Corresponding author: Xu Zhang (
Academic editor: V. Pesic
A new species of the water mite genus
Ding J-H, Sun J-L, Zhang X (2017) A new species of the water mite genus
Species identification based on the 658bp sequence of mitochondrial cytochrome oxidase I gene (
During checking of a recent collection of water mites, three species (
Water mites were collected by hand netting and preserved in absolute ethanol in 1.5ml centrifuge tubes. The centrifuge tubes were transported to the laboratory and stored at -20°C. The information of the samples used in this study is given in Table
Samples examined in this study.
Species | Sex | BOLD process ID | GenBank accession numbers |
---|---|---|---|
|
Male |
|
|
|
Male |
|
|
|
Female |
|
|
|
Female |
|
|
|
Female |
|
|
|
Male |
|
|
|
Male |
|
|
|
Male |
|
|
|
Male |
|
|
Female |
|
|
For molecular examination, each mite was transferred in individual 1.5ml tubes, and washed several times with sterile deionized water. Non-destructive DNA extraction was done on the whole mite. The genomic DNA was extracted by using the DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions. Then, the mites were fixed in absolute ethanol and stored at -20°C for morphological analysis.
The standard
All the sequence data were analysed by using MEGA (ver. 6;
For morphological examination, the mite was dissected as described elsewhere (e.g.
The type specimens are deposited in School of Life Sciences, Huaibei Normal University, China. All measurements are given in µm.
Holotype: Female, Anhui Province, Fuxi village, Monkey Valley scenic area, an unnamed stream (
Integument fine spinules arranged in hexagonal pattern;
The species is named after the village where it was collected.
Due to the shape of the integument, P- II with a very long ventrodistal projection, excretory pore surrounded by sclerotized ring, and third to fifth segments of leg I-IV with plumose setae, the new species is similar to
China (Anhui Province).
2 females, Guizhou Province, Fanjingshan National Nature Reserve, an unnamed stream (
Due to the shape of integument,
Although there are many differences between the male of
Burma, China (Anhui, Guizhou, Taiwan), Turkey, Iran.
The ten nucleotide sequences of 658 bp obtained belong to four species (
Phylogenetic tree based on barcode region of
Genetic distances (K2P) for barcode region of CO I between the species analysed in this study were shown in Table
Genetic distances (K2P) for barcode region of CO I among the
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
---|---|---|---|---|---|---|---|---|
1 |
||||||||
2 |
0.012 | |||||||
3 |
0.009 | 0.003 | ||||||
4 |
0.009 | 0.003 | 0.000 | |||||
5 |
0.168 | 0.171 | 0.167 | 0.167 | ||||
6 |
0.170 | 0.169 | 0.169 | 0.169 | 0.002 | |||
7 |
0.166 | 0.168 | 0.164 | 0.164 | 0.006 | 0.008 | ||
8 |
0.158 | 0.161 | 0.157 | 0.157 | 0.009 | 0.011 | 0.009 | |
9 |
0.364 | 0.366 | 0.366 | 0.366 | 0.324 | 0.322 | 0.322 | 0.322 |
In this study, the female of
The morphological characters of the male
In recent years, the research of molecular identification and phylogeny have been reported in many genera of water mites, such as
According to the previous research and our study, the intra- and interspecific divergences of water mites were variable among different groups. For example, the intraspecific divergence value was 0.2% in
The instable divergences among the different species of water mites may be ascribed to limited data and researches on DNA barcoding for water mites. More molecular researches on water mites are required to solve this problem.
We are very grateful to Dr. Jian-Jun Guo (Guizhou University, China) for the specimen collection and Dr. Dao-Chao Jin (Guizhou University, China) for his help in many ways. Furthermore, we are grateful to Dr. Ling-Ying Shuai (Huaibei Normary University, China) for providing helpful comments and suggestions on this manuscript. This study was supported by The National Science Foundation of China (No. 31301895).