Corresponding author: João F. R. Tonini (
Academic editor: F. Andreone
Among Neotropical microhylids, the genus
Tonini JFR, Forlani MC, de Sá RO (2014) A new species of
The diversity of evolutionary lineages with little phenotypic differences (i.e., cryptic species) might be better understood in the light of genetic delimitation of evolutionary units (
Species are segments of population level evolutionary lineages and do not necessarily need to be phenetically distinguishable, diagnosable, monophyletic, intrinsically reproductively isolated, ecologically divergent, or anything else to be considered species, but they only have to be evolving separately from other lineages (
A recent molecular phylogeny (
Herein, we describe a new species of
Specimens and tissues used herein and comparative material are deposited in the following collections: 1) : Coleção de Anfíbios Célio Fernando Baptista Haddad, Departamento de Zoologia, Universidade Estadual Paulista Rio Claro, Rio Claro, São Paulo State, Brazil : Museu Nacional do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro State, Brazil : Museu de Biologia Mello Leitão, Santa Teresa, Espírito Santo State, Brazil : Coleção de Tecidos e DNA da Universidade Federal do Espírito Santo (UFES) : Laboratório de Genética Animal, Vitória, Espírito Santo State, Brazil : Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro State, Brazil
Sample localities of
The following measurements were adapted from (snout-vent length) (hand length; from the base of the thenar tubercle to the tip of the third finger) (hand length from the base of the thenar tubercle to the tip of the fourth finger) (head length; from snout to angle of the jaw) (head width; between the angle of jaws) (eye diameter; between anterior and posterior corner of the eye) (inter-orbital distance; distance between anterior corner of the eyes) (inter-nostril distance) (eye-nostril distance; from the anterior corner of the eye to the posterior margin of nostril) (thigh length; from the center of the cloaca opening to the outer edge of the flexed knee) (tibia length; from the outer edge of the flexed knee to the heel) (forearm length) (foot length; from tibia-tarsal articulation to tip of fourth toe) (diameter of third finger disk) (diameter of fourth toe disk)
The following outgroup were chosen based on published phylogenies including species of
Best partition scheme and substitution models selected using Partition Finder.
Subset | Best Model | Subset partitions | Subset sites |
---|---|---|---|
1 | HKY+I+G | 12S | 1–700 |
2 | HKY+G | 16S, ND2_1 | 701–1044, 1661–2473\3 |
3 | K80+I | BDNF | 1045–1660 |
4 | HKY+G | ND2_2 | 1662–2473\3 |
5 | GTR+G | ND2_3 | 1663–2473\3 |
We applied two approaches of phylogenetic estimation: 1) Maximum Likelihood (ML) and 2) Bayesian inference (BI) using the dataset containing the markers 12S, 16S, ND2, and BDNF. Maximum Likelihood in RAXML v7.2.8 (
The phylogenetic hypotheses generated through ML (
Maximum likelihood tree including 12S, 16S, ND2, and BDNF. Node numbers correspond to bootstrap, values >70 indicate good support. Although
Phylogenetic hypothesis obtained through Bayesian Inference using 12S, 16S, ND2, and BDNF. Node numbers correspond to posterior probabilities, values >0.95 indicate good support. Numbers after underscore symbol correspond to localities present in
Therefore, our results show that the new species clusters within the genus
MZUSP147478, adult male, collected at the Floresta Nacional do Rio Preto (
Males: MZUSP147471–73, MZUSP147475–76, MZUSP147494; female: MZUSP147479 (
A small-sized species of
Body small (SVL = 15.7 mm), slender, slightly ovoid (
Holotype of
Arms slender, lacking tubercles on forearm. Hands not webbed (
Skin smooth, dorsal surfaces of body lacking dermal spines. Throat black and few dermal spines found on chin and snout (
Dorsum dark brown with a few small cream spots and blotches; dorsal surface of limbs dark brown with cream blotches and small spots, particularly on the proximal forelimb; palm of hands marbled brown and pale cream, foot dark brown; belly surface cream, dorsolateral and ventral surfaces with a marbled pale brown and cream pattern; throat dark brown to black. Ventral surface of thighs light brown with a finely reticulated dark pattern over a cream background cream with a few cream spots more evident close to the edges; ventral surfaces of tibia and tarsus finely marbled in light brown with cream, lighter than the dorsal surface. Absence of distinct lines on the body and limbs.
