, Guizhou
S.puncticeps –5
BDORM014–14
KJ841768
Ailaoshan, Jingdong, Yunnan
S.puncticeps –7
BDORM004–14
KJ841761
Shennongjia, Hubei
S.puncticeps –8
BDORM005–14
KJ841762
Miyaluo, Abazhou, Sichuan
S.riverata –1
BDORM008–14
KJ841773
Banli, Chongzuo, Guangxi
S.riverata –2
BDORM009–14
KJ841772
Ailaoshan, Jingdong, Yunnan
S.riverata –3
SDLY001–19
MK335597
Likan, Ximeng, Yunnan
S.riverata –4
SDLY002–19
MK335598
Likan, Ximeng, Yunnan
S.riverata –5
SDLY003–19
MK335599
Likan, Ximeng, Yunnan
S.riverata –6
SDLY004–19
MK335600
Huanglianshan, Lvchun, Yunnan
S.riverata –7
SDLY005–19
MK335601
Huanglianshan, Lvchun, Yunnan
S.abdentata sp. nov. –1
BDORM019–14
KJ841758
Nanling, Shaoguan, Guangdong
S.abdentata sp. nov. –2
BDORM020–14
KJ841757
Muotuo, Lingzhi, Xizang
S.abdentata sp. nov.–3
BDORM021–14
KJ841755
Wangtianshu, Mengla, Yunnan
S.abdentata sp. nov. –4
SDLY006–19
MK335586
Muyiji, Ximeng, Yunnan
S.abdentata sp. nov. –5
BDORM023–14
KJ841759
Wuzhishan, Ledong, Hainan
S.abdentata sp. nov. –6
BDORM024–14
KJ841756
Muotuo, Lingzhi, Xizang
S.abdentata sp. nov. –7
SDLY007–19
MK335587
Muyiji, Ximeng, Yunnan
S.abdentata sp. nov. –8
SDLY008–19
MK335588
Mengdong, Cangyuan, Yunnan
S.abdentata sp. nov. –9
SDLY009–19
MK335589
Mengdong, Cangyuan, Yunnan
S.platyrhina sp. nov. –1
BDORM016–14
KJ841765
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –2
BDORM017–14
KJ841764
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –3
BDORM018–14
KJ841763
Jiangcheng, Simao, Yunnan
S.platyrhina sp. nov. –4
SDLY010–19
MK335590
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –5
SDLY011–19
MK335591
Guanleigang, Mengla, Yunnan
S.platyrhinasp. nov. –6
SDLY012–19
MK335592
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –7
SDLY013–19
MK335593
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –8
SDLY014–19
MK335594
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –9
SDLY015–19
MK335595
Menglun, Mengla, Yunnan
S.platyrhina sp. nov. –10
SDLY016–19
MK335596
Menglun, Mengla, Yunnan
S.serrateifoliacea sp. nov. –1
BDORM006–14
KJ841775
Hesong, Menghai, Yunnan
S.serrateifoliacea sp. nov. –2
BDORM007–14
KJ841774
Hesong, Menghai, Yunnan
S.serrateifoliacea sp. nov. –3
SDLY017–19
MK335602
Mengdong, Cangyuan, Yunnan
S.sinuata sp.nov. –1
SDLY024–19
MK335603
Dayangcha, Kuangdian, Liaoning
S.sinuata sp.nov. –2
SDLY025–19
MK335604
Dayangcha, Kuangdian, Liaoning
S.sinuata sp.nov. –3
SDLY026–19
MK335605
Laobiangou, Benxi, liaoning
S.sinuata sp.nov. –4
SDLY027–19
MK335606
Laobiangou, Benxi, liaoning
S.sinuata sp.nov. –5
SDLY028–19
MK335607
Laobiangou, Benxi, liaoning
S.sinuata sp.nov. –6
SDLY029–19
MK335608
Guojiapuzi, Kuangdian, Liaoning
S.sinuata sp.nov. –7
SDLY030–19
MK335609
Guojiapuzi, Kuangdian, Liaoning
S.sinuata sp.nov. –8
SDLY031–19
MK335610
Guojiapuzi, Kuangdian, Liaoning
S.tanyrhina sp. nov. –1
SDLY018–19
MK335611
Menglun, Mengla, Yunnan
S.tanyrhina sp. nov. –2
SDLY019–19
MK335612
Menglun, Mengla, Yunnan
S.tanyrhina sp. nov. –3
SDLY020–19
MK335613
Wangtianshu, Mengla, Yunnan
S.tanyrhina sp. nov. –4
SDLY021–19
MK335614
Wangtianshu, Mengla, Yunnan
S.tanyrhina sp. nov. –5
SDLY022–19
MK335615
Guanleigang, Mengla, Yunnan
S.tanyrhina sp. nov. –6
SDLY023–19
MK335616
Wangtianshu, Mengla, Yunnan
