A new earless species of Poyntonophrynus (Anura, Bufonidae) from the Serra da Neve Inselberg, Namibe Province, Angola

Abstract African pygmy toads of the genus Poyntonophrynus are some of the least known species of African toads. The genus comprises ten recognized species endemic to sub-Saharan Africa, five of which are restricted to southwestern Africa. Recent field research in Angola provided new material for three species of Poyntonophrynus, including a morphologically distinctive population from the Serra da Neve Inselberg. Based on a combination of external morphology, high-resolution computed tomography scanning, and molecular phylogenetic analysis, the Serra da Neve population is described as new species that is nested within the genus. The most striking character that differentiates the newly described species from its congeners is the lack of a tympanic middle ear, a condition common in the family Bufonidae, but so far not known for Poyntonophrynus. The description of this new species from southwestern Angola reinforces the biogeographic importance of the region and further suggests that southwestern Africa is the cradle of diversity for this genus.


Introduction
African pygmy toads of the genus Poyntonophrynus Frost et al., 2006, are a group of 'true' toads (family Bufonidae) that are endemic to sub-Saharan Africa Carruthers 2009, 2017). Similarities among what Poynton (1964) termed the Bufo 'vertebralis group' were recognized long before a genus was erected for them (Boulenger 1905, Mertens 1955, Poynton 1964, Tandy 1972, Poynton and Haacke 1993. Likewise, their similarity to those species today placed in the genus Mertensophryne has also long been acknowledged (Tandy 1972, Poynton 1996, Frost et al. 2006. However, the diversity and relationships of taxa in this group have remained contentious and confusing. Tandy (1972, Tandy andKeith 1972) revised Poynton's (1964) 'vertebralis group' by excluding Bufo taitanus but adding both B. lughensis and B. parkeri due to similarities with B. dombensis and B. fenoulheti, respectively. The recognized diversity of this group has remained largely static since the description of B. grandisonae by Poynton and Haacke (1993) and the short summary by Poynton (1996). Frost et al. (2006) recognized the genus Poyntonophrynus for a group of ten species that have typically been referred to as the 'vertebralis group' (Poynton 1964, Tandy 1972, Poynton and Broadley 1988, Poynton 1996. However, this was based solely on the results of Cunningham and Cherry (2004), who sampled three of these ten species finding that they form a clade exclusive of other African bufonids. Van Bocxlaer et al. (2010) first confirmed the long-suspected close phylogenetic relationship between the species recognized by Frost et al. (2006) as Mertensophryne and Poyntonophrynus. This result was expanded upon recently by Liedtke et al. (2017) who included new data for seven of the ten species included by Frost et al. (2006) in Poyntonophrynus.
There are currently ten recognized species in the genus Poyntonophrynus, all of which are small terrestrial toads with inconspicuous parotoid glands and lacking a tarsal fold. One East African species, however, Poyntonophrynus lughensis (Loveridge, 1932), was recently demonstrated to be more closely related to Mertensophryne (Liedtke et al. 2017). The remaining nine species are found in sub-Saharan Africa, with most of the diversity concentrated in Angola and Namibia. While the life history of the majority of these species is not well known, at least some species breed in shallow rocky pools and metamorphose relatively quickly (within ~3 weeks ;Power 1926, Channing 1976, Channing and Vences 1999, Channing et al. 2012). The currently recognized species include the following: P. beiranus (Loveridge, 1932a), distributed in two populations, one in the coastal areas of Mozambique and another in southwestern and central Zambia; P. damaranus (Mertens, 1954), known from northwestern Namibia, but potentially also occurring in southwestern Angola; P. dombensis (Bocage, 1895), extending from southwestern Angola to central Namibia; P. fenoulheti (Hewitt & Methuen, 1912), with five described subspecies (fenoulheti, obtusum, albiventris, rhodesianus and grindleyi, all currently synonymized with the nominotypical form -see Frost 2017), occurring in western Mozambique, northeastern South Africa, Zimbabwe, the Caprivi Strip of Namibia, and portions of eastern and northern Botswana and southern Zambia; P. grandisonae (Poynton & Haacke, 1993), endemic