(in mm). SVL 15.7; HDL 3.4; HDL4 2.3; HL 2.7; HW 4.5; ED 1.3; IOD 2.8; IND 1.1; END 1.2; THL 6.5; TBL 6.4; FL 6.9, FAL 3.2; 3FD 0.3; 4TD 0.4.
Measurements data of the type series are given in
Morphometric measurements (mm) of the type series of
Specimen | Type | Sex | SVL | HL | HW | ED | IOD | IND | END | THL | TBL | FL | 3FD | 4TD | FAL | HDL | HDL4 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MZUSP147471 | Paratype | Male | 14.4 | 2.8 | 3.8 | 1.0 | 2.6 | 1.1 | 1.5 | 6.1 | 6.3 | 6.2 | 0.3 | 0.3 | 3.0 | 2.8 | 1.8 |
MZUSP147472 | Paratype | Male | 15.3 | 2.8 | 4.1 | 1.3 | 2.7 | 1.2 | 1.3 | 7.1 | 6.6 | 6.6 | 0.3 | 0.4 | 3.2 | 3.4 | 2.2 |
MZUSP147473 | Paratype | Male | 16.1 | 3.2 | 3.9 | 1.1 | 2.2 | 1.1 | 1.3 | 6.0 | 6.0 | 6.4 | 0.2 | 0.2 | 3.2 | 3.3 | 2.1 |
MZUSP147475 | Paratype | Male | 14.5 | 2.8 | 4.6 | 1.3 | 2.6 | 1.3 | 1.3 | 6.6 | 6.4 | 6.7 | 0.4 | 0.4 | 3.3 | 3.5 | 2.2 |
MZUSP147476 | Paratype | Male | 16.1 | 2.7 | 4.4 | 1.2 | 2.7 | 1.3 | 1.4 | 6.7 | 6.3 | 6.1 | 0.3 | 0.5 | 3.0 | 3.3 | 1.8 |
MZUSP147478 | Holotype | Male | 15.7 | 2.8 | 4.5 | 1.3 | 2.8 | 1.1 | 1.3 | 6.6 | 6.5 | 6.9 | 0.4 | 0.4 | 3.2 | 3.5 | 2.3 |
MZUSP147494 | Paratype | Male | 16.6 | 3.1 | 4.6 | 1.4 | 2.6 | 1.2 | 1.4 | 7.0 | 6.9 | 7.6 | 0.3 | 0.4 | 3.3 | 3.8 | 2.4 |
MZUSP147479 | Paratype | Female | 17.2 | 2.9 | 4.6 | 1.3 | 2.9 | 1.3 | 1.4 | 6.7 | 6.6 | 6.8 | 0.3 | 0.4 | 3.3 | 3.5 | 2.3 |
Abbreviations: SVL = snout-vent length; HDL = hand length; HDL4 = hand length from the base of the thenar tubercle to the tip of the fourth finger; HL = head length; HW = head width; ED = eye diameter; IOD = inter-orbital distance; IND = inter-nostril distance; END = eye-nostril distance; THL = thigh length; TBL = tibia length; FAL = forearm length; FL = foot length; 3FD = diameter of third finger disk; 4TD = diameter of fourth toe disk. For tissues numbers see
The specific epithet
Larissa Gaigher and Dr. Yuri Leite inspecting pitfall traps installed at the type locality of
Genetic distance (p-uncorrected) in the BDNF (upper-right) and in the ND2 (lower-left) among
Species |
|
|
|
|
|
||
---|---|---|---|---|---|---|---|
|
|
0.001 | 0.008 | 0.008 | 0.001 | 0.007 | 0.003 |
|
0.064 |
|
0.008 | 0.008 | 0.001 | 0.007 | 0.003 |
|
0.211 | 0.227 |
|
0.005 | 0.008 | 0.004 | 0.007 |
|
0.182 | 0.198 | 0.108 |
|
0.008 | 0.005 | 0.007 |
0.071 | 0.082 | 0.217 | 0.209 |
|
0.007 | 0.004 | |
|
0.206 | 0.204 | 0.107 | 0.103 | 0.22 |
|
0.007 |
0.098 | 0.104 | 0.253 | 0.237 | 0.083 | 0.236 |
|
Differences between
Species |
|
|
||
---|---|---|---|---|
|
SVL=15.1 (SD 0.6) |
Feet webbing | head length; thickness of limbs, fingers, and toes; dermal spines | Feet webbing; thickness of limbs; dermal spines |
|
mtDNA (ND2: 6.4%, 16S: 1.3%, 12S: 1.8%) nuDNA (BNDF: haplotype sharing) | SVL=16.1 (SD 0.9) |
head and limb length; feet webbing; thickness of limbs; dermal spines | body size; thickness; dermal spines |
mtDNA (ND2: 7.1%, 16S: 0.8%, 12S: 1.1%) nuDNA (BNDF: haplotype sharing) | mtDNA (ND2: 8.2%, 16S: 0.8%, 12S: 1.8%) nuDNA (BNDF: haplotype sharing) | SVL=14 (SD 1.4) |
Feet webbing; thickness of limbs | |
mtDNA (ND2: 9.8%, 16S: 2.2%, 12S: 1.2%) nuDNA (BNDF: no haplotype sharing) | mtDNA (ND2: 10%, 16S: 2.3%, 12S: 2.1%) nuDNA (BNDF: no haplotype sharing) | mtDNA (ND2: 8.3%, 16S: 1.6%, 12S: 2.1%) nuDNA (BNDF: haplotype sharing) | SVL=15.3 (SD 0.7) |
SVL snout-vent length; HL head length; THL tight length; TBL tibia length; SD standard deviation.
