Research Article |
Corresponding author: Cristina Fiera ( cristina.ivanescu@ibiol.ro ) Academic editor: Pavel Stoev
© 2016 Julia Shrubovych, Cristina Fiera.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Shrubovych J, Fiera C (2016) New records of Protura (Entognatha, Arthropoda) from Romania, with an identification key to the Romanian species. ZooKeys 552: 33-48. https://doi.org/10.3897/zookeys.552.6613
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The Romanian Protura were studied based on 175 specimens collected from Romania, along with bibliographic data. The main publication on the Romanian proturans was written by M.A.
Protura , Romania, distribution, ecology, remarks, identification key
Proturans are found world-wide except in the Arctic and Antarctic regions (
The first report on Romanian Protura was that of
The fauna of Romanian proturans is poorly known in contrast to some other European countries: Luxembourg, 30 species in 10 genera (
The aim of this study is to improve the study of this little known taxon in Romania by providing new records and distributional data on proturan species.
Protura were extracted from samples of leaf litter, soil and mosses in Berlese funnels. The material has been deposited in the Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków (ISEA). Specimens were mounted on slides in Marc Andre medium and were observed and identified with a phase-contrast microscope. In total 175 specimens from 22 sampling sites were examined. In our analysis we also considered the data taken from 17 Romanian collecting sites mentioned in the literature. Species were identified based on a key to European Protura (
The following abbreviations are used throughout the paper: pi – preimago, mj - maturus junior, LII - larva II, LI - larva I.
Chaetal nomenclature follows
Ilfov County: Periş, 44°40'18"N; 26°1'44"E, elevation 100 m, mixed forest, soil, 06. XI.2012.
Ilfov County: Jilava, 44°19'00.038"N; 26°03'54.579"E, elevation 50 m, mixed forest, soil, 02. XI.2013.
Prahova County: Valea Largă, 45°18'20.638''N; 25°34'19.943''E, elevation 770 m, beech forest, in dead trunk, 13.XI.2013.
Prahova County: Şotriile, 45°13'39''N; 25°43'44''E, elevation 600 m, located on the mountainside above the Doftana River, mountain beech forest with Luzula luzuloides (Lam.) Dandy and Wilmott, soil and litter, 12.XI.2013.
Prahova County: Voila, 45°09'58''N; 25°45'10''E, elevation 500 m, Quercus petraea (Mattuschka) Liebl. and Fagus sylvatica L. mixed forests, soil and litter, 12.XI.2013.
Prahova County: Cheile Brezei: 45°10'31.906"N; 25°41'16.153"E, elevation 455 m, shrubs and soil on rocks, 11.IX.2014.
Prahova County: Cheile Posadei, 45°17'39.947"N; 25°36'00.001"E, elevation 720 m, mosses and litter on rocks, 12.XI.2013.
Suceava County: Adam Peak, 47°30'58.17"N; 25°29'09.09"E, elevation 950 m, spruce forest, mosses on soil, 26.VIII.2014.
Suceava County: Iacobeni, 47°26'45.913"N; 25°18'41.182"E, elevation 915 m, Larix decidua Mill., soil, 26.VIII.2014.
Făgăraş Mountains: Valea Arpaşului, 45°40'52"N; 24°40'12"E, elevation 685 m, beech forest with Abies alba Mill. and Acer sp., soil, 10.IX.2014.
Făgăraş Mountains: Valea Arpaşului, 45°39'21.937"N; 24°40'13.930"E, elevation 850 m, forest with Abies alba and Fagus sylvatica L., soil, 10.IX.2014.
Făgăraş Mountains: Valea Arpaşului, 45°40'01.337"N; 24°40'15.289"E, elevation 820 m, harvested forest with Abies alba, soil, 10.IX.2014.
Făgăraş Mountains: Valea Brescioarei, 45°39'16.773"N; 24°53'09.207"E, elevation 930 m, spruce forest, 31.VII.2014.
