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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Sample collection and preparation
    • Muscle histological assay and muscle thickness measurement
    • RNA extraction, cDNA library construction, and sequencing
    • Data processing and bioinformatics analysis
    • Analysis of DEGs
    • Analysis of DAS
    • Data validation by real-time PCR
    • Statistical analysis
  • Results
    • Muscle structure and thickness in eyed side and blind side of C. semilaevis
    • Summary statistics of transcriptome sequencing
    • Identification and comparative analysis of the DEGs
    • Functional enrichment of DEGs
    • Alternative splicing analysis
    • Validation of the DEGs by qRT-PCR
  • Discussion
    • Muscle morphology of eyed-side muscle and blind-side muscle in C. semilaevis
    • Transcriptome analysis of ESM and blind-side muscle in C. semilaevis
  • Conclusions
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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