Maximum Likelihood (ML) tree of concatenated COI and 16SrDNA haplotypes of Monacha cantiana. New COI and 16SrDNA sequences of M. cantiana (Table 1, Suppl. material 5) were compared with concatenated COI and 16SrDNA sequences of M. cantiana s.l. and M. cartusiana obtained from GenBank (Suppl. materials 1, 2, 5). Length of sequences was 1444 positions (615 of COI + 829 of 16SrDNA). The Bayesian Information Criterion (BIC) specified T92+G+I the best nucleotide substitution model in MEGA7, or HKY+F+G4 for COI and TIM2+F+G4 for 16SrDNA partition in IQ-Tree, RAxML and MrBayes. Numbers next to main branches indicate (left to right): bootstrap supports above 50% calculated by NJ-MEGA7 (Saitou and Nei 1987), ML-MEGA7 (Kumar et al. 2016), IQ-Tree (Trifinopoulos et al. 2016), RAxML (Stamatakis 2014), and posterior probabilities by BI (Ronquist et al. 2012). The tree was rooted with Trochulus hispidus concatenated sequences obtained from GenBank (Suppl. material 5).

 
 
  Part of: Pieńkowska JR, Manganelli G, Proćków M, Barbato D, Sosnowska K, Giusti F, Lesicki A (2024) Next step in Monacha cantiana (Montagu, 1803) phylogeography: northern French and Dutch populations (Eupulmonata, Stylommatophora, Hygromiidae). ZooKeys 1198: 55-86. https://doi.org/10.3897/zookeys.1198.119738