Maximum Likelihood analysis of selected species of the genus Agonopterix: A. pallorella-group, A. multiplicella and species near A. pseudoferulae. In addition to the selection used for the neighbour-joining tree, the following Agonopterix species were included: A. angelicella (Hübner, [1813]) (TLMF Lep 06308, BOLD:AAE3381); A. argillacea (Walsingham, 1881) (BIOUG05263-B04, BOLD:ACF4423); A. assimilella (Treitschke, 1832) (TLMF Lep 03008, BOLD:AAJ7526); A. walsinghamella (Busck, 1902) (BIOUG07080-A07, BOLD:ACF9151). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura-Nei model. The tree with the highest log likelihood (-4818.52) is shown. Initial trees for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. The analysis involved 54 nucleotide sequences from selected species of Agonopterix and Depressaria. Only the Agonopterix part of the tree is shown here. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 657 positions in the final dataset. Evolutionary analyses were conducted in MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets (Kumar, Stecher and Tamura 2015). The result is shown in radiation graphic, because in this view the evolutionary aspect is visualized better than in rectangular tree.

 
 
  Part of: Buchner P, Corley M, Junnilainen J (2017) Three new species and one new subspecies of Depressariinae (Lepidoptera) from Europe. ZooKeys 684: 119-154. https://doi.org/10.3897/zookeys.684.13383