Corresponding author: Mladen Kučinić (
Academic editor: R. Holzenthal
We describe a new autumnal caddisfly species
The genus
Four years ago we started systematically collecting adults of the genus
Localities where
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Bijele stijene | wellspring and stream | 144 |
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Gore | wellspring | 165 |
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Hrvatski Čuntić | stream | 159 |
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Marića točak | wellspring | 163 |
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Pašino vrelo | spring | 185 |
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Pecki | spring | 161 |
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Slabinja | wellspring | 104 |
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Varoški bunar | wellspring | 130 |
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Type locality of
In pyramid-type emergence traps caddisflies were collected in 1% formaldehyde and thereafter stored in 80% alcohol. All other collected specimens were stored directly in 80% or 96% alcohol. All specimens were deposited in the collections of the first and second authors. The holotype is deposited in the Croatian Natural History Museum in Zagreb.
Systematic presentation follows
List of species included in the DNA analysis (mtCOI sequences). Localities are given with country code, locality/specimen data, and collection date.<br/>
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ROU, Ignis Mts., springs near Desesti-Statiunea Izvoare, 21.10.2010 | CAxJC0101 |
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Ecsedi, Olah & Szivak |
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ROU, Ignis Mts., springs near Desesti-Statiunea Izvoare, 21.10.2010 | CAxJC0102 |
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Ecsedi, Olah & Szivak |
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ROU, Ignis Mts., springs near Desesti-Statiunea Izvoare, 21.10.2010 | CAxJC0103 |
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Ecsedi, Olah & Szivak |
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BIH, Livno, Sturba river, 08.11.2009 | CBxED0101 | Kučinić, Delić & Mihoci | |
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BIH, Livno, Sturba river, 08.11.2009 | CBxED0102 | Kučinić, Delić & Mihoci | |
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BIH, Livno, Sturba river, 08.11.2009 | CBxED0103 | Kučinić, Delić & Mihoci | |
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BIH, Livno, Sturba river, 08.11.2009 | CBxED0104 | Kučinić, Delić & Mihoci | |
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BIH, Livno, Sturba river, 08.11.2009 | CBxED0105 | Kučinić, Delić & Mihoci | |
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SLO, Ljubljana, Mostec park, Przanec stream, 06.12.2009 | CCxEA0101 |
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Dery & Szivak |
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SLO, Ljubljana, Mostec park, Przanec stream, 06.12.2009 | CCxEA0102 |
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Dery & Szivak |
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SLO, Ljubljana, Mostec park, Przanec stream, 06.12.2009 | CCxEA0103 |
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Dery & Szivak |
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SLO, Ljubljana, Mostec park, Przanec stream, 06.12.2009 | CCxEA0104 |
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Dery & Szivak |
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SLO, Ljubljana, Mostec park, Przanec stream, 06.12.2009 | CCxEA0105 |
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Dery & Szivak |
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SLO, spring of Lokavscek stream near Predmeja, 06.12.2009 | CGREG0101 |
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Dery & Szivak |
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SLO, spring of Lokavscek stream near Predmeja, 06.12.2009 | CGREG0102 |
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Dery & Szivak |
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SLO, spring of Lokavscek stream near Predmeja, 06.12.2009 | CGREG0103 |
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Dery & Szivak |
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SLO, spring of Lokavscek stream near Predmeja, 06.12.2009 | CGREG0104 |
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Dery & Szivak |
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SLO, spring of Lokavscek stream near Predmeja, 06.12.2009 | CGREG0105 |
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Dery & Szivak |
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SLO, spring near Čekovnik (Hlevise), 05.12.2009 | CGREG0201 |
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Dery & Szivak |
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SLO, spring near Čekovnik (Blask), 05.12.2009 | CGREG0301 |
