Corresponding author: Ying-Yong Wang (
Academic editor: A. Crottini
Wan H, Lyu Z-T, Qi S, Zhao J, Li P-P, Wang Y-Y (2020) A new species of the
As the type genus of the family
Based on morphological comparisons and geographical conditions,
During herpetofaunal surveys in the Luoxiao Range, which is situated between the Jiangxi and Hunan provinces (Fig.
Collecting localities of
Eight unnamed specimens were collected from multiple localities of Jiangxi and Hunan provinces. All specimens were fixed in 10% buffered formalin, later transferred to 70% ethanol, and deposited in the
The morphological description follows the consistent definition by
A total of 56 muscular samples of
Localities, voucher information, and GenBank numbers for all samples of the genus
ID | Species | Localities | Voucher no. | 16S |
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China: Jiangxi: Mt Guanshan * |
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China: Jiangxi: Mt Guanshan * |
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China: Jiangxi: Mt Wugong |
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China: Jiangxi: Mt Wugong |
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China: Hunan: Mt Mufu |
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China: Hunan: Mt Dawei |
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China: Hunan: Mt Dawei |
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China: Hunan: Mt Dawei |
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China: Hunan: Mt Dawei |
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China: Heilongjiang: Taiyang Island | SYNU 11100267 |
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China: Liaoning, Zhangwu | SYNU 11100268 |
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Germany: Lower Saxony | No voucher |
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China: Xinjiang: 47tuan | KIZ XJ0251 |
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China: Sichuan: Zhaojue * |
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China: Sichuan: Zhaojue * |
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China: Shaanxi: Huxian * | KIZ RD05SHX01 |
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China: Henan: Mt Yawu |
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China: Henan: Mt Yawu |
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South Korea | MMS 223 |
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China: Shandong: Mt Kunyu | SYNU 08090641 |
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China: Shandong: Mt Culai * | KIZ SD080501 |
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China: Shandong: Mt Culai * | SYNU 08090549 |
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China: Jiangxi: Mt Wugong |
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China: Jiangxi: Shanggao |
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China: Jiangxi: Mt Meiling |
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China: Anhui: Dabie Mountains area * | AHU 2016R001 |
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Russia: Primorye: Khasanskii | MSUZP-IVM-1d |
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China: Jilin: Mt Laoling | SYNU 11070163 |
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China: Hunan: Mt Yangming * |
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China: Hunan: Mt Bamian |
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China: Hunan: Mt Badagong |
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China: Hunan: Mt Yunshan |
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China: Hunan: Mt Xuefeng |
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China: Hunan: Suining |
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China: Hunan: Mt Shunhuang |
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China: Guizhou: Mt Leigong |
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China: Guizhou: Mt Fanjing |
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China: Jiangxi: Mt Jinggang |
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China: Jiangxi: Mt Qiyun |
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China: Guangxi: Longsheng |
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China: Guangxi: Mt Dupangling |
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China: Guangdong: Renhua |
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China: Liaoning: Huanren * | SYNU 07040035 |
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China: Liaoning: Huanren * | y-d20130058 |
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Japan: Isumi-shi: Chiba Prefecture | KIZ YPX11775 |
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Japan: Isumi-shi: Chiba Prefecture | NNRj |
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China: Hunan: Jiemuxi * |
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China: Hunan: Jiemuxi * |
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Vietnam: Lam Dong: Loc Bao | ABV 00203 |
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China: Qinghai: Qinghai Lake * | KIZ CJ06102001 |
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China: Sichuan: Hongyuan |
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China: Sichuan: Hongyuan |
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China: Taiwan:Taipei * | Not given |
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China: Taiwan: Miaoli: Xiangtianhu | NMNS 15022 |
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China: Fujian: Mt Yashu |
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China: Jiangxi: Mt Magu |
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China: Jiangxi: Mt Qiyun |
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China: Jiangxi: Mt Jiulian |
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China: Jiangxi: Mt Sanbai |
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China: Jiangxi: Suichuan |
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China: Guangdong: Renhua |
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China: Guangdong: Mt Nankun |
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China: Guangdong: Pu’ning |
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China: Guangdong: Mt Tonggu |
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China: Guangdong: Yingde |
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China: Guangxi: Mt Maoershan * | SYNU 08030061 |
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China: Guangxi: Mt Maoershan * | SYNU 08030062 |
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China: Henan: Luanchuan * | KIZ 047452 | / |
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China: Henan: Luanchuan * | KIZ 047393 | / |
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China: Sichuan: Mt Emei * |
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China: Sichuan: Mt Emei * |
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China: Hunan: Mt Tianping * |
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China: Hunan: Mt Tianping * |
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China: Sichuan: Hongya: Zhangcun * | SCUM 0405190CJ |
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China: Sichuan: Hongya: Zhangcun * | KIZ YP06057 |
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China: Taiwan: Kaohsiung * | SCUM 0405175CJ |
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China: Sichuan: Zhaojue |
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China: Zhejiang: Hangzhou | SYNU 08040100 |
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China: Zhejiang: Zhenhai * | KIZ 0803271 |
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China: Zhejiang: Fenghua |
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China: Jiangxi: Mt Tongbo |
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China: Jiangxi: Mt Guanshan |
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China: Jiangxi: Mt Yangjifeng |
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For phylogenetic analyses, 26 additional sequences from all known Chinese congeners of the subgenus
The unnamed specimens from Jiangxi and Hunan are assigned to the
The new species differs from
The new species differs from
The new species further differs from
From
The
Bayesian inference and maximum-likelihood phylogenies based on mitochondrial 16S and
The
Therefore, based on the significant morphological differences and phylogenetic divergence, these specimens from Jiangxi and Hunan represent a distinct evolutionary lineage and are described as a new species,
Morphological features of the adult male holotype
Seven adult specimens. Females
The specific name
Jiuling Mountains Brown Frog (in English), Jiu Ling Shan Lin Wa (九岭山林蛙 in Chinese)
Forearms 0.19 of
Tibia 0.63 of
Dorsal skin smooth with sparse tiny granules; several small tubercles on flank; supratympanic fold absent; dorsolateral fold distinct and thin, extending straight from posterior margin of upper eyelid to above groin; several tiny granules on the skin of loreal and temporal regions; ventral surface smooth, large flattened tubercles densely arranged on the rear of thigh and around vent.
