Maximum likelihood (ML) trees of studied Achaeta species based on the ITS region (A) and CO1 (B) gene. Bootstrap values greater than 50 are shown at the nodes. Sequences from new species described here appear in bold. A ML tree of the ITS region based on 736 nucleotide positions using the K2+G substitution model B ML tree of the CO1 gene based on 543 nucleotide positions using the GTR+G+I substitution model. Scale bars: 0.1 substitutions per nucleotide position.