to southwestern Angola (Namibe Province); P. hoeschi (Ahl, 1934), endemic to the central-western regions of Namibia; P. kavangensis (Poynton & Broadley, 1988), occurring in the Kavango River basin, in western Zimbabwe, northern Botswana, northern Namibia and southern Angola; P. parkeri (Loveridge, 1932b), restricted to northern Tanzania and southern Kenya; and P. vertebralis (Smith, 1848), the type species of the genus, occurring in central South Africa (Figure 1). Our current knowledge of the diversity of Poyntonophrynus remains limited and some species, such as P. grandisonae and P. parkeri, have not been documented since their original descriptions. Notably, five species of Poyntonophrynus occur in southwestern Africa, with four occurring in Namibia (P. dombensis, P. hoeschi, P. damaranus and P.  Figure 2) and other four in southern Angola (P. dombensis, P. fenoulheti, P. grandisonae, and P. kavangensis). It is possible that other species of Poyntonophrynus also occur in Angola (Figure 2). Despite being among the better explored regions in Angola, there are still new distributional records and undescribed species of reptiles and amphibians being discovered in the coastal lowlands of Namibe and Benguela provinces (Conradie et al. 2012. These included the Slender Feather-tailed Gecko, Kolekanos plumicaudus (Haacke, 2008), the sole representative of the endemic genus Kolekanos Heinicke, Daza, Greenbaum, Jackman & Bauer, 2014;Haacke's Sand Lizard, Pedioplanis haackei Conradie, Measey, Branch &Tolley, 2012, andHuntley's Sand Lizard, P. huntleyi Conradie, Measey, Branch &Tolley, 2012;and the Kaokoveld Girdled Lizard, Cordylus namakuiyus Stanley, Ceríaco, Bandeira, Valério, Bates & Branch, 2016. Namibe Province also harbors four other endemic lizard species (Ansorge's Gecko, Afrogecko ansorgii (Boulenger, 1907), the Angolan Thick-Toed Gecko, Pachydactylus angolensis Loveridge, 1944, the Angolan Namib Day Gecko, Rhoptropus taeniostictus Laurent, 1964, andBogert's Dotted Blind Dart Skink, Typhlacontias punctatissimus bogerti Laurent, 1964) and the poorly known endemic Grandison's Pygmy Toad, Poyntonophrynus grandisonae. Some species known to occur in neighboring Namibia have also recently been found in Namibe Province, including the Large-Scaled Thick-Toed Gecko, Pachydactylus scutatus Hewitt, 1927, andthe Damaraland Sand Frog, Tomopterna damarensis Dawood &Channing, 2002, previously known only from its type locality in Khorixas, Namibia , Heinicke et al. 2017a.

kavangensis;
During recent field research in Angola, we collected specimens representing several species of Poyntonophrynus. These include the second record for Angola of P. dombensis, the first specimens of P. grandisonae since the type series, and one undescribed species, with which this paper deals (Marques 2015). On Serra da Neve, an isolated inselberg in northern Namibe Province and the second highest peak in Angola (2489 m a.s.l. fide Pereira 1977), we collected specimens of a unique taxon of small toad. These specimens lack an externally visible ear, but otherwise are morphologically similar to species in the genus Poyntonophrynus. Despite considerable differences in size and shape, all described species of this genus have a visible tympanum, thus suggesting that the Serra da Neve population represents an unknown taxon. Based on our molecular phylogenetic and morphological studies, we describe this population from Serra da Neve as a new species of pygmy toad. We conclude by briefly discussing the biogeographic importance of the Serra da Neve Inselberg and the arid lowland areas of southwestern Angola.

Specimens examined
Specimens collected for this study were preserved in 10% neutral buffered formalin in the field and transferred to 70% ethanol for storage. Liver tissue was removed before formalin fixation and preserved in either 95% ethanol or RNA Later. Specimens are deposited in the Florida Museum of Natural History at the University of Florida (UF; Gainesville, USA), the California Academy of Sciences (CAS; San Francisco, USA), the Instituto Nacional da Biodiversidade e Áreas de Conservação (INBAC; Kilamba-Kiaxi, Angola), and the Museu Nacional de História Natural e da Ciência (MUHNAC; Lisboa, Portugal). We made comparisons with specimens in the Museum of Comparative Zoology of Harvard University (MCZ; Cambridge, USA) as well as consulted data presented in the descriptions of Poyntonophrynus species and other relevant works (e.g., Poynton and Broadley 1988, Channing 2001, du Preez and Carruthers 2009, 2017.

