A new genus and species of Laelapidae from Iran with notes on Gymnolaelaps Berlese and Laelaspisella Marais &amp; Loots (Acari, Mesostigmata)

Alireza Nemati; Dariusz J. Gwiazdowicz

doi:10.3897/zookeys.549.6891

Research Article

A new genus and species of Laelapidae from Iran with notes on Gymnolaelaps Berlese and Laelaspisella Marais & Loots (Acari, Mesostigmata)

Alireza Nemati^‡, Dariusz J. Gwiazdowicz^§

‡ University of Shahrekord, Shahrekord, Iran

§ Poznań University of Life Sciences, Poznań, Poland

Corresponding author: Alireza Nemati ( alireza.nemat@ymail.com )

Academic editor: Farid Faraji

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Nemati A, Gwiazdowicz DJ (2016) A new genus and species of Laelapidae from Iran with notes on Gymnolaelaps Berlese and Laelaspisella Marais & Loots (Acari, Mesostigmata). ZooKeys 549: 23-49. https://doi.org/10.3897/zookeys.549.6891

ZooBank: urn:lsid:zoobank.org:pub:DBBEAD94-CB3B-40AE-9B7B-1DF4AFFA81F9

Abstract

Pogonolaelaps gen. n. a new genus of Laelapidae Berlese is erected and described based on P. canestrinii (Berlese), comb. n. as well as on specimens which were collected from soil and ant nests in different parts of Iran. Also, a new species Pogonolaelaps beaulieui sp. n. is described based on specimens collected from soil, litter and ant nests in various parts of Chaharmahal va Bakhtiari province, Iran. The genus Gymnolaelaps is revised considering species with some morphological deviations. Gymnolaelaps reniculus (Karg, 1981) and Gymnolaelaps triquetrus (Karg, 2003) are removed from this genus and placed in their original genus Pseudoparasitus. The characters of Laelaspisella Marais & Loots, 1969 are discussed in a detail along with a proposal of a new definition.

Keywords

Taxonomy, Pogonolaelaps gen. n., ant nest, Gymnolaelaps , Laelaspisella

Introduction

The Laelapidae Berlese comprises a multitude of morphologically and behaviorally diverse mite groups that are free-living or associated with arthropods, mammals and birds (e.g. Strong and Halliday 1994, Faraji and Halliday 2009, Lindquist et al. 2009). Hypotheses concerning the evolutionary history of this family and its relatives are minimally developed and the classification of the group is consequently inadequate (Casanueva 1993). This family is considered to include different subfamilies, genera and subgenera by different authors (Berlese 1903, 1923, Baker and Wharton 1952, Van Aswegen and Loots 1970, Karg 1978, 1979, 1982, 1993, 2000, Krantz and Ainscough 1990, Casanueva 1993, Krantz 1998). Lindquist et al. (2009) considered nine subfamilies for laelapid mites, based in part on a phylogenetic study of free-living and arthropod-associated taxa by Casanueva (1993), and the recently characterized subfamily Acanthochelinae (Radovsky and Gettinger 1999).

Gymnolaelaps was considered at different taxonomic levels: as a subgenus of Hypoaspis sensu lato (Berlese 1916, Evans and Till 1966, Hunter 1967), as a subgenus of Pseudoparasitus (Karg 1989, 1993) and as a distinct genus (Sellnick 1935, Evans and Till 1979, Joharchi et al. 2011; Joharchi and Halliday 2013). We herein consider Gymnolaelaps as a genus. The genus Gymnolaelaps has been collected in many parts of the world, mainly from ants’ nests. The genus includes approximately 35 described species (Joharchi et al. 2011). So far, a total of eight species notwithstanding their validity were reported from Iran: Gymnolaelaps canestrinii (Berlese), G. kabitae Bhattacharyya, G. laevis (Michael), G. messor Joharchi et al., G. myrmecophilus (Berlese), G. myrmophilus (Michael) and G. prestoni Joharchi et al., G. artavilensis Joharchi & Halliday (Joharchi and Halliday 2013).

Laelaspisella was erected by Marais and Loots (1969) in Laelapinae to accommodate two new species: Laelaspisella macrodorsalis Marais & Loots and L. epigynialis Marais & Loots collected from forest soil in South Africa, Lesotho and Congo. Subsequently, this genus was considered as a subgenus of Hypoaspis sens. lat. by Karg (1989), who described two additional species, Hypoaspis (Laelaspisella) foramenis Karg, 1989 and Hypoaspis (Laelaspisella) cavitatis Karg, 1982 and as distinct genus by Joharchi and Halliday (2013). The latter authors considered Laelaspisella canestrinii and L. kabitae as two more species of this genus but excluded two mentioned species of Karg of Laelaspisella (Joharchi and Halliday 2013).

During our survey on Mesostigmata mites inhabiting soil and litter, Pogonolaelaps canestrinii (Berlese), comb. n. from different habitats and localities was discovered. Based on that species, a new genus, Pogonolaelaps gen. n. is proposed and described. Also, a new species of Pogonolaelaps found in Iran is described. Redescription of Pogonolaelaps canestrinii comb. n. is given along with additional information on the specimens of this species in Berlese collection. The definition of Laelaspisella is also revised.

Materials and methods

Mites were collected from soil and litter samples from different parts of Iran. Mites were extracted using Berlese funnels, placed in lactic acid at 55 °C for clearing and then mounted in Hoyer’s medium on permanent microslides. Line drawings were made by the use of a drawing tube and figures were elaborated with Corel X-draw software, based on the scanned line drawings. All measurements are given in micrometers (μm). The dorsal setae notation, leg and palp chaetotaxy follows that of Lindquist and Evans (1965), Evans (1963a, b) and Evans and Till 1965 respectively. Terminology for idiosomal glands and lyrifissures follows Johnston and Moraza (1991). We have attempted to identify all pore-like structures, but we acknowledge that some may have been overlooked. The holotype and some of the paratypes (four females and two males) of the new species are deposited in the Acarological Laboratory, Department of Plant Protection, Agricultural College, Shahrekord University(APAS), Shahrekord, Iran. Three female paratypes are deposited in the Senckenberg Museum fur Naturkunde Görlitz, Am Museum, Görlitz, Germany; Natural History Museum Cromwell Road London SW7 5BD UK; and National Museum of Natural History(NMNH), Smithsonian Institute Washington D.C., 20013-7012 USA. One female paratype and one male are deposited in Poznan University of Life Sciences, Department of Forest Pathology Wojska Polskiego 71C, 60–625 Poznań, Poland. Redescription of Pogonolaelaps canestrinii comb. n. was based on the specimens in Acarological Laboratory, Department of Plant Protection, Agricultural College, Shahrekord University Shahrekord, Iran, which the figures have been compared with type specimens in the Berlese collection (Italy) by Dr. Roberto Nanelli.

Systematics

Genus Pogonolaelaps gen. n.

http://zoobank.org/94CC5068-5F2C-4222-8D3B-54DD32D3216D

Type species

Laelaps canestrinii Berlese, 1903, by original designation.

Genus diagnosis

Female with a three-tined palp tarsal claw; the dorsal seta of the chelicerae present, epistome smooth, corniculi horn-like, internal malae free medially and densely fringed with very elongate hairs, in addition possess two detachments of densely and very elongate hairs at basal part of each internal mala; st4 absent; genital shield large, abutting anal shield, with st5 on shield and three pairs of setae adjacent to the lateral edges; scimitar-shaped dorsal setae with small knob at their base, dorsal shield with holotrichous status on podonotal and hypertrichous on opisthonotal part, plus 0-3 unpaired setae between J series and 7-9 pairs of long tick setae on latero-posterior part of opisthonotal region; genu and tibia I with normal chaetotaxy (2 3/2 3/1 2), and genu IV with ten setae including two ventral setae (av and pv). Male with separate anal shield and strong spine-like seta on femur II.

Description

Idiosomal dorsum. Dorsal shield oval shaped, well sclerotized, nearly wraps around and overlaps onto the ventral idiosoma, podonotal part with holotrichous and opisthonotal with hypertrichous condition, shield with 51-55 pairs of setae, 28-32 pairs on opisthonotal region, plus 0-3 Jx setae between J series (usually with 3), rx seta present on podonotal part, setae increasing in length from anterior to posterior and from dorso-central to dorso-lateral part, latero-posterior part of opisthonotal region with 7-9 pairs of long, thick, barbed setae (Figs 11, 15), dorsal setae with a small basal knob (Fig. 17). Dorsal shield generally with six pairs of large slit-like lyrifissures (Figs 11, 15).

