Guilotes, a new genus of Coelotinae spiders from Guangxi Zhuang Autonomous Region, China (Araneae, Agelenidae)

Abstract A new genus of the subfamily Coelotinae F.O. Pickard-Cambridge, 1893, Guilotes Z. Zhao & S. Li, gen. n. from China is described, as well as four new species: G.ludiensis Z. Zhao & S. Li, sp. n. (♂♀, type species), G.qingshitanensis Z. Zhao & S. Li, sp. n. (♂♀), G.xingpingensis Z. Zhao & S. Li, sp. n. (♂♀) and G.yandongensis Z. Zhao & S. Li, sp. n. (♀). The DNA barcodes of all species are documented for future use.


Introduction
The spider subfamily Coelotinae (Araneae, Agelenidae) comprises 743 valid species belonging to 30 genera (World Spider Catalog 2018;Li and Quan 2017). Coelotinae are recorded only in the Nearctic, Palearctic, and Indo-Malaya regions with 89% of the species distributed in Asia, 7% in Europe, and 4% in North America. New coelotine genera and species had been recently discovered (Chen et al. 2015a(Chen et al. , b, 2016aZhang and Marusik 2016;Zhang et al. 2016a, b;Zhao and Li 2016;Komnenov 2017;Okumura 2017;Quasin et al. 2017;Zhu et al. 2017;Li et al. 2018a, b). Zhao and Li (2017) studied the evolutionary history and biogeography of Coelotinae using molecular data (8 genes, ~ 6.5 kb) on 18 genera and 286 coelotine species. The well resolved phylogeny of coelotine spiders promoted the new taxa erecting and taxonomic revisions (Chen et al. 2016b;Zhao and Li 2016;Li et al. 2018a, b). So far, there are 387 coelotine species (about 52% of the total) from 24 genera reported from China. However, in the karst regions of China, there are still many poorly known species with unusual characters which are not belonging to any known coelotine genera (World Spider Catalog 2018).
In this study, Guilotes Z. Zhao & S. Li, gen. n. is proposed to include four new species. The new genus was confirmed by the phylogenetic framework of coelotine spiders . The molecular topologies supported Guilotes as a monophyletic group in Guangxi Coelotes groups. The new species of Guilotes are compared with those of the morphological similar genus Notiocoelotes Wang, Xu & Li, 2008. All specimens of Guilotes were collected from karst regions in southern China.

Materials and methods
Specimens were examined with a LEICA M205C stereomicroscope. Photographs were captured with an Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted either on an Olympus SZX12 dissecting microscope or on an Olympus BX51 compound microscope. Photos from multiple focal ranges were combined using Helicon Focus (Version 3.10) photo stacking software. Female epigyne and male palp were dissected form the body to be proper examined. The epigyne was removed and treated in a warmed 10% potassium hydroxide (KOH) solution before study. Measurements were obtained with a LEICA M205C stereomicroscope and are given in millimeters. Eye diameters were measured as the maximum diameter from either dorsal or frontal views. Leg measurements are given as: total length (femur, patella + tibia, metatarsus, tarsus). Images of the male left palp are presented. Terminology of the structures follows Wang (2002), Chen et al. (2015b) and Zhang et al. (2016b).
References to figures in the cited papers are listed in lowercase (figure or figs); figures from this paper are noted with an initial capital (Figure or Figs). Abbreviations used in the text and figures: A epigynal atrium; ALE anterior lateral eye; ALE-PLE distance between ALE and PLE; AME anterior median eye; AME-ALE distance between AME and ALE; AME-AME distance between AME and AME; AME -PME distance between AME and PME; C conductor; CD copulatory duct; CF cymbial furrow; DNA barcodes were also obtained for the species delimitation and matching. A partial fragment of the mitochondrial cytochrome oxidase subunit I (CO1) gene was amplified and sequenced for all species using the primers LCO1490-oono (5'-CWACAAAYCATARRGATATTGG-3') and C1-N-2776 (5'-GGATAATCA-GAATANCGNCGAGG-3'). For additional information on extraction, amplification and sequencing procedures, see Zhao and Li (2017). All sequences were analyzed using BLAST and are deposited in GenBank. The accession numbers are provided in Table 1. Etymology. The generic name is derived from the pinyin word "Gui", referring to the Guangxi Zhuang Autonomous Region (Gui is a short name for Guangxi) where the genus is distributed, and "-lotes" as part of Notiocoelotes, which is similar to the new genus. The gender is masculine.
Diagnosis. The males of the genus Guilotes are similar to those of the genus Notiocoelotes by cymbial furrow long (Figs 1C, 3C, 5C) and embolus long and filiform (Figs 1B, 3B, 5B), but can be distinguished by the chelicerae with 5-6 promarginal and five retromarginal teeth; the presence of a patellar apophysis ( Figure 1C) and conductor short not reaching the embolus base ( Figure 1B). The females of the genus Guilotes are similar to those of the genus Notiocoelotes by atrium oval and posterior (   Natural history. All species of this genus were collected from moist caves with soluble rock. Comments. The new genus was supported as monophyletic within the Guangxi Coelotes group (Zhao and Li 2017: 993, figure 3 -see ZZ391, ZZ392, ZZ890, and ZZ892). Males and females of each species were collected from the same caves and double-checked using DNA barcoding.
The divergence time analyses showed the split of Guilotes gen. n. and other close related clades early than 30 million years ago (Zhao and Li 2017: figure 3, S8). Guilotes and Notiocoelotes are very similar in morphology and present similar distribution areas (Wang et al. 2008, Liu et al. 2010, Zhang et al. 2016b; thus, it is meaningful to distinguish between them. Distribution. Guangxi Zhuang Autonomous Region, China ( Figure 8).  Figure 1C). Differ from G. qingshitanensis sp. n. by the straight and short conductor with a LC and embolus beginning at 5 o'clock position ( Figure 1B). Females can be distinguished from G. xingpingensis sp. n. by the epigynal teeth located in the middle of the atrial lateral margins (Figure 2A), copulatory ducts concave laterally and narrow fertilization ducts ( Figure 2B). Differ from G. qingshitanensis sp. n. by the presence of epigynal teeth ( Figure 2A) and sac-like of copulatory ducts (Figs 2B, 4B). Differ from G. yandongensis sp. n. by locations of epigynal teeth near the atrial lateral margins, atrium twice wider than long (Figure 2A), copulatory ducts ending horizontally and narrow fertilization ducts ( Figure 2B).

Guilotes yandongensis
Diagnosis. Females of Guilotes yandongensis sp. n. can be differ from G. qingshitanensis sp. n. by having the epigynal teeth ( Figure 7A) and wide fertilization duct ( Figure 7B); they differ from G. ludiensis sp. n. and G. xingpingensis sp. n. by the copulatory ducts with folded lateral margin ( Figure 7B).