In life, dorsal surface yellowish brown with few black spots; black speckles forming a linear stripe between eyelids; dorsolateral fold intermittently edged with black on two sides; loreal region yellowish; temporal region yellowish, slightly tinged with grey; tiny granules on loreal and temporal regions reddish; dorsal forelimbs and hindlimbs reddish with indistinct greenish grey transverse bars. Throat yellowish; chest and belly creamy white; ventral surface of forelimbs and hindlimbs flesh color; nuptial pad creamy white; tubercles around vent yellowish.
In preservative, dorsal surface turns grey with black spots and light grey patches; limbs taupe with brown transverse bars. Ventral surface white, with greyish mottling on throat and belly; ventral surface of limbs beige; hands and toe webs dark grey.
Measurements of type series specimens are given in Table
Measurements (in mm) of the type series of
Sex | Male | Male | Male | Male | Female | Female | Female | Female |
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57.8 | 51.6 | 48.3 | 49.1 | 57.5 | 48.4 | 49.4 | 48.2 |
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21.6 | 19.3 | 18.4 | 17.7 | 22.3 | 18.9 | 18.2 | 19.4 |
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18.4 | 17.0 | 15.7 | 12.6 | 19.3 | 16.1 | 15.5 | 15.8 |
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7.8 | 7.5 | 7.3 | 7.5 | 8.1 | 7.1 | 7.2 | 7.4 |
IN | 4.1 | 3.8 | 3.5 | 3.2 | 4.2 | 3.6 | 4.1 | 4.1 |
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3.4 | 2.7 | 3.0 | 3.1 | 3.6 | 3.4 | 3.3 | 3.3 |
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6.3 | 5.5 | 5.2 | 5.0 | 5.2 | 4.4 | 4.7 | 4.6 |
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4.6 | 3.5 | 3.3 | 3.9 | 4.5 | 3.7 | 3.2 | 3.4 |
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1.9 | 2.0 | 1.7 | 2.0 | 1.7 | 1.7 | 1.7 | 1.6 |
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15.0 | 14.0 | 14.0 | 12.5 | 15.3 | 13.9 | 13.2 | 12.5 |
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11.2 | 11.2 | 9.0 | 9.3 | 10.5 | 10.4 | 10.9 | 10.1 |
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50.7 | 44.2 | 41.7 | 42.8 | 47.6 | 43.8 | 44.3 | 41.1 |
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36.6 | 30.4 | 29.5 | 30.7 | 36.1 | 31.8 | 31.8 | 29.0 |
Variations of
Currently,
All recognized species of the subgenus
Within the
The discovery of
We thank the Hunan Badagongshan National Nature Reserve, Fujian Yashushan Nature Reserve, Jian Wang, Chun-Lin Liao, Hai-Long He, Zhao-Chi Zeng, Yu-Long Li, and Zheng-Yan Zhou for their help with the fieldwork. We thank Zhen-Hua Liu, Zi-Chen Zhou, Yang Chen, and Robert Forsyth for their help in polishing the manuscript. We thank Angelica Crottini and two anonymous reviewers for their helpful suggestions on our work. This work was supported by the Project of Comprehensive Scientific Survey of Luoxiao Mountains Region of Ministry of Science and Technology, China (no. 2013FY111500), the Project of Scientific Investigation on the Amphibian, Reptilian and Avian Animals in Jiangxi Jiulianshan National Nature Reserve, the Project of Animal Diversity Survey and Monitoring System Construction of Guangdong Shimentai National Nature Reserve, and the Project of Survey of Terrestrial Vertebrate Diversity in Guangdong Danxiashan National Nature Reserve.
Tables S1, S2
Tables for genetic distances