Molecular methods
Portions of the mitochondrial 16S gene and nuclear RAG-1 gene were sequenced for newly collected specimens of Poyntonophrynus grandisonae and the undescribed samples from Serra da Neve. Methods of tissue extraction, PCR, and sequencing follow Heinicke et al. (2017b). DNA was extracted from preserved liver samples using the Qiagen DNeasy Blood and Tissue kit following the manufacturer's protocol. PCR reactions were performed for 40 cycles at an annealing temperature of 50 °C, using the primer pair 16SA (5'-CGCCTGTTTATCAAAAACAT-3') and 16SB (5'-CCG-GTCTGAACTCAGATCACGT) (Palumbi et al. 1996) and the primer pair RAG1-C (5'-GGAGATGTTAGTGAGAARCAYGG) and RAG1-E (5'-TCCGCTGCATTTC-CRATGTCRCA) (Biju and Bossuyt 2003). Following PCR, DNA was purified with Axygen AxyPrep magnetic beads, and purified DNA was sequenced at the University of Michigan DNA sequencing core.
Sequence assembly was performed using BioEdit 7.0.5.3 (Hall 1999), and new sequences were deposited in GenBank (Table 1). The new sequences were then integrated into a data set consisting of all Poyntonophrynus, Mertensophryne, and Capensibufo sequences used in Liedtke et al. (2017), which include 16S and RAG-1, but also sequences of 12S, CO1, ND2, and CXCR4 (Table 1). All previously sequenced species of Poyntonophrynus and Mertensophryne were included in that particular data set. Although Liedtke et al. (2017) provided the first genetic data for several species of Poyntonophrynus, there are no voucher specimens associated with these data to compare to our specimens (J. Streicher, pers. comm.). Sequences of each gene were aligned using ClustalX (Larkin et al. 2007). Following alignment, the best performing partition scheme was identified using PartitionFinder 2.1.1 (Lanfear et al. 2012), treating each gene and each codon position of the protein-coding genes as potentially unique character sets, and performing a greedy search on the 56 commonly used models of evolution.

Species
Specimen RAG1 position 3 (TrN+G). Branch support was assessed with 1000 bootstrap replicates. The resulting tree was rooted using Capensibufo, which recent published phylogenies suggest to be closely related to but outside of a clade containing Poyntonophrynus and Mertensophryne (Liedtke et al. 2017).

Morphological methods
Osteological data were obtained from six specimens of the Serra da Neve population, 18 additional Poyntonophrynus specimens, and five specimens of Mertensophryne (see Suppl. material 1). We performed high-resolution x-ray computed tomography (CTscanning) at the University of Florida's Nanoscale Research Facility. We used a Phoenix v|tome|x M (GE Measurement & Control, Boston, USA) scanner with a 180 kv x-ray tube with a diamond-tungsten target and with the following settings: Voltage = 75-100 kV, Current = 200 mA, 0.2-0.3 second detector capture time, averaging three images per rotation, and voxel resolution of 17-31μm (see Suppl. material 1: Table 1 for details). Raw 2D x-ray data were processed using the datos|x software v. 2.3 with post-processing, analyses (including segmentation), and visualization conducted using VG StudioMax v. 3.1 (Volume Graphics, Heidelberg, Germany). Both tomogram stacks (in TIFF format) and surface models for the CT-scanned specimens are available in MorphoSource (see Suppl. material 1). Measurements and external morphological data followed the standardized protocols presented by Watters et al. (2016). Measurements were taken with an electronic caliper accurate to 0.01 mm, rounded to 0.1 mm. The following measurements were taken of adults: SVL snout-vent length; HW head width, taken at the angle of the jaw; HL head length, from the posterior of the jaws to the tip of the snout; ED eye diameter, horizontally from the anterior to posterior corner of the eye; IND internarial distance, shortest distance between the inner margins of the nostrils; EN eye-nostril distance, from the anterior corner of the eye to the posterior margin of the nostril; TD tympanum diameter, greatest horizontal width of the tympanum; SL snout length, distance from the tip of the snout to the anterior corner of the eye; NS snout-nostril length, distance from the center of the external nares to the tip of the snout; IOD interorbital distance, the shortest distance between the anterior corners of the orbits; UEW upper eyelid width, greatest width of the upper eyelid margins, measured perpendicular to the anterior-posterior axis; FLL forearm length, from the flexed elbow to the base of the outer palmar tubercle; HAL hand length, from the base of the outer palmar tubercle to the tip of finger IV; Fin4L finger IV length, from the proximal edge of the palmar tubercle to the tip of finger IV; TL tibiofibula length, distance from the outer surface of the flexed knee to the heel/tibiotarsal inflection; THL thigh length, distance from the vent to the knee; FL foot length, from the base of the inner metatarsal tubercle to the tip of toe IV; Toe4L Toe IV length, from the metatarsal tubercle to the tip of toe IV.

