Microtubules in hindgut cells in the rectum in adult P. scaber A overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts cross-section across the rectum B overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by frame B in image A C overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts cross-section across the rectum near the anal opening D overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by frame D in image C. Abbreviations: AN – anal opening; HC – hemocoel; HE – hindgut epithelium; HL – hindgut lumen; M – dilator muscles.