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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Sample DNA and RNA extraction
    • Library construction and sequencing
    • Genome size estimation and assembly
    • Genome annotation
    • Phylogenetic tree construction and divergence time evaluation
    • Estimation of relative evolutionary rates
    • Estimation of gene family expansion and contraction
    • Detection of positive selection
  • Results
    • Assembly and characterization of the roughskin sculpin genome
    • Comparative genomics and phylogenetic analysis
    • Genetic alterations correlated with diadromous migration adaptations
  • Discussion
    • Characteristics of genomic sequences in the roughskin sculpin
    • Genetic origin of the diadroumous lifestyle in roughskin sculpin
    • Genomic signatures associated with the diadromous adaptations in the roughskin sculpin
  • Conclusions
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Use of AI
    • Funding
    • Author contributions
    • Data availability
  • References
  • Supplementary materials
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