Phylogenetic tree of the partial sequences of the 16S gene of the species of Rhinella. The accessions in the present study (in bold), and of the sequences in GenBank (accession numbers in bracket), using maximum likelihood (ML) method under the TIM2+F+I+G4 model. Numbers at nodes are selbranch support analyses; from left to right: ultrafast bootstrap values, and Shimodaira–Haseglike approximate likelihood ratio test (SH-like aLRT). Three species of Anaxyrus sequences were used as outgroups.