Bârgău Mountains: Lunca Ilvei, 47°19'37.267"N; 25°58'20.189"E, elevation 785 m, forest with Abies alba and Fagus sylvatica, soil, 28.VIII.2014.
Bârgău Mountains: Piatra Fântânele, 47°17'27.107"N; 24°59'45.294"E, elevation 915 m, spruce forest, soil, 28.VIII.2014.
Bârgău Mountains: Valea Străjii, 47°13'05.330"N; 24°53'36.495"E, elevation 800 m, beech forest mixed with spruce, soil, 29.VIII.2014.
Bârgău Mountains: Tureac, 47°15'26.614"N; 24°50'54.282"E, elevation 795 m, meadow, soil, 29.VIII.2014.
Vălcea County: Voineasa, 45°25'00"N; 23°57'20"E, elevation 705 m, beech forest, soil, 01.XI.2012.
Cozia National Park: Călineşti Valley near Brezoi, 45°19'48"N; 24°14'30"E, elevation 610 m, litter of beech forest mixed with Pinus sp., 21.X.2012.
Olt Valley: Malaia, 45°21'11.18"N, 24°01' 11.74' E, elevation 495 m, beech forest, litter near rocks, 01.XI.2012.
Giurgiu County: Călugăreni, 44°10'48.839"N, 26°00'42.400"E, elevation 70 m, mixed forest, soil, 03.XI.2013.
Constanţa County: Cernavoda, 44°20'11.92'N, 28°01'05.211''E, elevation 55 m, shrubs, soil, 10.XI.2012.
Twenty-two species of Protura were identified in this study based on our sampling material and 15 new records for Romanian proturan fauna were established. In total, 27 species belonging to 6 genera and 4 families (Hesperentomidae, Protentomidae, Acerentomidae and Eosentomidae) are now recorded from Romania.
Some data about ecology, distribution in Europe and in Romania are given for each species and, where appropriate, remarks are provided. An identification key to the Romanian Protura species follows the species accounts.
1. Ionescuellum carpaticum (Ionescu, 1930)
Ecology. This species prefers dry to moderately humid rock mountain biotopes (
Distribution in Romania. This species was hitherto known only from Sinaia, Prahova County; lives under rocks in the forest (
Distribution in Europe. Austria (
2. Proturentomon minimum (Berlese, 1908)
Material examined. Site 1, 2 females.
Ecology. Usually occurs in grasslands (
Distribution in Romania. This species is known only from Periş forest (Ilfov County) (this study).
Distribution in Europe. Austria, Bosnia and Herzegovina, Czech Republic, Italy, Germany, Great Britain, Greece, Luxembourg, Poland, Portugal, Switzerland, Slovakia, European Russia (
Remarks. New record for the Romanian fauna.
3. Proturentomon sp.
Material examined. Site 3, one female.
Remarks. Probably a new species, more material is needed for description. This species has four anterior setae on tergites II‒VI (A1 and A2), whereas 10 Proturentomon species have only two anterior setae (A1) and three species lack them entirely.
4. Acerentulus alni Szeptycki, 1991
Material examined. Site 8, 2 females; site 3, 3 females; site 4, 3 females, 2 mj; site 10, one male, 1 pi; site 11, one female, 1 mj; site 13, 2 females, one male, 1 pi, 4mj; site 15, 2 females, one male 1LII.
Ecology. Lives in various types of forests and meadows (
Distribution in Romania. Adam Peak, Prahova County: Valea Largă, Şotriile, Făgăraş Mountains: Valea Arpaşului and Valea Brescioarei, Bârgău Mountains (this study).
Distribution in Europe. Poland, Slovakia (
Remarks. New record for the Romanian fauna.