Results
Molecular phylogenetic and morphological analyses suggest that the pygmy toads collected from the Serra da Neve represent an undescribed species of Poyntonophrynus. The phylogenetic analysis reveals that the Serra da Neve population is nested within the genus Poyntonophrynus and is most closely related to P. fenoulheti (Figure 3) with weak bootstrap support (73%). As expected, since we used data from Liedtke et al. (2017), the eastern African species P. lughensis is more closely related to Mertensophryne than to its putative congeners. Within the well-supported clade (bootstrap support 80%) of other species of Poyntonophrynus, P. grandisonae is sister to remaining congeners. However, as our data do not include overlapping sequences between P. grandisonae and P. hoeschi, our result should be taken with caution. Morphologically, the Serra da Neve taxon is typical of Poyntonophrynus by being a small toad, lacking a tarsal fold, and having indistinct, flattened parotoid glands. However, this population also exhibits distinct morphological characters that clearly distinguish it from all described species of the genus, most notably the lack of tympanum and a combination of several morphological and coloration characters (Table 2). Based on these differences, this population is described here as a new species. Diagnosis. Poyntonophrynus pachnodes sp. n. is a small-bodied bufonid that lacks tarsal folds, a character that distinguishes it from bufonids in Angola except Mertensophryne and Poyntonophrynus. It differs from all Mertensophryne in having inconspicuous parotoid glands, compared to pronounced parotoid glands that form a shelf in the scapular region of Mertensophryne, and in lacking reduction of the phalanges (Grandison, 1981). The newly described species differs from all other members of the genus Poyntonophrynus in lacking a tympanum and columella.
Description of the holotype. Small (SVL 31.4 mm), robust, stout and gravid female, with moderately robust limbs (Figure 4-5; All measurements in Table 3); head triangular, wider than long; snout projecting slightly beyond upper jaw; rostral tip straight in dorsal, ventral and lateral views; eyes projecting laterally just beyond eyelids and approximately flush with margins of head in dorsal view; eye projecting about 50% above dorsal mar- Table 2. Comparison between the Poyntonophrynus pachnodes sp. n. with its congeners. Data based on our observations of freshly collected material, original descriptions, and the revisionary works of Poynton and Broadley (1988), Channing (2001), and du Carruthers (2009, 2017  gin of head in lateral view; interorbital distance 1.1 times eye diameter; pupil large and ellipsoid in life and preservative; loreal region concave; naris small, triangular, directed dorsolaterally; canthus rostralis short, eye diameter 1.2 times eye-narial distance; eye diameter 1.6 times naris to rostral tip; internarial region flat with rounded lateral margins; interorbital distance approximately 1.7 times internarial distance; tympanum and middle ear structures (tympanum, columella) absent. Marginal and vomerine teeth absent. Skin of venter with evenly scattered miniscule asperities; skin of gular region smooth; skin of limbs, dorsal and dorsolateral surface of head and body with scattered tubercles, being larger on dorsum; inconspicuous and flattened parotoid glands, elliptical, and weakly elevated, placed dorsolaterally and extending from posterior corner of mouth to level of axilla. Limbs and digits well-developed; digits of both manus and pes stout; tarsal fold not present; relative length of fingers: III > I > IV = II; finger tips not expanded, but with rounded tips; fingers with rounded, prominent double subarticular tubercles; two palmar  tubercles distinct and widely separated from one another, one at ventromedial surface of first finger and other at proximal plantar surface, latter being about 4 times larger than first; webbing between manual digits absent; relative length of toes IV > III > II > I = V; toe tips slightly expanded; toes with prominent, single, and subarticular tubercles; webbing between toes vestigial, not reaching first joint of first phalanx; prominent and globular inner metatarsal tubercle, length 50% of first toe length; tarsal tubercle prominent and projecting, near medial edge and positioned at distal fourth of tarsus.
