Maximum Likelihood (ML) tree of concatenated H3 and ITS2 (flanked with 5.8S and 28SrDNA) haplotypes of Monacha cantiana. New H3 and ITS2 sequences of M. cantiana (Table 1) were compared with concatenated H3 and ITS2 sequences of M. cantiana s.l. obtained from GenBank (Suppl. materials 3, 4). Length of sequences was 1054 positions (279 of H3 + 775 of ITS2). The Bayesian Information Criterion (BIC) specified T92+G+I the best nucleotide substitution model in MEGA7, or K2P+I for H3 and K3P+I for ITS2 partition in IQ-Tree, RAxML, and MrBayes. Numbers next to main branches indicate (left to right): bootstrap supports above 50% calculated by NJ-MEGA7 (Saitou and Nei 1987), ML-MEGA7 (Kumar et al. 2016), IQ-Tree (Trifinopoulos et al. 2016), RAxML (Stamatakis 2014) and posterior probabilities by BI (Ronquist et al. 2012). The tree was rooted with Trochulus hispidus concatenated sequences obtained from GenBank (Suppl. material 6).

 
 
  Part of: Pieńkowska JR, Manganelli G, Proćków M, Barbato D, Sosnowska K, Giusti F, Lesicki A (2024) Next step in Monacha cantiana (Montagu, 1803) phylogeography: northern French and Dutch populations (Eupulmonata, Stylommatophora, Hygromiidae). ZooKeys 1198: 55-86. https://doi.org/10.3897/zookeys.1198.119738