A total of 51 COI sequences of the riverata group were examined and aligned with MEGA 7.0 (Kumar et al. 2016). Intra- and interspecific genetic distances were calculated for species of the riverata group using the p–distance model (Nei and Kumar 2000). We also conducted character-based species barcoding where fixed sites of one species differed from those of the others; these were manually selected as diagnostic sites (i.e., “pure” diagnostics, Sarkar et al. 2002; Desalle et al. 2005). Three known species: S.melanogaster (GenBank accession number: KR070823), S.rhina (KR070845) and S.scutellimargo (KR070847), were used as outgroup taxa in the phylogenetic analyses. The alignment was subsequently employed to reconstruct a phylogenetic tree using the Neighbor-joining (NJ) method with p-distance model implemented in MEGA 7.0.26 (Kumar et al. 2016). Nodal support values (bootstrap percentages, BPs) were inferred by bootstrapping with 1000 replicates and other default settings.
Description of species
An Mshot Camera was used to photomicrograph all the examined species. All photographs, illustrations, and line drawings were processed with the software Adobe Photoshop 7.0 and Easy PaintTool SAI Ver.1.0.0. The morphological terminology follows McAlpine (1981) and the definitions of measurements, indices, and abbreviations follow Chen & Toda (2001).
The type specimens were deposited in Department of Entomology, South China Agricultural University, Guangzhou, China (SCAU).
Results
The alignment of the 51 COI sequences spans 632 nucleotide sites, with 131 variable sites, 122 of which were parsimony informative. Intra- and interspecific p-distances were provided in Table 2. The results show that the largest intraspecific p-distances within the riverata species group was 0.032 detected in S.puncticeps, followed by 0.016 in S.platyrhina sp. nov. while the minimum interspecific variation was 0.014 detected between S.abdentata sp. nov. and S.tanyrhina sp. nov.
The NJ (Fig. 1) tree shows that this new group is monophyletic with respect to the outgroups. Figure 2 shows nucleotides representing “pure” diagnostic sites for all species of the riverata group; at least one diagnostic site was recognized for each species. For example, site 21 is diagnostic for S.serrateifoliacea sp. nov. with a fixed status of C (Cytosine), rather than T (Thymidine) in the other species.
Diagnostic nucleotide sites in the alignment of COI sequences of the riverata species group. Numbers at the top show the positions of the sites in the COI alignment (584–687 bp in length). Shaded sites are diagnostic for each species. Dashes (–) indicate missing data.
https://binary.pensoft.net/fig/416837
Summary of intra- and interspecific genetic distances.
Species
N
intraspecific genetic distances
interspecific genetic distances
Min. /Max. /Mean ± SD
Min. /Max. /Mean ± SD
S.puncticeps
8
0.000/ 0.032/0.020 ± 0.008
0.071/0.127/0.107 ± 0.017
S.riverata
7
0.000/ 0.006/0.002 ± 0.002
0.049/0.138/0.084 ± 0.032
S.abdentata
9
0.000/ 0.006/0.003 ± 0.002
0.014/0.136/0.073 ± 0.048
S.platyrhina
10
0.000/ 0.016/0.007 ± 0.004
0.022/0.136/0.078 ± 0.046
S.serrateifoliacea
3
0.002/ 0.003/0.002 ± 0.001
0.070/0.138/0.116 ± 0.026
S.sinuata
8
0.000/0.009/0.005 ± 0.003
0.070/0.134/0.113 ± 0.023
S.tanyrhina
6
0.003/ 0.009/0.005 ± 0.002
0.014/0.134/0.070 ± 0.048
N, the numbers of COI sequences involved in distance calculation; Min., minimum; Max., maximum; SD, standard deviation; NA, no applicable.
