Corresponding author: Nicolas Lavesque (
Academic editor: Greg Rouse
A new species of
Lavesque N, Daffe G, Bonifácio P, Hutchings P (2017) A new species of the
In Arcachon Bay, blood worms of the genus
The family
According to
Both morphological and molecular analyses confirm the existence of an undescribed species of
Specimens examined in this study were collected in Arcachon Bay (Fig.
Sampling sites of
Selected parapodia along the body were removed from the holotype
The studied material is deposited at the
Sub-samples for DNA analysis were removed from live specimens, placed in ethanol 96% and frozen at –20°C. Extraction of DNA was done with QIAamp DNA Micro Kit (QIAGEN) following protocol supplied by the manufacturers. Approximately 400 bp of 16S and 700 bp of
The
Overlapping sequence (forward and reverse) fragments were merged into consensus sequences and aligned using Clustal Omega. For
List of terminal taxa used in molecular analysis, GenBank accession numbers, genes analysed, and voucher specimen catalog numbers.
|
|
|
|
|
|
||
|
|
||
|
|
||
|
|
||
|
|||
|
|
||
|
|
||
|
|
||
|
|
||
|
|
||
|
16S | ||
|
|
||
|
|
||
|
|
||
|
|
||
|
|
||
|
|
||
|
|
||
|
|
||
|
16S | ||
|
|
||
|
16S | ||
|
|
||
|
|
W.49048 |
1 Sequences from
2 Sequences from
3 Sequences from
Pair-wise Kimura 2-parameter (
Holotype:
(based on holotype and paratypes). Live specimens iridescent, dark red with lighter spots, prostomium appendages and parapodia green olive, end of prostomial appendages whitish, branchial filaments red and iridescent. Recently fixed specimens olive-green to brown with lighter spots, prostomium appendages and parapodia pinkish. Preserved holotype with brown mottled pigmentation anteriorly increases in intensity towards prostomium, antennae, and palps whitish.
Body long, with same width throughout, slightly tapering at anterior and posterior ends. Prostomium shorter than anterior ring of peristomium, as wide as peristomium, bilobed with buccal lips separated by deep ventral and dorsal notch with each lobe rounded with base of them strongly pigmented (Fig.
Pre-chaetal neuropodia lobe inconspicuous. Post-chaetal neuropodial lobe conical in the 2–3 first chaetigers, elongate rectangular from chaetiger 4, gradually thereafter becomes wider and rounded; longer than chaetal lobe in anterior chaetigers, shorter in median and posterior chaetigers (Figs
Branchiae pectinate (Fig.
Chaetae arranged in two bundles: supra-acicular and sub-acicular, separated by a row of aciculae (Fig.
SEM images of
Pygidium with only one pair of long pygidial cirri on ventral margin (approximately as long as last 15 segments), anus slightly crenulated with 12 small indentations (Fig.
Paratypes with branchiae starting from chaetigers 26 (
This species is named after Victor Lavesque, first and second authors’ son.
NE Atlantic, France, Arcachon Bay.
Intertidal on mudflats, under or close to oyster reefs or abandoned oyster farms, 5 to 60 cm depth. Few specimens were found in galleries into old piece of driftwood.
As the identification of
Maximum likelihood tree based on cytochrome oxidase I (
Three species are known to occur in Arcachon bay:
Alternatively,
To conclude, we suggest that all records of
1 | Compound spinigers only |
|
– | Both compound falcigers and spinigers |
|
2 | Branchiae limited to anterior chaetigers |
|
– | Branchiae present over most of the body |
|
3 | Branchiae from chaetigers 13 to 27, absence of anodont, asymmetrical pectinate chaetae with 2–4 teeth, subacicular hooks present |
|
– | Branchiae from chaetigers 26 to 34, presence of anodont, asymmetrical pectinate chaetae with 2–4 teeth, no subacicular hooks |
|
4 | Branchiae with up to 2 filaments |
|
– | Branchiae with 6 or more filaments |
|
5 | Compound spinigers limited to anterior 1/3 or less |
|
– | Compound spinigers along nearly entire body |
|
We would like to thank Gaby Binois and Benoit Planella, the best bait fishers in Arcachon Bay for providing us with many complete specimens and for indicating the different locations in the bay where they collect the species. Many thanks to Benoit Gouillieux who provided us with some specimens. Sue Lindsay mounted the parapodia for the SEM and took the images. Finally, we also thank Joana Zanol, Greg Rouse, and an anonymous reviewer to provide helpful comments on the submitted manuscript.