Idiosomal venter. Tritosternum with columnar base and paired free pilose laciniae; pre-sternal plates weakly sclerotized and ornamented with transverse lines (Figs 1, 2, 12, 16a). Sternal shield widest between coxae II and III, anterior margin sinuate, convex medially, posterior margin deeply concave (Figs 1, 2, 16a). With three slit-like iv1-3, all located on the surface of sternal shield (Fig. 16a); st4 absent (Figs 2, 16a). Endopodal plates II/III fused to lateral margins of sternal shield, endopodal plates III/IV elongate, curved. Large crescent-shape podal plates surrounding coxae IV, fused with contiguous exopodal plates and extended to the anterior level of coxae II (Figs 1, 16a).

Figures 1–6.

Pogonolaelaps canestrinii (Berlese). (Female): 1–2 Ventral idiosoma 3 Epigynal shield 4 Anal shield 5 End part of dorsal shield 6 Genu and tibia IV (ventral view). Scale bars: 100 µm.

Figures 7–10.

Pogonolaelaps canestrinii (Berlese). (Female): 7 Hypostome 8 Epistome 9 Chelicera 10 Leg IV.

Figures 11–14.

Pogonolaelaps canestrinii (Berlese). (Male): 11 Dorsal shield 12 Ventral idiosoma 13 Hypostome 14 Chelicera.

Figures 15–17.

Pogonolaelaps beaulieui Nemati & Gwiazdowicz, gen. n., sp. n. (Female): 15 Dorsal shield 16a, b Ventral idiosoma 17 An example of dorsal shield setae.

Genital shield broad, abutting anal shield, with one pair of setae (st5) on shield and three pairs of setae adjacent to lateral edges (Figs 1, 3, 16a); circular paragenital pores (iv5) located on soft integument between coxa IV and pair of minute narrow platelets (Figs 1, 16a). Anal shield subtriangular. Opisthogastric surface with: one pair of elongate metapodal plates, two pairs of minute platelets, 10-11 pairs of long setae, r6, Jv1-5 and Zv1-5 usually present. Peritreme extending from coxa IV to anterior level of coxa I (Fig. 16a), peritrematal shield wide, with two pairs of post-stigmatal pores, one pair of small pores anterior to stigmata and two pairs of pores (ip, gp) at level of coxae II/III (Fig. 16a at left side).

Gnathosoma. Deutosternal groove with six rows of denticles, corniculi horn-like, internal malae free medially and densely fringed, in addition possess two detachments of densely and very elongate hairs at basal part of each internal mala; labrum elongate, densely pubescent (Figs 7, 13, 18). Epistome sub-triangular with smooth antero-lateral margins (Figs 8, 19). Chelicerae chelate-dentate with prominent dorsal seta, lyrifissure, arthrodial processes and moderately robust setaceous pilus dentilis, movable digit with two teeth (Figs 9, 20). Digit-like male spermatodactyl simple and free distally (Figs 14, 28). Palp chaetotaxy normal (sensu Evans and Till 1965), numbers of setae on palp trochanter-tarsus: 2, 5, 6, 14 and 15 with aciculate and smooth setae, except al1-2 of palp genu aciculate and slightly thickened; palp-tarsal apotele three-tined, basal tine smaller (Figs 21–22).

Figures 18–22.

Pogonolaelaps beaulieui Nemati & Gwiazdowicz, gen. n., sp. n. (Female): 18 Hypostome 19 Epistome 20 Chelicera 21 Palp 22 Apotele.

Legs. Tarsi I–IV with claws and ambulacra (Figs 1, 10, 23–26). Leg chaetotaxy as follows: leg I: (Fig. 23) coxa 0 0/1 0/1 0, trochanter 1 1/2 0/1 1, femur 2 3/2 2/2 2, genu 2 3/2 3/1 2, tibia 2 3/2 3/1 2. Leg II: (Fig. 24) coxa 0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 2 3/1 2/2 1, genu 2 3/1 2/1 2, tibia 2 2/1 2/1 2, tarsus 3,3/2,3/2,3 + mv, md.Leg III: (Fig. 25) coxa 0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 1 2/0 1/1 1, genu 2 2/1 2/ 1 1, tibia 2 1/1 2/1 1, tarsus 3 3/2 3/2 3 + mv, md.Leg IV: (Fig. 26) coxa 0 0/1 0/0 0, trochanter 1 0/1 0/2 1, femur 0 2/1 1/1 1, genu 2 2/1 3/1 1 (Figs 6, 10, 26), tibia 2 1/1 3/1 2, tarsus 3 3/2 3/2 3 + mv, md.

Figures 23–26.

Pogonolaelaps beaulieui Nemati & Gwiazdowicz, gen. n., sp. n. (Female): 23 Leg I 24 Leg II 25 Leg III 26 Leg IV.

Figures 27–29.

Pogonolaelaps beaulieui Nemati & Gwiazdowicz gen. n., sp. n. (Male): 27 Ventral idiosoma 28 Chelicera 29 Femur II (lateral view).

Etymology

The name of the new genus is derived from the Greek word pogon (beard), and refers to the unusually long internal malae (gender masculine).

Note on subfamily placement of new genus

Evans and Till (1966) proposed six subfamilies for laelapid mites. We consider our new genus as a member of Laelapinae subfamily (sensuEvans and Till 1966) based on the presence of attributes of the subfamily: presternal area in the female reticulated or with pre-endopodal shields, deutosternum with five to seven (usually six) transverse rows of denticles, chelicerae in the female chelate-dentate or rarely chelate-edentate, pilus dentilis present, in the male chelate-dentate with spermadactyl free anteriorly or partially fused with the movable digit, hypertrichy of dorsal shield when present usually restricted to the region of the J series of setae, male with holoventral shield or with discrete sternito-genito-ventral and anal shields.

This subfamily contains seven genera: Ololaelaps, Androlaelaps, Ondatralaelaps, Laelaps, Hyperlaelaps, Pseudoparasitus and Hypoaspis sensu lato with nine subgenera for the latter like: Hypoaspis (Hypoaspis), H. (Alloparasitus), H. (Stratiolaelaps), H. (Cosmolaelaps), H. (Penumolaelaps), H. (Gymnolaelaps), H. (Holostaspis), H. (Laelaspis) and H. (Gaeolaelaps), which are considered as full generic status presently (Trägårdh 1952, Hunter 1966, Lindquist et al. 2009, Rosario 1981, Beaulieu 2009, Joharchi et al. 2011).

Remarks

Members of Pogonolaelaps gen. n. in general appearance may be similar to those genera of Laelapinaesensu Evans & Till (1966), which possess a broad epigynal shield such as Laelaspis, Gymnolaelaps, Pseudoparasitus, and due to the absence of st4 and presence of hypertrichy on dorsal shield to Laelaspisella (was considered in this subfamily by Marais and Loots 1969) and for its special shape of dorsal setae with small knob at their base to Cosmolaelaps genera.

Pogonolaelaps may be similar to Laelaspis but it can be distinguished readily by the following characters. In Laelaspis palptarsal claw is two-tined (three tined in Pogonolaelaps); genital shield in Laelaspis with characteristic ornamentation including two distinct Λ-shaped lines; with two pairs of setae on extreme edges of shield in addition to st5 (in Pogonolaelaps without this special ornamentation and with only one pair of setae on the shield margin); Laelaspis usually lacks pre-sternal shield (present in Pogonolaelaps, but not markedly sclerotized). Laelaspis genu IV with nine setae including one ventral seta (Pogonolaelaps with ten setae including two ventral setae: av and pv); male of Laelaspis with holoventral shield (Pogonolaelaps with separate sub-triangular anal shield); dorsal shield in Laelaspis lacks hypertrichy (Pogonolaelaps with hypertrichy on opisthonotal part); Laelaspis members with st4 and pore-like iv3 on integument posterior to sternal shield (Pogonolaelaps lacks st4 and iv3 located on posterolateral extension of sternal shield near st3).

Pseudoparasitus and Gymnolaelaps members have st4 and pore-like iv3 on integument posterior to sternal shield, conspicuous denticulate epistome, genu IV with one ventral seta (av), and holotrichous dorsal shield with acicular setae; known males have holoventral shield and lack large spine or spur-like setae on femur II. Pseudoparasitushas large genital shield with 4–5 pairs of setae including two pairs on the shield surface; however, all of those in Gymnolaelaps are located on the lateral margin of shield. Pogonolaelaps lacks st4 and iv3 located on posterolateral extension of sternal shield near st3, with smooth sub-triangular epistome, large genital shield with only one pair of setae (st5) on the lateral margin, genu IV with two ventral seta (av and pv), dorsal shield possesses setae with small knob at their base, opisthonotal part with hypertrichous condition, males have separate anal shield and have large spine-like seta (pv) on femur II.