Body mostly yellow to yellowish brown, without patches; arista with two dorsal branches and one ventral branch in addition to the terminal bifurcation; facial carina large; prescutellar setae usually large, as long as anterior dorsocentral setae; hypandrium usually with a pair of long paramedian setae.
Description.
Male and female: Head (Figs 3, 4, 5A, B, 6A): Eyes red. Ocellar triangle yellowish brown, with 4–6 setae above ocellar setae. Frons about 1/3 width of head, largely yellowish brown to brown, with a few minute setulae medially. Fronto–orbital pale yellowish brown; anterior reclinate orbital seta usually lateral to and about 1/3 length of proclinate orbital seta; posterior reclinate orbital seta larger than other two orbital setae. Pedicel yellowish brown to brown, with a few of fine setae; first flagellomere pale yellowish. Face yellowish brown. Clypeus mostly yellow. Vibrissa prominent; subvibrissal setae small. Gena and postgena narrow, yellowish brown. Palpus yellow to yellowish brown with several setae.
Thorax (Figs 3, 4, 5C–F, 6B, C): Mesonotum yellow to yellowish brown uniformly. Postpronotal lobe yellow with 2–3 long setae, and a few of shorter setae. Acrostichal setulae in ca 8–10 regular rows. Prescutellar setae usually as long as anterior dorsocentral setae. Pleura yellow to yellowish brown uniformly. Katepisternal setae usually subequal. Wing hyaline, sometimes infuscate, lacking patch. Basal medial-cubital crossvein absent. R4+5 nearly parallel distally with M1. Halter pale yellowish. Legs mostly yellow.
Male terminalia (Figs 7–12, 13A–D): Epandrium pubescent, with several setae near posterior margin and ventral corner on each side. Surstylus usually with several peg-like prensisetae on caudal margin, and several setae on outer and inner surface. Hypandrium shallow, usually with a couple of paramedian setae distally. Cercus separated from epandrium, pubescent and setigerous. Paramere developed, with few sensillae. Gonopods (as dorsal extension of the hypandrium, see Ashley and Sinclair 2017) fused with each other, broadened to hood-shaped. Aedeagus bifid, glabrous.
Female terminalia (Figs 7–10, 11E, 13E): Oviscapt mostly yellowish brown to brown, broadened subapically.
In the following descriptions, only those characters differing from the above description were provided.
China: 2♂ (SCAU, Nos 126828, 29), Banli, Chongzuo, Guangxi, alt. 160 m, 21.viii.2004, HW Chen; 1♀ (SCAU, No. 126830), Ailaoshan, Jingdong, Yunnan, 24°32'N, 101°00'E, alt. 2100 m, 21.vi.2009, T Li; 3♂ (SCAU, Nos 110913–15), Likan, Ximeng, Yunnan, 22°39'N, 99°36'E, alt. 844 m, 1.v.2016, YQ Liu; 1♂, 1♀ (SCAU, Nos 110916, 17), Huanglianshan, Lüchun, Yunnan, 23°20'N, 102°23'E, alt. 600 m, 28.x.2018, HW Chen.
Diagnosis.
Surstylus bifid, the upper lobe with about 5 thin, peg-like prensisetae and 1 fine seta, the below one with 1 thin, peg-like prensiseta and 2 fine setae (Fig. 7A, B); gonopods undeveloped (Fig. 7C, D).
Description.
(♂, ♀) Head (Fig. 3A): Frons yellowish brown. Pedicel and first flagellomere maple. Facial carina yellowish, short, 1/3 length as face.