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Dery & Szivak |
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SLO, spring near Čekovnik (Blask), 05.12.2009 | CGREG0302 |
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Dery & Szivak |
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SLO, spring near Čekovnik (Blask), 05.12.2009 | CGREG0303 |
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Dery & Szivak |
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SLO, spring near Čekovnik (Blask), 05.12.2009 | CGREG0304 |
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Dery & Szivak |
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SLO, Susica stream near Misliče, 06.12.2009 | CIxEI0101 |
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Dery & Szivak |
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SLO, Susica stream near Misliče, 06.12.2009 | CIxEI0102 |
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Dery & Szivak |
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SLO, Misliče, Susica stream, 06.12.2009 | CIxEI0103 |
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Dery & Szivak |
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SLO, Misliče, Susica stream, 06.12.2009 | CIxEI0104 |
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Dery & Szivak |
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SLO, Misliče, Susica stream, 06.12.2009 | CIxEI0105 |
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Dery & Szivak |
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HUN, Mecsek Mts., Vár valley, Pásztor spring 05.11.2010 | CMJKB0101 |
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Olah, Szivak & Uherkovich |
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HUN, Mecsek Mts., Vár valley, Pásztor spring 05.11.2010 | CMJKB0102 |
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Olah, Szivak & Uherkovich |
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HUN, Mecsek Mts., Vár valley, Pásztor spring 05.11.2010 | CMJKB0103 |
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Olah, Szivak & Uherkovich |
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HUN, Mecsek Mts., Vár valley, Pásztor spring 05.11.2010 | CMJKB0104 |
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Olah, Szivak & Uherkovich |
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AUT, valley Hottmannsgraben, Unteraspang (Aspang Markt) 19.11.2009 | CMJDJ0101 |
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Dery & Szivak |
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CRO, Kompanj, 14.11.2009 | CMREI0101 |
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Kučinić & Vučković |
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CRO, Kompanj, 14.11.2009 | CMREI0102 |
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Kučinić & Vučković |
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CRO, Kompanj, 14.11.2009 | CMREI0103 |
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Kučinić & Vučković |
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CRO, Kompanj, 14.11.2009 | CMREI0104 |
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Kučinić & Vučković |
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CRO, Kompanj, 14.11.2009 | CMREI0105 |
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Kučinić & Vučković |
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HUN, Mecsek Mts., Nagy-Mély valley, Kánya spring, 14.11.2009 | CRMKB0101 |
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Szivak |
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HUN, Mecsek Mts., Vár valley, Pásztor spring, 06.11.2009 | CRMKB0201 |
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Szivak & Uherkovich |
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HUN, Mecsek Mts., Melegmányi valley, Mésztufa spring, 14.11.2009 | CRMKB0301 |
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Szivak |
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HUN, Mecsek Mts., Vár valley, Iharos spring, 06.11.2009 | CRMKB0401 |
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Szivak |
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AUT, Saualpe, Klieningbach stream near Kliening, 21.11.2009 | CRNDI0101 |
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Dery & Szivak |
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AUT, Saualpe, springs of the Klippitzbach stream near Klippitztörl 21.11.2009 | CRNDI0201 |
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Dery & Szivak |
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AUT, Saualpe, springs of the Klippitzbach stream near Klippitztörl 21.11.2009 | CRNDI0202 |
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Dery & Szivak |
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AUT, Saualpe, springs of the Klippitzbach stream near Klippitztörl 21.11.2009 | CRNDI0203 |
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Dery & Szivak |