Cryptic species have challenged our ability to assess current levels of biodiversity. Anuran taxonomy has used various data sources to describe the species diversity, e.g., advertisement calls, external morphology, osteology, tadpoles, ecology, molecular data, karyotypes (
The new species occupy coastal areas North of Espírito Santo State, a region that is under strong human pressure. Therefore, marine and coastal communities are susceptible to impacts of proposed modifications in the landscape for the exploitation of mineral resources. In this context,
We are thankful to L. Costa, Y. Leite, and the Laboratório de Mastozoologia e Biogeografia team, for field assistance and tissues samples. L. Chagas and staffs of the Floresta Nacional do Rio Preto for valuable help during field surveys. M. T. Rodrigues, R. C. Amaro, C. F. B. Haddad, P. Rocha, M. Napoli, H. Zaher, M. Solé, V. Fagundes, J. L. Gasparini, J. P. Pombal Jr., and P. Passos for providing tissue samples and permission to examine specimens under their care. D. Baêta and A. C. Calijorne for hosting JFRT during visit to the Museu Nacional do Rio de Janeiro. R. Ferreira, J. L. Gasparini, M. Vences, R. A. Pyron, F. Andreone, and two anonymous referees for suggestions on the manuscript. JFRT acknowledge support by the Science without Borders program (CAPES/Brazil), The George Washington University, and award NSF-DEB 1144692 to R. O. de Sá.
Examined material and measurements from males included in the morphological analyses.
|
|
|
|
|
|
|
|
IOD | IND | END | THL | TBL | FL | 3FD | 4TD | FAL | HDL | HDL4 |
|
MNRJ17514 | Aracruz | ES | 14.77 | 2.91 | 4.04 | 1.18 | 2.57 | 1.16 | 1.21 | 6.19 | 6.34 | 6.43 | 0.35 | 0.44 | 2.98 | 3.36 | 2.08 |
|
MNRJ17515 | Aracruz | ES | 13.98 | 2.84 | 4.44 | 1.28 | 2.71 | 1.21 | 1.39 | 6.17 | 6.13 | 6.58 | 0.29 | 0.46 | 3.07 | 3.28 | 1.94 |
|
MNRJ17516 | Aracruz | ES | 14.47 | 2.93 | 3.98 | 1.18 | 2.64 | 1.19 | 1.32 | 6.08 | 5.96 | 6.64 | 0.30 | 0.42 | 3.10 | 3.36 | 2.16 |
|
MNRJ17517 | Aracruz | ES | 14.70 | 3.00 | 4.10 | 1.36 | 2.61 | 1.29 | 1.26 | 6.23 | 6.10 | 6.74 | 0.33 | 0.51 | 3.15 | 3.55 | 2.32 |
|
MNRJ17518 | Aracruz | ES | 16.02 | 3.13 | 4.39 | 1.32 | 2.64 | 1.21 | 1.45 | 6.61 | 6.80 | 7.45 | 0.38 | 0.48 | 3.62 | 3.86 | 2.33 |
|
MNRJ17519 | Aracruz | ES | 15.40 | 2.99 | 3.95 | 1.36 | 2.63 | 1.23 | 1.56 | 6.05 | 6.31 | 6.28 | 0.33 | 0.45 | 3.38 | 3.17 | 2.09 |