Thorax (Fig. 3C, E): Mesonotum and scutellum yellowish. Acrostichal setulae in ca 8 regular rows. Wings hyaline.
Abdomen (Fig. 3C, E): Tergites and sternites yellowish.
Male terminalia: Epandrium with ca 14 setae near posterior margin and ventral corner on each side (Fig. 7A, B). Hypandrium glabrous (Fig. 7C, D). Paramere slender and curved apically, with 3 sensillae subbasally (Fig. 7C, D).
Female terminalia (Fig. 7E): Oviscapt with 2 long subapical trichoid ovisensilla, 17 peg-like ovisensilla on each side on ventral margins and 5 trichoid ovisensilla on dorsal.
Clypeus reddish brown; palpus pale yellow; gonopods roundly expanded in lateral view. (Fig. 7C, D).
Description.
(♂, ♀) Head (Fig. 3B): Frons reddish brown. Pedicel yellowish brown. Facial carina creamy white, short, 1/3 length as face.
Thorax (Fig. 3D, F): Mesonotum and scutellum yellow. Acrostichal setulae in ca 7 irregular rows. Halter yellowish brown. Wing greyish.
Abdomen (Fig. 3D, F): Tergites yellowish brown.
Male terminalia: Epandrium with ca 21 setae near posterior margin and ventral corner on each side (Fig. 8A, B). Surstylus small with 10 peg-like prensisetae on caudal margin, and ca 16 setae on outer and inner surface (Fig. 8A, B). Hypandrium pubescent near paramedian setae (Fig. 8C, D). Paramere broadened medially with ca 3 sensilla (Fig. 8C, D). Aedeagus broadened apically, curved medially, sickle-shaped anteriorly in lateral view (Fig. 8C, D).
Female terminalia (Fig. 8E): Oviscapt densely covered with peg-like ovisensilla.
China (Zhejiang, Hubei, Hunan, Sichuan, Guizhou, Yunnan), Kuril Islands, Korea, Japan (Kanto).
AnimaliaDipteraDrosophilidae221BABE6-EF36-54F8-8234-2BC846666D96Scaptodrosophilaabdentatahttp://zoobank.org/426657F1-40A2-4E11-BFB1-BBCB8B7A2D42sp. nov.Figs 4A, C, E, 9Material examined.
Holotype. China: ♂ (SCAU, No. 127162), Nanling, Shaoguan, Guangdong, 24°38'N, 112°40'E, alt. 800 m, 3.iii.2004, MF Xu. Paratypes. China: 1♂, 2♀ (SCAU, Nos 127163–65), Nanling, Ruyuan, Guangdong, 24°38'N, 112°40'E, alt. 800 m, 3.iii.2004, MF Xu; 1♂, 2♀ (SCAU, Nos 127166–68), Jianfengling, Ledong, Hainan, 18°41'N, 108°52'E, alt. 700 m, 23.iv.2007, HW Chen; 1♂, 1♀ (SCAU, Nos 127169–70), Beibeng, Motuo, Xizang, 29°19'N, 95°20'E, alt. 780 m, 4.x.2010, YR Su; 2♂ (SCAU, Nos 127171–72), Wangtianshu, Mengla, Yunnan, 21°28'N, 101°38'E, alt. 580 m, 23.iv.2007, HW Chen; 1♂ (SCAU, No. 110918), Muyiji, Ximeng, Yunnan, 22°37'N, 99°35'E, alt. 1100 m, 23.iv.2007, HW Chen; 1♂ (SCAU, No. 110919), Muyiji, Ximeng, Yunnan, 22°37'N, 99°35'E, alt. 1203 m, 30.iv. 2016, YQ Liu; 2♂ (SCAU, Nos 110920, 21), Mengdong, Cangyuan, Yunnan, 23°10'N, 99°13'E, alt. 1323 m, 6.v.2016, YQ Liu.
Diagnosis.