Laelaspisella (L. macrodorsalis and L. epigynialis) (Marais and Loots 1969) has dorsal shield hypertrichous for both the podonotal and opisthonotal parts, and with very small acicular setae, setae Jv5 and Zv5 are spatulate and pilose, chelicera lacks dorsal seta, the setation of genu I is deficient in one postero-dorsal seta (2 3/2 2/1 2), while Pogonolaelaps has dorsal setae with small knob at their base, hypertrichous condition only of opisthonotal region, setae Jv5 and Zv5 acicular, chelicerae possess dorsal setae; the setation of genu I is typical with three pd setae (2 3/2 3/1 2). Differences among Pogonolaelaps gen. n. and other related genera such as Laelaspis, Gymnolaelaps, Pseudoparasitus, Laelaspisella and Cosmolaelaps are mentioned in Table 1.

Table 1.

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Comparison among Pogonolaelaps gen. n. and other related laelapid genera.

	Laelaspis	Gymnolaelaps	Pseudoparasitus	Laelaspisella	Cosmolaelaps	Pogonolaelaps
Type species	Laelaps astronomicus Koch,1839	Laelaps myrmecophilus Berlese, 1892	Laelaps meridionalis G & R Canestrini, 1882	Laelaspisella epigynialis Marais & Loots, 1969	Laelaps claviger Berlese, 1883	Laelaps canestrinii Berlese, 1903
Palp tarsal claw	two-tined	three tined, third tine occasionally reduced, rarely absent	three-tined, posterior tine small	two-tined	two-tined	three-tined
Two distinct Λ-shaped lines on genital shield	present	absent	absent	absent	absent	absent
Posterior margin of genital shield	rounded	straight	straight	nearly rounded or tapered	usually rounded	rounded
Setae on genital shield	all on edges of shield	all on edges of shield	at least two pairs well inside edges of shield	with only st5 on the margin of shield	with only st5 on the margin of shield	all on edges of shield
Pre-sternal plates	absent	present	present	present	usually present	present and not so discernable
Podal plates behind coxae IV	present	usually present	present	medium size, rounded	absent	medium size, rounded
Epistome	nearly always smooth	denticulate	denticulate	smooth	denticulate	smooth
Hypertrichy of dorsal shield	absent	absent	absent	present on whole dorsum	absent	present only on opisthonotal part
Dorsal shield setae	nearly always scimitar-shaped with small knob at their bases	acicular	acicular	acicular	with different shaped but never acicular	with small knob at their bases
Setae av and pv on genu IV	only with av	only with av	only with av	with av and pv	only with av	with av and pv
*Seta pd3* on tibia I**	present	present	present	absent	present	present
Male holoventral shield	present	present	present	?	nearly always present	absent, with separate sub-triangular anal shield
Large spine or spur-like seta(e) on male leg II	absent	absent	absent	?	absent	present
Seta st4	present	present	present	absent	present	absent
Spatulate elongate Jv5 and Zv5	absent	absent	absent	present	absent	absent
Cheliceral dorsal seta	present	present	present	absent	present	present
Internal malae with elongate densely hairs	absent	absent	absent	present	absent	present

Pogonolaelaps canestrinii (Berlese, 1903), comb. n.

Figures 1–6, 7–10, 11–14

Laelaps canestrinii Berlese, 1892: LXIX, 1.

Laelaps (Eulaelaps) canestrinii Berlese, 1903: 13.

Hypoaspis canestrinii. — Oudemans 1902: 24; Oudemans 1903; 129; Buitendijk 1945: 295.

Laelaspis (Hypoaspis) canestrinii. — Berlese 1904.

Gymnolaelaps canestrinii. — Vitzthum 1929: 25; Sellnick 1931: 695; Costa 1962: 491: Costa 1966: 74; Bhattacharyya 1968: 539; Joharchi et al. 2011: 23.

Laelaps canestrinii. — Tipton 1960: 290.

Hypoaspis (Gymnolaelaps) canestrinii. — Hunter 1967: 99; Bregetova 1977: 523.

Pseudoparasitus (Gymnolaelaps) canestrinii. — Karg 1981: 218; Karg 1989, 334; Karg 1993: 135.

Hypoaspis (Cosmolaelaps) canestrinii. — Karg 1979: 71.

Laelaspisella canestrinii. — Joharchi and Halliday 2013: 46; Moreira 2014: 316.

Specimens examined

Khuzestan province: Ahwaz (31°19'22"N, 48°40'50"E, H: 16 m), nest of unknown ant, two females, coll. A. Nemati, 2012; Baghmalek (31°31'22"N, 49°53'8"E, H: 707m), soil, one female and one male, coll. A. Nemati, 2012; Ghaletol (31°37'55"N, 49°53'20"E, H: 885 m), soil and nest materials of unknown ant, one female, coll. A. Nemati, 2012; Izeh (31°49'52"N, 49°52'9"E, H: 845 m), soil, two females, coll. A. Nemati, 2012 and Masjed-Soleiman (31°56'11"N, 49°18'14"E, H: 251 m), soil, one female, coll. A. Nemati, 2011. Chaharmahal Va Bakhtiari province: Shahrekord (32°19'39"N, 50°51'35"E, H: 2206 m), soil, three females, coll. A. Nemati, 2009, Lordegan (31°30'30"N, 50°49'39"E, H: 1594 m), soil, three females and two males, coll. A. Nemati, 2012; Naghan (31°56'19"N, 50°44'54"E, H: 2219 m), soil, one female, coll. M. Mohseni, 2010. Ben (32°32'32"N, 50°43'48"E, H: 2203 m), soil, four females and one male, coll. A. Nemati, 2011. Esfahan province: Esfahan (32°39'37"N, 51°41'22"E, H: 1608 m), soil, coll. A. Nemati, 2011. Kashan, soil, five females, coll. M. Fahiminezhad, 2006. Shahreza, soil, one female (32°07'N, 51°55'E, alt. 1725 m), 22 August 2010; one female (32°01'N, 51°53'E, alt. 1800 m), 20 March 2011; one female (32°01'N, 51°53'E, alt. 1806 m), 4 April 2011; three females (32°02'N, 51°51'E, alt. 1827 m), 11 June 2011; five females and two males (31°39'N, 51°55'E, alt. 2220 m), 9 July 2011; one female and one male (32°00'20"N, 51°52'54"E, alt. 1823 m), 17 July 2011; two females (31°56'N, 51°44'E, alt. 1963 m), 4 August 2011. Microslides were deposited in APAS.

Explanation concerning Berlese’ specimens were cited in the following text under notes on the male of Pogonolaelaps canestrinii.

Diagnosis

Podonotal region with 23 pairs of thin small setae with small knobs at their base (except for j1 and z1); opisthonotal region with 32 pairs of setae, three unpaired setae between J series and seven pairs of long barbed setae; pre-sternal area with a pair of indistinct poorly sclerotized plates; iv1-3 slit-like and located on the sternal shield surface; peritremes long, extending to coxa I anteriorly; internal malae densely fringed. Epistome with smooth anterior margin. Palp apotele three-tined; genu IV with 10 setae, including two ventral setae (av and pv).

Description of the female

(n = 7). Figures 1–10.

Dorsal idiosoma. Dorsal shield oval-shaped, length 624–723, width at level of setae r3 425-465; reticulation more distinct on opisthonotal part; podonotal region with 23 pairs of thin small setae with small knobs at their base (except for j1 and z1); opisthonotal region with 32 pairs of setae, three unpaired setae between J series and seven pairs of long barbed setae (Fig. 5). Dorsal setae short (26–36) except for longer setae on opisthonotal part (52–83). Dorsal idiosoma with 19 pairs of lyrifissures and pore-like structures.