|
MNRJ17520 | Aracruz | ES | 14.94 | 2.76 | 3.82 | 1.26 | 2.66 | 1.20 | 1.36 | 5.71 | 6.06 | 5.90 | 0.41 | 0.47 | 2.96 | 3.15 | 1.80 |
|
MNRJ17535 | Aracruz | ES | 15.23 | 2.96 | 3.83 | 0.97 | 2.39 | 1.18 | 1.07 | 6.18 | 5.76 | 5.97 | 0.38 | 0.42 | 2.76 | 3.05 | 2.13 |
|
MNRJ17536 | Aracruz | ES | 15.24 | 2.50 | 3.92 | 1.23 | 2.60 | 1.16 | 1.41 | 5.83 | 5.79 | 6.63 | 0.33 | 0.45 | 2.93 | 3.52 | 2.19 |
|
MNRJ17895 | Aracruz | ES | 15.46 | 2.89 | 4.32 | 1.12 | 2.56 | 1.22 | 1.21 | 6.62 | 6.30 | 6.46 | 0.33 | 0.47 | 3.25 | 3.34 | 2.15 |
|
MNRJ22962 | Reserva Natural Vale | ES | 14.83 | 3.02 | 4.44 | 1.26 | 2.67 | 1.18 | 1.38 | 6.29 | 6.08 | 6.37 | 0.36 | 0.44 | 3.12 | 3.34 | 2.08 |
|
MNRJ22966 | Reserva Natural Vale | ES | 15.05 | 2.99 | 4.41 | 1.37 | 2.71 | 1.19 | 1.43 | 6.71 | 6.20 | 6.25 | 0.36 | 0.52 | 3.18 | 3.15 | 1.72 |
|
MZUSP147468 | ReBio Duas Bocas | ES | 15.88 | 3.05 | 4.40 | 1.27 | 2.87 | 1.25 | 1.41 | 6.84 | 6.53 | 6.92 | 0.43 | 0.43 | 3.06 | 3.44 | 2.14 |
|
MZUSP147469 | ReBio Duas Bocas | ES | 16.23 | 3.02 | 4.61 | 1.05 | 2.99 | 1.31 | 1.68 | 6.80 | 6.71 | 7.05 | 0.48 | 0.48 | 3.28 | 3.29 | 2.30 |
|
MNRJ17480 | Horto Florestal | RJ | 16.20 | 3.40 | 4.40 | 1.10 | 2.70 | 0.90 | 1.40 | 6.50 | 6.50 | 7.30 | 0.30 | 0.40 | 3.30 | 3.60 | 2.50 |
|
MNRJ17481 | Horto Florestal | RJ | 15.60 | 3.30 | 4.10 | 1.10 | 2.60 | 1.00 | 1.40 | 6.20 | 6.30 | 7.20 | 0.40 | 0.40 | 3.50 | 3.60 | 2.60 |
|
MNRJ17482 | Horto Florestal | RJ | 16.20 | 3.50 | 2.60 | 1.30 | 2.60 | 1.00 | 1.90 | 6.50 | 6.50 | 7.10 | 0.40 | 0.50 | 3.30 | 3.80 | 2.50 |
|
MNRJ17484 | Horto Florestal | RJ | 19.15 | 3.57 | 4.82 | 1.35 | 2.84 | 1.44 | 1.69 | 7.45 | 7.35 | 8.23 | 0.34 | 0.36 | 3.56 | 4.23 | 2.69 |
|
MNRJ17485 | Horto Florestal | RJ | 16.10 | 3.70 | 4.30 | 1.20 | 2.50 | 0.90 | 1.60 | 6.90 | 6.70 | 7.50 | 0.30 | 0.40 | 3.40 | 3.60 | 2.40 |
|
MNRJ17486 | Horto Florestal | RJ | 15.07 | 3.00 | 4.33 | 1.22 | 2.52 | 1.22 | 1.50 | 6.30 | 6.22 | 6.68 | 0.30 | 0.43 | 3.19 | 3.44 | 2.24 |
|
MNRJ17487 | Horto Florestal | RJ | 16.10 | 3.33 | 4.38 | 1.19 | 2.91 | 1.30 | 1.68 | 6.94 | 6.78 | 7.16 | 0.27 | 0.39 | 3.46 | 3.53 | 2.08 |
|
MNRJ17488 | Horto Florestal | RJ | 15.90 | 3.20 | 3.80 | 1.10 | 2.50 | 0.90 | 1.30 | 5.80 | 6.20 | 6.60 | 0.30 | 0.40 | 3.00 | 3.30 | 2.20 |
|
MNRJ17489 | Horto Florestal | RJ | 15.40 | 3.10 | 3.90 | 1.20 | 2.20 | 0.90 | 1.30 | 6.10 | 6.10 | 6.90 | 0.30 | 0.40 | 3.10 | 3.40 | 2.10 |