5. Acerentulus confinis (Berlese, 1908)
Ecology. Eurytopic species; previously recorded from soil, litter and mosses of both humid and xerothermic forests, in garden soil, in mosses on limestone rocks, in detritus, and along the Black Sea coast (
Distribution in Romania. Sinaia (Prahova County) and in Pantelimon forest, near Bucharest (Ilfov County), in litter; Agigea (Constanţa County), in litter of Crataegus bushes, on dunes from zoological station at Agigea (
Distribution in Europe. Austria, Belgium, Bosnia and Herzegovina, Great Britain, Bulgaria, Corsica, Czech Republic, France, Germany, Greece, Hungary, Ireland, Italy, Portugal, Slovakia, Switzerland (
6. Acerentulus cf. confinis
Material examined. Site 18, one female, one male.
Remarks. Probably a new species or an intrapopulation variation. Our species differs from A. confinis (Berlese, 1908) in the absence of setae P3a on tergite VII and in a longer foretarsus (135 µm versus 100 µm in A. confinis).
7. Acerentulus exiguus Condé, 1944
Material examined. Site 5, one female; site 10, one male; site 12, 3 females, one male; site 13, one female.
Ecology. Eurytopic species; abundant in soil, litter, mosses, decaying wood and plant-debris of forests, meadows, xerothermic grasslands and shrubs on rocks (
Distribution in Romania. Voila forest (Prahova County), Făgăraş Mountains: Valea Arpaşului and Valea Brescioarei (this study).
Distribution in Europe. Greek mainland and Ukraine (
Remarks. New record for the Romanian fauna.
8. Acerentulus halae Szeptycki, 1997
Material examined. Site 6, one female, one male.
Ecology. Xerophilous species; the species was found in plant debris in meadow-steppes (
Distribution in Romania. This species was reported only from Adam Peak (this study).
Distribution in Europe. Known only from Ukraine (
Remarks. New record for the Romanian fauna.
9. Acerentulus traegardhi Ionescu, 1937
Material examined. Site 6, one male.
Ecology. Xerophilous species; abundant in soil and litter of forests, meadow-steppes and green patches inside cities squares (
Distribution in Romania. Comarova, near Black Sea, south of Agigea (Constanţa County), litter in forest (
Distribution in Europe. Austria, Belgium, Bosnia and Herzegovina, Bulgaria, Denmark, France, Germany, Greece, Hungary, Ireland, Italy, Poland, Slovakia, Spain, Sweden and Ukraine; some records from Europe have been misidentified as A. insignis and should be confirmed, especially from western Europe (
10. Acerentulus xerophilus Szeptycki, 1979
Material examined. Site 9, 2 females, 2 males.
Ecology. Xerophilous species; reported from soil and litter of forests, meadow-steppes, dry grasslands and city squares (
Distribution in Romania. This species is known only in Iacobeni (Suceava County) (this study).
Distribution in Europe. Poland and Ukraine (
Remarks. new record for the Romanian fauna.
11. Acerentulus sp. cunhai -group
Material examined. Site 20, one female.
Remarks. The Romanian specimen belongs to the cunhai-group according to Nosek's criteria (
Distribution in Romania. Malaia (Olt Valley) (this study).
Remarks. New record for the Romanian fauna.
12. Acerentomon affine Bagnall, 1912
Material examined. Site 7, one female, one male.
Ecology. This species prefers forest biotopes (
Distribution in Romania. Calafat (Dolj County) and Ciocăneasa, in forest litter (
Distribution in Europe. Austria, Bosnia and Herzegovina, Great Britain, France, Germany, Ireland, Luxembourg, Spain and Sweden (
13. Acerentomon carpaticum Nosek, 1961
Material examined. Site 3, one female, 1 mj, 2 LII; site 16, 1 mj, site 17, 5 females, 2 males.
Ecology. This species prefers forest biotopes (
Distribution in Romania. Valea Largă (Prahova County); Bârgău Mountains (Valea Străjii and Tureac) (this study).
Remarks. New record for the Romanian fauna.