Ventral idiosoma (Figs 1–4). Tritosternum with tubular base (39–47) and pilose laciniae (65–80). Pre-sternal area with a pair of indistinct poorly sclerotized plates (Figs 1, 2). Sternal shield (Figs 1, 2) reticulate anteriorly and laterally, smooth posteriorly, 80–96 long, 107–122 wide, anterior margin sinuate, convex medially, posterior margin deeply concave, sternal setae smooth, st1 (46–67), st2 (47–50), st3 (44–57), iv1 slit-like, located slightly anterior to st1; iv2 slit-like, between st2-st3, iv3 slit-like, located on postero-lateral corners of sternal shield; st4 absent. Genital shield (Figs 1, 3) broad, well ornamented, 346–374 long (including hyaline flap at the base of posterior margin of sternal shield), 177–195 wide at level of st5 and widest (239–260) near setae Zv1, abutting anal shield, bearing one pair of setae (st5 44–49); paragenital pores (iv5) on soft integument posterior to genital setae. Anal shield (Fig. 4) sub-triangular, reticulated, 99–109 long, 177–195 wide, post-anal seta (43–45) slightly longer than para-anal setae (36–42). Cribrum extending laterally to level of post-anal seta. Opisthogastric surface with: one pair of elongate metapodal plates plus two pairs of platelets (Figs 1, 3); 10–11 pairs of setae, 36–49 μm long; and seven pairs of pore-like structures, plus one pair (gv3) on lateral margin of anal shield. Stigmata surrounded by narrow, pointed stigmatal plate, which extends posteriorly past level of mid-coxae IV. Peritremes long, extending to anterior of coxa I. Endopodal, podal and exopodal plates as in genus.

Gnathosoma. Hypostome (Fig. 7) with three pairs of similar smooth simple setae (h1:69–79, h2: 21–30, h3: 72–84) plus one pair of palpcoxal setae (40–47). Deutosternal groove with six rows of denticles; corniculi normal, horn-like, reaching mid-level of palp femur; internal malae densely fringed with very elongate hairs. Epistome (Fig. 8) with smooth anterior margin. Arthrodial processes of chelicerae (Fig. 9) developed, movable digit (47–49) with two teeth, middle article (174–187), fixed digit with six teeth, setaceous pilus dentilis small. Palp attributes as in the genus.

Legs. Tarsi I–IV with claws and ambulacra. Legs I (559–580) and IV (587–605), longer than legs II (429–450) and III (429–455). Genu IV (Figs 6, 10) with 10 setae, including two ventral setae (av and pv).

Description of the male

(n = 5). Figures 11–14.

Dorsal idiosoma (Fig. 11). Dorsal shield 512–540 long, 300–315 wide, dorsal chaetotaxy as for female, except setae that are slightly shorter.

Ventral idiosoma (Fig. 12). Presternal area with transverse lines, not well sclerotised; sternito-genito-ventral shield, 351–357 long, 213–234 wide, reticulated throughout, bearing eight pairs of simple pointed setae, st1-st5 (26–36), Zv1 and Jv1–2 (36–45); with separate reticulated anal shield, 60–75 long, 94–106 wide; without metapodal plates. Soft cuticle with ten pairs of pointed, barbed setae.

Gnathosoma. As in female (Fig. 13); chelicerae (Fig. 14) with middle segment (122–130), fixed digit (34–36) bearing two teeth. Pilus dentilis setiform. Movable digit (34) with one tooth; spermatodactyl (41–44).

Legs. Tarsi I-IV with claws and ambulacra. leg I (490–556), leg II (354–400), leg III (387–411), leg IV (569–577), legs I and IV longer than legs II and III. Structure and chaetotaxy as in female, except femur II, which bears a spine-like pv1 seta.

Notes on the male of Pogonolaelaps canestrinii (Berlese), comb. n

The described males of Gymnolaelaps have a holoventral shield. Some confusion about the state of the male ventral shields in Pogonolaelaps canestrinii (Berlese), comb. n. exist as Berlese (1892) originally showed the anal shield not separated, but later Berlese (1904) illustrated the species with a separate anal shield. Costa (1962) and Hunter (1967) regarded the species as having a separate anal shield, and here we confirm this by checking the type specimens of P. canestrinii comb. n. kindly examined by Dr. Roberto Nanelli, and the type information is as follows:

Slide 4 Myrm./45 labeled Laelaps canestrinii Berl., 1 female, type, nidi formiche, Portici; (nests of ant, Portici: a locality near the city of Naples, Italy); Slide 4 Myrm./46 labelled Laelaps canestrinii Berl., 1 female, nidi formiche, Portici; Slide 4 Myrm./47 labelled Laelaps canestrinii Berl., 3 females, type, nidi formiche, Portici; Slide 4 Myrm./48 labelled Laelaps canestrinii Berl., 1 male, type, nidi formiche, (without locality of collection) (perhaps Portici); Slide 221/31 labelled Hypoaspis (Gymnolaelaps) canestrinii Berl., female, Spalato, libero nell’humus; (free, vacant in humus); The specimens are in poor condition but it is possible to see that the female’s dorsal shield has short setae, plus seven long thickened posterior setae, very similar to that shown in Figures 1, 11, 12. The slides labelled Myrm.= associated with ant, myrmecophilous.

Berlese (1892) described female and male specimens of Laelaps canestrinii, and according to his figures the female possesses a very wide epigynal shield with four pairs of setae in addition to st5 and with straight posterior margin, lacks setae between the epigynal and anal shields, sternal shield only with two pairs of setae, lacks the metasternal plates and setae, the movable digit of the chelicera with three teeth, and male without separate anal shield. Berlese (1904) redescribed Laelaps (Hypoaspis) canestrinii as epigynal shield of the female posteriorly rounded, carries only st5, possess one pair of setae between the epigynal and anal shields. In the male, the anal shield is clearly separate from the genito-ventral shield. The examination of the type material of P. canestrinii and figures by Berlese (1904) confirm the identity of specimens redescribed in this study.

Pogonolaelaps beaulieui gen. n., sp. n.

http://zoobank.org/F2A5A9D9-2409-4AF1-ACE7-3572A4463DAF

Figures 15–17, 18–22, 23–26, 27–29

Specimens examined

Holotype, female, Chaharmahal va Bakhtiari province, Shahrekord (32°19'39"N, 50°51'35"E, H: 2206 m), soil, 2009; coll. A. Nemati; Chaharmahal va Bakhtiari province, Saamaan, Cham-Khalifeh (32°30'35"N, 50°52'12"E, H: 1875 m), Walnut rooting wood, 13 June 2012, three females, coll. A. Nemati; Saamaan (32°30'36"N, 50°53'13"E, H: 1873 m), rooting wood, three females and two males, 27 May 2012, coll. A. Nemati; Shahrekord, Shahrekord University, nest materials of unknown ant, one female and one male, 31 August 2007, coll. A. Nemati; Shahrekord, Shahrekord University, nest materials of unknown ant, two females, August 2006, coll. H. Maleki.

Diagnosis

iv3 slit-like located posterior to st3 on postero-lateral corners of sternal shield, with large crescent-shaped podal shield posterior to coxa IV; dorsal shield with 23 pairs of setae on podonotal (rx seta present on podonotal part), and 28 pairs on opisthonotal part, plus 1-3 unpaired setae between J series; 7–9 pairs of thickened, elongated, and barbed opisthonotal setae; genu I with normal chaetotaxy (2 3/2 3/1 2), and genu IV with ten setae including two ventral setae (av and pv).

Description of the female

(n = 7). Figures 15–26.

Dorsal idiosoma (Fig. 15). Dorsal shield length 728–780, width 517–560, oval shaped, wraps around and flaps onto the ventral idiosoma; reticulation more distinct on opisthonotal part, with 51 pairs of setae, 23 pairs on podonotal, 28 pairs on opisthonotal region, plus 1–3 Jx setae between J series (usually with three); setae increasing in length from anterior to posterior and from dorso-central to dorso-lateral part, dorso-central setae length on podonotal (23–42) and dorso-lateral setae (50–80), lateral opisthonotal setae tend to reach well past base of next posterior setae, lateral opisthonotal part with 7–9 pairs of long, thick, barbed setae (98–130), length of central opisthonotal setae 31–52, lateral opisthonotal setae 62–75. Dorsal setae scimitar-shaped with a small basal knob (Fig. 17). Dorsal shield with 19 pairs of pore-like structures, nine pairs on podonotal and ten pairs on opisthonotal (six pairs of those are large and slit-like) (Fig. 15).