|
MNRJ17490 | Horto Florestal | RJ | 16.10 | 3.30 | 4.00 | 1.10 | 2.50 | 0.90 | 1.40 | 6.70 | 7.10 | 7.70 | 0.40 | 0.40 | 3.50 | 4.20 | 2.70 |
|
MNRJ17491 | Horto Florestal | RJ | 15.26 | 2.90 | 4.75 | 1.21 | 2.92 | 1.47 | 1.49 | 6.59 | 6.46 | 7.13 | 0.32 | 0.41 | 3.34 | 3.78 | 2.41 |
|
MNRJ17492 | Horto Florestal | RJ | 15.50 | 3.90 | 4.60 | 1.30 | 2.50 | 1.20 | 1.50 | 6.10 | 6.30 | 6.50 | 0.40 | 0.40 | 3.10 | 3.70 | 2.50 |
|
MNRJ17498 | Horto Florestal | RJ | 17.10 | 3.70 | 4.70 | 1.20 | 2.50 | 1.10 | 1.40 | 6.70 | 6.80 | 6.90 | 0.40 | 0.50 | 3.40 | 3.40 | 2.50 |
|
MNRJ17505 | Horto Florestal | RJ | 16.80 | 3.42 | 4.62 | 1.41 | 2.84 | 1.32 | 1.71 | 7.19 | 6.88 | 7.43 | 0.33 | 0.38 | 3.50 | 3.87 | 2.42 |
|
MNRJ17506 | Horto Florestal | RJ | 16.95 | 3.30 | 4.84 | 1.36 | 3.00 | 1.35 | 1.62 | 7.19 | 6.80 | 7.32 | 0.41 | 0.43 | 3.60 | 4.09 | 2.63 |
|
MNRJ17507 | Horto Florestal | RJ | 15.16 | 3.14 | 4.43 | 1.29 | 2.71 | 1.24 | 1.45 | 6.06 | 6.13 | 6.28 | 0.34 | 0.41 | 3.12 | 3.51 | 2.22 |
|
MNRJ17565 | Horto Florestal | RJ | 16.66 | 2.97 | 4.42 | 1.36 | 2.79 | 1.30 | 1.47 | 7.05 | 6.69 | 7.14 | 0.49 | 0.43 | 3.23 | 3.97 | 2.63 |
|
MNRJ66497 | Mimoso do Sul | ES | 16.60 | 3.14 | 4.77 | 1.40 | 2.89 | 1.31 | 1.48 | 6.69 | 6.49 | 6.90 | 0.39 | 0.43 | 3.55 | 3.80 | 2.32 |
MNRJ29057 | Povoação | ES | 12.72 | 2.70 | 3.89 | 1.05 | 2.42 | 1.08 | 1.20 | 5.35 | 5.05 | 5.24 | 0.30 | 0.39 | 2.65 | 2.79 | 1.79 | |
MNRJ29058 | Povoação | ES | 13.23 | 2.68 | 3.90 | 1.12 | 2.47 | 1.10 | 1.12 | 5.28 | 4.91 | 5.17 | 0.34 | 0.42 | 2.52 | 2.78 | 1.73 | |
MNRJ29059 | Povoação | ES | 13.56 | 2.53 | 3.63 | 1.15 | 2.37 | 1.08 | 1.12 | 5.36 | 5.25 | 5.33 | 0.29 | 0.39 | 2.80 | 3.00 | 1.89 | |
MNRJ29060 | Povoação | ES | 12.59 | 2.55 | 3.79 | 1.08 | 2.41 | 1.08 | 1.16 | 5.12 | 4.67 | 4.56 | 0.32 | 0.32 | 2.48 | 2.48 | 1.67 | |
MNRJ29073 | Povoação | ES | 13.76 | 2.53 | 3.94 | 1.25 | 2.43 | 1.18 | 1.29 | 5.67 | 5.63 | 5.88 | 0.41 | 0.41 | 2.88 | 3.07 | 1.85 | |
MNRJ29074 | Povoação | ES | 13.47 | 2.33 | 3.72 | 1.18 | 2.27 | 1.08 | 1.15 | 5.24 | 5.27 | 5.60 | 0.32 | 0.40 | 2.73 | 3.02 | 1.81 | |
MBML2858 | Povoação | ES | 13.47 | 2.54 | 3.94 | 0.88 | 2.04 | 0.82 | 1.03 | 5.11 | 5.39 | 5.50 | 0.33 | 0.43 | 2.61 | 2.91 | 1.72 | |
MBML2866 | Povoação | ES | 12.15 | 2.24 | 3.34 | 1.05 | 2.16 | 0.72 | 0.95 | 5.26 | 5.11 | 5.33 | 0.27 | 0.39 | 2.55 | 2.75 | 1.62 | |