Coloration. In life, dorsal ground color dark brown with coppery to brown mottling and dark brown blotches especially in anterior regions ( Figure 6); whitish chevron extending between eyes (scapular patch), directed posteriorly (faded but visible in preservative); iris dark green with dark brown pupil (dark grey and pale gray in preservative, respectively); snout similar in color and pattern to dorsum; dorsal surface of forelimbs and fingers III and IV whitish with dark brown blotches; dorsal surface of fingers I and II white; posterior to head, distinctive white blotch at midbody; dorsum and lateral surface dark brown speckled with coppery markings, becoming faint towards venter; dorsal surface of hind limbs (thighs and crus) greyish white with distinctive dark brown markings; three dark blotches on thigh, crus and feet touching when legs flexed; base color of dorsal foot grayish, with scattered dark brown blotches, extending across toes; region surrounding cloaca cream colored. Lateralmost margin of upper jaw white interrupted by brown markings posterior and anterior to eye; throat immaculate white; ventral surface of forearm and arm whitish; ventral surface of hand Variation. The coloration of the remaining paratypes do not differ in any important details from those of the holotype. Measurements of the remaining type series in Table 3.
Osteology. Based on CT scan of UF 184184 (holotype; gravid female; Figure 7). The skull is wider than long, incompletely ossified, and lacks ornamentation on the dermal roofing bones. The jaw joint is anterior to the otic region. The parotic plate is incompletely ossified but synostosed to the frontoparietal. The premaxillae lack teeth, and have both a robust pars dentalis and a robust alary process that is taller than wide and widely separated from the nasals. The maxillae lack teeth and are weakly concave (with apex directed labially). The quadratojugals are thin and elongate with a broad articulation with the maxillae and reaching anterior to the articulation of the maxilla and pterygoid. The pterygoids are slender, with a long medially curved anterior ramus with a broad articulation with the adjacent maxilla, a short posterior ramus approaching, but not articulating with, the cartilages of the jaw joint, and a short medial ramus approaching but not articulating with the prootic. Vomers are large and plate-like, lacking teeth. The neopalatine is a thin flat rod, not articulating with adjacent bones. The curved and triradiate septomaxillae are present at the anterior margin of the nasal capsule. The prominent sphenethmoid is coosified across the midline and visible in dorsal view be-tween the nasals and frontoparietals. The parasphenoid narrows anteriorly and exhibits a small bifurcation at its rostral extent. The squamosals are greatly reduced with only the dorsalmost otic region and a small reduced zygomatic ramus present. The prootic is poorly ossified and has a poorly defined fenestra ovalis. Neither a bony operculum nor a columella is present. The posteromedial processes of the hyoid are ossified and slender, and expanded weakly at their articulation with the cartilaginous hyoid plate.
There are eight distinct, procoelous, non-imbricating presacral vertebrae that are not synostosed. The atlas lacks transverse processes and has widely separated cotyles. The sacrum is procoelous with laterally expanded transverse processes. No sesamoid is observed at the sacroiliac joint. The urostyle is long and thin with a weakly developed dorsal ridge.
The pectoral girdle is fermisternal with widely spaced and slender coracoids. The clavicles are slender, nearly reaching one another. The scapulae are stout, directed laterally but strongly curving dorsally at their lateral extent. Neither an ossified sternum nor omosternum is present.
The pelvic girdle comprises the ilium, pubis, and ischium, which are not synostosed to one another. The acetabulum is incompletely ossified. The shaft of the ilium is long and slender, lacking a dorsal crest, and with a small prominent dorsal protuberance. The synostosed ischia form a broad posteriorly directed plate.