Ventral idiosoma (Fig. 16a, b). Tritosternum with columnar base (19–26) and paired pilose laciniae (85–93); pre-sternal plates weakly sclerotized and ornamented with transverse lines. Sternal shield with reticulate anterior and lateral margins, smooth posteriorly, 86–94 long, narrowest between coxae II (146–156), widest between coxae II and III (213–221), anterior margin sinuate, convex medially, posterior margin deeply concave. Sternal setae smooth, st1 (75–83), st2 (62–70) and st3 (62–78), iv1 slit-like, located slightly anterior to st1; iv2 slit-like, between st2 and st3, iv3 slit-like located posterior to st3, on postero-lateral corners of sternal shield; st4 absent. Endopodal plates II/III fused to lateral margins of sternal shield, endopodal plates III/IV elongate, curved. Genital shield broad, 377–395 long (including hyaline flap at the base of posterior margin of sternal shield), 208–226 wide at level of st5 and widest (260–273) near setae Zv1, abutting anal shield, well ornamented, with one pair of setae (st5 = 73–75) on shield and three pairs of setae adjacent to lateral edges; circular paragenital pores (iv5) located on soft integument between coxa IV and pair of minute narrow platelets. Anal shield subtriangular, 125–133 long, 151–156 wide, reticulated, post-anal seta (65–75) slightly longer than para-anal setae (52–60). Cribrum in a strip form of teeth, extending antero-laterally to level of post-anal seta. Opisthogastric surface with: one pair of elongate metapodal plates, two pairs of minute platelets (Fig. 16a), 12 pairs of long setae (Figs 16a,b) (Jv1 91–93, Jv2 83–88, Jv3 78–86, Jv4–5 98–104, Zv1 96–99, Zv2–3 88, Zv4–5 98–104), and four pairs of pore-like structures, plus one pair of adanal gland pores gv3 on lateral margin of anal shield (Figs 16a, b). Endopodal, podal and exopodal plates are as in genus. Peritreme extending from coxa IV to anterior level of coxa I, peritrematal shield wide, with two pairs of post-stigmatal pores, one pair of small pores anterior to stigmata and two pairs of pores (ip, gp) at level of coxae II/III.

Gnathosoma. Hypostome with three pairs of smooth simple setae: h1, h3 (70–75), h2 (23–26). Palpcoxal setae 36–39. Deutosternal groove with six rows of denticles, each bearing 7–9 small teeth except first row with three larger teeth; corniculi normal, horn-like, reaching beyond of mid-level of palp femur; internal malae free medially and densely fringed with elongate threads (Fig. 18). Epistome sub-triangular with smooth antero-lateral margins (Fig. 19). Chelicera with dorsal seta, lyrifissure and developed arthrodial processes (Fig. 20), movable digit (44) with two teeth, middle article (182–190), fixed digit with two moderately large teeth proximal to pilus dentilis, followed by four minute teeth and a small offset tooth subapically, setaceous pilus dentilis moderately robust. Palp chaetotaxy normal (sensuEvans and Till 1965) and as in genus, with simple setae except al on femur, and al2 of genu slightly thickened (Fig. 21), palp apotele three-tined (Figs 21, 22).

Legs. Tarsi I–IV with claws and ambulacra. Legs I (595–647) and IV (699–704), longer than legs II (455–486) and III (478–509) (excluding pre-tarsus). Leg chaetotaxy as follows: leg I: (Fig. 23) coxa 0 0/1 0/1 0, trochanter 1 1/2 0/1 1, femur 2 3/2 2/2 2, genu 2 3/2 3/1 2, tibia 2 3/2 3/1 2. Leg II: (Fig. 24) coxa 0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 2 3/1 2/2 1, genu 2 3/1 2/1 2, tibia 2 2/1 2/1 2, tarsus 3,3/2,3/2,3 + mv, md.Leg III: (Fig. 25) coxa 0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 1 2/0 1/1 1, genu 2 2/1 2/ 1 1, tibia 2 1/1 2/1 1, tarsus 3 3/2 3/2 3 + mv, md. Leg IV: (Fig. 26) coxa 0 0/1 0/0 0, trochanter 1 0/1 0/2 1, femur 0 2/1 1/1 1, genu 2 2/1 3/1 1, tibia 2 1/1 3/1 2, tarsus 3 3/2 3/2 3 + mv, md.

Insemination structures. Not seen, apparently unsclerotised.

Description of the male

(n = 3). Figures 27–29.

Dorsal idiosoma. Dorsal shield length 585–606 long, width 457–470, dorsal chaetotaxy as for female, except setae which are slightly shorter.

Ventral idiosoma (Fig. 27). With weakly sclerotised presternal shields; sterniti-genito-ventral shield, 413–420 long, 247–257 wide, reticulated throughout, bearing eight pairs of simple, pointed setae, st1-st5 (38–42), Zv1 and Jv1–2 (58–63); with separate reticulated anal shield, 99–105 long, 110–122 wide, post-anal seta (55–59) longer than para-anals (40–44); without metapodal plates, apparently fused to the lateral margin of sterniti-genito-ventral shield. Soft cuticle with eight pairs of pointed, mostly barbed setae.

Gnathosoma. As in female; chelicerae (Fig. 28) with middle segment (133–141), fixed digit (38–40) bearing two teeth. Pilus dentilis setiform. Movable digit (35–37) with one tooth; spermatodactyl (45) relatively straight.

Legs. Tarsi I-IV with claws and ambulacra. Leg I (510–525), leg II (390–401), leg III (438–445), leg IV (582–603), legs I and IV longer than legs II and III. Structure and chaetotaxy as in female, except for femur II, which bears a spine-like and thickened pv1 seta (Fig. 29).

Etymology

This species is named in honor of Dr. Frederic Beaulieu (Canadian National Collection of Insects, Arachnids and Nematodes, Agriculture and Agri-Food Canada, Ottawa, Canada).

Remarks

Pogonolaelaps beaulieui gen. n., sp. n. is similar to P. canestrinii comb. n. and can be readily distinguished from it by the presence of 28 pairs of setae on opisthonotal region plus 1–3 unpaired setae (32 pairs in P. canestrinii plus 3 unpaired setae between J series); the dorsal setae of P. beaulieui gen. n., sp. n. are much longer than those of P. canestrinii comb. n. (see text), and the genital shield in P. beaulieui gen. n., sp. n. [377–395 long, 208–226 wide at level of st5 and widest (260–273) near setae Zv1] is longer and wider than that of P. canestrinii comb. n. [346-374 long, and 177-195 wide at level of st5 and widest (239-260 μm) near setae Zv1].

Discussion

Karg (1989) considered Gymnolaelaps as a subgenus of Pseudoparasitus based on the presence of a three-tined apotele, developed podal shields posterior to coxa IV, epistome with denticulate anterior margin and the presence of 1-3 pairs of setae on lateral edges of an expanded genital shield. Other authors defined Gymnolaelaps at the genus level, with diagnoses provided by Hunter (1967), Hunter and Costa (1971) and, most recently, Joharchi et al. 2011 and Joharchi and Halliday 2013. According to these last authors the genus is defined by: genu IV with nine setae including one ventral seta; the metasternal setae always present; dorsal shield covering dorsum and even extending ventrally, has a normal complement of 40 pairs of setae, often with paired Zx setae between J and Z setae, unpaired Jx setae also often present; dorsal shield setae distally pointed, smooth or slightly serrated, not long and whip-like; and Zv1, Jv1-2 setae (additional to st5) are on the margin of the shield. The only character that separates Gymnolaelaps from Pseudoparasitus seems to be the given: in Pseudoparasitus, at least two pairs of ventro-genital setae are set well inside the edges of the shield (Joharchi et al. 2011, Joharchi and Halliday 2013).

The generic definition of Gymnolaelaps does not apply for all species that are assigned to this genus nowadays, as there are several characters not taken into account and excluded by the diagnosis of Joharchi et al. (2011) andJoharchi and Halliday (2013). These exceptions occur in the genito-ventral shield of female, the number of the apotele tines, the form of the anterior margin of the epistome, leg and dorsal chaetotaxy, and the form of the podal shield posterior to coxa IV.

Gymnolaelaps shealsi Hunter & Costa, 1971 has a genito-ventral shield that does not extend to the anal shied, lacks an expanded podal plate behind coxa IV and the epistome is triangular with smooth margins. Gymnolaelaps viennensis Sellnick is similar to G. shealsi in the shape of genital shield. Gymnolaelaps krantzi (Hunter, 1967) has a two-tined apotele and the epistome has a smooth rounded anterior margin. Gymnolaelaps unospinosus (Karg, 1978) has thickened seta on femur II (not included in the diagnosis of Joharchi et al. 2011 and Joharchi and Halliday 2013) and has a very narrow podal plate behind coxa IV.