MBML2863 | Povoação | ES | 12.30 | 2.34 | 3.75 | 0.82 | 2.00 | 0.83 | 0.93 | 5.22 | 5.32 | 5.42 | 0.31 | 0.48 | 2.50 | 2.63 | 1.59 | |
MZUSP147473 | Flona Rio Preto | ES | 16.07 | 3.18 | 3.87 | 1.09 | 2.18 | 1.09 | 1.33 | 6.01 | 6.00 | 6.41 | 0.24 | 0.23 | 3.15 | 3.30 | 2.07 | |
MZUSP147478 | Flona Rio Preto | ES | 15.71 | 2.75 | 4.52 | 1.30 | 2.80 | 1.13 | 1.25 | 6.55 | 6.47 | 6.91 | 0.35 | 0.44 | 3.21 | 3.45 | 2.33 | |
MZUSP147471 | Flona Rio Preto | ES | 14.43 | 2.75 | 3.83 | 1.03 | 2.57 | 1.11 | 1.51 | 6.07 | 6.27 | 6.23 | 0.25 | 0.26 | 2.99 | 2.84 | 1.83 | |
MZUSP147475 | Flona Rio Preto | ES | 14.48 | 2.79 | 4.55 | 1.28 | 2.55 | 1.28 | 1.25 | 6.63 | 6.41 | 6.73 | 0.36 | 0.36 | 3.26 | 3.48 | 2.16 | |
MZUSP147494 | Flona Rio Preto | ES | 16.55 | 3.14 | 4.63 | 1.36 | 2.55 | 1.24 | 1.36 | 7.02 | 6.89 | 7.59 | 0.25 | 0.37 | 3.33 | 3.80 | 2.40 | |
MZUSP147476 | Flona Rio Preto | ES | 16.09 | 2.70 | 4.36 | 1.24 | 2.65 | 1.31 | 1.36 | 6.73 | 6.26 | 6.11 | 0.32 | 0.47 | 2.95 | 3.33 | 1.83 | |
MZUSP147472 | Flona Rio Preto | ES | 15.28 | 2.80 | 4.08 | 1.33 | 2.67 | 1.24 | 1.33 | 7.08 | 6.55 | 6.60 | 0.34 | 0.41 | 3.20 | 3.40 | 2.22 | |
sp. | MTR13495 | Trancoso | BA | 15.92 | 3.39 | 4.07 | 1.06 | 2.65 | 0.94 | 1.43 | 6.37 | 6.16 | 6.77 | 0.27 | 0.42 | 3.60 | 3.45 | 2.06 |
sp. | MTR13547 | Trancoso | BA | 13.80 | 3.53 | 4.09 | 0.97 | 2.36 | 1.00 | 1.35 | 5.59 | 5.68 | 5.72 | 0.27 | 0.35 | 2.95 | 3.18 | 2.27 |
sp. | MTR13545 | Trancoso | BA | 15.07 | 3.51 | 4.24 | 1.10 | 2.43 | 1.03 | 1.35 | 5.91 | 6.41 | 6.78 | 0.29 | 0.38 | 2.95 | 3.40 | 2.35 |
sp. | MTR13590 | Trancoso | BA | 15.48 | 3.48 | 3.99 | 1.17 | 2.51 | 0.80 | 1.32 | 6.01 | 6.22 | 6.69 | 0.30 | 0.45 | 3.11 | 3.73 | 2.41 |
sp. | MTR13565 | Trancoso | BA | 16.27 | 3.78 | 4.31 | 0.95 | 2.59 | 1.04 | 1.61 | 5.64 | 6.33 | 6.56 | 0.33 | 0.46 | 3.20 | 3.68 | 2.67 |
sp. | MTR13548 | Trancoso | BA | 15.36 | 3.34 | 4.57 | 1.06 | 2.52 | 1.12 | 1.09 | 6.44 | 6.62 | 6.72 | 0.30 | 0.40 | 3.19 | 3.64 | 2.36 |
sp. | MTR13546 | Trancoso | BA | 15.53 | 3.10 | 4.40 | 1.24 | 2.30 | 0.91 | 1.46 | 6.02 | 5.96 | 6.56 | 0.31 | 0.40 | 2.90 | 3.30 | 2.14 |
sp. | MTR13489 | Trancoso | BA | 15.34 | 3.53 | 4.05 | 1.16 | 2.35 | 1.00 | 1.36 | 6.67 | 6.54 | 6.73 | 0.27 | 0.44 | 3.40 | 3.46 | 2.40 |
Samples included in the molecular analyses and genbank numbers. Numbers in bold were retrieved from previous studies.