Distribution in Europe. Ukraine (
14. Acerentomon mesorhinus Ionescu, 1930
Ecology. Reported from forest and meadow biotopes (
Distribution in Romania. Cumpatul - Sinaia (Prahova County, beech forest (
Distribution in Europe. Germany, Slovakia, Ukraine; Serbia (
15. Acerentomon microrhinus Berlese, 1909
Ecology. This species prefers forest biotopes (
Distribution in Romania. Parang Mountains, forest humus, 1000 m elevation, and in litter of oak forest in Pantelimon, near Bucharest (Ilfov County) (
Distribution in Europe. Austria, Bosnia and Herzegovina, Corsica, France, Italy, Slovakia, Slovenia and Ukraine (
16. Acerentomon quercinum Ionescu, 1932
Material examined. Site 20, one female, 1 mj.
Ecology. Prefers forest biotopes (
Distribution in Romania. Forest humus, Cernica (Ilfov County) and litter of oak forest, Ciocăneasa (
Distribution in Europe. Austria, Bosnia and Herzegovina, Hungary, Slovakia, Poland and Ukraine (
17. Acerentomon cf. quercinum
Material examined. Site 19, 3 males, 4 females, 1 mj.
Remarks. These specimens probably represent an undescribed species. Acerentomon cf. quercinum differs from A. quercinum
18. Acerentomon rostratum Ionescu, 1951
Material examined. Site 18, 2 females, 4 males, 1 LII, 1 LI.
Ecology. Lives in oak forest (
Distribution in Romania. Bumbeşti-Piţic, Copaci forest, oak forest (Gorj County) (
Distribution in Europe. Known only from Romania (
19. Acerella muscorum (Ionescu, 1930)
Ecology. This species prefers forest ecosystems (
Distribution in Romania. Sinaia-Cumpătul (Prahova County), in mosses (
Distribution in Europe. Germany (
20. Eosentomon armatum Stach, 1926
Material examined. Site 9, 2 mj; sit 21, 2 females.
Ecology. In soil and litter of forests (beech-hornbeam and oak) (
Distribution in Romania. Ponicova (Cazane) (Mehedinți County), in forest humus; in mosses on soil in Sinaia (Prahova County); Snagov (Ilfov County), Săbăreni, Tăgădău and Galaleu (Arad County) and under bark of Jepi, at 2000 m elevation near Caraiman Peak, Bucegi Mountains (
Distribution in Europe. Austria, Belgium, Czech Republic, Denmark, France, Great Britain, Iceland, Germany, Luxembourg, Poland, Portugal, Slovakia, Spain, Switzerland and Ukraine. All data before 1986 needs verification as some records are mixed with similar species (
21. Eosentomon carpaticum Szeptycki, 1985
Material examined. Site 4, 2 females; site 5, 2 females, one male, 1 LI; site 7, one male, 1 mj; site 14, 1 mj; site 19, one female, 2 males; site 20, one female.
Ecology. Found in soil and moss in beech forest and under dense overgrown shrubs (
Distribution in Romania. Şotriile, Cheile Posadei and Voila (Prahova County); Bârgău Mountains: Lunca Ilvei; Cozia National Park: Călineşti Valley; Olt Valley: Malaia (this study).
Distribution in Europe. Endemic Carpathian species, Ukraine and Poland (
Remarks. New record for the Romanian fauna.
22. Eosentomon enigmaticum Szeptycki, 1986
Material examined. Site 3, one female, 2 males, 1 LII.
Ecology. This species prefers forest ecosystems (
Distribution in Romania. Known only from Valea Largă (Prahova County) (this study).
Distribution in Europe. Poland and Ukraine (
Remarks. New record for the Romanian fauna.
23. Eosentomon pinetorum Szeptycki, 1984
Material examined. Site 1, 4 females, one male; site 4, one male; site 10, 3 females; site 12, 1 mj.
Ecology. Xerophilous species; lives in beech, oak, hornbeam, pine forests and mixed forests with spruce, thermophilous fir forest and steppe localities (Szeptycki 1998,
Distribution in Romania. Periş (Ilfov County); Şotriile (Prahova County); Făgăraş Mountains: Valea Arpaşului (this study).