Notes on Pseudoparasitus reniculus Karg, 1981 and P. triquetrus Karg, 2003

Pseudoparasitus (Gymnolaelaps) reniculus Karg, 1981 has four pairs of setae on genital shield, of which Jv1-2 inserted away from the shield margins and lacks the holotrichous condition on dorsal shield (slightly hypertrichous) on the opisthonotal part. According to our definition of Gymnolaelaps and the difference between Gymnolaelaps and Pseudoparasitus genera, we consider this species in its original genus Pseudoparasitus not in Gymnolaelaps as proposed by Joharchi et al. (2011) and Moreira (2014).

Pseudoparasitus (Gymnolaelaps) triquetrus was described by Karg (2003) in Ecuador, as a species in Gymnolaelaps (as subgenus) in which the genital shield expands behind coxa IV and Zv1, Jv1-2 located on the surface of genital shield far from the edges. Based on these characters, it is also considered as a species in its original genus Pseudoparasitus and not in Gymnolaelaps as proposed by Moreira (2014).

Notes on Laelaspisella Marais & Loots

Laelaspisella was originally described by Marais and Loots (1969) by discussing several morphological characters (Marais and Loots 1969). Joharchi and Halliday (2013) have defined Laelaspisella by considering three main characters: dorsal shield hypertrichy; absence of metasternal setae and genu IV bearing two ventral setae; however none of those are apomorphic characters for this genus. In addition to Laelaspisella macrodorsalis Marais & Loots, and L. epigynialis Marais & Loots, Gymnolaelaps canestrinii and G. kabitae were also transferred to Laelaspisella (Joharchi and Halliday 2013).

The new species here (P. beaulieui), has hypertrichous dorsal shield (but in opisthonotal part), absent metasternal setae and genu IV with two ventral setae, which in accordance with the idea of Joharchi and Halliday (2013) puts it in genus Laelaspisella. However when considering some other characters within genus mentioned above, several problems would arise assigning this new species and P. canestrinii to Laelaspisella. Herein we discussed it below.

First, Laelaspisella (L. macrodorsalis and L. epigynialis) (Marais and Loots 1969) has ovoid pre-endopodal plates reticulated and well sclerotised but in P. canestrinii and P. beaulieui gen. n., sp. n. the pre-endopodal plates are not so sclerotized. In Gymnolaelaps the posterior half of the pre-endopodal plate is usually strongly sclerotized, and the anterior half is less sclerotized. The pre-endopodal plates of L. macrodorsalis, and L. epigynialis are not described in sufficient detail, but according to the illustrations these ovoid plates are conspicuous and with line ornamentations.

Second, the sternal shield of Laelaspisella has two pairs of poroids or lyrifissures, iv1 and iv2. The metasternal pores iv3 apparently are absent (Marais and Loots 1969). Pogonolaelaps canestrinii comb. n. and P. beaulieui gen. n., sp. n., have slit-like iv3 on the surface of postero-lateral corners of sternal shield. However Marais and Loots didn’t mention about iv3 in Laelaspisella, but according to their illustrations iv3 are not present in L. macrodorsalis and L. epigynialis. Species of Gymnolaelaps have poroid iv3 posterior to sternal shield on soft integument.

Third, Joharchi and Halliday (2013) have cited the absence of st4 as one of the three main characters of Laelaspisella genus (L. macrodorsalis and L. epigynialis), but metasternal setae are also absent in some other taxa like genus Reticulolaelaps Costa, 1968 and some species of Hypoaspissens. lat., such as Hypoaspis (Hypoaspis) metapodalis Karg, 1978; H. (H.) eugenitalis Karg, 1978; H. (Alloparasitus) pycnosis Karg, 1982 and H. (Holostaspis) tridentata Karg, 1982 (These scientific names are mentioned here as in the related literature). This character could not be considered as apomorphic for Laelaspisella due to the presence in different genera and species. Pogonolaelaps beaulieui gen. n., sp. n. and P. canestrinii comb. n. lack st4.

Fourth, the genito-ventral shield in Laelaspisella (L. macrodorsalis and L. epigynialis) (Marais and Loots 1969) is not reaching the anal shield and there are two pairs of setae between genito-ventral and anal shields. This shield has one pair of setae (st5), widened slightly behind the genital setae and is rounded or tapered posteriorly. This condition was observed in L. macrodorsalis (posterior edge distinctly tapered), L. epigynialis (posterior edge rounded or slightly tapered), but P. canestrinii comb. n. and P. beaulieui gen. n., sp. n. have genital shield broad, strongly widened posterior to genital setae, posterior edge rounded, and abutting anal shield. Such condition can be seen in some species of Gymnolaelaps.

Fifth, setae Jv5 and Zv5 are spatulate and pilose in Laelaspisella (L. macrodorsalis and L. epigynialis) (Marais and Loots 1969) and it is suspected to represent an apomorphic character. Setae Jv5 and Zv5 are acicular in P. canestrinii comb. n., and P. beaulieui gen. n., sp. n. (with small barbs), and all known species of Gymnolaelaps.

Sixth, in species of Laelaspisella (L. macrodorsalis and L. epigynialis), and some species of Gymnolaelaps like, G. krantzi (Hunter) and G. obscuroides (Costa) the palp apotele has two tines. In Gymnolaelaps, there is a variation of the palp apotele and most species have the apotele 3-tined, but in a few species the third tine is reduced or lost. Maybe this represents a secondary loss of the third tine in some species. In P. canestrinii, and P. beaulieui gen. n., sp. n. it is 3-tined.

Seventh, Laelaspisella species (L. macrodorsalis and L. epigynialis) lacks dorsal seta of chelicerae, but Gymnolaelaps members and also P. beaulieui gen. n., sp. n. and P. canestrinii have this seta.

Eighth, the anterior margin of the epistome is smooth and sharply mucronated in L. macrodorsalis and L. epigynialis. In P. canestrinii, and P. beaulieui gen. n., sp. n. the anterior margin is smooth and pointed but not as sharply mucronate as in former. The most species of Gymnolaelaps have denticulate epistome.

Ninth, species of Laelaspisella (L. macrodorsalis and L. epigynialis) have the dorsal shield hypertrichous in both the podonotal and opisthonotal region with very small and acicular setae. Hypertrichous condition can be seen in the other laelapid mites like genus Reticulolaelaps (on whole dorsal shield), some species of Pneumolaelaps, and Gaeolaelaps ciconia (Karg, 1982): with this character in opisthonotal and opisthogastric regions, Gaeolaelaps ardoris (Karg, 1993): on both podonotal and opisthonotal parts. In P. canestrinii, and P. beaulieui gen. n., sp. n. dorsal shield is hypertrichous only in the opisthonotal region and dorsal setae possess small knob at their base. The podonotal region with holotrichous situation and rx located on the shield. Gymnolaelaps species have holotrichous condition with acicular setae pointed distally, and lack rx on the shield.

Tenth, in Laelaspisella species (L. macrodorsalis and L. epigynialis) the setation of genu I is deficient in one postero-dorsal seta (2 3/2 2/1 2). According to the Evans and Till (1965) and Lee (1970) it can be stated that the chaetotaxy of genu I is more stable than genu IV in mesostigmatid mites. Evans and Till (1965) noted some exceptions to the normal chaetotaxy of genu I (2 3/2 3/1 2) in some taxa of Dermanyssoidea such as Pseudolaelaps doderoi, Dermanyssus spp. and Ornithonyssus spp., that have been excluded from Laelapidae and at present are members of Pachylaelapidae, Dermanyssidae and Macronyssidae, respectively (Evans and Till 1965, Lindquist et al. 2009). For this reason, genu I in laelapid mites has normal chaetotaxy. Laelaspisella (L. macrodorsalis and L. epigynialis) has unique chaetotaxy in genu I with only two postero-dorsal setae (2 3/2 2/1 2), so this can be considered as an apomorphic character for Laelaspisella (L. macrodorsalis and L. epigynialis). Gymnolaelaps species, as most other free-living laelapids, have three postero-dorsal setae (2 3/2 3/1 2) on genu I. Pogonolaelaps beaulieui gen. n., sp. n. and P. canestrinii are no exception with a typical set of setae.

Eleventh, in Laelaspisella species (L. macrodorsalis and L. epigynialis) the chaetotaxy of genu IV deviates from the normal (2 2/1 3/0 1) due to the presence of a postero-ventral seta (2 2/1 3/1 1).

