Microtubules in hindgut cells in the sphincter region in adult P. scaber A overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts cross-section across the sphincter region B overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by frame B in image A C overlay of epifluorescence images obtained on semithin LR-White section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts cross-section of hindgut epithelium in sphincter region at high magnification. The white dashed lines in figure C outline the contour of hindgut epithelium. Abbreviations: HC – hemocoel; HE – hindgut epithelium; HL – hindgut lumen; M – muscles.