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AUT, Saualpe, springs of the Klippitzbach stream near Klippitztörl 21.11.2009 | CRNDI0204 |
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Dery & Szivak |
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AUT, Saulape, spring of the Löllingbach stream near Stranach, 21.11.2009 | CRNDI0301 |
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Dery & Szivak |
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AUT, Saulape, spring of the Löllingbach stream near Stranach, 21.11.2009 | CRNDI0302 |
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Dery & Szivak |
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AUT, Saulape, spring of the Löllingbach stream near Stranach, 21.11.2009 | CRNDI0303 |
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Dery & Szivak |
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AUT, Saulape, spring of the Löllingbach stream near Stranach, 21.11.2009 | CRNDI0304 |
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Dery & Szivak |
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HUN, Kőszegi Mts., Hörmann spring near Velem, 18.11.2009 | CRRDJ0101 | Szivak | |
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HUN, Kőszegi Mts., Hörmann spring near Velem, 18.11.2009 | CRRDJ0102 | Szivak | |
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AUT, Mitterneuwald, Hermann spring, 19.11.2009 | CRRDJ0201 |
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Dery & Szivak |
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AUT, Sommeralm, Mixnitzbach stream, 20.11.2009 | CRRDJ0301 | Dery & Szivak | |
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AUT, Sommeralm, Mixnitzbach stream, 20.11.2009 | CRRDJ0302 |
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Dery & Szivak |
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AUT, Hochegg bei Grimmenstein, spring and its outlet, 19.11.2009 | CRRDJ0401 |
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Dery & Szivak |
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AUT, Hochegg bei Grimmenstein, spring and its outlet, 19.11.2009 | CRRDJ0402 |
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Dery & Szivak |
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AUT, Hochegg bei Grimmenstein, spring and its outlet, 19.11.2009 | CRRDJ0403 |
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Dery & Szivak |
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AUT, Ausserneuwald, spring, 19.11.2009 | CRRDJ0501 |
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Dery & Szivak |
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AUT, Ausserneuwald, spring, 19.11.2009 | CRRDJ0502 |
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Dery & Szivak |
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AUT, Plenzengreith, upper reach of stream Schöcklbach, 20.11.2009 | CRRDJ0601 |
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Dery & Szivak |
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AUT, Plenzengreith, upper reach of stream Schöcklbach, 20.11.2009 | CRRDJ0602 |
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Dery & Szivak |
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AUT, Plenzengreith, upper reach of stream Schöcklbach, 20.11.2009 | CRRDJ0603 |
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Dery & Szivak |
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SLO, Pohorje Mts., Osankarica (Lukanja), 10.11.2008 | CRRDG0101 |
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Popijač |
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SLO, Pohorje Mts., Osankarica (Lukanja), 10.11.2008 | CRRDG0102 |
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Popijač |
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SLO, Pohorje Mts., Osankarica (Lukanja), 10.11.2008 | CRRDG0103 |
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Popijač |
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CRO, Medvednica Mts., Mrzlak spring near Sljeme, 18.11.2006 | CRREE0101 |
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Popijač |
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CRO, Medvednica Mts., Mrzlak spring near Sljeme, 18.11.2006 | CRREE0102 |
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Popijač |
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CRO, Medvednica Mts., Kraljičin Zdenac spring, Kraljičin Zdenac, 19.11.2009 | CRREE0201 |
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Kučinić & Vučković |
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CRO, Medvednica Mts., Bliznec stream, Podsljeme (Pilana), 09.12.2009 | CRREE0301 |
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Kučinić & Vučković |
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CRO, Žumberak Mts., Slapnica stream, Ribička kuća, 28.10.2009 | CRREF0101 |
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Kučinić & Vučković |
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ROU, spring brook in Cerna valley near Tatu, 13.11.2010 | CRSJF0101 |
