Differences in the acoustic parameters of E. currulao sp. nov. compared to other species of Epipedobates found in Colombia A the relative contribution of each acoustic variable to the first five principal components obtained in the Principal Component Analysis. The absolute value of each contribution is represented according to the size of the circle, while blue and red colors show positive and negative contributions, respectively B a Linear Discriminant Analysis using PCs as input indicates E. currulao can be differentiated from other species based on their advertisement calls, particularly regarding call duration and number of pulses (PC1 loads positively on LD1). Dots indicate the individuals, color indicates the species, and ellipses indicate the 95% confidence intervals for LDA data points.