Distribution in Europe. Austria, Czech Republic, Germany, Poland and Ukraine (
Remarks. New record for the Romanian fauna.
24. Eosentomon semiarmatum Denis, 1927
Material examined. Site 22, 3 females.
Ecology. Eurytopic species; reported from different forest and steppe localities (
Distribution in Romania. Forest litter from Sinaia (Prahova County); Cernica (Ilfov County) and Snagov (Ilfov County) (
Distribution in Europe. Balearic Islands, France, Germany, Poland and Ukraine (
25. Eosentomon silvaticum Szeptycki, 1986
Material examined. Site 3, one male, 1 LII.
Ecology. The species prefers woodlands (fir and mixed forests with fir, pine, beech, hornbeam) (
Distribution in Romania. This species is known only from Valea Largă (Prahova County) (this study).
Distribution in Europe. Poland and Luxembourg (
Remarks. New record for the Romanian fauna.
26. Eosentomon stachi Rusek, 1966
Material examined. Site 2, 3 females, one male, 2 mj.
Ecology. Xerophilous species; lives in different forests, meadows, dry pasture ground and in petrophilous turf on limestone (
Distribution in Romania. This species is known only from Jilava forest (Ilfov County) (this study).
Remarks. New record for the Romanian fauna.
Distribution in Europe. Austria, Luxembourg, Poland, Slovakia and Ukraine (
27. Eosentomon transitorium Berlese, 1908
Material examined. Site 5, one female, 1 mj; site 7, one female; site 11, one male, 1 LII.
Ecology. Eurytopic species; it has been recorded from soil and litter of various forests, town parks, alpine bushes Salix herbacea L. and Dryas octopelata L., in debris of tall herbs on rock shelves, dry meadows, grasslands, in xerothermic turf, in deep soil under stones (
Previous records: Sinaia (Prahova County), in beech litter; Agigea, near zoological station (Constanţa County), in litter, Acacia forest (
Distribution in Europe. Latvia, Estonia; most of these records should be confirmed (
1 | Spiracles present on meso- and metanota (Eosentomata, Eosentomon) | 2 |
– | Spiracles absent (Acerentomata) | 9 |
2 | Head setae aa and ap present, notal setae P2a and P3a of equal length (E. delicatum-group) | 3 |
– | Head setae aa absent, ap present, notal seta P2a shorter than P3a (E. transitorium-group) | 5 |
3 | Foretarsal sensillum c' on the line α6-δ5 | Eosentomon carpaticum |
– | Foretarsal sensillum c' proximal to the line α6-δ5 | 4 |
4 | Sensillum c' broadened, P1a on tergite VIII without basal dilation, notal seta P2a longer than P3a | Eosentomon stachi |
– | Sensillum c' slender, P1a on tergite VIII with basal dilation, notal seta P2a the same length as P3a | Eosentomon armatum |
5 | Seta P1a on tergite VII at the same level as P2 | 6 |
– | Seta P1a on tergite VII posterior to level of P2 | 7 |
6 | Seta D2 on metatarsus slender | Eosentomon transitorium |
– | Seta D2 on metatarsus spine-like | Eosentomon enigmaticum |
7 | Sternites IX–X with 6 setae | Eosentomon pinetorum |
– | Sternites IX–X with 4 setae | 8 |
8 | Seta P1a on tergites II–IV longer than P1, foretarsal sensillum t3 longer than c' | Eosentomon semiarmatum |
– | Seta P1a on tergites II–IV shorter than P1, foretarsal sensillum t3 same length as c' | Eosentomon silvaticum |
9 | Abdominal appendages I–II two-segmented | 10 |
– | Abdominal appendage I two-segmented, appendage II not segmented (Acerentomidae) | 12 |
10 | Tergites II–VII with 4 pairs of A-setae, calyx of maxillary gland small | Ionescuellum carpaticum (Hesperentomidae) |