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Ecsedi & Szivak |
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ROU, spring brook in Cerna valley near Tatu, 13.11.2010 | CRSJF0102 |
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Ecsedi & Szivak |
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ROU, spring brook in Cerna valley near Tatu, 13.11.2010 | CRSJF0103 |
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Ecsedi & Szivak |
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SRB, Derdap Mts., stream valley N of Golubinje, 13.10.2006 | CRSGE0101 |
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Danyi, Kontschan & Muranyi |
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SRB, Derdap Mts., stream valley N of Golubinje, 13.10.2006 | CRSGE0102 |
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Danyi, Kontschan & Muranyi |
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SRB, Derdap Mts.,Grgeci spring, Donji Milankovac, 13.10.2006 | CRSGE0201 |
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Danyi, Kontschan & Muranyi |
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SRB, Derdap Mts.,Grgeci spring, Donji Milankovac, 13.10.2006 | CRSGE0203 |
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Danyi, Kontschan & Muranyi |
CRO, Kriz spring near Petrinja, 08.12.2009 | CxxEC0101 |
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Kučinić, Delić & Bučar | |
CRO, Kriz spring near Petrinja, 07.11.2009 | CxxEC0102 |
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Kučinić, Delić & Bučar | |
CRO, Kriz spring near Petrinja, 07.11.2009 | CxxEC0103 |
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Kučinić, Delić & Bučar | |
CRO, Kriz spring near Petrinja, 04.11.2009 | CxxEC0104 |
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Bučar | |
CRO, Kriz spring near Petrinja, 08.12.2009 | CxxEC0105 |
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Kučinić, Delić, Bučar & Vučković | |
CRO, Hrvatski Cuntic, Marića točak spring, 22.11.2009 | CxxEC0201 |
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Kučinić, Delić & Bučar | |
CRO, Hrvatski Cuntic, Marića točak spring, 21.11.2009 | CxxEC0202 |
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Kučinić, Delić & Bučar | |
CRO, Hrvatska Kostajnica, Varoški bunar spring, 06.12.2009 | CxxEC0301 | Kučinić, Delić & Bučar | ||
CRO, Šuplji Kamen, Slabinja spring, 29.11.2009 | CxxEC0401 |
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Kučinić, Delić & Bučar | |
CRO, Banovina region, Pecki spring, 15.12.2009 | CxxEC0501 |
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Kučinić, Delić & Bučar | |
CRO, Banovina region, Pecki spring, 21.11.2009 | CxxEC0502 |
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Kučinić, Delić & Bučar | |
CRO, Banovina region, Pecki spring, 21.11.2009 | CxxEC0503 |
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Kučinić, Delić & Bučar | |
CRO, Banovina region, Gora spring, 10.12.2009 | CxxEC0601 |
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Bučar | |
CRO, Mečenčani, Pašino vrelo, 29.11.2009 | CxxEC0701 |
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Kučinić, Delić & Bučar | |
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AUT, Lower Austria, Rohrwiesteich, 20.10.2004 | 08HMCAD-331* | Malicky | |
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AUT, Lower Austria, Rohrwiesteich, 20.10.2004 | 08HMCAD-333* | Malicky | |
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ITA, Umbria, Perugia, Fium Nera above Visso,11.12.2005 | 07HMCAD-0177* | Malicky | |
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ITA, Belluno, Val Canzoi, Veneto, 31.10.2003 | HM09Cm7* | Malicky | |
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AUT, Lower Austria, Sarleinsbach, 27.06.2005 | 07HMCAD-0134* | Malicky | |
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IT, Valprato Soana, Ronchietto, 10.07.2004 | HM09Dalp8* | Delmaistro | |
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SK, Lower Tatra, Stream above Partizanska L'upča, 09.06.2008 | ESCAD909-17* | Bonada | |
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ES, Camprodon/Setcases Alta Val de Ter, 27.07.2004 | HM09Drec8* | Aistleitner | |
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AUT, Carinthia, Valentinbach, Plockenstrasse, 08.07.2007 | 08HMCAD-020* | Malicky | |
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NORWAY | NHRS:FI9 |
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Malm & Johanson 2011 |
The mitochondrial COI barcodes were generated at the Canadian Centre for DNA Barcoding, University of Guelph, Canada. Standard barcoding protocols for DNA extraction (
Bayesian tree for members of the
Estimates of evolutionary divergence over sequence pairs within and between phylogenetically defined species and subspecies based on mtCOI sequence data. Distance matrix is shown with the mean ± SD values of the intraspecific and interspecific pairwise genetic distances for the all