The radioulna is shorter than the humerus. The radiale and ulnare are large and subequal in size, though the other carpals are difficult to distinguish as they are incompletely ossified. The phalangeal formula for the manus is 2-2-3-3, and there is both a single ossified prepollex and a small palmar sesamoid. The tips of the terminal manual phalanges are weakly expanded into small knobs. The tibiofibula is slightly shorter than the femur. There are two small distal tarsals. The phalangeal formula for the pes is 2-2-3-4-3 and there is a single small ossified prehallux and a plantar sesamoid. The tips of the terminal pedal phalanges are weakly expanded as in the fingers.
Distribution and ecology. The species is currently only known from the Serra da Neve Inselberg (Figs 2,8) in northern Namibe Province. Specimens were found on moist soil under rocks and leaf-litter at dusk in a semi-open miombo forest area. Grandvaux-Barbosa (1970) considered that Serra da Neve is characterized by a "sparse Miombo," dominated by trees of the genera Julbernardia spp. and Brachystegia spp., and shrubs like Combretum spp. or Annona spp., which were observed at the site. The species was found sympatrically with the frogs Sclerophrys gutturalis and Tomopterna tuberculosa, the lizards Agama schacki, Trachylepis sulcata, Chondrodactylus pulitzerae, Hemidactylus benguellensis, Pachydactylus angolensis, and Heliobolus lugubris, and the snake Hemirhagerrhis viperina.
Etymology. The specific name pachnodes (Gr.) means "frosty" and is used as an adjective (Brown 1954). This is a reference to both the cool climate at the higher elevation where this species occurs (1488 m) and that Serra da Neve (Port.) translates to "mountain of snow." We suggest "Serra da Neve Pygmy Toad" and "Sapo Pigmeu da Serra da Neve" as the English and Portuguese common names respectively.

Discussion
Despite several decades of interest in African pygmy toads (e.g., Poyton 1964, Tandy 1972, Poynton and Haacke 1993, there has been little attention paid to the systematics of the species now referred to as Poyntonophrynus. Until recently, there has been no serious attempt to evaluate the monophyly of this genus. The molecular phylogenetic analysis of Liedtke et al. (2017) demonstrates that P. lughensis is more closely allied to Mertensophryne, and if true then this species should be treated as part of that genus. However, as the sample used in that analysis is not associated with a voucher specimen to confirm the identification, we refrain from taxonomic changes. It still remains uncertain whether B. parkeri, which Tandy (1972, Tandy andKeith 1972) included in the 'vertebralis group,' should be included in Poyntonophrynus; Tandy (1972) provided no specific arguments regarding this assignment. While we have contributed new molecular genetic data for P. grandisonae, we still lack phylogenetic information for several species. In lieu of new genetic resources for molecular phylogenetic analyses, a thorough systematic revision based on morphological data, including osteology, is needed to evaluate the monophyly of Poyntonophrynus and the relationships among its species.
Unlike most African anuran taxa, the species diversity of Poyntonophrynus is concentrated in arid southwestern Africa. Five species (P. damaranus, P. dombensis, P. grandisonae, P. hoeschi, and P. pachnodes sp. n.) are strictly endemic to this region, whereas the others (P. vertebralis, P. beiranus, P. kavangensis, P. fenoulheti, and P. parkeri) are found in arid or mesic habitats extending across southern and eastern Africa. This pattern of species distributions, combined with the fact that P. grandisonae represents the earliest known diverging lineage within the genus, suggests that the origin of this group might have been in southwestern Africa. While a highly unusual pattern for an anuran, this is similar to several squamate taxa for which the arid zones of southwestern Angolan and northwestern Namibia form a center of endemism and diversity (Roll et al. 2017, Marques 2015. Southwestern Angola hosts a large number of endemic species of lizards, including one endemic genus, and ongoing work reveals additional cryptic and endemic diversity among several lizard taxa (Figure 9).
