Neighbour-joining tree was constructed based on mtDNA 16S rRNA and COI gene. The tree showing genetic distances among Limnichthys species. Bootstrap values based on 1000 replicates were showed 100%, the values are omitted. The red box indicates Limnichthys koreanus sp. nov.; blue box indicates L. fasciatus from Southeastern Australia; green boxe indicates L. cf. nitidus from Japan; yellow box indicates L. orientalis from Japan; Trichonotidae species was used as outgroup.