Surstylus with a row of ca 9 long, peg-like prensisetae on caudal margin, and ca 6 setae on outer surface (Fig. 9A, B); paramere leaf-shaped in lateral view, with ca 3 sensillae and a row of fine setae (Fig. 9C, D); aedeagus broadened apically (Fig. 9C, D).
Description.
(♂, ♀) Head (Fig. 4A): Frons yellowish brown. Pedicel brown. First flagellomere yellowish. Facial carina brownish, short, 1/3 length as face.
Thorax (Fig. 4C, E): Mesonotum and scutellum yellowish brown. Acrostichal setulae in ca 8 regular rows. Halter hazel. Wings yellowish.
Abdomen (Fig. 4C, E): Tergites creamy white.
Male terminalia: Epandrium with ca 15 setae near posterior margin and ventral corner on each side (Fig. 9A, B). Hypandrium glabrous (Fig. 9C, D).
Female terminalia (Fig. 8E): Oviscapt with 3 long subapical trichoid ovisensilla, 15 peg-like ovisensilla on each side on ventral margins and 5 trichoid ovisensilla on dorsal.
Facial carina short; paramere finger-like in lateral view, with ca 4 sensillae basally (Fig. 10C, D); aedeagus bifid apically (Fig. 10C, D); gonopods undeveloped (Fig. 10C, D).
Description.
Head (Fig. 4B): Frons yellowish brown. Pedicel yellowish brown. First flagellomere yellowish. Facial carina hazel, short, 1/3 length as face.
Thorax (Fig. 4D, F): Mesonotum and scutellum yellowish. Acrostichal setulae in ca 8 regular rows. Halter hazel. Wings yellowish.
Abdomen (Fig. 4D, F): Tergites yellowish.
Male terminalia: Epandrium with ca 11 setae near posterior margin and ventral corner per side (Fig. 10A, B). Surstylus with ca 10 long, peg-like prensisetae on caudal margin, and ca 3 setae on outer surface (Fig. 10A, B). Hypandrium glabrous (Fig. 10C, D).
Female terminalia (Fig. 10E): Oviscapt with 3 long subapical trichoid ovisensilla, ca 21 peg-like ovisensilla on each side on ventral margins and 5 trichoid ovisensilla on dorsal.
A combination of the Latin words: “platys” + “rhinos”, referring to the flat carina.
Distribution.
China (Yunnan).
AnimaliaDipteraDrosophilidaeAE69324A-94EF-542C-B120-C667BC8FE824Scaptodrosophilaserrateifoliaceahttp://zoobank.org/9DC99991-AE49-4AC7-854B-C6CBC6AC9E88sp. nov.Figs 5A, C, E, 11Material examined.
Holotype. China: ♂ (SCAU, No. 126826), Hesong, Menghai, Yunnan, 21°49'N, 100°06'E, alt. 1900 m, 11.iv. 2011, JM Lu, YR Su, ZF Shao, SJ Yan. Paratypes. China: 1♂ (SCAU, No. 126827), Hesong, Menghai, Yunnan, 21°50'N, 100°06'E, alt. 1900 m, 11.iv.2011, JM Lu, YR Su, ZF Shao, SJ Yan. 1♂ (SCAU, No. 110929), Mengdong, Cangyuan, Yunnan, 23°10'N, 99°13'E, alt. 1323 m, 6.v.2016, YQ Liu.
Diagnosis.
Paramere quadrangle-shaped in lateral view, with ca 3 sensillae medially (Fig. 11C, D); gonopods developed, arm-shaped, with many finely acanthoid processes (Fig. 11C, D); aedeagus beanpod-shaped in lateral view (Fig. 11C, D).
Description.
(♂, ♀) Head (Fig. 5A): Frons yellowish brown. Pedicel yellow. First flagellomere yellowish. Facial carina yellowish, short, 1/2 length as face.
Thorax (Fig. 5C, E): Mesonotum and scutellum dark brown. Acrostichal setulae in ca 8 irregular rows. Halter yellowish brown. Wings hazel.
Abdomen (Fig. 5C, E): First tergite and second tergite yellow. Third to sixth tergites brown.
