RT Journal Article T1 Systematics of the Osteocephalus buckleyi species complex (Anura, Hylidae) from Ecuador and Peru JF ZooKeys JO ZK FD Pensoft Publishers DO 10.3897/zookeys.229.3580 VO 229 A1 Ron, Santiago R. A1 Venegas, Pablo J. A1 Toral, Eduardo A1 Read, V. Morley A1 Ortiz, Diego A1 Manzano, Andrea YR 2012 UL https://doi.org/10.3897/zookeys.229.3580 AB We present a new phylogeny, based on DNA sequences of mitochondrial and nuclear genes, for frogs of the genus Osteocephalus with emphasis in the Osteocephalus buckleyi species complex. Genetic, morphologic, and advertisement call data are combined to define species boundaries and describe new species. The phylogeny shows strong support for: (1) a basal position of O. taurinus + O. oophagus, (2) a clade containing phytotelmata breeding species, and (3) a clade that corresponds to the O. buckleyi species complex. Our results document a large proportion of hidden diversity within a set of populations that were previously treated as a single, widely distributed species, O. buckleyi. Individuals assignable to O. buckleyi formed a paraphyletic group relative to O. verruciger and O. cabrerai and contained four species, one of which is O. buckleyi sensu stricto and three are new. Two of the new species are shared between Ecuador and Peru (O. vilmae sp. n. and O. cannatellai sp. n.) and one is distributed in the Amazon region of southern Peru (O. germani sp. n.). We discuss the difficulties of using morphological characters to define species boundaries and propose a hypothesis to explain them.