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1.05±0.97 | 1.61±0.49 | 3.02±0.17 | 4.87±0.24 | 4.55±0.24 | 4.79±0.27 | 4.63±0.30 | 5.44±0.39 | 9.24±0.46 | 12.64±0.15 | 12.85±0.33 | 12.69±0.20 | 12.69±0.20 | 14.03±0.20 | 12.00±0.39 | 12.32±0.20 | 14.17±0.16 |
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1.20±0.89 | 2.83±0.31 | 5.06±0.23 | 4.74±0.24 | 5.26±0.20 | 5.17±0.18 | 5.85±0.11 | 9.45±0.27 | 12.85±0.08 | 12.66±0.12 | 12.83±0.12 | 12.83±0.12 | 14.06±0.12 | 12.18±0.14 | 11.98±0.24 | 14.14±0.39 | |
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0.17±0.11 | 4.79±0.09 | 4.47±0.10 | 4.69±0.12 | 4.38±0.11 | 4.66±0.13 | 8.87±0.07 | 11.89±0.07 | 11.97±0.08 | 11.76±0.14 | 11.76±0.14 | 12.44±0.14 | 11.60±0.12 | 11.47±0.08 | 13.78±0.00 | ||
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0.00±0.00 | 0.37±0.07 | 3.80±0.08 | 5.86±0.14 | 5.69±0.06 | 9.92±0.00 | 12.61±0.00 | 12.61±0.00 | 12.77±0.00 | 12.77±0.00 | 13.61±0.00 | 12.77±0.00 | 12.61±0.00 | 14.49±0.07 | |||
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0.03±0.07 | 3.48±0.10 | 5.54±0.15 | 5.37±0.08 | 9.93±0.05 | 12.62±0.05 | 12.62±0.05 | 12.79±0.05 | 12.79±0.05 | 13.63±0.05 | 12.78±0.05 | 12.62±0.05 | 14.17±0.08 | ||||
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0.10±0.09 | 4.41±0.16 | 5.11±0.18 | 10.15±0.08 | 12.67±0.08 | 13.21±0.09 | 12.54±0.09 | 12.54±0.09 | 13.04±0.09 | 13.38±0.09 | 13.55±0.09 | 15.39±0.11 | |||||
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0.17±0.13 | 2.02±0.20 | 8.79±0.13 | 12.48±0.13 | 12.65±0.14 | 12.48±0.14 | 12.48±0.14 | 12.65±0.14 | 11.21±0.08 | 12.89±0.08 | 14.00±0.16 | ||||||
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0.75±0.57 | 8.72±0.06 | 12.24±0.06 | 13.04±0.13 | 12.05±0.21 | 12.05±0.21 | 12.67±0.23 | 11.56±0.22 | 13.93±0.06 | 14.44±0.15 | |||||||
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0.00±0.00 | 11.60±0.00 | 11.60±0.00 | 11.93±0.00 | 11.93±0.00 | 13.11±0.00 | 11.76±0.00 | 12.94±0.00 | 12.77±0.18 | ||||||||
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0.00±0.00 | 2.69±0.00 | 4.87±0.00 | 4.87±0.00 | 6.05±0.00 | 12.61±0.00 | 12.61±0.00 | 15.26±0.17 | |||||||||
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0.00±0.00 | 5.55±0.00 | 5.55±0.00 | 6.55±0.00 | 12.94±0.00 | 11.76±0.00 | 14.82±0.08 | ||||||||||
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0.00±0.00 | 0.00±0.00 | 5.88±0.00 | 13.05±0.19 | 12.94±0.00 | 15.83±0.08 | |||||||||||
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0.00±0.00 | 5.88±0.00 | 13.05±0.19 | 12.94±0.00 | 15.83±0.08 | ||||||||||||
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0.00±0.00 | 13.45±0.00 | 13.44±0.00 | 15.87±0.09 | |||||||||||||
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0.35±0.00 | 11.71±0.19 | 13.44±0.11 | ||||||||||||||
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0.00±0.00 | 13.04±0.15 | |||||||||||||||
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0.47±0.51 |
Male of
Wings and legs yellow to yellowish-brown; veins darker in both sexes (
Male genitalia (
Female genitalia (
The species is dedicated to Professor Matija Bučar from the Faculty of Education, Department in Petrinja, University of Zagreb.
During our recent faunal surveys in Croatia and the Western Dinaric Balkan
In addition to
In both sexes, especially in the adult female,
Based on the phylogenetic position of
Research on the diversity of large karst springs on the Balkan Peninsula has revealed high levels of caddisfly diversity. In some cases more than 20 species were collected from a single spring (
Distribution of
Until now, the new species
According to the current findings,
Taxa from the
Many members of the genus
We express our gratitude to Xin Zhou for handling the mitochondrial barcoding of our specimens within the Barcoding of Life initiative. We thank Karl Kjer for sharing the previously unpublished “outgroup” data from BOLD. We thank two anonymous reviewers and Ralph Holzenthal for their valuable comments that helped improve the manuscript. For technical help we acknowledge senior curator Iva Mihoci, PhD, from the Croatian Natural Museum in Zagreb, Nediljko Landeka form the Institute of Public Health in Pula, and Milivoj Franjević from the Foresty Faculty, University of Zagreb. ISz was partially funded through an incoming research grant of the Biodiversity and Climate Research Centre in the frame of the research funding program Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE) of Hesse's Ministry of Higher Education, Research, and the Arts, Germany. SUP and MB acknowledge financial support from the research fundprogram “LOEWE – Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse's Ministry of Higher Education, Research, and the Arts. This research was supported by the Austrian Science Fund (FWF) as a part of Project P 23687-B17 and Croatian Ministry of Science, Education and Sports as a part of Project No. 119–1193080–1206.