Phylogenetic tree of the genus Achalinus inferred from CO1 gene fragment (681 bp) using Maximum Likelihood. The tree nodes present the supporting values: SH-like approximate likelihood ratio test, Ultrafast Bootstrap Approximation and Bayesian posterior probabilities, respectively (SH, %/UFB, %/BI) (the ones lower than 50 are displayed as “-”). Achalinus ningshanensis is noted in blue and A. hunanensis sp. nov. is noted in red.