Microtubules in hindgut cells in the posterior part of anterior chamber of adult P. scaber A overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts cross-section across the anterior chamber near the transition to the papillate region B overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by the frame B in image A C overlay of epifluorescence images obtained on semithin LR-White section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts equivalent area as image B. Arrow points to large cell that surround the typhlosole channels and contain abundant microtubule bundles. Arrowheads point to parallel bundles of microtubules oriented perpendicular to the apical surface of typhlosole cells. The white dashed lines outline the contour of hindgut epithelium D overlay of epifluorescence and DIC images obtained on paraffin section showing microtubules in green (AlexaFluor 488) and cell nuclei in blue (DAPI). Image depicts area denoted by the frame D in image A. Abbreviations: HC – hemocoel; HE – hindgut epithelium; HL – hindgut lumen; T – typhlosole; TC – typhlosole channels.