In addition to Poyntonophrynus, the few examples of amphibians found in these xeric areas include Phrynomantis annectens, several species of Sclerophrys, and Tomopterna , Heinicke et al. 2017a. Within Namibe Province, there are 91 documented species of reptiles, representing one-third of the reptile species known for Angola, but only 15 amphibian species, which represent <13% of the amphibian diversity of the country (Marques 2015). Neighboring Benguela Province has similar species diversity (102 reptiles, 36 amphibians) and contains similar habitats. The herpetofaunal diversity of these provinces mostly comprises taxa found in southern Africa (Marques 2015). Though Namibe Province is generally well explored, little is known of the diversity of its inselbergs. The biodiversity of Serra da Neve remains largely undocumented with only two expeditions to it in the last few years (Barker et al. 2015, this study), according to available literature. Other inselbergs and highlands found along the Angolan escarpment, including Mount Moco (Huambo Province) and the highlands of Gabela-Seles and the Kumbira Forest (Kwanza Sul Province), are known to harbor a significant number of endemics and relict populations of vari-ous taxa (Marx 1956, Hall 1960, Hall and Moreau 1970, Huntley 1974, Sekercioglu and Riley 2005, Maiato 2009, Mills 2010, Mills et al. 2011, Cáceres et al. 2015, Carleton et al. 2015, Gonçalves and Goyder 2016, Svensson et al. 2017. While the biogeographic affinities of these areas are not completely understood, there is a general pattern of relictual distributions for taxa otherwise found in central or eastern Africa. Yet due to its southern geographic position, surrounding arid habitats, and the limited existing data on occurring taxa, it appears that the affinities of the flora and fauna of Serra da Neve have more similarities to taxa found typically in southern Africa, as for example representatives of the gekkonid genera Rhoptropus, Chondrodactylus and Pachydactylus, or southwestern African endemics including Hemirhagerrhis viperina and Trachylepis laevis; this new species of Poyntonophrynus provides an additional example. We are, however, still far from understanding the biogeographic affinities of Serra da Neve. The osteology of both Poyntonophrynus and its sister taxon Mertensophryne are poorly documented in comparison to other African bufonid taxa. The skeleton of P. pachnodes exhibits features characterized by Grandison (1981) as common in the vertebralis group, though she was not explicit about the species she included in that group. Those features include the reduction or loss of the shaft of the squamosal and an elongate quadratojugal that attains the articulation between the maxilla and the pterygoid at its anterior extent (Poynton 1991, Graybeal andCannatella 1995). Similar to the condition documented by Grandison (1981) for P. vertebralis, the neopalatines are reduced in P. pachnodes ( Figure 7D), in contrast to its well-developed state in Mertensophryne (Grandison 1981, Poynton 1991. All of the species that are currently recognized as Mertensophryne lack external ears (Tandy 1972, Tandy and Keith 1972, Frost et al. 2006), though it is not clear whether all of these species might also lack other structures such as the columella (Tihen 1960). Poyntonophrynus pachnodes does not exhibit reduction in the number of presacral vertebrae, a character which is present at least some Mertensophryne (e.g. Figure 10; Tihen 1960, Grandison 1978, nor does it exhibit any of the ornamentation found in the dorsal skull bones, as in Mertensophryne anotis (Poynton 1991). The newly described species represents the first known case of complete loss of the Tympanic Middle Ear (TME) within the genus Poyntonophrynus (Figure 10), though all of the species in the sister-genus Mertensophryne lack the TME (Tandy and Keith 1972). This condition is generally rare among amniotes, but it is fairly common among anurans, including the Bufonidae (Pereyra et al. 2016). In some cases, the TME is not completely lost, and some portions of the middle ear may remain. The reduction of TME structures in anurans follows a consistent pattern (Pereyra et al. 2016): the absence of more medial structures (e.g., columella) is associated with the absence of lateral structures such as the tympanic annulus and membrane, but the absence of the lateral structures are not necessarily associated with the reduction or loss of the columella. The evolutionary loss of TME and the alternative auditory mechanisms that might be used by these taxa have received substantial recent attention (Boistel et al. 2011, Thomas et al. 2014, Pereyra et al. 2016, Goutte et al. 2017  . Given the loss of the TME in Mertensophryne and at least once species of Poyntonophrynus, this clade may provide an opportunity for further examination of the causes of loss and reduction of the TME and other correlated features (e.g., Womack et al. 2018).