Myobiid mites (Trombidiformes, Myobiidae) of the golden bat Mimon cozumelae from Mexico. Description of the male and tritonymph of Ioanella mimon and new records of Eudusbabekia mimon

Abstract The male and the tritonymph of Ioanella mimon are described for the first time parasitizing to Mimon cozumelae from Yucatan, Mexico. Male of Ioanella mimon is characterized by the presence of legs I with the tibia and tarsus fused forming a small complex devoided of apical claws, legs II–IV with two claws, setae vi at level of anterior end of genital plate, genital plate rounded with an anterior projection, all intercoxal setae short; while the tritonymph is characterized by the presence of legs I unequal; legs II–IV with 2-1-1 claws, and posterior region of dorsal idiosoma with 3 pairs of cylindrical and toothed setae. Additionally, we include new locality and host records for Eudusbabekia mimon which was also found on Mimon cozumelae. Both species were described originally in association with Mimon bennettii at Bartica, Guyana.

Particularly Eudusbabekia mimon Fain, 1973 and Ioanella mimon (Fain, 1973) were recorded parasitizing to Mimon bennettii Gray, 1938 from Bartica, Guyana (Fain 1973). Type material of both species is deposited in the Natural History Museum of London. The objective of this work is to provide the first morphological description of the male and tritonymph of I. mimon, and new host and locality records for both species associated with Mimon cozumelae Goldman, 1914 from Yucatan, Mexico.

Methods
A total of five bats were captured inside two hollowness located at carretera Santa Elena-Loltún Km 56, Yucatán, México (20°17'25.0"N, 89°38'43.3"W, 98 m) (Fig. 1). Bats were captured using mist nest and individually maintained until their posterior revision with a dissecting microscope. The Myobiidae (adults and nymphs) were removed from bats using fine, sharp forces and fixed and preserved in vials with 96% ethanol. The specimens were cleared in lactophenol and mounted in Hoyer's medium. Mites were determined taxonomically. Descriptions and nomenclature for idiosomal setation follows Bochkov et al. (2008). Measures of body and setae are in micrometers and were made on a microscope Zeiss Axioscope 2 plus (Göttingen, Niedersachen, Germany), using the AXIOVISION 4 software; for measures we provide the average, followed by range in parenthesis. Drawings of specimens were made with a phase contrast microscope (Zeiss), equipped with a drawing tube. For the scanning electron microscopy (SEM), the specimens were dehydrated in 100% ethanol and dried to a critical point with liquid carbon dioxide. The dried specimens were mounted on aluminum specimen stubs, coated with a gold palladium alloy, and examined using a scanning electronic microscope Hitachi Stereoscan Model S-2469 N SEM (Hitachi Ltd., Tokyo, Japan). Mites were deposited at Colección del Laboratorio de Acarología, Facultad de Ciencias (LAFC), Universidad Nacional Autónoma de México (UNAM). Host were captured under the permission SGPA/DGVS/08257/13 and deposited at Colección de Mamíferos, Museo de Zoología "Alfonso L. Herrera", Facultad de Ciencias (MZFC), UNAM.
Description. Male (Based on 2 males). Body length 225 (223-228); wide 139 (125-152). Body 1.6 larger than wide. Dorsal idiosoma ( Fig. 2A). With a reduce number of setae. All dorsal setae slightly toothed except setae vi; vi at level of anterior end of genital plate; setae sci cylindrical, and situated close to the genital aperture.  Gnathosoma. Normally developed, with a pair of ventral flat and retrorse processes as in the female (Fain, 1978) but slightly less pronounced.
Remarks. The male described in this study was determined as part of the genus Ioanella by the presence of legs I with the tibia and tarsus fused forming a small complex devoided of apical claws, legs II-IV with two claws, vi and sci thin and short, all intercoxal setae very short and the lacking of f2 (Fain 1978). The tritonymph was characterized by the presence of legs I unequal in shape and legs II-IV with 2-1-1 claws (Fain 1978).
The identification of males and tritonymphs as I. mimon was done correlating the presence of females on the same analyzed bats considering that myobiids exhibit high specificity to their hosts (Fain 1994).
Comparing our male specimens with the female described by Fain (1973), the only differences observed were in relation to femur and genua III due to we reported three setae instead of two and three setae instead of four, respectively.
This work represents the first description of a male of the genus Ioanella, and the second that describes a tritonymph for the genus; previously Fain (1973) described the tritonymph of Ioanella chrotopterus (Fain, 1973).
Eudusbabekia mimon and I. mimon are two species of myobiids recorded originally parasitizing to M. bennettii, in this work both species are referred for the first time in association with M. cozumelae, species formerly included as subspecies of M. bennettii (Ortega and Arita 1997, Villa-Ramírez 1967, Hall 1981, but considered by McCarthy (1987) and Wilson and Reeder (2005), as valid species.
Recent studies suggest that there is no sufficient morphological evidence to maintain M. cozumelae in a specific level (Gregorin et al. 2008;Hoppe and Ditchfield 2015).
On the other hand, Hurtado and Pacheco (2014) suggested that the genus Mimon is not a monophyletic taxon. They proposed to elevate to a genus category the two subgenera (Mimon and Anthorhina) referred by Gardner and Patton (1972). In accordance with Hurtado and Pacheco (2014), the genus Mimon must include to M. bennettii and M. cozumelae, and the genus Gardnerycteris (=Anthorhina) to Gardnerycteris crenulatum (É. Geoffroy, 1803) and Gardnerycteris koepckeae (Gardner and Patton, 1972). In this context, E. mimon and I. mimon will be associated with the bat species of the genus Mimon, while Eudusbabekia anthorhinae Dúsbabek andLukoschus, 1974 andIoanella martae Dúsbabek andLukoschus, 1973 to the species of the genus Gardnerycteris. Considering of degree of specificity of myobiid mites to genera or groups of species of hosts (Fain 1994), the referred association could support the Hurtado and Pacheco´s proposal.