Male terminalia: Epandrium with ca 19 setae near posterior margin and ventral corner on each side (Fig. 11A, B). Surstylus broad, with ca 10 fine peg-like prensisetae on caudal margin, and numerous setae on outer and inner surfaces (Fig. 11A, B). Hypandrium pubescent near paramedian setae (Fig. 11C, D).
Female terminalia (Fig. 11E): Oviscapt with 1 long subapical trichoid ovisensillum on ventral margins and densely covered with peg-like ovisensilla.
Holotype. China: ♂ (SCAU, No. 110939), Laobiangou, Benxi, Liaoning, 41°04'N, 124°30'E, alt. 575 m, 21.vi.2018, Y Lin. Paratypes. China: 2♂ (SCAU, Nos 110936, 37), Dayangcha, Kuangdian, Liaoning, 41°01'N, 124°37'E. alt. 485 m, 23.vi.2018, L Gong; 2♂ (SCAU, Nos 110938, 40), Laobiangou, Benxi, Liaoning, 41°04'N, 124°30'E, alt. 575 m, 21.vi.2018, Y Lin; 3♂ (SCAU, Nos 110941, 42, 43), Guojiapuzi, Kuangdian, Liaoning, 40°46'N, 124°45'E. alt. 342 m, 26.vi.2018, L Gong.
Diagnosis.
Paramere quadrangle-shaped in lateral view, with ca 3 sensillae medially (Fig. 12A, B); gonopods large, with many finely acanthoid processes (Fig. 12C, D); aedeagus beanpod-shaped in lateral view (Fig. 12C, D).
Description.
(♂) Head (Fig. 5B): Frons yellowish brown. Pedicel yellow. First flagellomere yellowish brown. Arista weak. Facial carina yellowish, developed, 1/2 length as face.
Thorax (Fig. 5D, F): Mesonotum and scutellum yellowish. Acrostichal setulae in ca 8 irregular rows. Halter yellowish brown. Wings light brown and appreciably hyaline.
Abdomen (Fig. 5D, F): Tergites yellowish.
Male terminalia (Fig. 12): Epandrium with ca 23 setae near posterior margin and ventral corner on each side (Fig. 12A, B). Surstylus broad, with ca 12 fine peg-like prensisetae on caudal margin, and numerous setae on outer and inner surface (Fig. 12A, B). Hypandrium pubescent near paramedian setae (Fig. 12C, D).
Male terminalia: Epandrium with ca 13 setae near posterior margin and ventral corner on each side (Fig. 13A, B). Surstylus small with ca 9 peg-like prensisetae on caudal margin, and ca 6 setae on outer and inner surface (Fig. 13A, B). Hypandrium glabrous (Fig. 13C, D).
Female terminalia (Fig. 13E): Oviscapt with 3 long subapical trichoid ovisensilla, ca 19 peg-like ovisensilla on each side on ventral margins and 4 trichoid ovisensilla on dorsal.
Scaptodrosophilariverata (Singh & Gupta, 1977) ♂. A Epandrium, surstylus and cercus (posterior view) B surstylus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilapuncticeps (Okada, 1956) ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilaabdentata sp. nov. ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagu, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilaplatyrhina sp. nov. ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilaserrateifoliacea sp. nov. ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilasinuata sp. nov. ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view). Scale bars: 0.1 mm.
Scaptodrosophilatanyrhina sp. nov. ♂. A Epandrium, surstylus and cercus (posterior view) B epandrium, surstylus and cercus (lateral view) C hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (ventral view) D hypandrium, aedeagus, aedeagal apodeme, paramere and gonopods (lateral view) E oviscapt (lateral view). Scale bars: 0.1 mm.
We thank the reviewer Prof. PM O’Grady (Cornell University, USA) for the detailed corrections, and all the members of our laboratory (SCAU) for helping us in all the fieldwork. The parts of DNA sequences were provided by the Southern China DNA Barcoding Center, Kunming Institute of Zoology, China. This study was supported by the National Natural Science Foundation of China (Nos. 31672321, 31093430).
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