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  • ContentsContents
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Study sites
    • Environmental variables
    • Identification and preparation of specimens
    • DNA extraction, amplification, and sequencing
    • Sequence editing
    • Bayesian phylogenetic reconstruction
    • Molecular species delimitation using GMYC
    • Species abundances and environmental variables
  • Results
    • Dataset
    • Species delimitation and life stage association
      • GMYC Analysis
    • Larval abundance and distributional patterns
  • Discussion
    • Sampling
    • Genetic markers
    • Rhyacophila co-occurrence
    • Larval abundance and environmental gradients
  • Outlook and conclusions
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Use of AI
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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