Joharchi and Halliday (2013) emphasized the presence of two ventral setae on genu IV as one of the three main characters of Laelaspisella species (L. macrodorsalis and L. epigynialis). The greatest diversity of leg segments chaetotaxy in laelapid mites has been observed in genu IV. In addition to normal pattern (2 2/1 3/0 1), three other types of chaetotaxy have been observed (Evans and Till 1965): in Laelaps agilis (2 2/1 3/1 2); L. echidninus, L. hilaris, L. muris, Hyperlaelaps amphibian, Hyperlaelaps microti, Eulaelaps stabularis, Eulaelaps nova, Haemolaelaps casalis, H. fahrenholzi, Euandrolaelaps sardoa and Laelaspulus flexuosus (2 2/1 3/0 2); Pneumolaelaps genus, and Melittiphis alvearius (2 2/1 3/1 1). This means that there are some genera in laelapid mites that have two kinds of genu IV chaetotaxy with 9 or 10 setae, including one or two ventral setae. Pogonolaelaps canestrinii, and P. beaulieui gen. n., sp. n. have two ventral setae on genu IV. Gymnolaelaps has one ventral seta on genu IV.

Laelaspisella can be defined as laelapid mites with the following characters:

There are two pairs of pores on the sternal shield, iv3 apparently absent; the metasternal setae are absent; the genital shield slightly widened behind the genital setae and is rounded or somewhat tapered posteriorly, but never touching the anal shield; setae Jv5 and Zv5 are spatulated and pilose; the palptarsal claw two tined; chelicerae lacks dorsal seta; the anterior margin of the epistome is smooth and sharply mucronated; the whole dorsal shield with hypertrichous condition; the setation of genu I is deficient for one postero-dorsal seta (2 3/2 2/1 2); the chaetotaxy of genu IV deviates from the normal (2 2/1 3/0 1) due to the presence of a postro-ventral seta (2 2/1 3/1 1). Finally, according to the explanations above the genus Laelaspisella comprises two species L. macrodorsalis and L. epigynialis at present.

Notes

Gymnolaelaps tonsilis (Karg, 1989)

Pseudoparasitus (Gymnolaelaps) tonsilis Karg, 1989: 335.

Gymnolaelaps tonsilis. — Farrier and Hennessey 1993: 74; Moreira 2014: 281.

Specimens examined

Pseudoparasitus tonsilis Karg, 1989, Chel. Nr. 3947♂, paratypus, ZMB Kat. Nr. 41478, St. Lucia, Antillen, Gastries, Vigie, Point Eins.: Dr. Mahunka, Budapest, 21 .7.80; Chel. Nr. 3944♀, ZMB Kat. Nr. 41475 (paratypes); Nr. 3945♀, ZMB Kat. Nr. 41476 (paratypes); Nr. 4440 ♀, ZMB Kat. Nr. 42589 (holotypus); Nr. 3943♀, ZMB Kat. Nr. 41474 (paratypes); Nr. 3946♂, ZMB Kat. Nr. 41477 (paratypes): with the same data as above on 11.7.80.

Gymnolaelaps kabitae Bhattacharyya, 1968

Gymnolaelaps kabitae Bhattacharyya, 1968: 537.

Pseudoparasitus (Gymnolaelaps) kabitae. — Karg 1989: 334.

Laelaspisella kabitae. — Joharchi and Halliday 2013: 47; Moreira 2014: 317.

Specimens examined

Ghaletol, Khuzestan province, nest materials of unknown ant, two females and two males, 2012-2013, coll. A. Nemati; Shahrekord, Chaharmahal va Bakhtiari province, nest materials of unknown ant, one female, 2014, coll. A. Nemati; Izeh,, Khuzestan province, nest of Pheidole pallidula (Hym., Formicidae), two females, one male, coll. A. Nemati. All specimens were deposited in APAS.

Psedoparasitus (Gymnolaelaps) tonsilis Karg, 1989 and Gymnolaelaps kabitae Bhattacharyya, 1968 possess denticulate epistome, two tined apotele, internal malae normal and lack very elongate setae and barbed, chelicera with dorsal seta, sternal shield with iv1-3, lack st4, genital shield rounded posteriorly and bear one pair of setae, ventral setae acicular, with hypertrichous condition in whole dorsal shield (based on personal observation of first author on type materials of G. tonsilis and in spite of its original description in Karg 1989) and with simple acicular setae, males with separate sternito-genital and anal shields and without spine like setae on leg II, genu IV with two ventral setae (av and pv), genu I with three pd setae (pd1-3). Those are differed from Laelaspisella by having denticulate epistome, presence of dorsal seta on fixed digit of chelicera, genu I with pd1-3, opisthogastric setae simple acicular, internal malae without elongate setae. These species also differed from Pogonolaelaps gen. n. by having simple acicular dorsal setae, hypertrichy on whole dorsal shield, denticulate epistome, two tined apotele, and the absence of spine like setae on leg II of male, internal malae without very elongate setae.

Acknowledgements

This research was supported by grants no. 89003738 sponsored by the Iran National Science Foundation (INSF). We sincerely thank to INSF for their financial support. We are very grateful to an anonymous reviewer and Dr. Farid Faraji for their constructive comments on earlier version of manuscript, to Dr. Roberto Nanelli (Italy) for his valuable help with specimens of Pogonolaelaps canestrinii in Berlese’s collection, to Dr. Fred Beaulieu and Dr. Bruce Halliday for their valuable comments and advice on Laelaspisella and Gymnolaelaps genera. We are very grateful to Elham Riahi, Esmaeil Babaeian, Mastaneh Mohseni and Mohammad Ameri for their helping out in some figures preparation. We also thank Arsalan Khalili-Moghadam, Mahdiyeh Fahiminezhad and Hosein Maleki (Shahrekord University, Shahrekord) for helping the first author during field sampling. Our gratitude goes to Dr. Jason A. Dunlop (Museum für Naturkude, Berlin, Germany) providing facilities to study the type materials of some laelapid mites.

References

Baker EW, Wharton GW (1952) An Introduction to Acarology. Macmillan Co., New York.

Beaulieu F (2009) Review of the mite genus Gaeolaelaps Evans and Till (Acari: Laelapidae), and description of a new species from North America, G. gillespiei n. sp. Zootaxa 2158: 33–49.

Berlese A (1892) Acari, Myriopoda et Scorpiones hucusque in Italia reperta 69, 22 pp.

Berlese A (1903) Diagnosi di alcuni nuove specie di Acari italiani, mirmecofili e liberi. Zoologischer Anzeiger 27: 12–28.

Berlese A (1904) Illustrazione iconografica degli Acari mirmecofili. Redia 1: 299–474.

Berlese A (1916) Centuria seconda di Acari nuovi. Redia 12: 125–177.

Berlese A (1923) Centuria sesta di Acari nuovi. Redia 15: 237–262.

Bhattacharyya SK (1968) Studies on Indian mites (Acarina: Mesostigmata). 6. Six records and descriptions of nine new species. Acarologia 10: 527–549.

Bregetova NG (1977) Family Laelaptidae Berlese, 1892. In: Ghilyarov MS, Bregetova NG (Eds) Key to the Soil Inhabiting Mites. Mesostigmata. Nauka, Leningrad, 483–554. [In Russian]

Buitendijk AM (1945) Voorloopige catalogus van de Acari in de collective Oudemans. Zoologische Mededelingen 24: 281–391.

Casanueva ME (1993) Phylogenetic studies of the Free-living and arthropod associated Laelapidae (Acari: Mesostigmata). Gayana Zoologia 57(1): 21–46.

Costa M (1962) Mites from the nests of the mole-rat (Spalax ehrenbergi) in Israel. Annals and Magazine of Natural History 13(4): 481–503. doi: 10.1080/00222936108651166

Costa M (1966) The present knowledge of mesostigmatic mites in Israel (Acari, Mesostigmata). Israel Journal of Zoology 15: 69–82.

Evans GO (1963a) Observations on the chaetotaxy of the legs in the free-living Gamasina (Acari: Mesostigmata). Bulletin of the British Museum (Natural History) Zoology 10(5): 277–303.

Evans GO (1963b) Some observations on the chaetotaxy of the pedipalps in the Mesostigmata (Acari). Annals and Magazine of Natural History 13(6): 513–527. doi: 10.1080/00222936308651393

Evans GO, Till WM (1965) Studies on the British Dermanyssidae (Acari: Mesostigmata) (Part 1) External morphology. Bulletin of the British Museum (Natural History) Zoology 13: 247–294.