|
|
|
|
|
|
|
|
|
|
MZUSP147497 | CTA1861 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147498 | CTA1863 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147499 | CTA1864 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147468 | CTA1865 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147482 | CTA1869 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147469 | CTA1870 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147500 | CTA1871 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147510 | CTA1872 | Serra | ES |
|
|
|
|
|
MZUSP147512 | CTA1874 | Serra | ES |
|
|
|
|
|
MZUSP147513 | CTA1875 | Serra | ES |
|
|
|
|
|
JFT479 | CTA1876 | Serra | ES |
|
|
|
|
|
MZUSP147514 | CTA1877 | Serra | ES |
|
|
|
|
|
JFT483 | CTA1879 | Serra | ES |
|
|
|
|
|
JFT499 | CTA1881 | Serra | ES |
|
|
|
|
|
MZUSP147520 | CTA1893 | Serra | ES |
|
|
|
|
|
MZUSP147521 | CTA1894 | Serra | ES |
|
|
|
|
|
MZUSP147523 | CTA1896 | Serra | ES |
|
|
|
|
|
MTR12276 | CTMZ6907 | FloNa dos Goytacazes | ES |
|
|
- |
|
|
MTR12296 | CTMZ6908 | FloNa dos Goytacazes | ES |
|
|
|
|
|
MTR12407 | CTMZ6912 | Reserva Natural Vale | ES |
|
|
- | - |
|
MTR12484 | CTMZ6920 | Reserva Natural Vale | ES |
|
|
- |
|
|
MTR12485 | CTMZ6921 | Reserva Natural Vale | ES |
|
|
- |
|
|
- | CTMZ6939 | Guarapari | ES |
|
|
- |
|
|
MTR12076 | MTR12076 | Reserva Natural Vale | ES |
|
|
|
|
|
MTR12297 | MTR12297 | FloNa dos Goytacazes | ES |
|
|
|
|
|
CFBH32057 | CFBH15784 | Ilhéus | BA |
|
|
|
|
|
MZUSP147496 | CTA1935 | Ituberá | BA |
|
|
|
|
|
MTR22122 | MTR22122 | EE Wenceslau Guimarães | BA |
|
|
|
|
|
MTR22123 | MTR22123 | EE Wenceslau Guimarães | BA |
|
|
|
|
|
PEU137 | PEU137 | Jaguaripe | BA |
|
|
|
|
|
PEU146 | PEU146 | Jaguaripe | BA |
|
|
|
|
|
MTR6001 | CTMZ6898 | Serra do Teimoso | BA |
|
|
|
|
|
- | CTMZ6900 | Ilhéus | BA |
|
|
|
|
|
- | CTMZ6901 | Ilhéus | BA |
|
|
|
|
|
MTR16070 | MTR16070 | Serra Bonita | BA |
|
|
|
|
|
- | CFBH73 | Picinguaba | SP |
|
|
|
|
|
CFBH17495 | CFBH7361 | Ilha de São Sebastião | SP |
|
|
- | |
|
- | CFBH76 | Picinguaba | SP |
|
|
|
|
|
JFT981 | CTA1934 | Matada Usina Paineiras | ES |
|
|
|
|
|
- | CTMZ6946 | Bertioga | SP |
|
|
- |
|
|
RN7003 | CTRN173 | Angra dos Reis | RJ |
|
|
- | - |
|
RN7004 | CTRN174 | Angra dos Reis | RJ |
|
|
- |
|
|
RN7005 | CTRN175 | Angra dos Reis | RJ |
|
|
- |
|
|
MNRJ47477 | MNRJ47477 | ReBio União | RJ |
|
|
- | - |
|
MNRJ48415 | MNRJ48415 | ReBio União | RJ |
|
|
- | - |
|
MNRJ49302 | MNRJ49302 | Cachoeiras de Macacu | ES |
|
|
|
|
|
MNRJ60744 | MNRJ60744 | Duque de Caxias | RJ |
|
- |
|
|
|
MNRJ66494 | MNRJ66494 | Mimoso do Sul | ES |
|
|
|
|
|
CFBH19029 | CFBH8594 | PE Ilha do Cardoso | SP |
|
|
- | - |
|
MZUSP136053 | CTMZ2485 | PE Carlos Botelho | SP |
|
|
- | - |
|
MZUSP136055 | CTMZ2493 | PE Carlos Botelho | SP |
|
- | - |
|
|
