Phylogenetic reconstruction of Lithobates warszewitschii relationships between Costa Rican and Panamanian populations using concatenated alignments of CO1 and 16S. Node support values (posterior probabilities) and percentages calculated from 1000 bootstrap replicates are annotated at nodes. Samples collected in different localities are represented by different colours: individuals from Área de Conservación Guanacaste (ACG; Cacao, Caribe, Maritza, Pitilla, and San Gerardo) highlighted in red, individuals from Brewster highlighted in purple, and individuals from El Copé highlighted in orange. Sample information can be found in Table 2. Separate trees were constructed in Mr. Bayes and RAxML using a GTR model of molecular evolution, both with similar topologies, therefore node supports were included in a single tree. Scale of branch lengths is in nucleotide substitutions per site.