Description of Ortheziolamameti tranfagliai new species (Hemiptera, Coccoidea, Ortheziidae) from India

Abstract This paper describes a new Ortheziolamameti species from the Oriental region (India), namely Ortheziolamameti tranfagliai Konczné Benedicty, sp. n. The examined material was extracted from forest litter from India, using Berlese funnels. With this new species the genus Ortheziolamameti now includes six species. An identification key and distribution map are provided.

wax plates, and a narrow band in midline of the dorsum, (ii) 3-segmented antennae (the size, shape, number, and type of setae highly variable), (iii) eye stalks protruding, thumb-like, fused with sclerotized area at base of antennae, sometimes called pseudobasal antennal segment, (iv) tarsi without digitules and (v) abdominal spiracles ventral on anterior segments, with at least one present on each side of abdominal segments I, II, or III; if present, posterior abdominal spiracles located on dorsum near anal ring surrounded by a cluster of multilocular disc pores. Ortheziolinae species are mainly found in the soil and distributed in the Palaearctic, Oriental and Ethiopian regions, and their host plant relationships are not well-known. In the subfamily there are four tribes, namely Ortheziolamametini Kozár, Ortheziolini Kozár, Matileortheziolini Kozár and Ortheziolacoccini Kozár. Ortheziolamametini is characterized by having: (i) dorsum covered by wax plates, those in the middorsum being triangular, (ii) midthorax of venter without wax plates, and (iii) two spine bands inside the ovisac band. Species of Ortheziolamametini are distributed in the Ethiopian, Oriental and Palaearctic Regions. Although the genus is a typical member of the subfamily Ortherziolinae, it somewhat resembles Arctorthezia of subfamily Ortheziinae, known mainly in the northern part of the Holarctic region, in having the triangular wax plates on the middorsum. The genus includes 5 species, namely O. guineensis (Morrison, 1954), O. loebli (Richard, 1990), O. kosztarabi (Kozár & Miller, 2000), O. maeharai Tanaka &Amano, 2007, andO. taipensiana Shia &Kozár, 2004;  In the present paper one new Ortheziolamameti species is described from the Oriental region (India). An identification key, distribution map and new additional locality records for the currently known Ortheziolamameti species are provided.

Material and methods
The specimens described and recorded in this study were all collected using soil and litter sampling devices, and extracted by Berlese funnel. The samples are preserved in the Muséum d'Histoire Naturelle de Genève (MHNG) collection.
Specimens were prepared for light microscopy using the slide-mounting method discussed by Kosztarab and Kozár (1988). The morphological terminology used follows Kozár (2004), while the key was adopted from Kozár (2004) and Tanaka and Amano (2007).
The digital images of unmounted females were made with a Canon Eos400D camera and an MBC-10 stereomicroscope, and focus-stacking was processed by Combin-eZP software (Hadley 2010). All type material of the new species are deposited in the Muséum d'Histoire Naturelle de Genève (MHNG).
All measurements and counts were taken from all the material available and the values are given as a range for each character.

Results and discussion
Genus Ortheziolamameti Kozár, 2004: 483. Type species. Ortheziolamameti guineensis Kozár, 2004, 484. Diagnosis of genus. Dorsum of intact adult female covered with irregular and triangular shell-like wax plates; ventral thoracic wax plates around the appendages and on margin, thorax medially lacking wax plates; wax cover corresponding to wax plates on slide-mounted specimens on both sides (Kozár 2004) (Fig. 1).
Slide-mounted adult female with 3-segmented antenna; third antennal segment with slender apical seta, flagellate sensory seta and small subapical seta; second segment with 1 sensory pore. Eye stalk protruding, thumb-like, fused with base of antenna. Legs well developed; leg setae robust, spine-like; trochanter and femur fused, tibia and tarsus fused; tibia with 1 sensory pore and at least 1 fleshy sensory seta; tarsus without tarsal digitules; claw digitules hair-like, claw without a denticle. Labium 1 segmented, with many setae; with 3 long setae near apex of labium, setae very close together, all situated in a single setal socket. Anal ring situated in a fold of derm on dorsal surface, ring bearing 6 setae. Sclerotized plate present on dorsum anterior to anal ring, wider than long. Thumb-like pores forming a cluster on each side of anal ring. Abdominal spiracles in centre of multilocular disc pore cluster present laterad to anal ring (Kozár 2004).
Distribution. The six Ortheziolamameti species are distributed in the Ethiopian, Oriental and Palaearctic regions (Fig. 3). For detailed distribution data of previously known five species, see ScaleNet (Ben-Dov et al. 2013). New locality records for several Ortheziolamameti species were discovered during the study of the MHNG collection, which are listed below. Paratypes. 3 adult females, 1 specimen on same slide as holotype, 2 specimens on a separate slide, same data as holotype.

Key to species of Ortheziolamameti
Description. Unmounted female. Not seen.
Quadrilocular pores 3 µm in diameter, scattered in the ovisac band. Multilocular disc pores with 8-12 loculi around perimeter, one loculus in central hub; 7-8 µm in diameter; present near anterior edges of spine bands, scattered along lateral edge of each ovisac band. Abdominal spiracles present anterior of ovisac band; without sclerotized vestibule.
Dorsum. Wax plates covering the whole surface; mediolateral thoracic plates large (plates 3, 5 and 6), covering most of mediolateral thoracic areas; medial area without wax plates, this area with 3 triangular wax plates (plates 8, 9 and 10). Spines at margin of wax plate 4, each 12-14 µm long, in middle of wax plates 18-21 µm long; spines apically capitate. Setae present in marginal clusters near posterior edges of marginal wax plates (plates 2 and 4), with 3 or 4 setae laterad of each thoracic spiracle, 27-30 µm long; also present in very small numbers on other wax plates. Quadrilocular pores 3 µm in diameter, scattered, mainly along margins of wax plates. Multilocular disc pores present in a cluster near anal ring, near the sclerotized plate, and on the margin at the level of ventral thoracic spiracles. Sclerotized plate 80-120 µm long, 265-330 µm wide. Anal ring with two complete rows of round pores (4-5 µm in diameter); three pairs of anal  ring setae each 45-21 µm long; anal ring 43-53 µm wide, 43-53 µm long. Thumb-like pores forming a cluster on each side of anal ring, each 5-8 µm long. Modified pores (quadrilocular pores) 3-4 µm long, scattered on surface. The abdominal spiracle is located in centre of the multilocular disc pore clusters laterad to anal ring.
Ecology. Host plants. Unknown. Collected from forest litter. Distribution. India. Etymology. This species is named after the Italian coccidologist Dr. Antonio Tranfaglia.
Comments. Ortheziolamameti tranfagliai sp. n. can be recognized by the following combination of characters: (i) having hair-like setae on antennal segments, (ii) having two spine bands in the ovisac area and (iii) lacking multilocular disc pores around vulva. O. tranfagliai is similar to O. loebli in having hair-like setae on antennae and lacking multilocular disc pores around vulva, but differs from O. loebli in the following characters (those of O. loebli in brackets), (i) plates 13, 17 and 18 resembling very small groups of spines (plates 13, 17 and 18 complete); (ii) without cluster of spines between hind legs (with large cluster of spines between hind legs) and (iii) second spine band in ovisac area in a sparse row (in a complete row).