Corresponding author: Thomas Kaltenbach (
Academic editor: B. Price
One new species of
Kaltenbach T, Gattolliat J-L (2017) New species of
The genus
The specimens were collected by Kate Baker (King’s College London, UK) in the Ulu Temburong National Park in Brunei Darussalam with surber and kick-sampling methods (
The specimens were preserved in 70%–96% ethanol. The dissection of larvae was done in Cellosolve (2-Ethoxyethanol) with subsequent mounting on slides with Euparal liquid, using an Olympus SZX7 stereomicroscope.
The DNA of part of the specimens was extracted using non-destructive methods allowing subsequent morphological analysis (see
Sequenced specimens.
Species | Locality | Specimen catalog # | GenBank # ( |
GenSeq Nomenclature |
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Brunei | GBIFCH 00280816 |
|
genseq-1 |
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Brunei | GBIFCH 00280817 |
|
genseq-2 |
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Flores | GBIFCH 00280818 |
|
genseq-4 |
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Flores | GBIFCH 00280819 |
|
genseq-4 |
|
Sumbawa | GBIFCH 00280820 |
|
genseq-4 |
|
Sumbawa | GBIFCH 00280821 |
|
genseq-4 |
Timor | GBIFCH 00280822 |
|
genseq-1 |
Drawings were made using an Olympus BX43 microscope. Photographs of larvae were taken using a Canon EOS 6D camera and the Visionary Digital Passport imaging system (
The distribution map was generated with the program Simple Mapper (
The taxonomic descriptions presented herein were generated with a DELTA (
Habitus, larvae:
Both
Refers to the noticeable spatulate submarginal dorsal setae of the labrum.
Only known from Brunei, but presence in other regions of Borneo such as Sarawak and Sabah (Malaysia) is possible as their fauna remains poorly known.
The specimens were collected in lowland tropical rainforest at an altitude of about 100 m a.s.l., directly at the confluence of small tributaries with large rivers (Belalong, Temburong) as well as in upstream pools of these tributaries (Fig.
Five larvae (in alcohol, GBIFCH 00515216). Brunei, Temburong District, Ulu Temburong National Park, near Kuala Belalong Field Studies Centre, tributary to Temburong river,
Both
After the type locality, the island of Timor (Indonesia).
Indonesia: Timor.
The specimen was collected at an altitude of 1580 m a.s.l.
In addition to the two new species found in Brunei and Timor, the occurrence of
General aspects of
Distribution of
GPS coordinates of locations of examined specimens.
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Sumbawa |
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Flores |
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Timor |
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Brunei |
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Genetic distances (
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Flores | Sumbawa | Timor | |
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0.01 | ||
Sumbawa | |||
0.15 | 0.14 | ||
Timor | |||
0.20 | 0.20 | 0.22 | |
Brunei |
The generic description was given by
Based on the additional two new species, the following adaptations to the generic diagnosis are proposed: the maxillary palp differs between the species, always with three segments and longer than galea-lacinia, but may be very long and slender (
Taking into account the taxa described herein, the genus
Contrary to mouthparts, legs and abdomen, which are showing important specific differences within
From
The presently known distribution of the genus
We sincerely thank Kate Baker (King’s College London, UK) for the allocation of precious mayfly material to the Museum of Zoology Lausanne (MZL), for the photos of larval habitats, and for corrections of the English language. This material was collected during ecological studies in Brunei Darussalam, funded by Natural Environment Research council (NERC), and was in collaboration with Universiti Brunei Darussalam. We are also deeply grateful to Michael Balke (Zoologische Staatssammlung München,