MZUSP136059 | CTMZ2497 | PE Carlos Botelho | SP | - |
|
- |
|
|
MTR7128 | CTMZ6943 | Fazenda Intervales | SP | - | - |
|
|
|
- | CTMZ6944 | Piedade | SP |
|
- |
|
- |
|
- | CTMZ6891 | Serra do Brigadeiro | MG |
|
|
- |
|
|
UFMG-A9643 | UFMG-T1802 | Ouro Branco | MG |
|
|
|
- |
|
UFMG-A9659 | UFMG-T1804 | Ouro Branco | MG |
|
|
- |
|
|
UFMG-A9651 | UFMG-T1810 | Ouro Branco | MG |
|
|
- |
|
|
UFMG-A9656 | UFMG-T1815 | Ouro Branco | MG |
|
|
- |
|
- | CFBH1437 | ReBio Sooretama | ES |
|
|
- |
|
|
- | CFBH1438 | ReBio Sooretama | ES |
|
|
- |
|
|
CFBH19471 | CFBH9055 | Povoação | ES |
|
|
|
|
|
CFBH18076 | CFBH9082 | Povoação | ES |
|
|
|
|
|
CFBH18077 | CFBH9083 | Povoação | ES |
|
|
|
|
|
JFT831 | CTA1906 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147471 | CTA1907 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147472 | CTA1908 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147473 | CTA1909 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147474 | CTA1918 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147475 | CTA1919 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147494 | CTA1923 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147479 | CTA1929 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147480 | CTA1931 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147493 | CTA1933 | FloNa do Rio Preto | ES |
|
|
|
|
|
JFT990 | CTA1938 | PE de Itaúnas | ES | - |
|
- |
|
|
MTR12017 | CTMZ6903 | Reserva Natural Vale | ES |
|
|
- |
|
|
MTR12077 | CTMZ6905 | Reserva Natural Vale | ES |
|
|
- | - | |
MTR12470 | CTMZ6916 | Reserva Natural Vale | ES |
|
|
- |
|
|
MTR12471 | CTMZ6917 | Reserva Natural Vale | ES |
|
|
- |
|
|
MTR21527 | LGA3267 | ReBio Córrego Veado | ES |
|
|
|
|
|
|
CFBH9331 | CFBH2078 | ReBio Sooretama | ES |
|
|
|
|
|
CFBH18075 | CFBH9060 | Povoação | ES |
|
|
|
|
|
CFBH 22501 | CTA1860 | ReBio Duas Bocas | ES |
|
|
|
|
|
MZUSP147485 | CTA1887 | FloNa do Rio Preto | ES |
|
|
|
|
|
MZUSP147487 | CTA1924 | FloNa do Rio Preto | ES |
|
|
|
|
|
MTR12094 | CTMZ6906 | FloNa dos Goytacazes | ES |
|
|
|
|
|
LGA2630 | LGA2630 | ReBio Córrego Veado | ES |
|
|
|
|
|
MTR12266 | MTR12266 | FloNa dos Goytacazes | ES |
|
|
|
|
|
MTR17524 | MTR17524 | PE do Rio Doce | MG |
|
|
|
|
|
MTR17571 | MTR17571 | PE do Rio Doce | MG |
|
|
|
|
sp. | - | CFBH15818 | Porto Seguro | BA | - |
|
- |
|
sp. | MTR13466 | CTMZ6923 | Trancoso | BA |
|
|
|
|
sp. | MTR13489 | CTMZ6924 | Trancoso | BA |
|
|
|
|
sp. | MTR13495 | CTMZ6925 | Trancoso | BA |
|
|
- |
|
sp. | MTR13545 | CTMZ6927 | Trancoso | BA |
|
|
- |
|
sp. | MTR13547 | CTMZ6929 | Trancoso | BA |
|
|
- |
|
sp. | MTR13548 | CTMZ6930 | Trancoso | BA |
|
|
- |
|
sp. | MTR13565 | CTMZ6932 | Trancoso | BA |
|
|
- |
|
sp. | MTR13579 | CTMZ6933 | Trancoso | BA |
|
|
- |
|
sp. | MTR13589 | CTMZ6935 | Trancoso | BA |
|
|
|
- |