– | Tergites II–VII with at most 2 pairs of A-setae, calyx of maxillary gland enlarged (Protentomidae) | 11 |
11 | Tergites II–VI with one pair of A-setae, foretarsal sensillum b about 2/3 length of c | Proturentomon minimum |
– | Tergites II–VI with 2 pairs of A-setae, foretarsal sensillum b nearly as long as c | Proturentomon sp. |
12 | Meso- and metanota with 2 pairs of A-setae, abd. appendages II–III each with 3 setae…14 | Acerentulus |
– | Meso- and metanota with 3 or 4 pairs of A-setae, abd. appendages II–III each with 2 setae | 13 |
13 | Meso- and metanota both with 3 pairs of A-setae, foretarsal sensillum t1 filiform, calyx of maxillary gland with racemose appendices | Acerella muscorum |
– | Mesonotum with 3 pairs of A-setae, metanotum with 4 pairs of A-setae, sensillum t1 claviform, calyx of maxillary gland smooth (Acerentomon) | 21 |
14 | Sternite XI with 4 setae | 15 |
– | Sternite XI with 6 setae | 16 |
15 | Foretarsal sensillum b very long, nearly reaching the base of claw, tergite VII with seta P3a | Acerentulus traegardhi |
– | Foretarsal sensillum b shorter, nearly equal to length of sensillum c, tergite VII without seta P3a | Acerentulus halae |
16 | Foretarsal sensillum a short, passing the base of sensillum t2 (A. cunhai-group) | Acerentulus sp. |
– | Foretarsal sensillum a long, nearly reaching or surpassing the base of seta γ3 (A. confinis-group) | 17 |
17 | Tergite VII with 3 pairs of A-setae | 18 |
– | Tergite VII with 4 pairs of A-setae | 19 |
18 | Tergite VII with seta P3a, sternite VII with seta Pc | Acerentulus xerophilus |
– | Tergite VII without seta P3a, sternite VII without seta Pc | Acerentulus exiguus |
19 | Tergite VII with seta P3a | 20 |
– | Tergite VII without seta P3a | Acerentulus cf. confinis |
20 | Maxillary sensilla spindle-shaped, foretarsal sensillum a' slender, sternites II-III without pores | Acerentulus confinis |
– | Maxillary sensilla parallel-sided and slender, foretarsal sensillum a' thickened, sternites II-III with pores | Acerentulus alni |
21 | Tergite VII with pair of x-setae(A. doderoi-group) | Acerentomon rostratum |
– | Tergite VII without x-setae | 22 |
22 | Sternite VIII with a pair of posterior setae (A. affine-group) Acerentomon affine | |
– | Sternite VIII without posterior setae (23) | A. microrhinus- group |
23 | Labrum slightly protruded (LR = 9), foretarsal sensilla a, b and c of the same length | Acerentomon microrhinus |
– | Labrum clearly protruded (LR = 7 or less), sensilla a, b and c of unequal lengths | 24 |
24 | Foretarsal sensillum b broadened | 25 |
– | Foretarsal sensillum b slender | 26 |
25 | Foretarsal sensillum a long, reaching the base of seta γ3, maxillary sensilla spindle-shaped, labial sensillum slender | Acerentomon quercinum |
– | Foretarsal sensillum a short, slightly surpassing the base of seta γ2, maxillary sensilla parallel-sided, labial sensillum broadened | Acerentomon cf. quercinum |
26 | Foretarsal sensillum a longer than c, sternite VI with 5 A-setae | Acerentomon carpaticum |
– | Foretarsal sensillum a shorter than c, sternite VI with 7 A-setae | Acerentomon mesorhinus |
The authors are very grateful to Mircea Fiera and Claudiu Avramescu (IBB) for field assistance and to Dr Alexandru-Ionuţ Petrişor for providing the map. Sincere gratitude goes to Prof. Ernest C. Bernard for English corrections and remarks and to anonymous reviewers and to editor for their constructive comments.