Microtubules in hindgut cells of early-stage marsupial mancae A overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488). Image depicts a transverse section across the anterior part of manca. Arrowheads point to microtubules in dorsal tergites B overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by frame B in image A C overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts hindgut epithelium at the transition between the anterior chamber and the papillate region. Arrowheads point to cells containing distinct apico-basal bundles of microtubules D overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts hindgut epithelium from the anterior part of the papillate region E overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts hindgut epithelium from the posterior part of the papillate region F electron micrograph of equivalent area as denoted by frame F in image C showing the cell in the ventral hindgut epithelium. Arrows point to small bundles of microtubules in the cytosol. Abbreviations: DG – digestive glands; HE – hindgut epithelium; HG – hindgut; HL – hindgut lumen; M – muscles; T – typhlosole; TC – typhlosole channels; VNC – ventral nerve cord.