Evans GO, Till WM (1966) Studies on the British Dermanyssidae (Acari: Mesostigmata). Part II. Classification. Bulletin of the British Museum (Natural History) Zoology 14: 107–370.

Evans GO, Till WM (1979) Mesostigmatic mites of Britain and Ireland (Chelicerata: Acari–Parasitiformes). An introduction to their external morphology and classification. Transactions of the Zoological Society of London 35: 145–270. doi: 10.1111/j.1096-3642.1979.tb00059.x

Faraji F, Halliday B (2009) Five new species of mites (Acari: Laelapidae) associated with large Australian cockroaches (Blattodea: Blaberidae). International Journal of Acarology 35(3): 245–264. doi: 10.1080/01647950903059445

Hunter PE (1966) The genus Pneumolaelaps with description of three new species (Acarina: Laelaptidae). Journal of Kansas Entomological Society 39(3): 357–369.

Hunter PE (1967) Comments on Hypoaspis (Gymnolaelaps) Berlese, 1916 with description of a new species (Acarina: Dermanyssidae; Laelapinae). Journal of the Georgia Entomological Society 2: 100–102.

Hunter PE, Costa M (1971) Description of Gymnolaelaps shealsi n. sp. (Acarina: Mesostigmata) associated with the imported fire ant. Journal of the Georgia Entomological Society 6(1): 51–53.

Joharchi O, Halliday B (2013) A new species and new records of Gymnolaelaps Berlese from Iran (Acari: Laelapidae), with a review of the species occurring in the Western Palearctic Region. Zootaxa 3646(1): 39–50. doi: 10.11646/zootaxa.3646.1.3

Joharchi O, Halliday B, Saboori A, Kamali K (2011) New species and new records of mites of the family Laelapidae (Acari: Mesostigmata) associated with ants in Iran. Zootaxa 2972: 22–36. doi: 10.11646/zootaxa.2883.1.2

Johnston DE, Moraza ML (1991) The idiosomal adenotaxy and poroidotaxy of Zerconidae (Mesostigmata: Zerconina). In: Dusbábek F, Bukva V (Eds) Modern Acarology. Academia, Prague, 349–356.

Karg W (1978) Die Gattung Pseudoparasitus Oudemans, 1902. Mitteilungen aus dem Zoologischen Museum in Berlin 54: 203–212. doi: 10.1002/mmnz.19780540202

Karg W (1979) Die Gattung Hypoaspis Canestrini, 1884 (Acarina, Parasitiformes). Zoologische Jahrbücher Abteilung für Systematik, Ökologie und Geographie der Tiere 106: 65–104.

Karg W (1981) Zur Kenntnis der Raubmilbengattung Pseudoparasitus Oudemans, 1902. Deutsche Entomologische Zeitschrift 28: 209–220. doi: 10.1002/mmnd.19810280402

Karg W (1982) Zur Kenntnis der Raubmilbeng Hypoaspis Canestrini 1884 (Acarina, Parasitiformes). Mitteilungen aus dem Zoologischen Museum in Berlin 58: 233–256.

Karg W (1989) Neue Raubmilbenarten der Gattung Pseudoparasitus Oudemans, 1902 (Acarina, Parasitiformes). Acarologia 30: 333–339.

Karg W (1993) Acari (Acarina), Milben. Parasitiformes (Anactinochaeta). Cohors Gamasina Leach. Raubmilben. 2. Überarbeitete Auflage. Die Tierwelt Deutschlands 59: 1–523.

Karg W (2000) Zur Systematik der Raubmilbenfamilien Hypoaspididae v. Vitzthum, 1941 und Rhodacaridae Oudemans, 1902 (Acarina, Parasitiformes) mit neuen Arten aus Süd– und Mittelamerika. Mitteilungen aus dem Museum für Naturkunde in Berlin, Zoologische Reihe 76: 243–262. doi: 10.1002/mmnz.20000760207

Karg W (2003) Neue Raubmilbenarten aus dem tropischen Regenwald von Ecuador mit einem kritischen Beitrag zur Merkmalsevolution bei Gamasina (Acarina, Parasitiformes). Mitteilungen aus dem Museum für Naturkunde in Berlin Zoologische Reihe 79: 229–251. doi: 10.1002/mmnz.20030790203

Krantz GW (1998) A new genus and two new species of hypoaspidine mites (Acari: Laelapidae) associated with Old World carpenter bees of the tribe Xylocopini (Hymenoptera: Apidae: Xylocopa). International Journal of Acarology 24: 291–300. doi: 10.1080/01647959808683595

Krantz GW, Ainscough BD (1990) Acarina: Mesostigmata (Gamasida). In: Dindal DL (Ed.) Soil Biology Guide. Wiley & Sons, New York, 583–631.

Lee DC (1970) Rhodacaridae (Acari: Mesostigmata); classification, external morphology and distribution of genera. Records of the South Australian Museum 16(3): 1–219.

Lindquist EE, Evans GO (1965) Taxonomic concepts in the Ascidae, with a modified setal nomenclature for the idiosoma of the Gamasina (Acarina: Mesostigmata). Memoirs of the Entomological Society of Canada 47: 1–64. doi: 10.4039/entm9747fv

Lindquist EE, Krantz GW, Walter DE (2009) Order Mesostigmata. In: Krantz GW, Walter DE (Eds) A Manual and Acarology, Third edition. Texas Tech University Press, Lubbock, 124–232.

Marais JF, Loots GC (1969) Laelaspisella, a new dermanyssid genus (Acari: Mesostigmata) from the Ethiopian Region. Wetenskaplike Bydraes van die P.U. vir C.H.O., Reeks B, Natuurwetenskappe 4: 1–10.

Moreira GF (2014) Taxonomic studies of laelapid mites (Acari: Mesostigmata: Laelapidae) and their use in combination with entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) to control Frankliniella occidentalis (Thysanoptera: Thripidae). PhD Thesis, Universidade Estadual Paulista- Unesp Campus de Jaboticabal, 522 pp.

Oudemans AC (1902) New list of Dutch Acari. Second Part. With remarks on known and descriptions of a new subfamily, new genera and species. Tijdschrift voor Entomologie 45: 1–70.

Oudemans AC (1903) Acarologische Aanteekeningen, VIII. Entomologische Berichten 14: 100–103.

Radovsky FJ, Gettinger D (1999) Acanthochelinae, new subfamily (Acari: Parasitiformes: Laelapidae), with redescription of Aconthochela chilensis Eving and description of a new genus and species from Argentina. International Journal of Acarology 25(2): 77–90. doi: 10.1080/01647959908683619

Rosario RMT (1981) Philippine Hypoaspidinae (Acarina: Mesostigmata: Laelapidae). Philippine Entomologist 5: 23–82.

Sellnick M (1931) XVI. Acari. Zoologische Forschungsreise nach den Jonischen Inseln und dem Peloponnes. Sitzungsberichte der Akademie der Wissenschaften in Wien. Mathematisch-Naturwissenschaftliche Klasse. ABT 1, Mineralogie, Biologie, Erdkunde 140: 693–776.

Sellnick M (1935) Milben-Neubeschreibungen. Zoologische Jahrbücher Abteilung für Systematik, Ökologie und Geographie der Tiere 66: 345–355.

Strong K, Halliday B (1994) Three New Species of Hypoaspis Canestrini (Acarina: Laelapidae) Associated with Large Australian Cockroaches. Journal of Australian Entomological Society 33: 87–96. doi: 10.1111/j.1440-6055.1994.tb00927.x

Tipton VJ (1960) The genus Laelaps with a review of the Laelapinae and a new subfamily Alphalaelaptinae (Acarina: Laelaptidae). University of California Publications in Entomology 16(6): 233–356.

Trägårdh I (1952) Acarina, collected by the Mangarevan expedition to South Eastern Polynesia in 1934 by the Bernice P. Bishop Museum, Honolulu, Hawaii. Mesostigmata. Arkiv för Zoologi 4: 45–90.

Van Aswegen PIM, Loots GC (1970) A taxonomic study of the genus Hypoaspis Canestrini sens. lat. (Acari: Laelapinae) in the Ethiopian region. Publicações Culturais da Companhia de Diamantes de Angola 82: 167–213.

Vitzthum HG (1929) 5. Ordnung: Milben, Acari. In: Brohmer P, Ehrmann P, Ulmer G (Eds) Die Tierwelt Mitteleuropas – Spinnentiere. Quelle & Meyer, Leipzig, 1–112.